• Title/Summary/Keyword: experiments

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Studies on the Various Utilization of Microbial Formulation for the Production of Vegetable Crops (원예작물(園藝作物) 생산성(生産性)에 미치는 미생물(微生物) 제제(製劑)의 복합적(複合的) 이용연구(利用硏究))

  • Kim, Kwang-Sik;Kim, Young-Woong;Choi, Young-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.2
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    • pp.191-205
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    • 1995
  • The carrier materials used for the development of bacterial inoculants to be effective in field were made with various carrier materials of two major forms, alginate bead and powder inoculants. Inoculants were prepared after mixing those carrier materials with Pseudomonas fluorescens SSL3 and Bacillus subtilis B5, and the treatment effects of each inoculants was investigated on cucumber, tomato, pepper and potato. Survival density of SSL3 and B5 in various carrier materials for duration of storage and the bead inoculants were better than the powder. In the powders, survival rate increased in carrier materials treated 5% skimilk. The growth condition of microorganisms in carrier materials is good at powder. When they were preserved in the long period, contamination is problem. Scanning(200 to 600nm) of the P. fluorescens SSL3 supernatant in centrifuged MKB broth incubated for 48h had two main peaks, pyochelin(300nm) and pyoverdin(400nm). The potato yield in field experiments of spring, treated with bead formulas showed increase of 22~29% in whole potato breeds as compared with control, because the bead formulas degraded, and released the antibiotic microorganisms in slow and constant rate. In the pot experiment, there were significant difference in soil, wheatbran, and bead formed wheatbran.

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Adsorption of Heavy Metal Cations by Fe and Al Hydroxides (철, 알루미늄 수산화물에 의한 중금속 Ion의 흡착)

  • Lee, Jyung-Jae;Chang, Sang-Moon;Choi, Jyung
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.2
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    • pp.105-113
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    • 1995
  • Adsorption experiments of heavy metal cations by Fe- and Al-hydroxides was conducted to obtain clear information on their adsorption mechanisms. The adsorption isothermal curves of heavy metal cations by Fe- and Al-hydroxides conformed to Langmuir's equation. Increasing the crystallinity degree of Fe- and Al-hydroxides tended to decrease the adsorption capacity and binding energy of heavy metal cations. At the same crystallinity degree, Al-hydroxide showed higher adsorption capacity and energy for the heavy metal cations than Fe-hydroxide. The adsorption capacity and energy of heavy metal cations were directly related to CEC, specific surface area and charge density of hydroxides, and the sequence was in the order of $Cu^{+{+}}$ > $Zn^{+{+}}$ > $Cd^{+{+}}$. The adsorption mechanism of $M^{+{+}}$ form of heavy metal could be presumed as the specific adsorption of $M^{+{+}}$ and the desorption of two $H^+$ from the surface aquo($OH_2$) and/or hydroxo(-OH) group for each mole of $M^{+{+}}$ adsorbed. A ring structure between $M^{+{+}}$ and two surface aquo and/or hydroxo groups was postulated. Nonspecific adsorption involved the adsorption of $MCl^+$ and the desorption of one H+ from the surface aquo and/or hydroxo groups for each mole of $M^{+{+}}$ adsorbed. A single bond structure in which $MCl^+$ replaced one $H^+$ from the surface aquo and/or hydroxo groups was postulated. The ratio of specific to nonspecific adsorption increased with increasing pH.

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Effects of Application Method of Pig Compost and Liquid Pig Manure on Yield of Whole Crop Barley (Hordeum vulgare L.) and Chemical Properties of Soil in Gyehwa Reclaimed Land (계화간척지에서 돈분뇨 퇴.액비 시용이 청보리 (Hordeum vulgare L.) 수량 및 토양화학성에 미치는 영향)

  • Lee, Sang-Bok;Cho, Kwang-Min;Baik, Nam-Hyun;Lee, Jung-Jun;Oh, Young-Jin;Park, Tail-Il;Kim, Kee-Jong
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.353-360
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    • 2011
  • In order to develop the application method of pig compost (PC) and liquid manure (LM) for whole crop barley cultivation, experiments were conducted at Munpo series (coarse loamy, mixed, nonacid, mesic family of Typic Fluvaquents) soil in Gyehwa-reclaimed land, six plots, a LM applied rate as N% ; non-application, chemical fertilizer (CF)100, 100, 50+50, 50+CF50 and (PC30+LM40)+LM50 as basal and additional fertilizer. $NO_3^-$-N content in soil was decreased as along with the growth of plant, highest in LM100% as basal fertilization at early growth stage and highest in (PC30%+LM40%)+LM40% and CF100% at last growth stage. Amount of $NO_3^-$-N and $NH_4^+$-N in soil was high in (PC30%+LM40%)+LM40% and CF100% of top soil but in subsoil significant difference was little in all treatment. Amount of OM, $A_V.P_2O_5$, T-N, exchangeable Ca and Na in soil was higher (PC30%+LM40%)+LM40% than non-application after harvest. Amount of nutrient uptake in plant was higher in CF100% and split application of LM than LM 100% application. Nitrogen utilization rate was in the order of CF100% >LM50%+LM50%=LM50%+CF50%>(PC30%+LM40%)+LM40% >LM100%. The yield of whole crop barley in (PC30%+LM40%)+LM40% and CF100% was 3.2 times more than in non-application ($309kg\;10a^{-1}$). Feed values such as crude protein and TDN was increased 1.0% ~ 1.4% in LM as split application than basal 100% treatment. Accordingly, in order to increase yield of a whole crop barley with application PC+LM in reclaimed land treat split application rather than to treat LM 100% into the land.

