• Archives of Pharmacal Research
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• 제20권1호
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• pp.91-92
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• 1997

In the course of continous work on tubers of Gastrodia elata, a new constituent, 4, 4$^{I}$-dihydroxybenzyl sulfoxide was isolated from the ethyl acetate soluble fraction prepared from the methanol extract. The structure of the compound was identified from the elemental analytical and spectroscopic data in comparison with those of non-substituted benzyl sulfoxide.e.

• 생약학회지
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• 제30권1호
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• pp.84-86
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• 1999

From the ethyl acetate fraction of the methanol extract of the fruits of Opuntia ficus-indica var. saboten, two dihydroflavonois were isolated and identified as (+)-trans-dihydrokaempferol (1) and (+)-trans-dihydroquercetin (2) by spectroscopic methods.

• 한국자원식물학회지
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• 제3권1호
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• pp.64-70
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• 1990

The physicochemical activities of the underground parts of Roso rugosa Thunb (Rosaceae) , which have been used as an antidiabetic in the folkmedicines of Korea , was carried outThe methanol extract from this plant reduced serum Lipid Level the normal rat . When various fractions from the methanol extract was administered to the rat treated with 1% choholesterol and 0.5% cholic aciddiet , the ethyl acetate soluble fraction lowered the serum triglycerideand phospholipid level most remarkably.

• Journal of Ginseng Research
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• 제14권1호
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• pp.27-29
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• 1990

A Polyphenolic Compound was isolated from ethyl acetate fraction of Panax ginseng. And the chemical structure of its permethyl ether was suggested as 6-[2(3,4-dimethoxyphenyl)ethenyl]-5-(3,4-dimethoxyphenyl)2,8,10-trimethoxybenzo[a] xanthen-9-one by spectroscopic and chemical degradative evidences.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2000년도 제29회 국제학술심포지움 및 추계학술대회
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• pp.110-110
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.385.1-385.1
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• 2002

Cordyceps has been used as a tonic for replenishing vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the kidney function, MeOH Ex. and its hexane. ethyl acetate, butanol and water fractions of cultivated Cordyceps pruinosa on mercuric chloride induced renal failure rats were investigated. Urin volume. blood parameters(urea nitrogen. uric acid. creatinine) and urinary electrolytes content (natrium. potassium. chloride) were determined. MeOH extract and butanol fraction showed diuretic effect. (omitted)

• 대한화장품학회지
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• 제34권2호
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• pp.101-107
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• 2008

신선초 추출물 및 그의 분획물의 미백 효과를 알아보기 위하여, B16 멜라노마와 정상 사람 표피 세포를 이용하여 멜라닌 생성에 관련된 다양한 실험을 실시하였다. 70% ethanol 용매로 추출한 신선초 추출물을 연속적으로 분획하여 hexane, ethyl acetate, n-butanol, 물 분획물을 얻었다. 버섯 티로시나제 활성 실험에서 신선초 추출물 및 그의 분획물은 저해 효과를 보이지 않았으나, B16 멜라노마를 이용한 멜라닌 생성 실험에서는 hexane 분획물과 ethyl acetate 분획물에서 억제 효과를 보였다. 세포내 멜라닌 생성의 초기 단계에 작용하는 티로시나제 단백질의 활성을 조사한 결과, hexane 분획($IC_{50}$ < $25{\mu}g/mL$)과 ethyl acetate분획($IC_{50}$ < $5{\mu}g/mL$)은 알부틴($IC_{50}{\leq}250{\mu}g/mL$)과 비교 시 더 낮은 농도에서 현저하게 티로시나제 활성을 저해시켰다. 따라서 신선초 추출물의 미백 작용 기전을 규명하기 위하여, 멜라노 사이트의 mitogen으로 작용하는 endothelin-1(ET-1)과 UVB에 의해 생성되어 염증을 매개하는 interleukin-$1{\alpha}$(IL-$1{\alpha})$의 생성량을 측정하였다. Hexane 및 ethyl acetate 분획물은 IL-$1{\alpha}$의 생성에 영향을 끼치지 못했으나, 표피세포에서 UVB에 의해 생성된 ET-1을 농도에 따라 감소시킴으로써 멜라노사이트의 melanogenesis를 저해하는 것으로 확인되었다. 따라서 신선초 추출물은 미백 효능을 갖는 화장품 소재로써 사용 가능하리라 판단된다.

• Toxicological Research
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• 제18권2호
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• pp.117-127
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• 2002

Saururus chinensis Baill has been used in Korean folk medicine for the treatment of various diseases such as edema, Jaundice, and furuncle. The components of this plant were extracted into four fraction. Among the four fraction, hexane and ethyl acetate fraction were highly toxic to 3T3 mouse embryo fibroblast and Raw 264.7 mouse macrophage, but n-butanol and residue fraction did not show any toxic effect to those cell lines. n-Butanol and residue fraction exhibited antioxidant effects on hydro-gen peroxide, hydroxyl radical, and superoxide anion directly in vitro and in the 3T3 fibroblasts. All the four fractions inhibited lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) formation. In addition, n-butanol and residue fraction showed inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide production, and also down-regulated inducible nitric oxide synthase (iNOS) mRNA transcription 6 h after LPS stimulation in Raw 204.7 cells. Only n-butanol fraction, which mainly consists of flavonoids, inhibited NF-kB activation by decreasing IkBa degradation 90 min after LPS stimulation. horn the results, it is suggested that this plant could be a good candidate material for drug development based on its antioxidant and/or anti-inflammatory constituents.

• 한국환경농학회지
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• 제29권1호
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• pp.7-11
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• 2010

This experiment was conducted to investigate the growth inhibition effects caused by continuous cropping soil in peony(Paeonia lactiflora Pallas). The effect of extracts from continuous cropping soil of peony was tested with bio-assay method using callus cells induced from peony filament tissues and seedlings derived from peony zygotic embryos. The cell viability and seedling growth were significantly inhibited by methanol extract in continuous cropping soil. Methanol extract from continuous cropping soil was successively fractionated with solvents such as n-hexane, ethyl acetate, n-butanol and water. The seedling growth was inhibited by ethyl acetate fraction obtained in methanol extract.

• 생약학회지
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• 제38권3호통권150호
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• pp.258-262
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• 2007

In the present study, we investigated the antioxidant activity of methanol extract and its organic fractions (n-hexane, $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$) of Capsiccum annuum leaves against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. As results, methanol extract, and its ethyl acetate and n-butanol fractions showed good scavenging effect at 100 ${\mu}g/ml$. Four compounds were isolated from the ethyl acetate fraction through silica gel and Sephadex LH-20 column chromatographies. Their chemical structures were elucidated as apigenin (1), vanillic acid (2), uracil (3) and apigenin $7-O-{\beta}-D-apiofuranosyl(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (4) by comparison of spectral data with those in reference. $IC_{50}$ value of compound 2 was 14.7 ${\mu}g/ml$, while compounds 1, 3 and 4 had no effect on the DPPH radical.