• BMB Reports
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• v.35 no.5
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• pp.472-475
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• 2002

Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lectins that possess canonical dimers, thermal denaturation studies show that the refolding of phytohemagglutinin L at neutral pH is irreversible. Differential scanning calorimetry (DSC) was used to study the denaturation of this lectin as a function of pH that ranged from 2.0 to 3.0. The lectin was found to be extremely thermostable with a transition temperature around $82^{\circ}C$ and above $100^{\circ}C$ at pH 2.5 and 7.2, respectively. The ratio of calorimetric to vant Hoff enthalpy could not be calculated because of its irreversible-folding behavior. However, from the DSC data, it was discovered that the protein remains in its compact-folded state, even at pH 2.3, with the onset of denaturation occurring at $60^{\circ}C$.

• Korean Institute of Interior Design Journal
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• v.20 no.1
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• pp.14-23
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• 2011

The purpose of this study is to analyse the inclinations and expressions in contemporary architecture. Specially, we call this tendency and architectural movements as architectural metamorphosis. Metamorphosis in architecture present the core of the change of Forms and spirits in a change of outward shape and terrestrial identity. As in Ovid's extended dramatic poem of change and transformation, Metamorphoses, all Souls are deathless, and migrates from one form to another. Like these stories in Metamorphoses, Ovid tells the soul never dies, but leaps one form to anther, and can take any shape. So the architectural form, transformation and deformation in contemporary architecture means architectural sensations and cognitions can even approach the soul of form and shape under the transformation. The expressions and design strategies of metamorphosis in comtemporary architecture reveal continuous and sequential formations of space, linear structure with force and vector, rhythmical wavement and folding surface, lively wiggly flows of volumns and objects, and so on. Such qualities came from the periodical needs; separation of structure and surface, poly-surfacial movement, poly-sensual expression and experience, dematerialization and the dematerialized space, formless of non-formal architecture, digital architecture. Architecture of Metamorphosis is the ways and the needs of our period to overcome the static limits prohibits the liberal thoughts, to find the ways toward the opportunities and diversities and to unlock the imaginaire of the contemporary architecture.

• Journal of the Korea Furniture Society
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• v.25 no.2
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• pp.139-147
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• 2014

This paper researched the woodcraft in the Baekje kingdom since the study on the Baekje furniture has not been established due to the absence of the artifacts or remains related to the furniture in the Baekje period. Accordingly, the study focused on the excavated Baekje wooden products and wood crafts on the basis of ancient documents and investigated the ancient documents supposedly related to the Baekje furniture: The Chronicles of the Three States and Japan Scribe. As the result of the study, it could be summarized as follows. First of all, the woodcraft materials were wood, whagak, ivory, silver, gold and they were domestic or imported. Secondly, the woodcraft was finished naturally or lacquered with black or red color or varnishing with lacquer. Thirdly, the kinds of furniture used in the Baekje were a desk, a small portable dinning table, a table and folding stool. Even though there has been no heritage of the Baekje furniture and woodcraft and it is difficult to explain the characteristics or give an example of the Baekje furniture in detail, this study seems to be significant now that it researched the documents and related artifacts or remains which could give some hints or hypothesis of the furniture and woodcraft in the Baekje. What is more, there have been no sound studies on the Baekje furniture.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.11
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• pp.1662-1667
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• 2012

This paper describes the design of size-reduced ring hybrid couplers (RHC) using a common defected ground structure (CDGS). The proposed RHC consists of double-sided microstrip line and the CDGS patterns which are placed in the common ground plane of the double-sided microstrip line. The effects of DGS as a periodic structure are imposed on both microstrip lines, so the advantages of the existing DGS are doubled. The normal RHC is designed first and folded by the half reference plane with the CDGS realized on the common ground plane. So another trial for size-reduction is performed by folding the RHC and inserting CDGS besides to the conventional DGS. In order to show a design example, a 2GHz RHC is designed and miniaturized using CDGS. The size of the miniaturized RHS is around 50% of the normal one, while the performances are well preserved even after the size-reduction.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.1
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• pp.97-105
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• 2015

Recently, there have been many researches about applications of origami to mechanical engineering, which realizes a 30 sturcture by folding a 20 plane material. With this simple manufacturing process, origami was even adopted by some roboticists as a way to build an entirely new robot with benefits in terms of cost, weight, and structural simplicity In this paper, we propose a new type of a walking robot based on origami structure. Because all the components of the robot that generate gait motion are mechanically connected, it can actually walk fotward with only a single actuator. We also showed the similarity of gait trajectories between a kinematic analysis and the actual gait motion measured by video tracking. This result proved the possibility of designing an origami-based robot with the identical gait trajectory as we plan.

• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.72-77
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• 2009

Here, we demonstrate that the overexpression of the GroELS chaperone system, which assists the folding of intracellular proteins and prevents aggregation of its biological targets, can enhance the thermotolerance of Escherichia coli strains and facilitate the production of recombinant protein under thermal stress. The overexpression of GroELS led to an about 2-fold higher growth rate of E. coli XL-1 blue than control at $45^{\circ}C$ and induced the growth of the strain even at $50^{\circ}C$, although the growth was not sustained in the second-round culture. The effect of GroELS overexpression was also effective on other E. coli strains such as JM109, $DH5{\alpha}$, and BL21. Finally, we have shown that coexpression of GroELS allows us to produce recombinant protein even at $50^{\circ}C$, a temperature at which the protein production based on E. coli is not efficient. This study indicates that the employment of the GroELS overexpression system can expand the range of environmental conditions for E. coli.

