• Title/Summary/Keyword: evolutionary biology

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Ultrastructure of the Eye in the Atypus coreanus Kim, 1985 (한국땅거미(Atypus coreanus Kim, 1985)의 시각기 미세구조)

  • Kwon, Jung-Kyun;Ko, Myung-Kyoo;Chung, Ho-Sam;Kim, Joo-Pil
    • Applied Microscopy
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    • v.28 no.4
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    • pp.477-490
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    • 1998
  • Most spiders have four pairs of simple eyes. A few families of the spider have their developed eyes to an extent visual cues make up a significant of information to response from the external stimuli. Most spiders respond to the external stimuli around them. Specially, they are very sensitive to vibrations from the air, the ground, their webs, or even the surface of water. The present study was undertaken to examine the evolutionary development and function of eye with the observation of visual ultrastructure of Atypus coreanus Kim, 1985 using the electron microscopy. They have weak mobility, limited territory and low developed eyes. Atypus coreanus was collected from Mt. Ungil, Namyangju-gun, Kyonggi province. The fine structure of these eyes was examined by electron microscopy, and prepard by teasing method for scanning electron microscopic observation. As a result, the eyes of Atypus coreanus was composed of cornea, lens, vitreous body, retina, and rhabdome.

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Phylogenetic Relationships of Yeast-like Fungi Deduced from Partial Sequences of 18S Ribosomal RNA (18S 리보좀 RNA 부분 염기서열 분석에 의한 효모성 균류의 분자계통학적 연구)

  • Jung, Soo-Jin;Shin, Yong-Kook;Joo, Woo-Hong;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.23 no.4 s.75
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    • pp.310-317
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    • 1995
  • The evolutionary relationships for 43 species in the taxa of the basidiomycetous yeast and related yeast-like fungi have been studied from the 43 18S rRNA partial sequences. Fibulobasidium inconspicuum showed a relatively close relationship to Filobasidiella neoformans. It is obvious that Tremella foliacea and Ustilogo rabenhorstiana form a independently different cluster from basidiomycetous yeast and seem to be phylogenetically distant from basidiomycetous yeast and each other. The basidiomycetous yeasts are believed more closely associated with Tremella foliacea than with Ustilago rabenhorstiana. The phenotypic criteria such as carotenoid pigments, teliospores, ballistospores seems not to be phylogenetically useful indicators.

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Monitoring Benthic AIgal Communides:A Comparison of Targeted and Coefficient Sampling Methods

  • Edwards, Matthew S.;Tinker, Martin T.
    • ALGAE
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    • v.24 no.2
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    • pp.111-120
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    • 2009
  • Choosing an appropriate sample unit is a fundamental decision in the design of ecological studies. While numer-ous methods have been developed to estimate organism abundance, they differ in cost, accuracy and precision.Using both field data and computer simulation modeling, we evaluated the costs and benefits associated with twomethods commonly used to sample benthic organisms in temperatc kelp forests. One of these methods, theTargeted Sampling method, relies on different sample units, each "targeted" for a specific species or group ofspecies while the other method relies on coefficients that represent ranges of bottom cover obtained from visual esti-mates within standardized sample units. Both the field data and the computer simulations suggest that both meth-ods yield remarkably similar estimates of organisnm abundance and among-site variability, although the Coefficientmethod slightly underestimates variability armong sample units when abundances are low. In contrast, the twomethods differ considerably in the effort needed to sample these communities; the Targeted Sampling requiresmore time and twice the persormel to complete. We conclude that the Coeffident Sampling metliod may be bettcrfor environmental monitoring programs where changes in mean abundance are of central conccm and resources arelimiting, but that the Targeted sampling methods may be better for ecological studies where quantitative reIation-ships among species and small-scale variability in abundance are of central concern.

