Browse > Article

Phenolic Compounds from Capsiccum annuum Leaves Showing Radical Scavenging Effect  

Choi, Jang-Gi (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Hur, Jong-Moon (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Cho, Hyun-Woo (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Park, Jong-Cheol (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.3, 2007 , pp. 258-262 More about this Journal
Abstract
In the present study, we investigated the antioxidant activity of methanol extract and its organic fractions (n-hexane, $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$) of Capsiccum annuum leaves against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. As results, methanol extract, and its ethyl acetate and n-butanol fractions showed good scavenging effect at 100 ${\mu}g/ml$. Four compounds were isolated from the ethyl acetate fraction through silica gel and Sephadex LH-20 column chromatographies. Their chemical structures were elucidated as apigenin (1), vanillic acid (2), uracil (3) and apigenin $7-O-{\beta}-D-apiofuranosyl(1{\rightarrow}2)-{\beta}-D-glucopyranoside$ (4) by comparison of spectral data with those in reference. $IC_{50}$ value of compound 2 was 14.7 ${\mu}g/ml$, while compounds 1, 3 and 4 had no effect on the DPPH radical.
Keywords
Capsiccum annuum; vanillic acid; antioxidant; DPPH;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 김소자 (1999) 전통 고추장의 암예방 기능성, 박사학위논문, 동덕여자대학교 대학원
2 Kawaguci, Y., Ochi, T., Takaishi, Y., Kawazoe, K. and Lee, K. H. (2004) New sesquiterpenes from Capsicum annuum. J. Nat. Prod., 67: 1893-1896   DOI   ScienceOn
3 Davis, B. H., Mathews, S. and Kirk, J. T. O. (1970) The nature and biosynthesis of the carotenoids of different colour varieties of Capsicum annuum. Phytochemistry, 9: 797-805   DOI   ScienceOn
4 Park, K. Y., Lee, K. I. and Rhee, S. H. (1992) Inhibitory effect of Green-yellow vegetables on the mutagenicity in Salmonella assay system and on the growth of AZ-521 human gastric cancer cells. J. Korean Soc. Food Nutr., 21: 149-153   과학기술학회마을
5 Kim, J. H., Jeong, C. H. and Shin, K. H. (2003) Biological activities of solvent fractions of Capsicum annuum leaves. Korean J. Food Preserv., 10: 540-546   과학기술학회마을
6 He, X. and Liu, R. H. (2006) Carnberry phytochemicals: isolation, structure elucidation, and their antiproliferative and antioxidant activities. J. Agric. Food Chem., 54: 7069-7074   DOI   ScienceOn
7 Lee, S. H., Kang, S. S. and Shin, K. H. (2002) Coumarins and a pyrimidine from Angelica gigas roots. Natural Product Sciences, 8: 58-61
8 Kang, K. S., Kim, H. Y., Pyo, J. S. and Yokoawa, T. (2006) Increase in the free radical scavenging activity of ginseng by heat-processing. Biol. Pharm. Bull., 29: 750-754   DOI   ScienceOn
9 Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M. and Telser, J. (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 39: 44-84   DOI   ScienceOn
10 정보섭, 신민교 (1998) 도해향약(생약)대사전, 820-822. 영림사, 서울
11 Materska, M., Piacente, S., Stochmal, A., Pizza, C., Oleszek, W. and Perucka, I. (2003) Isolation and structure elucidation of flavonoid and phenolic acid glycosides from pericarp of hot pepper fruit Capsicum annuum L. Phytochemistry, 63: 893-898   DOI   ScienceOn
12 Tomas, F. and Ferreres, F. (1980) Flavonoids from the leaves of Capsicum annuum (Solanaceae). I. Major component. Afinidad, 37: 517-518
13 Materska, M. and Perucka, I. (2005) Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.). J. Agric. Food Chem., 53: 1750- 1756   DOI   ScienceOn
14 강소신의학원 (1998) 중약대사전, 871-873. 도서출판 정담, 서울
15 Kang, K. S., Yokozawa, T., Kim, H. Y. and Park J. H. (2006) Study on the nitric oxide scavenging effects of ginseng and its compounds. J. Agric. Food Chem., 54: 2558-2562   DOI
16 Ra, K. S., Jeong, S. C., Suh, H. J., Park, H. S., Baik, H. S., Choi, J. W. and Lee, Y. S. (2002) Isolation and characterization of complement system activating polyscaccharides from the hot water extract of the leaves of Capsicum annuum L. Korean J. Life Sci., 12: 87-95   DOI
17 Yahara, S., Kobayashi, N., Izumitani, Y. and Nohara, T. (1991) Solanaceous plants. XXIII. New acyclic diterpene glycosides, capsianosides VI, G and H from the leaves and stems of Capsicum annuum L. Chem. Pharm. Bull., 39: 3258-3260   DOI
18 Cornelius, C. W. (1999) Flavonoid glycosides from Crotalaria podocarpa. Phytochemistry, 51: 705-707   DOI   ScienceOn
19 Berke, T. G. and Shieh, S. C. (2001) Capsicum, chillies, paprika, bird's eye chilli. In Peter K. V. (ed.), Handbook of herbs and spices (Vol. 1), 111-122, Woodhead Publishing Ltd., Cambridge
20 Han, X. H., Hong, S. S., Hwang, J. S., Lee, M. K., Hwang, B. Y. and Ro, J. S. (2007) Monoamine oxidase inhibitory components from Cayratia japonica. Arch. Pharm. Res., 30: 13-17   과학기술학회마을   DOI
21 Li, T. and Yu, J. (1998) Studies on the chemical constituents of the leaves from Artabotrys hexapetalus. Yao Xue Xue Bao, 33: 591-596
22 Harbone, J. B. and Mabry, T. J. (1982) The flavonoids: advances in research, 41. Chapman and Hall, London
23 Yasuda, T., Takasawa A., Nakazawa T., Ueda J. and Ohsawa, K. (2003) Inhibitory effects of urinary metabolites on platelet aggregation after orally administering Shimotsu-To, a traditional Chinese medicine, to rats. J. Pharm. Pharmacol., 55: 239-244   DOI   ScienceOn
24 Park, J. C., Chung, S. K., Hur, J. M., Lee, J. H., Choi, M. R., Song, S. H. and Choi, J. W. (1997) Effects of the components and extracts of some edible and medicinal plants on the formation of lipid peroxide in rat liver homogenate. J. Korean Soc. food Sci. Nutr., 26: 1159-1163   과학기술학회마을