• Title/Summary/Keyword: experimental medium

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Development of Medium for Griseofulvin Production: Part I. Screening of Medium Constituents Using the Plackett-Burman Experimental Design

  • Venkata, Dasu-V.;Panda, T.;Chidambaram, M.
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.355-359
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    • 2002
  • The Plackett-Burman experimental design was employed to evaluate the relative importance of medium constituents of each medium for enhanced griseofulvin production by Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvum MTCC 2004. It was found that the medium constituents, sucrose, $K_2HPO_4,\;NaNO_3,\;and\;FeSO_4{\cdot}7H_2O$, significantly influenced the griseofulvin production by Penicillium griseofulvum MTCC 1898. In the case of Penicillium griseofulvum MTCC 2004, lactose, glucose, and $MnSO_4{\cdot}H_2O$ significantly influenced the griseofulvin production.

Experimental Measurement and Monte Carlo Simulation the Correction Factor for the Medium-Energy X-ray Free-air Ionization Chamber

  • Yu, Jili;Wu, Jinjie;Liao, Zhenyu;Zhou, Zhenjie
    • Journal of the Korean Physical Society
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    • v.73 no.10
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    • pp.1466-1472
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    • 2018
  • A key comparison has been made between the air-kerma standards of the National Institute of Metrology (NIM), China, and other Asia Pacific Metrology Programme (APMP) members in the medium-energy X-ray. This paper reviews the primary standard Free-air ionization chamber correction factor experimental method and Monte Carlo simulation method in the NIM. The experimental method and the Monte Carlo simulation method are adopted to obtain the correction factor for the medium-energy X-ray primary standard free-air ionization chamber at 100 kV, 135 kV, 180 kV, 250 kV four CCRI reference qualities. The correction factor has already been submitted to the APMP as key comparison data and the results are in good agreement with those obtained in previous studies. This study shows that the experimental method and the EGSnrc simulation method are usually used in the measurement of the correction factor. In particular, the application of the simulation methods is more common.

Effects of nursing record education focused on legal aspects at small and medium sized hospitals

  • Do, Taehee;Kim, Heejung
    • The Journal of Korean Academic Society of Nursing Education
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    • v.27 no.2
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    • pp.152-162
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    • 2021
  • Purpose: The purpose of this study was to examine the effect of nursing record education on the knowledge and performance of nursing record of nurses at small- and medium-sized hospitals. Methods: The participants were 62 nurses working in two small- and medium-sized hospitals. Thirty-two nurses comprised the experimental group, and 30 nurses comprised the control group. Nursing record education was provided for the experimental group. Data were analyzed by x2-test and t-test analysis using the IBM SPSS statistics 25.0 Program. Results: After education, the knowledge (t=2.43, p=.019), performance (t=2.19, p=.033) and behavior scores (t=2.42, p=.018) on nursing record were significantly higher in the experimental group than in the control group. Based on this result, nursing record education is an effective intervention to improve nurses' knowledge and performance in writing nursing records in small- and medium-sized hospitals. Conclusion: We suggest the development of a systematic and standardized education program on nursing record including its legal aspects, for nurses in small- and medium-sized hospitals. The results of this study can be used as basic data for developing a nursing record education program for small- and medium-sized hospitals.

Implementation and Experimental Evaluation of Bandwidth Allocation Scheme on PROFIBUS (PROFIBUS에서 대역폭 할당 기법 구현 및 실험적 평가)

  • Hong, Seung-Ho;Kim, Yu-Chul;Kim, Ji-Yong
    • Journal of Institute of Control, Robotics and Systems
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    • v.6 no.11
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    • pp.943-954
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    • 2000
  • Fieldbus is the lowest level industrial network in the communication hierarchy of factory automation and distributed process control systems. Data generated from field devices are largely divided into three categories : time-critical, periodic and time-available data. Because these data share one fieldbus medium, it needs a method that allocates these data to the bandwidth-limited fieldbus medium. This paper introduces an implementation method of bandwidth allocation scheme on PROFIBUS. In order to implement bandwidth allocation scheme on PROFIBUS, the following functions need to be supplemented on the FDL(Fieldbus Datalink Layer) protocol: (i) separation of medium bandwidth into periodic and non-periodic intervals, (ii) synchronization of node timers over a local link. In order to examine the validity of bandwidth allocation scheme on PROFIBUS, this paper develops an experimental model of a network system. The results obtained from the experimental model show that the bandwidth allocation scheme satisfies the performance requirement of time-critical, periodic and time-available data.

