• Title/Summary/Keyword: evaluation guideline

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Development of test method for the evaluation of pesticide acute toxicity using earthworm(Lumbricus rubellus) (붉은지렁이 (Lumbricus rubellus)를 이용한 농약의 급성독성 시험법 개발)

  • Park, Yeon-Ki;Park, Kyeong-Hoon;Kim, Byung-Seok;Kyung, Kee-Sung;Shin, Jin-Sup;Oh, Byung-Youl
    • The Korean Journal of Pesticide Science
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    • v.4 no.4
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    • pp.56-60
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    • 2000
  • A study was performed to determine the maintenance conditions of the earthworm, Lumbricus rubellus, for the acute toxicity test. To fine out climatic and soil conditions, the earthworms were maintained in artificial soil consisting of sand, clay mineral and peat at different levels of conditions for 14 days. Lumbricus rubellus led to an increase of biomass at temperature $22{\pm}2^{\circ}C$, soil pH $7.0{\pm}1$ and moisture 40%. And four fungicides were tested for acute toxicities to Lumbricus rubellus, according to the optimum condition. The test earthworms were exposed to each pesticide with various concentration gradients. After 14 days, the number of surviving earthworms and their weight alteration during the test period was determined. The 14-day $LC_{50}$ values for the Lumbricus rubellus, of carbendazim, benomyl, thiophanate-methyl and thiabendazole were determined to be 59, 53, 64 and 36 mg/ kg soil dry weight, respectively.

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Detection of HER2 Status in Breast Cancer: Comparison of Current Methods with MLPA and Real-time RT-PCR

  • Pazhoomand, Reza;Keyhan, Elahe;Banan, Mehdi;Najmabad, Hossein;Karimlou, Masoud;Khodadad, Faranak;Iraniparast, Alireza;Feiz, Farnaz;Majidzadeh, Keivan;Bahman, Ideh;Moghadam, Fatemeh Aghakhani;Sobhani, Atoosa Madadkar;Abedin, Seyedeh Sedigheh;Muhammadnejad, Ahad;Behjat, Farkhondeh
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7621-7628
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    • 2013
  • Human epidermal growth factor receptor (HER) status is an important prognostic factor in breast cancer. There is no globally accepted method for determining its status, and which method is most precise is still a matter of debate. We here analyzed HER2 mRNA expression by quantitative reverse transcription-PCR (qRT-PCR) and HER2 DNA amplification using multiplex ligation-dependent probe amplification (MLPA). In parallel, we performed a routine evaluation of HER2 protein by immunohistochemistry (IHC). To assess the accuracy of the RT-PCR and MLPA techniques, a combination of IHC and fluorescence in situ hybridization (FISH) was used, substituting FISH when the results of IHC were ambiguous (2+) and for those IHC results that disagreed with MLPA and qRT-PCR, this approach being termed IHC-FISH. The IHC results for four samples were not compatible with the MLPA and qRT-PCR results; the MLPA and qRT-PCR results for these samples were confirmed by FISH. The correlations between IHC-FISH and qRT-PCR or MLPA were 0.945 and 0.973, respectively. The ASCO/CAP guideline IHC/FISH correlation with MLPA was (0.827) and with RT-PCR was (0.854). The correlations between the IHC results (0, 1+ as negative, and 3+ as positive) and qRT-PCR and MLPA techniques were 0.743 and 0.831, respectively. Given the shortcomings of IHC analysis and greater correlations between MLPA, qRT-PCR, and FISH methods than IHC analysis alone with each of these three methods, we propose that MLPA and real-time PCR are good alternatives to IHC. However a suitable cut-off point for qRTPCR is a prerequisite for determining the exact status of HER2.

Isokinetic Evaluation of the Trunk Flexors and Extensors for the White Collar Workers in Adult Males (사무직근로자의 요추부 굴곡근 및 신전근의 등속성 근력평가 서울시 일부지역의 21세 $\sim$ 49세 남자직장인을 중심으로)