The Effect of Application Levels of Slurry Composting and Bio-filtration Liquid Fertilizer on Soil Chemical Properties and Growth of Radish and Corn (총각무와 옥수수 재배시 SCB액비 시용수준이 토양화학성과 생육에 미치는 영향)

  • Kang, Seong-Soo;Kim, Min-Kyeong;Kwon, Soon-Ik;Kim, Myong-Suk;Yoon, Sung-Won;Ha, Sang-Gun;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1306-1313
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    • 2011
  • A liquid fertilizer treated with slurry composting and biofiltration (SCB) process has been applied increasingly on agricultural field but the effects on the soil properties and crop production has not been throughly evaluated. This study was conducted to investigate the effect of the SCB application on soil chemical properties and the growth of radish and corn. SCB liquid fertilizer as a basal fertilization was treated with five levels based on $6kg\;10a^{-1}$ for radish and $10kg\;10a^{-1}$ for corn. The experimental design was the completely randomized block design with five levels and three replicates. Electrical conductivity (EC), $NO_3$-N, Exch. K and Exch. Na increased depending on the treatment levels of SCB. There were no changes in soil organic matter, Avail. $P_2O_5$, Exch. Ca and Exch. Mg. EC, $NO_3$-N and Exch. Na content decreased as precipitation increased. Especially, they decreased up to the initial condition before the treatment after the heavy rainy season in 2008. Although Exch. K decreased at the rainy season, they remained relatively higher content after the experiment on August, 2008. Fresh weight and the amount of N uptake of radish increased due to the levels of SCB, but corn did not present any significant increase. It is recommended that we need to decide the proper amount of SCB as well as the application method on the field to increase the productivity and decrease environmental stress. Additional experiments also need to clarify the effect of the trace element and heavy metal accumulations due to long term application of SCB.

Effect of Monoculture and Mixtures of Green Manure Crimson Clover (Trifolium incarnatum) on Rice Growth and Yield in Paddy (답리작에서 녹비작물 크림손클로버 단파 및 혼파가 벼 생육 및 수량에 미치는 영향)

  • Jeon, Weon-Tai;Seong, Ki-Yeong;Kim, Min-Tae;Oh, In-Seok;Choi, Bong-Su;Kang, Ui-Gum
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.847-852
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    • 2011
  • Green manure crops are mainly used to reduce the application of chemical fertilizers. Mixture of green manure crops have beneficial effects in agroecosystem. In this study, experiments were conducted to evaluate the effects of monoculture and mixtures of crimson clover (Trifolium incarnatum) on rice growth and yield in paddy. This experiment was conducted at Sinheung series (fine loamy, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts) from Oct. 2007 to Oct. 2009 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. Seeding rates of crimson clover (CC) were consisted of monoculture (CC2, 3, 4 kg and hairy vetch 5 kg $10a^{-1}$) and mixtures (CC 2 + barley 7, CC 3 + barley 7, CC 4 + barley 7, and CC2 + hairy vetch $5kg\;10a^{-1}$). Seeds were drilled by partial tillage machine on 9th Oct. in 2007. Monoculture and mixture of crimson clover as a green manure crop was incorporated in soil for rice cultivation on 15th May in 2008. Chemical fertilizers had not been applied to monoculture and mixture plots. The biomass and N production of monoculture plots were lower than mixture plots. The biomass and N production of CC 2 + hairy vetch $5kg\;10a^{-1}$ plot were the highest among mixtures treatments. In rice growing season, ammonium nitrogen concentrations in soil were a little high trends at CC 2 + hairy vetch $5kg\;10a^{-1}$ plot. And soil bulk density and porosity were improved at mixture plot after rice harvesting. The rice yield of CC 2 + hairy vetch $5kg\;10a^{-1}$ plot was not significantly different from conventional practice plot. These results indicated that cropping of crimson clover with hairy vetch mixture was better than barley mixture for environmental friendly rice cultivation.

