Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Saururus chinenesis Extracts Scavenge Reactive Oxygen Species and Modulate Nitric Oxide Production in Raw 264.7 Macrophages

  • Published : 2002.06.01
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Abstract

Saururus chinensis Baill has been used in Korean folk medicine for the treatment of various diseases such as edema, Jaundice, and furuncle. The components of this plant were extracted into four fraction. Among the four fraction, hexane and ethyl acetate fraction were highly toxic to 3T3 mouse embryo fibroblast and Raw 264.7 mouse macrophage, but n-butanol and residue fraction did not show any toxic effect to those cell lines. n-Butanol and residue fraction exhibited antioxidant effects on hydro-gen peroxide, hydroxyl radical, and superoxide anion directly in vitro and in the 3T3 fibroblasts. All the four fractions inhibited lipid peroxidation measured by thiobarbituric acid reactive substances (TBARS) formation. In addition, n-butanol and residue fraction showed inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide production, and also down-regulated inducible nitric oxide synthase (iNOS) mRNA transcription 6 h after LPS stimulation in Raw 204.7 cells. Only n-butanol fraction, which mainly consists of flavonoids, inhibited NF-kB activation by decreasing IkBa degradation 90 min after LPS stimulation. horn the results, it is suggested that this plant could be a good candidate material for drug development based on its antioxidant and/or anti-inflammatory constituents.