  • Oh, Seung-Kil;Choi, Byung-Ok
    • Journal of Korean Physical Therapy Science
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    • v.7 no.1
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    • pp.377-396
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    • 2000
  • After warming-up exercise for 20 minutes, Isokinetic measurement of trunk strength for flexor and extensor was done by using Cybex 6000 TEF Unit on 91 healthy male white workers from 22years old to 49 years old, and compared each other. 20 repetitions of trunk extension-flexion were done at $120^{\circ}$/sec angular velocity. After resting for 1 minutes, Four repetitions at two different angular velocities($60^{\circ}$/sec, $120^{\circ}$/sec) were done with 30 seconds of resting interval between each angular velocity. The purpose of this study is to obtain the isokinetic normative strength values for trunk extensors and flexors, and is to know the correlation between age, height, weight of subjects and data from isokinetic trunk strength measurement, and is to provide a guideline for exercise program of male white collar workers The collected data were analyzed by ANOVA, Duncan's Multiple Range Test, and Pearson correlation coefficiency in PC-SAS program. The results obtained were as follow; 1. There is significant positive-correlation with the statistic value between weight and peak torque of trunk muscles at two different angular velocities($60^{\circ}$/sec, $120^{\circ}$/sec)(p<01), between height and peak torque of trunk muscles at two different angular velocities($60^{\circ}$/sec, $120^{\circ}$/sec) except peak torque of trunk flexor at $60^{\circ}$/sec(p<01). 2. There is nagitive-correlation between age and peak torque of trunk muscles at two different angular velocities($60^{\circ}$/sec, $120^{\circ}$/sec), there is significant differences with statistic value between age and peak torque of trunk extensor at $120^{\circ}$/sec(p<.01). 3. Mean peak torque and mean peak torque % by body weight of trunk extensor is 1.1 times higher values than trunk flexor at $60^{\circ}$/sec. 4. There is the increase in peak torque angle of trunk flexor with increasing of age, and the decrease in peak torque angle of trunk flexor with increasing of age at two different angular velocities($60^{\circ}$/sec, $120^{\circ}$/sec). there is significant differences with statistic value in peak torque angle of trunk flexor at $120^{\circ}$/sec(p<.01). 5. There is significant decrease in endurance ratio of trunk extensor with increasing of age at $120^{\circ}$/sec(p<.01). In conclusion, peak torque of trunk extensor is 1.1 times higher values than trunk flexor in healthy male white collar workers.

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Korean University Students' Attitude toward a Task Recording Activity : Based on the TOEIC Speaking Test (한국대학생의 과업녹음활동에 대한 태도연구 : 토익스피킹 시험을 기반으로)

  • Im, Hee-Joo
    • The Journal of the Korea Contents Association
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    • v.14 no.7
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    • pp.550-558
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    • 2014
  • The purposes of the study are to introduce and apply an activity that can help improve student-initiated learning on the TOEIC Speaking Test (TOEICST) performance and to see students' attitude toward the activity. Twenty-eight university students in Chungcheong province engaged in learning based on this activity. In the present study, the activity proposed by McCormick and Vercellotti (2013) and Stillwell, Curabba, Alexander, Kidd, Kim, Stone, & Wyle (2010) was modified and adapted[14][19]. The outline of the Task Recording Activity (TRA) consisted of three steps: preparation step, Task Recording Activity step, and evaluation step. As data collection of the study, a survey and students' journal were utilized and qualitatively analyzed. The results showed that student-initiated learning activity could be applied positively not only in communicative lessons, but in TOEICST specific lessons as well. Additionally, participants showed positive attitudes toward self-transcription and self-correction. Some implications of the study are that the TRA can be applied to generate autonomous learners studying for the TOEIC Speaking Test, that the guideline for different proficiency can be developed to help them improve their self-reflection, and that students need to have an active attitude to lead their own learning.

A Vibration Evaluation and Improvement Scheme for Open Test Blasting (노천시험발파의 진동평가와 개선방안)

  • Kim, Eung-Rok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.1
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    • pp.310-315
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    • 2017
  • Although studies evaluating the effects of the blasting vibration on the adjacent structures from various angles have continued, cases of securing the safety of the adjacent buildings and researching the proper blasting method for the field condition by analyzing the vibration waveform of the measuring field while performing the open pit blasting are poor. Therefore, it is necessary to present a remedy for blasting pattern selection through test blasting that is appropriate for field conditions, and is economical and efficient. In this study, open pit blasting work was conducted based on the separation distance applied according to the standard blasting method by test blasting and the vibration regulation standard in the road expansion construction site to measure the blasting vibration value, and the vibration prediction equation by blasting methods was examined using a regression analysis computer program to calculate K, N, and R of the confidence level 95%. By setting the blasting allowed vibration standard of the test blasting target area to 0.3cm/sec, and the charring weight and blasting method by the separation distances according to the blasting vibration estimation equation of the open pit blasting guideline and the blasting vibration estimation equation of the test blasting were compared/analyzed, it was possible to identify the factors that increased the working expenses. In addition, the measurement and analysis of the adjacent structures during open pit blasting and the blasting vibration were performed after selecting the most adjacent structure to the open pit blasting spot to analyze the problems on the test blasting procedure and analysis method in the open pit blasting design/construction guidelines, which appeared in the process of completing open pit blasting construction, and a remedy is presented.