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Studies on inoculation test of experimental animal and isolation and identificaton of Mycobacterium in tubercle and Iymph node of tuberculin(PPD) test positive dairy cattle. (Tuberculin(PPD)양성 반응우에 나타난 결핵 결절 및 림프절의 시험동물 접종 및 균분리 동정에 관한 연구)

  • Sung, Moung-Sook;Kim, Sin;Kim, Sang-Youn;Son, Jae-Kweon
    • Korean Journal of Veterinary Service
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    • v.20 no.2
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    • pp.205-215
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    • 1997
  • In this study, homogenized mixture of tubercle and pulmonary lymph node showed up tuberculin(PPD) positive dairy cattle was inoculated in experimental animal, and was cultured on medium(Lowenstein Jensen medium, 3% Ogawa). The results obtained through the survey were sumerized as follows ; 1. In experimental animal, goat and rabbits were decreased body weight(25∼28%) and died in 90 days with severe pathogenicity. Rats are slight pathogenicity. 2. Goat, rabbits and rats showed up severe lesions In lung, rabbits was also lesions other organs (kidney, appendix, ileocecum, and I lymph node). 3. Mycobacterium was grown between 5 weeks and 8 weeks on Lowenstein Jensen medium and 3% Ogawa medium. 4. Biochemical test of Mycobacterium cultured on medium was that niacin, nitrate reduction, tween 80 hydrolysis and catalase test were negative, but that urease test was positive. Therefore it was Mycobacterium bovis (M. bovis).

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Effect of Feeding High Forage Diets with Supplemental Fat on Blood Metabolites, Rumen Fermentation and Dry Matter Digestibility in Dairy Cows

  • Abdullah, M.;Young, J.W.;Tyler, H.D.;Mohiuddin, G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.4
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    • pp.451-456
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    • 2000
  • Fifty mid-lactation Holstein cows were used in a six-week feeding trial to study effects of high-forage, and high-fat diets on blood constituents, rumen fermentation and dry matter digestibility. Cows were divided into 10 replicates, each consisting of five cows. Each cow was assigned to a control (diet 1) or one of the four experimental diets (high-forage (75%), high-fat (7.5%) (diet 2); high-forage. medium-fat (5.0%) (diet 3); medium forage (65%), high-fat (diet 4); medium-forage, medium-fat (diet 5)), or a control diet containing about 50% forage and 2% fat. All diets were isonitrogenous (17.7% crude protein). The forage mixture consisted of 20% alfalfa hay, 40% alfalfa haylage, and 40% corn silage. Supplemental fat included 80% rumen-protected fat and 20% yellow grease. A non-significant difference was observed in concentrations of blood glucose for cows on different experimental and control diets. Plasma nonesterified fatty acids (NEFA) were higher in cows consuming experimental diets than those consuming the control diet. However, differences in NEFA concentrations in the plasma of cows consuming diets with different forage and fat levels were not significant. Rumen pH, concentration of volatile fatty acids (VFA) in rumen contents, and dry matter digestibility of control and experimental diets, and diets with different levels of forage and supplemental fat did not differ significantly.

Optimization of Medium and Fermentation Conditions for Mass Production of Bacillus licheniformis SCD121067 by Statistical Experimental Design (Bacillus licheniformis SCD121067 균체 생산성 증가를 위한 통계적 생산배지 및 발효조건 최적화)

  • Jeong, Yoo-Min;Lee, Ju-Hee;Chung, Hea-Jong;Chun, Gie-Taek;Yun, Soon-Il;Jeong, Yong-Seob
    • KSBB Journal
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    • v.25 no.6
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    • pp.539-546
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    • 2010
  • In this work, mass production of Bacillus licheniformis SCD121067 through medium optimization by statistical experimental method was studied. First, galactose, yeast extract and potassium phosphate dibasic were selected as carbon, nitrogen and phosphate sources for mass production of B. licheniformis SCD121067 by using one factor at a time method. Second, according to the result of Plackett-Burman experimental design, key factors was yeast extract and $K_2HPO$. Finally, the response surface methodology was performed to obtain the optimum concentrations of two selected variables. The optimized medium composition consisted of 20 g/L galactose, 36 g/L yeast extract, 0.41 g/L $K_2HPO4$, 0.25 g/L $Na_2CO_3$, 0.4g/L $MgSO_4$ and 0.01g/L $CaCl_2$. Dry cell weight (15.4 g/L) by optimum production medium were increased 10 times, as compared to that determined with basic production medium (1.5 g/L). Fermentation conditions were examined for the mass production of B. licheniformis. The effect of temperature, agitation speed, pH and aeration rate on the mass production of B. licheniformis were also studied in a batch fermenter which was carried out in a 2.5 L bioreactor with a working volume of 1.5 L containing optimized production medium. As a result, dry cell weight of batch culture was 30.7 g/L at $42^{\circ}C$, 300 rpm, pH 8.0 and 2 vvm.

