• Title/Summary/Keyword: exponential growth

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Effects of Jasmonic Acid-Cellulase Combined Elicitors on the Paclitaxel Production in Suspension Cultures of Taxus wallichiana Zucc (Taxus wallichiana Zucc. 현탁세포에서 Jasmonic Acid-Cellulase 복합 Elicitor가 Paclitaxel 생합성에 미치는 영향)

  • Hoi, Nguyen Ngoc;Luong, Hoang Van;Long, Nguyen Van;Duong, Vu Binh;Byun, Sang-Yo
    • KSBB Journal
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    • v.25 no.2
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    • pp.193-198
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    • 2010
  • Cell cultures of Taxus wallichiana Zucc. were made to enhance the production of anticancer agent paclitaxel. In suspension cultures, the maximum cell growth rate in exponential growth phase was 0.14 $day^{-1}$ which was correlated to 4.96 days of cell doubling time. The production of paclitaxel was non-growth associated. The paclitaxel production was started after the exponential growth phase and increased to declined phase where the maximum concentration was observed. Various elicitors were tested to enhance the production of paclitaxel. The combination of two elicitors of jasmonic acid and cellulase increased the production of paclitaxel 1.8 and 3.1 times compared to paclitaxel production by individual elicitor respectively.

Effects of Various Intensities and Wavelengths of Light Emitting Diodes (LEDs) on the Growth of the Prasinophytes Tetraselmis suecica and T. tetrathele (담녹조강 Tetraselmis suecica 및 Tetraselmis tetrathele의 생장에 미치는 발광다이오드(Light-Emitting Diodes; LEDs) 광량과 파장의 영향)

  • Han, Kyong Ha;Oh, Seok Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.1
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    • pp.64-71
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    • 2018
  • This study was conducted to investigate the effects of light intensity and wavelength on the growth of Tetraselmis suecica and Tetraselmis tetrathele. These species were exposed to a blue light-emitting diode (LED; max=450 nm), a yellow LED (max=590 nm), a red LED (max=630 nm) and a fluorescent lamp (three wavelengths). The maximum growth rates (${\mu}_{max}$) of T. suecica and T. tetrathele under a red LED were 1.12/day and 0.95/day, respectively. Under a yellow LED, growth rates were 70% of the values for red wavelength, with low half-saturation constants (Ks). The optimum light source to ensure economically effective and productive growth in a Tetraselmis culture system (Photo-Bioreactor) would thus appear to be a three-phase culture, wherein a yellow LED is used during the lag phase and initial exponential phase to increase growth rate, followed by a red LED during the middle exponential phase to maximize growth rate, and finally a yellow LED again during the late exponential phase and stationary phase to achieve increased yield of useful bioactive substances.

The Effects of Fertilization on Growth Performances and Physiological Characteristics of Liriodendron tulipifera in a Container Nursery System (시비 처리가 백합나무 용기묘의 생장 및 생리적 특성에 미치는 영향)

  • Cho, Min Seok;Lee, Soo Won;Park, Byung Bae;Park, Gwan Su
    • Journal of Korean Society of Forest Science
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    • v.100 no.2
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    • pp.305-313
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    • 2011
  • Fertilization is essential to seedling production in nursery culture, but excessive fertilization can contaminate surface and ground water around the nursery. The objective of this study was to find optimal fertilization practice of container seedling production for reducing soil and water contamination around the nursery without compromising seedling quality. This study was conducted to investigate growth performance, photosynthesis, chlorophyll fluorescence, and chlorophyll contents of Liriodendron tulipifera growing under three different fertilization treatments (Constant rate, Three-stage rate, and Exponential rate fertilization). Root collar diameter, height, and biomass of L. tulipifera were the highest at Constant treatment. Like growth performance, seedling quality index (SQI) were higher at Constant than at other treatments, but not significantly different among treatments. L. tulipifera showed good photosynthetic capacity at all treatments. Photochemical efficiency and chlorophyll contents were significantly lower at Exponential than at other treatments. Therefore, Exponential fertilization which is 50% fertilizer of other treatments would maximize seedling growth and minimize nutrient loss.

