• Title/Summary/Keyword: evaluation of reaction accuracy

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Techniques for Evaluation of LAMP Amplicons and their Applications in Molecular Biology

  • Esmatabadi, Mohammad javad Dehghan;Bozorgmehr, Ali;zadeh, Hesam Motaleb;Bodaghabadi, Narges;Farhangi, Baharak;Babashah, Sadegh;Sadeghizadeh, Majid
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.17
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    • pp.7409-7414
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    • 2015
  • Loop-mediated isothermal amplification (LAMP) developed by Notomi et al. (2000) has made it possible to amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. The ultimate products of LAMP are stem-loop structures with several inverted repeats of the target sequence and cauliflower-like patterns with multiple loops shaped by annealing between every other inverted repeats of the amplified target in the similar strand. Because the amplification process in LAMP is achieved by using four to six distinct primers, it is expected to amplify the target region with high selectivity. However, evaluation of reaction accuracy or quantitative inspection make it necessary to append other procedures to scrutinize the amplified products. Hitherto, various techniques such as turbidity assessment in the reaction vessel, post-reaction agarose gel electrophoresis, use of intercalating fluorescent dyes, real-time turbidimetry, addition of cationic polymers to the reaction mixture, polyacrylamide gel-based microchambers, lateral flow dipsticks, fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays and nanoparticle-based colorimetric tests have been utilized for this purpose. In this paper, we reviewed the best-known techniques for evaluation of LAMP amplicons and their applications in molecular biology beside their advantages and deficiencies. Regarding the properties of each technique, the development of innovative prompt, cost-effective and precise molecular detection methods for application in the broad field of cancer research may be feasible.

User Evaluation of Encountered Type Haptic System with Visual-Haptic Co-location (시각 - 촉각 일치된 마중형 햅틱 제시 시스템의 사용자 평가)

  • Cha, Baekdong;Bae, Yoosung;Choi, Wonil;Ryu, Jeha
    • Journal of the HCI Society of Korea
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    • v.14 no.2
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    • pp.13-20
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    • 2019
  • For encountered haptic display systems among the virtual training systems for industrial safety, visual-haptic co-location is required for natural interaction between virtual and real objects. In this paper, we performed the user evaluation of the immersive VR haptic system which implement some level of visual-haptic co-location through a careful and accurate calibration method. The goal of the evaluation is to show that user performance (reaction time and distance accuracy) for both environments is not significantly different for certain tasks performed. The user evaluation results show statistically significant differences in reaction time but the absolute difference is less than 1 second. In the meantime, the distance accuracy shows no difference between the virtual and the actual environments. Therefore, it can be concluded that the developed haptic virtual training system can provide inexpensive industrial safety training in place of costly actual environment.

A Study on Classification Evaluation Prediction Model by Cluster for Accuracy Measurement of Unsupervised Learning Data (비지도학습 데이터의 정확성 측정을 위한 클러스터별 분류 평가 예측 모델에 대한 연구)

  • Jung, Se Hoon;Kim, Jong Chan;Kim, Cheeyong;You, Kang Soo;Sim, Chun Bo
    • Journal of Korea Multimedia Society
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    • v.21 no.7
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    • pp.779-786
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    • 2018
  • In this paper, we are applied a nerve network to allow for the reflection of data learning methods in their overall forms by using cluster data rather than data learning by the stages and then selected a nerve network model and analyzed its variables through learning by the cluster. The CkLR algorithm was proposed to analyze the reaction variables of clustering outcomes through an approach to the initialization of K-means clustering and build a model to assess the prediction rate of clustering and the accuracy rate of prediction in case of new data inputs. The performance evaluation results show that the accuracy rate of test data by the class was over 92%, which was the mean accuracy rate of the entire test data, thus confirming the advantages of a specialized structure found in the proposed learning nerve network by the class.

Parametric Study for Assessment of Reaction Forces on Ship Docking Supports

  • Ryu, Cheol-Ho;Kim, Sung-Chan;Lee, Jang-Hyun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.3
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    • pp.290-301
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    • 2013
  • The docking analysis of a global ship structure is requested to evaluate its structural safety against the reaction forces at supports during docking works inside a dry dock. That problem becomes more important recently as the size of ships is getting larger and larger. The docking supports are appropriately arranged in a dock to avoid their excessive reaction forces which primarily cause the structural damages in docking a ship and, up to now, the structural safety has been assessed against the support arrangement by the finite element analysis (FEA) of a global ship structure. However, it is complicated to establish the finite element model of the ship in the current structural design environment of a shipyard and it takes over a month to finish the work. This paper investigates a simple and fast approach to carry out a ship docking analysis by a simplified grillage model and to assign the docking supports position on the model. The grillage analysis was considered from the motivation that only the reaction forces at supports are sufficient to assess their arrangement. Since the simplified grillage model of the ship cannot guarantee its accuracy quantitatively, modeling strategies are proposed to improve the accuracy. In this paper, comparisons between the proposed approach and three-dimensional FEA for typical types of ships show that the results from the present grillage model have reasonably good agreement with the FEA model. Finally, an integrated program developed for docking supports planning and its evaluation by the proposed approach is briefly described.

