• The Korean Journal of Food And Nutrition
• /
• v.10 no.2
• /
• pp.268-271
• /
• 1997

Human mitochondrial aldehyde dehydrogenase(ALDH2) is mainly responsible for the oxidation of acetaldehyde generated during alcohol oxidation in vivo. To investigate the role of ALDH2 in alcohol metabolism, it was needed to get solubilized enzyme. The cDNA of ALDH2 is isolated from cDNA library and ligated to several expression vectors for E. coli. At almost expression system to be constructed, the broad expression band of ALDH2 was detected. But, the large part of the expressed protein consisted as inclusion body, the yield of solubilized enzyme was not more tan 5% of the total expressed amount. Recombinant ALDH2 was verified from the several expression systems.

• Korean Journal Plant Pathology
• /
• v.11 no.1
• /
• pp.66-72
• /
• 1995

The coat protein (CP) gene of cucumber mosaic virus-As (CMV-As) strain was engineered for expression in the plant by using the cauliflower mosaic virus 35S transcript regulatory sequences. The CP gene was cloned into an Agrobacterium-derived binary vector. A chimeric gene was constructed by the cDNA of CMV-As CP and plant expression vector pBI121. The clone, pCMAS66, was first introduced into the phagemid vector pSPORT1 for situating sense orientation for translation and making restriction sites in order to re-introduce plant expression vector, pHI121. The resulting subclone pCASCP02 and plant expression vector pBI121 were treated with BamHI-SacI for excising the target gene and removing GUS gene, respectively. After Agrobacterium transformation by freeze-thaw technique, the clone, pCMASCP121-123 which contains sense orientation of the target gene, was selected and confirmed by restriction endonuclease analysis. The CMV-As CP gene was introduced into A. tumefaciens. The results on tobacco plant transformation with the vector system revealed that the system could be successfully introduced and showed high frequency of selection to putative transformations.

• Korean Institute of Interior Design Journal
• /
• v.15 no.2 s.55
• /
• pp.73-80
• /
• 2006

The purpose of this study is to analyze the characteristics of structural expression in the works of Louis I. Kahn. Kahn's main architectural thoughts of 'what it wants to be' and 'how it was done' are important in this research because these statements act as a clue to understand the relationship between structural expression and space. 'What it wants to be' means the essence and existence of architecture and 'how it was done' shows the tectonic of architecture and construction process to enhance its presence. Kahn's emphasis of structural expression stems from the fact that structure is the logical part of whole building system rather than the concealed object for the exterior of buildings. Therefore structure of Kahn's architecture is not a structure only to support building but a mean to express his thoughts in a corporeal way. In Kahn's works, the characteristics of structural expressions are summarized as the visualization of the order in dynamics, construction process, spatial system, and the relationship between structure and light in the space.

• YAKHAK HOEJI
• /
• v.49 no.1
• /
• pp.86-91
• /
• 2005

Flavonoids are class of polyphenolic compounds widely distributed in the plant kingdom, which display a variety of biological activities, including antiviral, antithrombotic, antiinflammatory, antihistaminic, antioxidant and free-radica 1 scavenging abilities. The antioxidant enzyme (AOE) system plays an important role in the defense against oxidative stress insults. To determine whether flavonoid, myricetin can exert antioxidative effects not only directly by modulating the AOE system but also scavenging free radical, we investigated the influence of the flavonoid myricetin on cell viability, different antioxidant enzyme activities, ROS level and the expression of different antioxidant emzyme in B16F10 murine melanoma cells. Myricetin in a concentration range from 6.25 to $50\;{\mu}M$ decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities, but catalase (CAT) activity was increased. In the myricetin-treated group, ROS levels were decreased dose-dependently. Antioxidant enzyme expression was measured by RT-PCR. Myricetin treatment of B16F10 cells increased catalase expression. Expression levels of copper zinc superoxide dismutase (CuZn SOD) were not affected by exposure of myricetin. Manganese superoxide dismutase (Mn SOD) and GPx expression levels decreased slightly after myricetin treatment. In conclusion, the antioxidant capacity of myricetin was due to CAT and free-radical scavenging.

• Journal of Pharmacopuncture
• /
• v.24 no.2
• /
• pp.68-75
• /
• 2021

Objectives: The hair follicle is composed of more than 20 kinds of cells, and mesoderm derived dermal papilla cells and keratinocytes cooperatively contribute hair growth via Wnt/β-catenin signaling pathway. We are to investigate β-catenin expression and regulatory mechanism by CBD in alopecia hair tissues and dermal papilla cells. Methods: We performed structural and anatomical analyses on alopecia patients derived hair tissues using microscopes. Pharmacological effect of CBD was evaluated by β-catenin expression using RT-PCR and immunostaining experiment. Results: Morphological deformation and loss of cell numbers in hair shaft were observed in alopecia hair tissues. IHC experiment showed that loss of β-catenin expression was shown in inner shaft of the alopecia hair tissues, indicating that β-catenin expression is a key regulatory function during alopecia progression. Consistently, β-catenin expression was decreased in testosterone or PMA treated dermal papilla cells, suggesting that those treatments are referred as a model on molecular mechanism of alopecia using dermal papilla cells. RT-PCR and immunostaining experiments showed that β-catenin expression was decreased in RNA level, as well as decreased β-catenin protein might be resulted from ubiquitination. However, CBD treatment has no changes in gene expression including β-catenin, but the decreased β-catenin expression by testosterone or PMA was restored by CBD pretreatment, suggesting that potential regulatory effect on alopecia induction of testosterone and PMA. Conclusion: CBD might have a modulating function on alopecia caused by hormonal or excess of signaling pathway, and be a promising application for on alopecia treatment.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.19 no.6
• /
• pp.821-827
• /
• 2009