Growth and nutrient bioextraction of Gracilaria chorda, G. vermiculophylla, Ulva prolifera, and U. compressa under hypo- and hyper-osmotic conditions

  • Wu, Hailong;Shin, Sook Kyung;Jang, Sojin;Yarish, Charles;Kim, JangKyun
    • ALGAE
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    • v.33 no.4
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    • pp.329-340
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    • 2018
  • The present study was to determine the effects of salinity on the growth and nutrient bioextraction abilities of Gracilaria and Ulva species, and to determine if these seaweeds can be used for nutrient bioextraction under hypo- and / or hyperosmotic conditions. Two Gracilaria species, G. chorda and G. vermiculophylla, and two Ulva species, U. prolifera and U. compressa, were cultured at various salinity conditions (5, 10, 15, 20, 30, 40, and 50 psu) for 3 weeks. Results showed that the growth rates, nutrient uptake, tissue nutrient contents and nutrient removal were significantly affected by salinity and species. All four species were euryhaline with the highest growth rates at 20 psu. Among the four species, U. prolifera, U. compressa, and G. vermiculophylla showed potential to be used for nutrient bioextraction in estuaries and / or land-based fish farms due to their rapid growth, high nutrient uptake, high tissue carbon and nitrogen accumulation and removal capacities.

Identification and Phylogenetic Analysis of Long Terminal Repeat Elements of the Human Endogenous Retrovirus K Family (HERV-K) from a Human Brain cDNA Library

  • Kim, Heui-Soo;Lee, Young-Choon
    • Animal cells and systems
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    • v.5 no.2
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    • pp.133-137
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    • 2001
  • Long terminal repeats (LTRs) of the human endogenous retrovirus K family (HERV-K) have been found to be coexpressed with sequences of genes closely located nearby. We examined transcribed HERV-K LTR elements in human brain tissue. Using cDNA synthesized from mRNA of the human brain, we performed PCR amplification and identified ten HERV-K LTR elements. These LTR elements showed a high degree of sequence similarity (92.4-99.7%) with the human-specific LTR elements. A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-K LTR elements could be divided into two groups through evolutionary divergence. Some HERV-K LTR elements (HKL-B7, HKL-B8, HKL-B10) belonging to the group II from human brain cDNA were closely related to the human-specific HERV-K LTR elements. Our data suggest that HERV-K LTR element are active in the human brain; they could conceivably play a pathogenic role in human diseases such as psychosis.

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HCoV-IMDB: Database for the Analysis of Interactions between HCoV and Host Immune Proteins

  • Kim, Mi-Ran;Lee, Ji-Hae;Son, Hyeon Seok;Kim, Hayeon
    • International journal of advanced smart convergence
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    • v.8 no.1
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    • pp.1-8
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    • 2019
  • Coronaviruses are known respiratory pathogens. In the past, most human coronaviruses were thought to cause mild symptoms such as cold. However recently, as seen in the Severe Acute Respiratory Syndrome (SARS) and the Middle East Respiratory Syndrome (MERS), infectious diseases with severe pulmonary disease and respiratory symptoms are caused by coronaviruses, making research on coronaviruses become important. Considering previous studies, we constructed 'HCoV-IMDB (Human Corona Virus Immune Database)' to systematically provide genetic information on human coronavirus and host immune information, which can be used to analyze the interaction between human coronavirus and host immune proteins. The 'HCoV-IMDB' constructed in the study can be used to search for genetic information on human coronavirus and host immune protein and to download data. A BLAST search specific to the human coronavirus, one of the database functions, can be used to infer genetic information and evolutionary relationship about the query sequence.

The complete chloroplast genome of Diarthron linifolium (Thymelaeaceae), a species found on a limestone outcrop in eastern Asia

  • KIM, Sang-Tae;OH, Sang-Hun;PARK, Jongsun
    • Korean Journal of Plant Taxonomy
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    • v.51 no.4
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    • pp.345-352
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    • 2021
  • Diarthron linifolium Turcz. is an annual herb usually found in sandy soil or limestone areas. Plants in the genus Diarthron are known to have toxic chemicals that may, however, be potentially useful as an anticancer treatment. Diarthron linifolium is a unique species among the species of the genus distributed in Korea. Here, we determine the genetic variation of D. linifolium collected in Korea with a full chloroplast genome and investigate its evolutionary status by means of a phylogenetic analysis. The chloroplast genome of Korean D. linifolium has a total length of 172,644 bp with four subregions; 86,158 bp of large single copy and 2,858 bp of small single copy (SSC) regions are separated by 41,814 bp of inverted repeat (IR) regions. We found that the SSC region of D. linifolium is considerably short but that IRs are relatively long in comparison with other chloroplast genomes. Various simple sequence repeats were identified, and our nucleotide diversity analysis suggested potential marker regions near ndhF. The phylogenetic analysis indicated that D. linifolium from Korea is a sister to the group of Daphne species.