Genotypic Variation of Rapid Canopy Closure and Its Relationship with Yield of Rice (벼 조기초관폐쇄성의 품종 변이 및 수량과의 관계)

  • Fu, Jin-Dong;Lee, Byun-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.137-143
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    • 2008
  • Rapid canopy closure (RCC) is one of the physiological attributes that may enhance genetic yield potential of rice (Oryza sativa L.) in a growing season. Crop growth before canopy closure could be described by an exponential equation of $y\;=\;{\alpha}{\cdot}{\exp}({\beta}{\cdot}t)$ where $\alpha$ is the crop leaf area index (LAI) or shoot dry weight (DW), t is the thermal time, $\beta$ is the LAI or DW at the beginning of the exponential growth and is the relative growth rate of LAI ($m^2m^{-2}^{\circ}C^{-1}$) or DW($gg^{-2}^{\circ}C^{-1}$). Field experiment using 22 cultivars revealed that the exponential growth phase before canopy closure can be divided into two sections; an earlier section during which crop dry weight and LAI of varieties are highly dependent on $\alpha$ and a second section where crop dry weight and LAI are highly dependent on $\beta$. Grain weight had significantly positive correlation with $\alpha$ parameter and dry weight and LAI during early exponential phase. The parameter $\beta$ of the exponential growth curve had positive and significant correlation with the LAI and dry weight during the late exponential growth phase, grain number per unit area, and grain yield. There was genotypic difference for RCC parameters, $\alpha$ and $\beta$, indicating the possibility of genetic improvement for these traits.

Expression of c-jun by X-ray According to Cell Growth State in CaSki Cell Line (CaSki 세포주에서 성장 상태에 따른 X-선에 의한 c-jun의 발현)

  • Jang, Seong-Sun;Park, Woo-Yoon
    • Radiation Oncology Journal
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    • v.17 no.3
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    • pp.223-229
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    • 1999
  • Purpose : The expression pattern of c-jun by ionizing radiation according to cell growth state (exponential growth vs. stationary phase) and its relationship with cell cycle redistribution were investigated. Materials and Methods : The exponential growth phase (day 4) and stationary phase (day 9) cells were determined from cell growth curve according to the elapse of days in CaSki. The cells were irradiated using 6 MV X-ray with a dose of 2 Gy at a fixed dose rate of 3 Gy/min. Northern blot analysis was peformed with total cellular RNA and cell cycle distribution was analyzed using flow cytometry according to time-course after irradiation. Results : The maximum expression of c-jun occurred 1 hour after irradiation in both exponential growth and stationary phase cells. After then c-jun expression was elevated upto 6 hours in exponential growth phase cells, but the level decreased in stationary phase cells. Movements of cells from G0-G1 to S, G2-M phase after irradiation were higher in exponential growth phase than stationary phase. Conclusion : c-jun may be involved in the regulation of cellular proliferation according to the growth states after irradiation.

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Comparative Whole Cell Proteomics of Listeria monocytogenes at Different Growth Temperatures

  • Won, Soyoon;Lee, Jeongmin;Kim, Jieun;Choi, Hyungseok;Kim, Jaehan
    • Journal of Microbiology and Biotechnology
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    • v.30 no.2
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    • pp.259-270
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    • 2020
  • Listeria monocytogenes is a gram-positive, facultative anaerobe food pathogen responsible for the listeriosis that mostly occurs during the low-temperature storage of a cold cut or dairy products. To understand the systemic response to a wide range of growth temperatures, L. monocytogenes were cultivated at a different temperature from 10℃ to 42℃, then whole cell proteomic analysis has been performed both exponential and stationary cells. The specific growth rate increased proportionally with the increase in growth temperature. The maximum growth rate was observed at 37℃ and was maintained at 42℃. Global protein expression profiles mainly depended on the growth temperatures showing similar clusters between exponential and stationary phases. Expressed proteins were categorized by their belonging metabolic systems and then, evaluated the change of expression level in regard to the growth temperature and stages. DnaK, GroEL, GroES, GrpE, and CspB, which were the heat&cold shock response proteins, increased their expression with increasing the growth temperatures. In particular, GroES and CspB were expressed more than 100-fold than at low temperatures during the exponential phase. Meanwhile, CspL, another cold shock protein, overexpressed at a low temperature then exponentially decreased its expression to 65-folds. Chemotaxis protein CheV and flagella proteins were highly expressed at low temperatures and stationary phases. Housekeeping proteins maintained their expression levels constant regardless of growth temperature or growth phases. Most of the growth related proteins, which include central carbon catabolic enzymes, were highly expressed at 30℃ then decreased sharply at high growth temperatures.