Estimation of Nondestructive Rice Leaf Nitrogen Content Using Ground Optical Sensors (지상광학센서를 이용한 비파괴 벼 엽 질소함량 추정)

  • Kim, Yi-Hyun;Hong, Suk-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.6
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    • pp.435-441
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    • 2007
  • Ground-based optical sensing over the crop canopy provides information on the mass of plant body which reflects the light, as well as crop nitrogen content which is closely related to the greenness of plant leaves. This method has the merits of being non-destructive real-time based, and thus can be conveniently used for decision making on application of nitrogen fertilizers for crops standing in fields. In the present study relationships among leaf nitrogen content of rice canopy, crop growth status, and Normalized Difference Vegetation Index (NDVI) values were investigated. We measured Green normalized difference vegetation index($gNDVI=({\rho}0.80{\mu}m-{\rho}0.55{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.55{\mu}m)$) and NDVI($({\rho}0.80{\mu}m-{\rho}0.68{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.68{\mu}m)$) were measured by using two different active sensors (Greenseeker, NTech Inc. USA). The study was conducted in the years 2005-06 during the rice growing season at the experimental plots of National Institute of Agricultural Science and Technology located at Suwon, Korea. The experiments carried out with randomized complete block design with the application of four levels of nitrogen fertilizers (0, 70, 100, 130kg N/ha) and same amount of phosphorous and potassium content of the fertilizers. gNDVI and rNDVI increased as growth advanced and reached to maximum values at around early August, G(NDVI) were a decrease in values of observed with the crop maturation. gNDVI values and leaf nitrogen content were highly correlated at early July in 2005 and 2006. On the basis of this finding we attempted to estimate the leaf N contents using gNDVI data obtained in 2005 and 2006. The determination coefficients of the linear model by gNDVI in the years 2005 and 2006 were 0.88 and 0.94, respectively. The measured and estimated leaf N contents using gNDVI values showed good agreement ($R^2=0.86^{***}$). Results from this study show that gNDVI values represent a significant positive correlation with leaf N contents and can be used to estimate leaf N before the panicle formation stage. gNDVI appeared to be a very effective parameter to estimate leaf N content the rice canopy.

A Study on the Oxy-Combustion of the Coal in Drop Tube Furnace (Drop Tube Furnace에서 석탄의 순산소 연소 특성)

  • Roh, Seon Ah;Yun, Jin Han;Lee, Jung Kyu;Keel, Sang In;Min, Tai Jin;Kim, Sang-Bok;Park, In-Yong;Han, Bangwoo;Kim, Jin-Tae
    • Clean Technology
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    • v.27 no.4
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    • pp.367-371
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    • 2021
  • The oxy-combustion system is one of the carbon recovery and storage technologies (CCS: Carbon capture & storage) that performs coal combustion using pure oxygen and recirculated flue gas. This is a technology that facilitates storage of carbon dioxide by generating an exhaust gas consisting of only carbon dioxide without a process of separating carbon dioxide and nitrogen when coal is burned using pure oxygen and recirculated flue gas mixture instead of a conventional air combustion system that produces carbon dioxide and nitrogen mixed exhaust gas. In this study, the characteristics of generated NO and SO2 as atmospheric pollutants during oxy-combustion were examined using O2/CO2 mixed simulation gas. The reaction temperature was varied from 900 ℃ to 1200 ℃ and oxygen partial pressure was varied from 30% to 50%. The results showed that NO and SO2 concentrations in flue gas increased as the oxygen concentration and the reaction temperature in the furnace increased. The partial pressure of CO2 in flue gas also increased as the oxygen concentration and the reaction temperature in the furnace increased. As a results of comparing NO production of 30% O2/CO2 oxy-combustion with air combustion, NO in flue gas increased with reaction temperature in both experiments and NO of oxy-combustion was 40 ~ 80 ppm lower than that of air combustion.

Efficacy of Listeria Innocua Reduction on Enoki Mushrooms by Utilization of an Air Sterilization Device (공기 살균 장치 적용 팽이버섯 재배사의 Listeria Innocua 저감 효과)

