Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Molecular cloning and expression analysis of a ferritin H subunit from rock bream, Oplegnathus fasciatus

돌돔 ferritin H 유전자의 클로닝과 발현 분석

  • Kwon, Mun-Gyeong (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Jeong, Ji-Min (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Ju-Won (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Chan-Il (Pathology Division, National Fisheries Research and Development Institute)
  • 권문경 (국립수산과학원 병리연구과) ;
  • 정지민 (경상대학교 해양생명과학과) ;
  • 김주원 (경상대학교 해양생명과학과) ;
  • 박찬일 (경상대학교 해양생명과학과)
  • Received : 2013.10.07
  • Accepted : 2013.12.09
  • Published : 2013.12.31
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Abstract

Ferritin is an evolutionarily conserved protein that plays an important role in iron storage and detoxification. In this study, the gene encoding a ferritin H subunit homologue (RbFH) was cloned from rock bream (Oplegnathus fasciatus) and analyzed at the expression. The full-length ferritin H cDNA was 1162 bp long and contained an open reading frame (ORF) of 531 bp that encoded 177 amino acid residues with a predicted molecular mass of 20.8 kDa. The 5' UTR was 297 bp in length, and the 3' UTR 298 bp, and preceded by a 5'-untranslated region that contains a putative Iron Regulatory Element (IRE). The deduced amino acid sequence of RbFH shares extensive sequence identities with the H ferritins of a number of fish species and contains the ferroxidase center that is preserved in ferritin H subunits. Examination of tissue specific expression indicated that RbFH expression was most abundant in PBLs, RBC, liver and muscle.

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References

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