Korean Journal of Veterinary Service (한국동물위생학회지)
- Volume 20 Issue 2
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- Pages.205-215
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- 1997
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- 3022-7372(pISSN)
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- 2287-7630(eISSN)
Studies on inoculation test of experimental animal and isolation and identificaton of Mycobacterium in tubercle and Iymph node of tuberculin(PPD) test positive dairy cattle.
Tuberculin(PPD)양성 반응우에 나타난 결핵 결절 및 림프절의 시험동물 접종 및 균분리 동정에 관한 연구
- Sung, Moung-Sook (Northern Branch of Kyungbuk Veterinary Service Laboratory) ;
- Kim, Sin (Northern Branch of Kyungbuk Veterinary Service Laboratory) ;
- Kim, Sang-Youn (Northern Branch of Kyungbuk Veterinary Service Laboratory) ;
- Son, Jae-Kweon (Northern Branch of Kyungbuk Veterinary Service Laboratory)
- 성명숙 (경상북도 가축위생시험소 북부지소) ;
- 김신 (경상북도 가축위생시험소 북부지소) ;
- 김상윤 (경상북도 가축위생시험소 북부지소) ;
- 손재권 (경상북도 가축위생시험소 북부지소)
- Published : 1997.05.01
Abstract
In this study, homogenized mixture of tubercle and pulmonary lymph node showed up tuberculin(PPD) positive dairy cattle was inoculated in experimental animal, and was cultured on medium(Lowenstein Jensen medium, 3% Ogawa). The results obtained through the survey were sumerized as follows ; 1. In experimental animal, goat and rabbits were decreased body weight(25∼28%) and died in 90 days with severe pathogenicity. Rats are slight pathogenicity. 2. Goat, rabbits and rats showed up severe lesions In lung, rabbits was also lesions other organs (kidney, appendix, ileocecum, and I lymph node). 3. Mycobacterium was grown between 5 weeks and 8 weeks on Lowenstein Jensen medium and 3% Ogawa medium. 4. Biochemical test of Mycobacterium cultured on medium was that niacin, nitrate reduction, tween 80 hydrolysis and catalase test were negative, but that urease test was positive. Therefore it was Mycobacterium bovis (M. bovis).