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http://dx.doi.org/10.5478/MSL.2014.5.3.79

A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation  

Kang, Myung Joo (College of Pharmacy, Dankook University)
Cho, Ha Ra (College of Pharmacy, Dankook University)
Lee, Dong Hoon (College of Pharmacy, Chung-Ang University)
Yeom, Dong Woo (College of Pharmacy, Chung-Ang University)
Choi, Young Wook (College of Pharmacy, Chung-Ang University)
Choi, Yong Seok (College of Pharmacy, Dankook University)
Publication Information
Mass Spectrometry Letters / v.5, no.3, 2014 , pp. 79-83 More about this Journal
Abstract
Three different dutasteride extraction methods were compared and a method based on liquid-liquid extraction (LLE) using methyl tert-butyl ether and methylene chloride was proved to be more effective than others for the extraction of dutasteride and finasteride, the internal standard (IS), from rat plasma. Additionally, a method composed of the LLE extraction, liquid chromatography, and multiple reaction monitoring (MRM) to target dutasteride and IS was validated by assessing specificity, linearity ($r^2$ = 0.9993, 5 - 400 ng/mL), sensitivity (the limit of detection: 4.03 ng/mL; the limit of quantitation: 12.10 ng/mL), accuracy (intra-day: 89.4 - 105.9%; inter-day: 84.9 - 100.9%), precision (intra-day: 0.8 - 6.9%; inter-day: 2.9 - 15.9%), and recovery (84.7 - 107.8%). Since the validated method was successfully applied to a pharmacokinetic study of dutasteride, it can be useful for the pharmacokinetic evaluation of newly developed dutasteride formulations.
Keywords
dutasteride; multiple reaction monitoring; rat plasma;
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  • Reference
1 Gisleskog, P. O.; Hermann, D.; Hammarlund-Udenaes, M.; Karlsson, M. O. Br. J. Clin. Pharmacol. 1999, 47, 53.
2 Evans, H. C.; Goa, K. L. Drugs Aging 2003, 20, 905.   DOI   ScienceOn
3 Gaines, K. K. Urol. Nurs. 2003, 23, 218.
4 Brown, C. T.; Nuttall, M. C. Int. J. Clin. Pract. 2003, 57, 705.
5 Olsson, G. P.; Hermann, D.; Hammarlund-Udenaes, M.; Karlsson, M. O. Eur. J. Pharm. Sci. 1999, 8, 291.   DOI   ScienceOn
6 U. S. Food Drug Administration, "Application Number: 21-319.": , cited 27 Jun, 2012.
7 Ofner, C. M. 3rd.; Zhang, Y. E.; Jobeck, V. C.; Bowman, B. J. J. Pharm. Sci. 2001, 90, 79.   DOI
8 Ramakrishna, N. V. S.; Vishwottam, K. N.; Puran, S.; Koteshwara, M.; Manoj, S.; Santosh, M. J. Chromatogr. B 2004, 809, 117.   DOI   ScienceOn
9 Gomes, N. A.; Pudage, A.; Joshi, S. S.; Vaidya, V. V.; Parekh, S. A.; Tamhankar, A. V. Chromatographia 2009, 69, 9.   DOI   ScienceOn
10 Baek, I. -H.; Kim, M. -S. Chem. Pharm. Bull. 2012, 60, 1468.   DOI
11 Contractor, P.; Kurani, H.; Guttikar, S.; Shrivastav, P. S. Biomed. Chromatogr. 2013, 27, 1168.   DOI   ScienceOn
12 Olsen, E. A.; Hordinsky, M.; Whiting, D.; Stough, D.; Hobbs, S.; Ellis, M. L.; Wilson, T.; Rittmaster, R. S.; Dutasteride Alopecia Research Team. J. Am. Acad. Dermatol. 2006, 55, 1014.   DOI   ScienceOn