Growth Inhibition of E. coli O157:H7 and Salmonella typhimurium by Lactic Acid Bacteria and Bifidobacteria (젖산균과 비피더스균에 의한 Escherichia coli O157:H7과 Salmonella typhimurium의 생장억제)

  • 김현욱;안영태;신필기
    • Journal of Food Hygiene and Safety
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    • v.12 no.3
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    • pp.181-187
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    • 1997
  • Lactobacillus acidophilus NCFM, Lactobacillus casei YIT 9018, Bifidobacterium longum 8001, and Bifidobacterium longum 8025 at the level of 106 cfu/$m\ell$ were cultured with 104 cfu/$m\ell$ of Escherichia coli O157:H7 KSC 109 or Salmonella typhimurium ATCC14028, in order to verify the effects of lactic acid bacteria and bifidobacteria on the growth of the pathogens. In the mixed culture of lactic acid bacteria with E. coli O157:H7 KSC 109, Growth inhibition and atypical microcolonies of E. coli O157:H7 KSC 109 were observed. The pathogens inoculated grew for 5 hors (pH 5.3), by the time L. acidophilus NCFM reached the exponential growth phase, and then the surviving pathogens were decreased to 101 cfu/$m\ell$ after 35 hours. When L. caseiYIT 9018 was grown with the pathogens, they grew for 10 hours (pH 4.6), by the time L. casei YIT 9018 reached the end of exponential growth phase, and then the surviving pathogens were decreased drastically. Up to the stationary growth phase of lactic acid bacteria, L. acidophilus NCFM exhibited stronger inhibition against the pathogens than L. casei YIT 9018 did, which might be attributed to its faster growth. Likewise bifidobacteria inhibited the growth of the pathogens tested, bifidobaceria was weaker in the inhibitory activity than lactic acid bacteria. When Bifidobacterium longum 8001 was cultured with the pathogens, E. coli O157:H7 KSC 109 was gradually ingibited at the stationary growth phase of bifidobacteria, atypical microcolonies were formed on Levine EMB medium after 48 hours, and Salmonella grew up to 106 dfu/$m\ell$, then was drastically ingibited at the exponential growth phage of Bifidobacterium longum 8001. But when Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same level of Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same lever of Bifidobacteriuam longum 8025 after 10 hours, then the surviving pathogens were decreased drastically.

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The Characteristics of Toxin Production in the Korean Toxic Cyanobacteria (국내산 유독 남조류의 독소생산 특성)

  • Kim, Hwa-Bin;Park, Hae-Kyung;Shin, Kyodong;Moon, Jeong-Suk
    • Journal of Korean Society on Water Environment
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    • v.26 no.5
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    • pp.834-840
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    • 2010
  • To find out the toxin production characteristics of Korean harmful cyanobacteria, we isolated 14 cyanobacterial strains from Korean lakes and rivers and analyzed the kinds and cellular content of microcystins (MCYSTs) of cyanobacterial isolates using cultured biomass. And we measured the MCYSTs production by growth phase of two representative toxic strains, Microcystis aeruginosa (HG-015) and Anabaena planktonica (HG-012). Among seven cyanobacteral species, Microcystis wesenbergii showed the highest cellular MCYSTs content. MCYST-RR was the most dominant toxin reaching more than 85% of MCYSTs produced by isolated cyanbacterial strains. During the mass culture, Microcystis aeruginosa (HG-015) showed the highest yield and accumulation of MCYSTs in the exponential growth phase. However the cellular content of chlorophyll a and MCYSTs of Anabaena planktonica (HG-012) showed higher value in the stationary and early death phase than in the exponential growth phase. Our results suggest that control and removal of harmful cyanobacterial bloom before exponential growth phase may be effective to prevent health risk of cyanobacterial toxins in the drinking water sources.

THE EXPONENTIAL GROWTH AND DECAY PROPERTIES FOR SOLUTIONS TO ELLIPTIC EQUATIONS IN UNBOUNDED CYLINDERS

  • Wang, Lidan;Wang, Lihe;Zhou, Chunqin
    • Journal of the Korean Mathematical Society
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    • v.57 no.6
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    • pp.1573-1590
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    • 2020
  • In this paper, we classify all solutions bounded from below to uniformly elliptic equations of second order in the form of Lu(x) = aij(x)Diju(x) + bi(x)Diu(x) + c(x)u(x) = f(x) or Lu(x) = Di(aij(x)Dju(x)) + bi(x)Diu(x) + c(x)u(x) = f(x) in unbounded cylinders. After establishing that the Aleksandrov maximum principle and boundary Harnack inequality hold for bounded solutions, we show that all solutions bounded from below are linear combinations of solutions, which are sums of two special solutions that exponential growth at one end and exponential decay at the another end, and a bounded solution that corresponds to the inhomogeneous term f of the equation.