Motility Analysis of Gate Myocardium SPECT Image Using Left Ventricle Myocardium Model (좌심실 심근 모델을 이용한 게이트 심근 SPECT 영상의 운동성 분석)

  • 손병환;김재영;이병일;이동수;최흥국
    • Journal of Korea Multimedia Society
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    • v.6 no.3
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    • pp.444-454
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    • 2003
  • An analysis of heart movement is to estimate a role which supplies blood in human body. We have constructed a left ventricle myocardium model and mathematically evaluated the motion of myocardium. The myocardial motility was visualized using some parameters about cardiac motion. We applied the myocardium model in the gated myocardium SPECT image that showed a cardiac biochemical reaction, and analyzed a motility between the gated myocardium SPECT image and the myocardium model. The myocardium model was created of the based on three dimensional super-ellipsoidal model that was using the sinusoidal function. To express a similar form and motion of the left ventricle myocardium, we calculated parameter functions that gave the changing of motion and form. The LSF algorithm was applied to the myocardium gated SPECT image data and the myocardium model, and finally created a fitting model. Then we analyzed a regional motility direction and size of the gated myocardium SPECT image that was constructed on a fitting model. Furthermore, we implemented the Bull's Eye map that had evaluated the heart function for presentation of regional motility. Using myocardium's motion the evaluation of cardiac function of SPECT was estimated by a contraction ability, perfusion etc. However, it is not any estimation about motility. So, We analyzed the myocardium SPECT's motility of utilizing the myocardium model. We expect that the proposed algorithm should be a useful guideline in the heart functional estimation.

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Improvement of Atmospheric PM2.5 Levels and Related Premature Deaths in Seoul, Korea (서울시 대기 중 PM2.5 농도 개선과 조기사망 감소 효과)

  • Bae, Hyun-Joo;Shin, JI-Young;Park, Chan-Koo;Jung, Kweon;Lee, Sang-Yol;Kim, Min-Young;Park, Jeong-Im
    • Journal of Korean Society for Atmospheric Environment
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    • v.26 no.1
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    • pp.10-20
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    • 2010
  • In recent studies, $PM_{2.5}$ has been reported to be more harmful to human health than $PM_{10}$ because it penetrates more deeply into the lung. We estimated $PM_{2.5}$ related health benefits in Seoul from implementing the World Health Organization (WHO)'s guidelines (annual average $10{\mu}g/m^3$, 24-hour average $25{\mu}g/m^3$) and U.S. Environmental Protection Agency (EPA)'s National Ambient Air Quality Standard (annual average $15{\mu}g/m^3$, 24-hour average $35{\mu}g/m^3$). U.S. EPA's Environmental Benefits Mapping and Analysis Program was utilized for the analysis. It was predicted that the attainment of the WHO annual guideline and U.S. EPA's annual standard, relative to the concentration in 2006, would result in reduction of 2,333~2,895 premature deaths and 1,703~2,121 premature deaths, respectively. If the WHO and EPA's daily standard for $PM_{2.5}$ are attained, 1,211~1,394 and 1,012~1,165 premature deaths could be avoided, respectively. Sensitivity analyses indicated that the estimates were robust regardless of air quality simulation methods for attaining the $PM_{2.5}$ goals. This study provides a quantitative approach to evaluate health risks from air pollution as well as to assess the potential health benefits of improving atmospheric $PM_{2.5}$ concentration. Even considering the intrinsic limitations and uncertainties of the analysis, it is an important information to rationalize the enforcement of $PM_{2.5}$ management policies and measures in Seoul, Korea.

Evaluation of Adhesion Characteristics of Crack Sealants Used in Asphalt Concrete Pavement (아스팔트 콘크리트 포장용 균열실링재의 부착특성 평가)