Comparative Study of Text Entry Speed and Accuracy Using the Three Different Keyboard Type in Students with Cerebral Palsy: Case Study (키보드 유형에 따른 뇌성마비 학생의 문자입력 속도 및 정확도 비교: 사례연구)

  • Jeong, Dong-Hoon
    • Journal of the Korean Society of Physical Medicine
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    • v.10 no.1
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    • pp.23-35
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    • 2015
  • PURPOSE: People with physical disabilities such as cerebral palsy usually experience obstacles when interacting with computer through conventional keyboard because of their motor disabilities. The purpose of this study is empirically compare of text entry(alphabet and word) speed and accuracy using the three different keyboard type on four students(male 2 and female 2) with cerebral palsy. METHODS: This research design used a replicated single-case experimental approach to compare the individual performance. An alternating treatments design was used to examine the effectiveness of standard QWERTY keyboard and alternative keyboard(mini and big keyboard) on computer access for students with cerebral palsy. To avoid changes in posture that influence a keyboard character entry training and evaluation was carried out using his sitting in a wheelchair. Compass software program used in this study as an assessment tool to measure speed and accuracy when performance of text entry(alphabet and word). This was repeated until the stable status of reaction time. RESULTS: As a result, the alternative keyboard seems to be the most effective device for students with cerebral palsy to perform text entry. But various factors such as peculiarity of motor disabilities, experience and preferences of the user are heavily related. CONCLUSION: Thus, we must perform the objective and systematic assessment for computer access and if sustained training is accomplished, it could to improve speed and accuracy of text entry(alphabet and word).

Icon Evaluation Using Spreading Activation Model (활성화 확산 모형을 이용한 아이콘 평가)

  • Lee, Sun-Jung;Myung, Ro-Hae
    • Journal of the Ergonomics Society of Korea
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    • v.26 no.4
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    • pp.135-142
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    • 2007
  • Icon conveys symbolic information which they have become ubiquitous as main element of GUI. However, users often cannot intuitively understand its functional meanings. Icon has to be designed for users to easily understand functional meanings. In order to evaluate icon, the spreading activation model can be used to effectively understand the process of information retrieval. In asymmetric spreading activation model is that in the two nodes the degree of spreading activation is different according to direction. Thus, asymmetric spreading activation theory was performed evaluating the strength of association when users see visual image to associate their verbal meaning (visual image - verbal meaning pair) and users see verbal meaning to associate their visual image (verbal meaning - visual image pair). According to the direction, this study hypothesizes that the well-designed icons have symmetric relationship rather than asymmetric relationship between the two pairs. The strength of association is measured through the reaction time and the accuracy rate. In performing SAT (spreading activation test), the ten icons were selected as word processing software icons. After first SAT, newly designed icons were developed based on Korean mental model, and second SAT was conducted using them. The results showed that the accuracy rate of newly designed icon has been improved. Also there is significant difference of reaction time between current icons and newly designed icons. Well-designed icon is confirmed that the strength of association relationship arises symmetrically rather than asymmetrically between the two pairs. User centered icons could be designed by improving the strength of association between the two pairs. Asymmetric SAT evaluates the strength of the association between the visual image and the verbal meanings to contribute to the development of icon which it related to human's association structure.

A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation

  • Kang, Myung Joo;Cho, Ha Ra;Lee, Dong Hoon;Yeom, Dong Woo;Choi, Young Wook;Choi, Yong Seok
    • Mass Spectrometry Letters
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    • v.5 no.3
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    • pp.79-83
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    • 2014
  • Three different dutasteride extraction methods were compared and a method based on liquid-liquid extraction (LLE) using methyl tert-butyl ether and methylene chloride was proved to be more effective than others for the extraction of dutasteride and finasteride, the internal standard (IS), from rat plasma. Additionally, a method composed of the LLE extraction, liquid chromatography, and multiple reaction monitoring (MRM) to target dutasteride and IS was validated by assessing specificity, linearity ($r^2$ = 0.9993, 5 - 400 ng/mL), sensitivity (the limit of detection: 4.03 ng/mL; the limit of quantitation: 12.10 ng/mL), accuracy (intra-day: 89.4 - 105.9%; inter-day: 84.9 - 100.9%), precision (intra-day: 0.8 - 6.9%; inter-day: 2.9 - 15.9%), and recovery (84.7 - 107.8%). Since the validated method was successfully applied to a pharmacokinetic study of dutasteride, it can be useful for the pharmacokinetic evaluation of newly developed dutasteride formulations.