• Journal of Elementary Mathematics Education in Korea
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• v.20 no.4
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• pp.695-715
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• 2016

This study is on the teaching method for the students who belong to the same school (one, the gifted class, passed gifted education of Science High school ), 1-1, face-to-face learning (two, good students in regular classroom) with a teacher, paired learning teams (4 people, gifted classes), and group lessons (20 people, gifted classes) and using the justification analysis framework tool(PIRSO) of Kim(2010) analyzes the justification element of the students in the group classes regular polygons paper was to explore ways to improve the justification of the folding maps activities. As a result, the width of the largest polygon difficulty level appropriate to the class for gifted elementary school classes but the individual learning style of the 1-1 face-to-face with a teacher or discussion with colleagues and cooperative approach is justified, rather than the material of the study of origami activities it turned out to be more effective in improving the level of justification. Unlike the individual learning activities, the exploration for class is the need to strain in parallel to the student is selected as needed, rather than serial manner was confirmed that it is necessary to clearly present problems even from the beginning. Development of teaching through the implications obtained from this method of reconstruction activities and proposed improvement measures for questioning.

• Journal of Bio-Environment Control
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• v.11 no.1
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• pp.5-11
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• 2002

In this study, new development of natural ventilation window was accomplished to control environment of greenhouse with no use of farced ventilation during hot season. The ventilation effect of developed ventilation window was investigated in experimental greenhouse which was designed using side wall panel and folding type panel fur natural ventilation. Folding panel type ventilation window was designed to open upper part of the side wall and top of the roof using two hinges which are located bottom of the side wall and the roof panel to grab one side of each panels and guide the other side along with the guidance rail. Developed ventilation window has top ventilation part with maximum moving distance X=ι (1-cos$\theta$)=848.5 mm and side ventilation part with maximum moving distance Y=ι/2 $\times$sin$\theta$=1,184.4 mm at 45$^{\circ}$ of theoretical opening angle. It took 4.5 minutes to open roof vent fully and temperature at 1.2 and 0.8 m height decreased after 1 minute from starting opening and became equilibrium state maintaining 3-4$^{\circ}C$ difference after 2 minutes from complete opening. Air exchange rate was 15.2~39.3 h$^{-1}$ which was more than 10~15 h$^{-1}$ of continuous type and Venlo type greenhouse. The descent effect of temperature by ventilation windows was two times higher than Venlo type greenhouse.

• Ocean and Polar Research
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• v.25 no.1
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• pp.63-74
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• 2003

The build-up of the heat field in shallow coastal water due to a point source has been investigated using an analytical solution of a time-integral form derived by extending the solutions by Holley(1969) and also presented in Harleman (1971). The uniform water depth is assumed with non-isotropic turbulent dispersion. The alongshore-flow is assumed to be uni-directional, spatially uniform and oscillatory. Due to the presence of the oscillatory alongshore-flow, the heat build-up occurs in an oscillatory manner, and the excess temperature thereby fluctuates in that course and even in the quasi-steady state. A series of calculations reveal that proper choices of the decay coefficient as well as dispersion coefficients are critical to the reliable prediction of the excess temperature field. The dispersion coefficients determine the absolute values of the excess temperature and characterize the shoreline profile, particularly within the tidal excursion distance, while the decay coefficient determines the absolute value of the excess temperature and the convergence rate to that of the quasi-steady state. Within the e-folding time scale $1/k_d$ (where $k_d$ is the heat decay coefficient), heat build-up occurs more than 90% of the quasi-steady state values in a region within a tidal excursion distance (L), while occurs increasingly less the farther we go to the downstream direction (about 80% at 1.25L, and 70% at 1.5L). Calculations with onshore and offshore discharges indicate that thermal spreading in the direction of the shoreline is reduced as the shoreline constraint which controls the lateral mixing is reduced. The importance of collecting long-term records of in situ meteorological conditions and clarifying the definition of the heat loss coefficient is addressed. Interactive use of analytical and numerical modeling is recommended as a desirable way to obtain a reliable estimate of the far-field excess temperature along with extensive field measurements.

• The Korean Journal of Community Living Science
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• v.22 no.1
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• pp.53-61
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• 2011

We have made knitwear in a different way from the typical woven pattern; it does not have the dart, even for women's tops. The purpose of this research was to compare the fit and appearance of the torso pattern for circular knitted fabric between virtual try-on garments and real garments. First, a woman in her 20's was scanned by a 3D body scanner, and thus producing a virtual avatar. I prepared knit patterns and created a torso pattern appropriate for circular knitted fabric. Next, I measured the body size of the avatar. The 2D patterns for the torso and sleeves were developed using the Yuka Super ALPHA : Plus. The 3D virtual garments were made from the imported 2D patterns and were then tried on the 3D virtual avatars. Finally, the fit of the real garments and the virtual garments was compared. While the shape of the virtual try-on garments were similar to the real garments, the folding and sagging were different. This study found the length was the same as woven wear. However the actual bust size and the clothing size were the same which is not a typical characteristic of woven wear. In the case of hem measurement, more space was needed than actual body size but less space was needed than in woven patterns.