  • Lee, Jae-Jun;Kim, Seung-Hoon;Baek, Jong-Eun;Lim, Jae-Kyu;Kim, Yong-Joo
    • International Journal of Highway Engineering
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    • v.17 no.2
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    • pp.55-62
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    • 2015
  • Cracking is an inevitable fact of asphalt concrete pavements and plays a major role in pavement deterioration. Pavement cracking is one of the main factors determining the frequency and method of repair. Cracks can be treated with a number of preventative maintenance actions, including overlay surface treatments such as slurry sealing, crack sealing, or crack filling. Pavement cracks can show up as one or all of the following types: transverse, longitudinal, fatigue, block, reflective, edge, and slippage. Crack sealing is a frequently used pavement maintenance treatment because it significantly extends the pavement service life. However, crack sealant often fails prematurely due to a loss of adhesion. Because current test methods are mostly empirical and only provide a qualitative measure of the bond strength, they cannot accurately predict the adhesive failure of the sealant. This study introduces a laboratory test aimed at assessing the bonding of hot-poured crack sealant to the walls of pavement cracks. A pneumatic adhesion tensile testing instrument (PATTI) was adopted to measure the bonding strength of the hot-poured crack sealant as a function of the curing time and temperature. Based on a limited number of test results, the hot-poured crack sealants have very different bonding performances. Therefore, this test method can be proposed as part of a newly developed performance-based standard specification for hot-poured crack sealants for use in the future. PURPOSES : The purpose of this study was to evaluate both the adhesion and failure performance of a crack sealant as a function of its curing time and curing temperature. METHODS: A pneumatic adhesion tensile testing instrument (PATTI) was adopted to measure the adhesion performance of a crack sealant as a function of the curing time and curing temperature. RESULTS: With changes in the curing time, curing temperature, and sealant type, the bond strengths were found to be significantly different. Also, higher bond strengths were measured at lower temperatures. Different sealant types produced completely different bond strengths and failure behaviors. CONCLUSIONS: The bonding strength of an evaluated crack sealant was shown to differ depending on various factors. Two sealant types, which were composed of different raw materials, were shown to perform differently. The newly proposed test offers the possibility of evaluating and differentiating between different crack sealants. Based on alimited number of test results, this test method can be proposed as part of a newly developed performance-based standard specification for crack sealants or as part of a guideline for the selection of hot-poured crack sealant in the future.

An Influence of Unit-Water Content Distribution in Ready-Mixed Concrete on Strength and Durability of Concrete (레미콘 단위수량 산포가 콘크리트 강도 및 내구성에 미치는 영향)

  • Woo, Young-Je;Lee, Han-Seung;Jung, Sang-Hwa
    • Journal of the Korea Concrete Institute
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    • v.20 no.3
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    • pp.375-381
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    • 2008
  • Various problems such as durability degradation may happen when extra water is added to concrete. Because of these reasons, the change of water content is managed by using rapid evaluation method of unit water content such as electric capacity method, heat drying method making use of micro wave, unit capacity mass method among various methods. Especially, in Japan, guidance for the change of water content ($\pm$ 10, 15, 20 kg/$m^3$ etc.) were regulated and used. However, it is the real situation that the guidance which were regulated in South Korea evaluate suitability only considering production and measurement error under the circumstances which are not considering the degree of durability degradation. Therefore, this study tries to investigate the influence of addition of extra water in the concrete on the durability degradation of concrete when it was added by artificial manipulation or by management error. From the test results, a guideline of the contents of extra water for the quality control is suggested with the consideration of the degree of durability degradation and the probable error resulted from the addition of extra water. The contents of extra water for tests are set as 0, 15, 25, 35 kg/$m^3$. To examine the durability degradation of concrete, freezing and thawing, carbonation, chloride penetration and compressive strength are tested.

Calculation of the Peak-hour Ratio at Urban Railway Stations Reflecting Passenger Demand Pattern and Land Use Inventory - A Case of Seoul - (승객 수요 패턴과 역세권의 토지이용 특성을 반영한 도시철도역 첨두시간 집중률 산정 - 서울시를 대상으로 -)

  • Jang, Sunghoon;Kim, Hyo-Seung;Lee, Chungwon;Kim, Dong-Kyu
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.4
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    • pp.1581-1589
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    • 2013
  • The aim of this study is to suggest a methodology for calculating the peak-hour ratio of passengers at urban railway stations by reflecting the characteristics of passenger demand patterns and the land use inventory of stations. To achieve this, urban railway stations in Seoul are divided into three groups by using factor analysis and cluster analysis. For each station group, we calculate five and four variables related to the passenger demand patterns and the land use inventory of stations, respectively, as well as the peak-hour ratios of passengers. Among these nine variables, average daily passengers and the location quotient (LQ) index for business services are selected as the classification criteria for station groups based on statistical tests. Using the two variables, a group allocation process is suggested to estimate the peak-hour ratio of passengers for a newly-constructed station. Evaluation results based on thirteen stations show that the proposed methodology produces lower errors than the currently-used guideline does. The results of this study contribute to establishing efficiently construction and operation plans for newly-constructed stations.