Endobiotic microalgae in molluscan life

  • Sokolnikova, Yulia
    • Fisheries and Aquatic Sciences
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    • v.25 no.10
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    • pp.499-516
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    • 2022
  • Endobiotic microalgae inhabit various groups of organisms, including bivalves. In this group, the association between the giant molluscs Tridacna and Symbiodinium is one of the most recognizable. This consortium allows hydrobionts to survive in oligotrophic waters by regulating their metabolism. The available research has provided an understanding of the interaction and adaptation of these symbionts, but the problem of the beginning of the formation of these relationships remains unresolved. In the case of Tridacninae, symbiosis is essential for the survival of bivalves, in contrast to representatives of the Mytilidae and the Coccomyxa found in them. A few works devoted mainly to the morphological aspects of invasion have shown that endobiont causes inflammation and pathology. Having data to clarify the exact "diagnosis" of the interaction of these organisms is not enough. It is possible that the relationship between bivalves and Coccomyxa is in the early stages of being established, which may lead to mutualism or parasitism in the future. We assume that the analysis of works on the symbiosis of Symbiodinium and bivalves will facilitate the course of research for the less studied Coccomyxa and their hosts. By postulating the Coccomyxa represent a unique evolutionary model for the formation of a symbiotic system, it is possible to use this system to study the interaction of organisms during their initial contact. The identified signalling pathways and mechanisms that allow the photobionts to evade host immunity can be useful for constructing new forms of symbiosystems.

Kelps in Korea: from population structure to aquaculture to potential carbon sequestration

  • Hwang, Eun Kyoung;Boo, Ga Hun;Graf, Louis;Yarish, Charles;Yoon, Hwan Su;Kim, Jang Kyun
    • ALGAE
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    • v.37 no.2
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    • pp.85-103
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    • 2022
  • Korea is one of the most advanced countries in kelp aquaculture. The brown algae, Undaria pinnatifida and Saccharina japonica are major aquaculture species and have been principally utilized for human food and abalone feed in Korea. This review discusses the diversity, population structure and genomics of kelps. In addition, we have introduced new cultivar development efforts considering climate change, and potential carbon sequestration of kelp aquaculture in Korea. U. pinnatifida showed high diversity within the natural populations but reduced genetic diversity in cultivars. However, very few studies of S. japonica have been conducted in terms of population structure. Since studies on cultivar development began in early 2000s, five U. pinnatifida and one S. japonica varieties have been registered to the International Union for the Protection of New Varieties of Plants (UPOV). To meet the demands for seaweed biomass in various industries, more cultivars should be developed with specific traits to meet application demands. Additionally, cultivation technologies should be diversified, such as integrated multi-trophic aquaculture (IMTA) and offshore aquaculture, to achieve environmental and economic sustainability. These kelps are anticipated to be important sources of blue carbon in Korea.

Cytosolic domain regulates the calcium sensitivity and surface expression of BEST1 channels in the HEK293 cells

  • Kwon Woo Kim;Junmo Hwang;Dong-Hyun Kim;Hyungju Park;Hyun-Ho Lim
    • BMB Reports
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    • v.56 no.3
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    • pp.172-177
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    • 2023
  • BEST family is a class of Ca2+-activated Cl- channels evolutionary well conserved from bacteria to human. The human BEST paralogs (BEST1-BEST4) share significant amino acid sequence homology in the N-terminal region, which forms the transmembrane helicases and contains the direct calcium-binding site, Ca2+-clasp. But the cytosolic C-terminal region is less conserved in the paralogs. Interestingly, this domain-specific sequence conservation is also found in the BEST1 orthologs. However, the functional role of the C-terminal region in the BEST channels is still poorly understood. Thus, we aimed to understand the functional role of the C-terminal region in the human and mouse BEST1 channels by using electrophysiological recordings. We found that the calcium-dependent activation of BEST1 channels can be modulated by the C-terminal region. The C-terminal deletion hBEST1 reduced the Ca2+-dependent current activation and the hBEST1-mBEST1 chimera showed a significantly reduced calcium sensitivity to hBEST1 in the HEK293 cells. And the C-terminal domain could regulate cellular expression and plasma membrane targeting of BEST1 channels. Our results can provide a basis for understanding the C-terminal roles in the structure-function of BEST family proteins.