  • Lee, Hyun-Dong;Yu, Byeong-Kee;Seo, Da-Som;Kim, Se-Ri;Lee, Chan-Jung;Kwak, Kang-Su
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.210-215
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    • 2021
  • For sterilization of microorganisms of the Listeria genus contaminating enoki mushroom, pilot mushroom grower equipped with air sterilization devices were developed. Sterilization experiments were performed using physical and chemical treatments. Internal temperature and humidity were controlled, maintaining 6.62℃±0.30 in the upper shelves, 6.46℃±0.24 in the middle shelves, and 6.48℃±0.25 in the lower shelves. Humidities were 79.97%±4.42, 79.43%±4.06, and 79.94±4.30%, respectively, with a temperature setting of 6.5℃, and a relative humidity of 75%. A suitable enoki mushroom cultivation stage for air sterilizer application was during the growth stage, with temperature in the 6.5~8.5℃ range, and humidity of 70~80%. At these same internal conditions, the ozone concentration in the mushroom cultivator was found to be 160 ppb during ion-cluster generator operation. After physical sterilization, the Listeria innocua survival rate was 0.1 to 0.9% using ion cluster sterilization, and 9.3 to 10.6% using UV air sterilization. The Listeria innocua survival rates on different materials were 9.3~10.6% on the metal specimen, and 9.9~16.2% on the plastic wrapper. The survival rate was particularly high on the rough side of the plastic wrapper. Ion cluster air sterilization is a labor-saving and effective method for suppressing the occurrence of Listeria bacteria on mushroom growers walls and shelves. For the plastic wrapper, chemical sterilization is more effective than physical sterilization.

Comparison of Reflectance and Vegetation Index Changes by Type of UAV-Mounted Multi-Spectral Sensors (무인비행체 탑재 다중분광 센서별 반사율 및 식생지수 변화 비교)

  • Lee, Kyung-do;Ahn, Ho-yong;Ryu, Jae-hyun;So, Kyu-ho;Na, Sang-il
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.947-958
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    • 2021
  • This study was conducted to provide basic data for crop monitoring by comparing and analyzing changes in reflectance and vegetation index by sensor of multi-spectral sensors mounted on unmanned aerial vehicles. For four types of unmanned aerial vehicle-mounted multispectral sensors, such as RedEdge-MX, S110 NIR, Sequioa, and P4M, on September 14 and September 15, 2020, aerial images were taken, once in the morning and in the afternoon, a total of 4 times, and reflectance and vegetation index were calculated and compared. In the case of reflectance, the time-series coefficient of variation of all sensors showed an average value of about 10% or more, indicating that there is a limit to its use. The coefficient of variation of the vegetation index by sensor for the crop test group showed an average value of 1.2 to 3.6% in the crop experimental sites with high vitality due to thick vegetation, showing variability within 5%. However, this was a higher value than the coefficient of variation on a clear day, and it is estimated that the weather conditions such as clouds were different in the morning and afternoon during the experiment period. It is thought that it is necessary to establish and implement a UAV flight plan. As a result of comparing the NDVI between the multi-spectral sensors of the unmanned aerial vehicle, in this experiment, it is thought that the RedEdeg-MX sensor can be used together without special correction of the NDVI value even if several sensors of the same type are used in a stable light environment. RedEdge-MX, P4M, and Sequioa sensors showed a linear relationship with each other, but supplementary experiments are needed to evaluate joint utilization through off-set correction between vegetation indices.

Effect of Cooling in a Semi-closed Greenhouse at High Temperature on the Growth and Photosynthesis Characteristics in Paprika (고온기 반밀폐형온실 냉방이 파프리카 생육과 광합성 특성에 미치는 영향)

  • Kim, Eun Ji;Park, Kyoung Sub;Goo, Hei Woong;Park, Ga Eun;Myung, Dong Ju;Jeon, Yong Hwan;Na, Haeyeong
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.335-341
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    • 2021
  • In this study, experiments were conducted to investigate the effects of high- temperature stress on paprika in a semi-closed greenhouse where cooling is available and a normal plastic greenhouse. Paprika grown in a semi-closed greenhouse in which geothermal cooling is provided showed a significantly higher speed of photosynthesis than paprika grown in a 3-layer plastic greenhouse in which there is no cooling system. It suggests that the photosynthesis speed of paprika in a plastic house decreases owing to high temperature stress. Plant height increased by 13cm more in the semi-closed greenhouse, and the size of leaf showed similar growth speed until the 2nd week after transplanting, however, after 3 weeks, the semi-closed greenhouse showed a big difference by 47% compared with the plastic greenhouse. In terms of the fruit count, the semi-closed greenhouse had 10.6 fruits/plant and the plastic greenhouse had 4.6 fruits/plant, indicating that the semi-closed greenhouse had a higher number of fruits by 130% than the plastic greenhouse. The fruit weight also presented a difference between the semi-closed greenhouse and the plastic greenhouse by 46%, which is 566.7g/plant and 387g/plant, respectively. According to the above mentioned results, it was validated that when paprika is cultivated in a semi-closed greenhouse where a cooling system is applied, photosynthesis and growth were better than in the normal plastic greenhouse. Thus, if the hot summer season is overcome by applying the elemental technologies for the cooling system to the normal plastic greenhouse, farm income may increase through improvement in the yield and quality.