Development and Validation of Robot Steered EPS HILS System (로봇 조향 기반 EPS HILS 시스템의 개발 및 검증)

  • Hong, Taewook;Kwon, Jaejoon;Park, Kihong;Ki, Siwoo;Choi, Sangsoo
    • Journal of the Korean Society for Precision Engineering
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    • v.30 no.1
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    • pp.85-95
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    • 2013
  • As the conventional hydraulic power steering system in the passenger vehicles is being rapidly replaced by EPS (Electric Power Steering) system, performance evaluation of the EPS system has become an important issue in the automotive industries. But the evaluation process takes significant expertise since steering conditions in the test protocols must be implemented with high accuracy. EPS HILS (Hardware-In the-Loop Simulation) system is developed together with robot steering system in this study. Main components of EPS HILS system include: C-EPS hardware, CarSim vehicle model, and road reaction force generation system powered by servo motor. The robot steering system, operated by another servo motor, was combined with EPS HILS system to substitute for steering efforts of human driver. The road reaction force generation system and the robot steering system were carefully validated by using the data obtained from vehicle tests. An on-center handling test was conducted by using EPS HILS system combined with the robot steering system. In the result of this study, robot-steered EPS HILS system developed with its high reliability and no need of skilled driver's, can be widely adopted to evaluate any performance of EPS system.

Analysis of dutasteride in human serum by LC-MS/MS (LC-MS/MS를 이용한 혈청 중 dutasteride 분석)

  • Nam, Hye-Seon;Nam, Kyong-Hee;Jung, Su-Hee;Lee, Jang-Woo;Kang, Jin-Yeong;Hong, Soon-Keun;Kim, Tae-Sung;Jung, Ki-Kyung;Kang, Tae-Seok;Yoon, Hae-Jung;Lee, Kwang-Ho;Rhee, Gyu-Seek
    • Analytical Science and Technology
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    • v.25 no.1
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    • pp.76-82
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    • 2012
  • The determination and confirmation of dutasteride in human serum was performed by a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Beclomethasone as an internal standard (I.S.) was added to the serum and the mixed sample was pretreated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE). The mass transitions of dutasteride and I.S. monitored in multiple reaction monitoring (MRM) were m/z 529.6${\rightarrow}$461.5 and m/z 409.3${\rightarrow}$391.2, respectively, and the retention times were 6.45 and 5.46 min, respectively. The calibration curve was linear in the concentration range of 0.5~30.0 ng/mL ($R^2$= 0.9999) and the limit of quantitation (LOQ) was found to be 0.5 ng/mL. The recovery of dutasteride was shown to be 66~72%. The intra-day assay precision and accuracy were in the range 3.5~5.5% and 85.7~89.9%, respectively, and the interday assay precision and accuracy were in the range 4.2~5.8% and 90.8~95.8%, respectively.

Simultaneous Quantitative Determination of Nine Hallucinogenic NBOMe Derivatives in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry

  • Seo, Hyewon;Yoo, Hye Hyun;Kim, Young-Hoon;Hong, Jin;Sheen, Yhun Yhong
    • Mass Spectrometry Letters
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    • v.10 no.1
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    • pp.18-26
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    • 2019
  • We developed a bioanalytical method for simultaneous determination of nine NBOMe derivatives (25H-NBOMe, 25B-NBOMe, 25E-NBOMe, 25N-NBOMe, 25C-NBOH, 25I-NBOH, 25B-NBF, 25C-NBF, and 25I-NBF) in human plasma using liquid chromatography tandem mass spectrometry (LC-MS/MS). Human plasma samples were pre-treated using solid-phase extraction. Separation was achieved on a C18 column under gradient elution using a mobile phase containing 0.1% formic acid in acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Mass detection was performed in the positive ion mode using multiple reaction monitoring. The calibration range was 1-100 ng/mL for all quantitative analytes, with a correlation coefficient greater than 0.99. The intra- and inter-day precision and accuracy varied from 0.85 to 6.92% and from 90.19 to 108.69%, respectively. The recovery ranged from 86.36 to 118.52%, and the matrix effects ranged from 27.09 to 99.72%. The stability was acceptable in various conditions. The LC-MS/MS method was validated for linearity, accuracy, precision, matrix effects, recovery and stability in accordance with the FDA guidance. The proposed method is suitable for reliable and robust routine screening and analysis of nine NBOMe derivatives in forensic field.