The Effects of MPPA (Mileages/Points to Purchase Amount) Ratio on Consumers' Preference (구매금액 대비 마일리지/포인트의 비율이 소비자 선호에 미치는 영향)

  • Park, Sang-June;Byun, Ji-Yeon
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2008.10a
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    • pp.179-190
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    • 2008
  • Consumers earn a number of points for every purchase and then they can exchange a specified number of points for a desired reward in a typical loyalty program. The immediate payoff of their effort given as points is not the real reward they actually care about. It is merely an instrument (or medium) which has no value in itself. In a real world, consumers frequently choose the option with a bigger medium even though the economic value of the option is not changed by the medium. We call it 'medium effect'. In this study we explored if the size of medium affects on consumers' preferences. For this we controlled the reward options with three types of medium (small, medium, big) and measured the magnitude of preference difference among the three types of reward options. In addition, we manipulated comparability of reward options with wine and gas discount coupon. We confirmed that choosing one of two wines was easier than that of the two gas coupons. 164 respondents were allocated into three experimental groups and one control group. In three experimental groups, the ratios of the focused reward option's medium to the compared reward option's medium were different. For example, the focused reward option has 10 million points whereas the compared reward option has 10 million points for 1 million won purchase amount in the first group. Then each respondent was asked to choose one of two loyalty programs (focused program vs. compared program) in two different conditions (comparability between reward options: easiness vs. difficulty). To compare the medium effects among the experiment conditions we used chi-squares tests. The empirical results show the medium effect increases and then decreases as the ratio of reward mileages/points to purchase amount increases. Additionally, they let us know that comparability of alternatives affects on the medium effects depending on the ratio of reward mileages/points to purchase amount.

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The Effects of MPPA(Mileages/Points to Purchase Amount) Ratio on Consumer Preference (구매금액 대비 마일리지/포인트의 비율이 소비자 선호에 미치는 영향)

  • Park, Sang-June;Byun, Ji-Yeon
    • Journal of the Korean Operations Research and Management Science Society
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    • v.34 no.1
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    • pp.1-10
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    • 2009
  • Consumers earn a number of points for every purchase and then they can exchange a specified number of points for a desired reward in a typical loyalty program. The immediate payoff of their effort given as points is not the real reward they actually care about. It is merely an instrument (or medium) which has no value in itself. In a real world, consumers frequently choose the option with a bigger medium even though the economic value of the option is not changed by the medium. We call it 'medium effect.' In this study we explored if the size of medium affects consumers' preferences. For this we controlled the reward options with three types of medium (small, medium, big) and measured the magnitude of preference difference among the three types of reward options. In addition, we manipulated comparability of reward options with wine and gas discount coupon. We confirmed that choosing one of two wines was easier than that of the two gas coupons. 123 respondents were allocated into three experimental groups. In three experimental grounds, the ratios of the focused reward option's medium to the compared reward option's medium were different. For example, the focused reward option has 10 million points whereas the compared reward option has 10 million points for 1 million won purchase amount in the first group. Then each respondent was asked to choose one of two loyalty programs (focused program vs. compared program) in two different conditions (comparability between reward options. easiness vs. difficulty). To compare the medium effects among the experiment conditions we used chi-squares tests. The empirical results show consumer preference increases and then decreases as reward mile-ages/points given according to purchase amount increase. Additionally, they let us know that comparability of alter natives affects change of consumer preference by reward mileages/points.

Studies on Egg Production Index in Some Newly Evolved Multivoltine Breeds of the Silkworm, Bombyx mori L.

  • Gangopadhyay, Debnirmalya;Singh, Ravindra;Premalatha, V.;Rao, D.Raghavendra;Kariappa, B.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.7 no.2
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    • pp.117-125
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    • 2003
  • To know the impact of female pupal weight corresponding to the male cocoon shell weight upon fecundity, hatchability and percentage of non-viable eggs, the experimental multivoltine breeds namely BL67 and 96A were categorised into low, medium and high batches according to the weight of female pupae and male cocoon shells and moths emerged from low, medium and high female pupae were allowed to mate withmoths emerged from low, medium and high male cocoon shells. Both the experimental breeds of BL67 and 96A revealed a fairl trend in fecundity for high batch > medium batch > low batch. When data were statistically analysed, a significant (p < 0.05) and a highly significant (p < 0.01) increase in fecundity was observed in the medium and high batches of BL67 during November - December, 2002 whereas highly significant (p < 0.01) increase was observed in the high batches of 96A during November - December, 2002. Data revealed a highly significant (p < 0.01) increase in fecundity in medium and high batches of both the breeds during January - February, 2003. More interestingly, a significant (p < 0.05) reduction was noted in percentage of non-viable eggs in high batches of 96A during January - February, 2003. But no significant variation in hatchability was noticed due to the effect of differential female pupal weight and male cocoon shell weight.