Human emotion can be reflected by their facial expressions. So, it is one of good ways to understand people's emotions by recognizing their facial expressions. General recognition system of facial expressions had selected interesting points, and then only extracted features without analyzing physical meanings. They takes a long time to find interesting points, and it is hard to estimate accurate positions of these feature points. And in order to implement a recognition system of facial expressions on real-time embedded system, it is needed to simplify the algorithm and reduce the using resources. In this paper, we propose a real-time recognition algorithm of facial expressions that project the grid points on an expression space based on Gabor wavelet feature. Facial expression is simply described by feature vectors on the expression space, and is classified by an neural network with its resources dramatically reduced. The proposed system deals 5 expressions: anger, happiness, neutral, sadness, and surprise. In experiment, average execution time is 10.251 ms and recognition rate is measured as 87~93%.

• Korean Journal of Cognitive Science
• /
• v.14 no.1
• /
• pp.41-50
• /
• 2003

We tested the capability of the pleasure and arousal dimension-based facial expression synthesis system and proposed the most effective interface for it. First, we tried to confirm the adequateness of the dimensional model as a basic structure of the internal states for the system. Fer it, subjects compared the 17 facial expressions on the two axes. The results validated the fundamental hypothesis of the system. Second, we chose 21 representative expressions from the system to test its performance and had subjects rate their similarities. We analyzed these data using multidimensional scaling methods and these results verified the system's reliability. Third, we compared the efficiencies of two interfaces -coordinate values and slide bars- to find the most suitable interface for the system. Subjects synthesise 25 facial expressions with each interface of it. The results showed that the visualization of two dimensional values into Cartesian coordinate is more stable as an input display of facial expression synthesis system based on dimensions.

• Microbiology and Biotechnology Letters
• /
• v.32 no.1
• /
• pp.11-15
• /
• 2004

Embryonic stem (ES) cells are derived from the inner cell mass of the blastocyst-stage embryos that can be propagated indefinitely and, at the same time, can be differentiated into all the cell types that constitute the body. Current research using ES cells is mainly focused on the efficient generation of specific cell types by employing optimal differentiation conditions, which often requires the genetic manipulation of ES cells. As a way of developing an efficient system to regulate foreign gene expression in ES cells, we have inserted the gene encoding Corynebacterium diphtheria toxin-A (DTA) into an autonomously induced plasmid under positive doxycycline control ('Tet-on' system). In this study, we demonstrate that this system can lead to the cell death of mouse ES cells by the induction of DTA expression when exposed to the tetracycline derivative, doxycycline. MTT assay showed that this induction resulted in the apoptosis of ES cells.

• Journal of Ocean Engineering and Technology
• /
• v.13 no.3B
• /
• pp.106-115
• /
• 1999

Modeling a discrete event system such as a sequential control system is difficult compared with a continuous system. Petri nets have been introduced as an analyzing and design tool for the discrete systems. One of the problems in its applications is that the model can not be analyzed easily in the case of large scale or complicated systems because of increase of the number of components of the system. To overcome this problem, some methods for dividing or reducing Petri nets have been suggested. In this paper, an approach for a hierarchical expression of Petri nets based on Sequential Function Chart(SFC) is proposed. A measuring tank system will be described as a typical kind of discrete systems. The system is modeled by sub Petri nets based on SFC in order to analyze and visualize efficiently about the dynamic behaviors of the system. Some numerical simulations using state equations are performed to prove the validity of the proposed method.

• Journal of fish pathology
• /
• v.16 no.3
• /
• pp.147-152
• /
• 2003

The CMV promoter driven luciferase reporter gene coding plasmid (pcDNA-luc) was constructed and used as a model for DNA immunization study. Expression of the recombinant luciferase protein was confirmed in vitro in RTG-2 cell line before using in vivo study in olive flounder. In dose response study, the maximum expression of the luciferase gene was found in the group injected with 10-15μg of plasmid DNA. The kinetic study showed that the luciferase gene expression was reached at the maximum level at one day after injection and slightly decreased after then but significantly high level of expression was sustained until the conducted experiment of 7 days. In the study of tissue distribution of gene expression, it was found that luciferase gene was expressed at the significant level in immune organs such as gill and spleen, located far from the injected site, suggesting the systemic distribution of the intramuscularly injected DNA in olive flounder.