Keywords

References

  1. J.Agric.Food.Chem. v.50 no.5 Interactions between Flavonoids and Proteins;Effect on the Total Antioxidant Capacity Arts,M.J.;Haenen,G.R.;Wilms,L.C.;Beetstra,S.A.;Heijnen,C.G.;Voss,H.P.;Bast,A.
  2. J Lab Clin Med. v.107 Spontaneous hydrogen peroxide release from alveolar macrophages of some cigarette smokers Baughman,R.P.;Corser,B.C.;Strohofer,S.;Hedricks,D.
  3. Z.Ernahrungswiss v.37 no.2 Flavonols,flavone and anthocyanins as natural antioxidants of food and their possible role in the prevention of chronic diseases Bohm,H.;Boeing,H.;Hempel,J.;Raab,B.;Kroke,A.
  4. J.Biol.Chem v.276 no.7 Protein kinase Cdelta activation by interleukin-1 beta stabilizes inducible nitric-oxide synthase mRNA in pancreatic beta-cells Carpenter,L.;Cordery,D.;Biden,T.J.
  5. J.Biol.Chem. v.269 Molecular cloning structure, and chromosomal localization of the human inducible nitric oxide synthase gene Chartrain,N.A.;Geller,D.A.;Koty,P.P.;Sitrin,N.F.;Nussler,A.K.;Hoffman,E.P.;Billiar,T.R.;Hutchinson,N.I.;Mudgett,J.S.
  6. Planta Med. v.66 no.7 Effects of prenylated flavonoids and biflavonoids on lipopolysaccharide-induced nitric oxide production from the mouse macrophage cell line RAW 264.7 Cheon,B.S.;Kim,Y.H.;Son,K.S.;Chang,H.W.;Kang,S.S.;Kim,H.P.
  7. Toxicol.Appl.Pharmacol. v.166 Chlorophyllin suppression of lipopolysacchreide-induced nitric oxide production in RAW 264.7 cells Cho,K.J.;Han,S.H.;Kim,B.Y.;Hwang,S.G.;Park,K.K.;Yang,K.H.;Chung,A.S.
  8. Toxicol.Appl.Pharmacol. v.168 Effect of bioflavonoids extracted from the bark of Pinus maritima on proinflammatory cytokine interleukin-1 production in lipopolysaccharide-stimulated RAW 264.7 Cho,K.J.;Yun,C.H.;Yoon,D.Y.;Cho,Y.S.;Rimbach,G.;Parker,L.;Chung,A.S.
  9. Ann.N.Y.Acad.Sci. v.928 Inhibition mechanisms of bioflavonoids extracted from the bark of Pinus maritima on the expression of proinflammatory cytokines Cho,K.J.;Yun,C.H.;Packer,L.;Chung,A.S.
  10. Kyunghee University Hakwinonmun Phatmacological Studies on Saururs chinensis Baill Chung,J.O.;Kwon,C.D.
  11. Methods Enzymol. v.186 Determination of aldehydic lipid peroxidation products;malonaldehyde and 4-hydroxynonenal Esterbauer,H.;Cheeseman,K.H.
  12. Proc.Natl.Acad.Sci. v.89 Induction of calcium-independent nitric oxide synthase activity in primary rat glial cultures Galea,E.;Feinstein,D.L.;Reis,D.J.
  13. Proc.Natl.Acad.Sci. v.90 Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes Geller,D.A.;Lowenstein,C.J.;Shapiro,R.A.;Nussler,A.K.;Di Silvio,M.;Wang,S.C.;Nakayama,D.K.;Simmons,R.L.;Snyder,S.H.;Billiar,T.R.
  14. J.Immunol. v.156 Reactive oxygen intermediates as regulators of TNF-alpha production in rat lung inflammation induced by silica Gossart,S.;Cambon,C.;Orfila,C.;Seguelas,M.;Lepert,L.;Rami,J.;Carre,P.;Pipy,B.
  15. Haemostasis v.23 no.Suppl.1 The role of oxygen radicals in human disease, with particular reference to the vascular system Halliwell,B,
  16. Anal.Biochem. v.208 Purification of cysteine-rich bioactive peptides from leukocytes by continuous acid-urea-polyacryla-mide gel electrophoresis Harwig,S.S.;Chen,N.P.;Park,A.S.;Lehrer,R.I.
  17. Free Radic.Biol.Med. v.28 no.10 Reactive oxygen species, cell signaling, and cell injury Hensley,K.;Robinson,K.A.;Gabbita,S.P.;Salsman,S.;Floyd,R.A.
  18. Am.J Physiol. v.272 Nitric oxide synthase inhibition reverses arteriolar hyporesponsiveness to endothelin-1 in septic rats Hollenberg,S.M.;Piotrowski,M.J.;Parrillo,J.E.
  19. Ann.Acad.Med.Singapore v.27 Nitric oxide in septic shock;directions for future therapy Hon,W.M.;Khoo,H.E.;Moochhala,S.
  20. Cheju University Nomunjib v.33 Comparision on Volatilc Constituents of Houttuynia cordata and Saururus chinesis by GC and GC-MS Method Jung,D.S.
  21. Cheju University Nomunjib v.35 A Study on Fatty Acids and Amino Acids of Saururus chinesis Jung,D.S.
  22. Immunol.Cell.Biol. v.79 no.1 Elevation of intracellular calcium levels contributes to the inhibition of nitric oxide production by atrial natriuretic peptide Kiemer,A.K.;Vollmar,A.M.
  23. Chest v.106 Inhibition of nitric oxide synthesis improves the vasoconstrictive effect of noradrenaline in sepsis Landin,L.;Lorente,J.A.;Renes,E.;Canas,P.;Jorge,P.;Liste,D.
  24. J.Biol.Chem. v.268 Macrophage nitric oxide synthase gene;two upstream regions mediate induction by interferon-g and lipopolysaccharide Lowenstein,C.J.;Alley,E.W.;Raval,P.;Snowman,A.M.;Sunder,S.H.;Russell,S.W.
  25. J.Biol.Chem. v.267 Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line Lyons,C.R.;Orloff,G.J.;Cunningham,J.M.
  26. Pharmacol.Rev. v.43 Nitric oxide;physiology, pathophysiology and pharmacology Moncada,S.;Palmer,R.M.;Higgs,E.A.
  27. FEBS Lett. v.321 Diethyldithiocarbamate inhibits induction of macrophage NO synthase Mulsch,A.;Schray-Utz,B.;Mordvintcev,P.I.;Hauschildt,S.;Busse,R
  28. J.Assoc.Physicians india v.46 no.8 Antioxidant phenolics and flavonoids in common indian foods Nair,S.;Nagar,R.;Gupta,R.
  29. Cell v.78 Nitric oxide synthases;roles, tolls, and controls Nathan,C.;Xie,Q.A.
  30. Biochem.Mol.Biol.Int. v.42 no.1 Hydroxyl and superoxide anion radical scavenging activities of natural source antioxidants using the computerized JES-FR30 ESR specrtometer system Noda,Y.;Anzai,K.;Mori,A.;Kohno,M.;Shinmei,M.;Packer,L.
  31. Life Sci. v.68 NF-KappaB activation and IkappaB alpha dynamism involved in iNOS and chemokine induction in astroglial cells Nomura,Y.
  32. Biochem.Biophys.Res.Commun. v.191 Cloning on inducible nitric oxide synthase in rat vascular smooth muscle cells Nunokawa,Y.;Ishida,N.;Tanaka,S.
  33. Yakhak Hoeji v.41 Studies on the Cytotoxic Constitunts of Saururus chinesis($L_{OUR}$) $B_{AILL}$ Park,S.K.;O.G.;Bae,C.I.;Kim,H.J.;Han,W.S.;Chung,S.G.;Cho,E.W.
  34. FEBS Lett. v.506 no.1 Apigenin decreases expression of the myofibroblast phenotype Ricupero,D.A.;Poliks,C.F.;Rishikof,D.C.;Kuang,P.P.;Goldstein,R.H.
  35. Hepatology v.27 Stimulation of inducrible nitric oxide synthase in rat liver by hyaluronan fragments Rockey,D.C.;Chung,J.J.;Mckee,C.M.;Noble,P.W.
  36. Biochem.Biophys.Res.Commun. v.191 Pyrrolidine dithiocarbamate inhibits the induction of nitric oxide synthesis in rat alveolar macrophages Sherman,M.P.;Aeberhard,E.E.;Wong,V.Z.;Griscavage,J.M.;Ignarro,L.J.
  37. J.Nat.Prod. v.63 Hepatoprotective diastereomeric lignans from Saururus chinensis herbs Sung,S.H.;Kim,Y.C.
  38. J.Exp.Med. v.177 Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferong and baterial lipopolysaccharide Xie,Q.W.;Wishnan,R.;Nathan,C.
  39. J.Biol.Chem. v.269 Role of transcription factor NF-kB/Rel in induction of nitric oxide synthase Xie,Q.w.;Kashiwabara,Y.;Nathan,C.;