• Food Science and Biotechnology
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• 제18권3호
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• pp.788-792
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• 2009

Gamma $({\gamma})-irradiation$ can be used to control pathogens such as Vibrio vulnificus in seafood. The effects of irradiation on microbial cell populations (%) have been studied in order to develop detection methods for irradiated foods. The method used in this study was ethidium bromide monoazide (EMA) real-time polymerase chain reaction (PCR), using V. vulnificus specific primer, EMA, and $SYBR^{(R)}$ Green to discriminate between ${\gamma}-irradiated$ and non-irradiated cells. Confocal microscope examination showed that ${\gamma}-irradiation$ damaged portions of the cell membrane, allowing EMA to penetrate cells of irradidated V. vulnificus. ${\gamma}-Irradiation$ at 1.08 KGy resulted in log reduction ($-1.15{\pm}0.13$ log reduction) in genomic targets derived from EMA real-time PCR. The combination cold/heat shock resulted in the highest ($-1.74{\pm}0.1$ log reduction) discrimination of dead irradiated V. vulnificus by EMA real-time PCR.

• BMB Reports
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• 제31권4호
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• pp.384-390
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• 1998

DNA fragments (51-587 bp) were separated by capillary electrophoresis using entangled polymer, hydroxyethylcellulose, in uncoated fused silica capillary columns. The factors affecting the separation of DNA fragments with hydroxyethylcellulose media were evaluated, i.e., the concentration of buffer and entangled polymer, effects of additives (methanol, ethidium bromide, EDTA), temperature, and injection methods. Maximum performance was obtained by adding 5% methanol in 0.5% hydroxyethylcellulose solution at $30^{\circ}C$. Addition of methanol in polymer media increased the resolution of small size DNA fragments (< 100 bp). On the other hand, addition of ethidium bromide and EDTA, which are commonly used in conventional DNA separation, reduced the resolution of DNA fragments in the polymer solution. It turns out that the separation behavior of DNA in entangled polymer is more sensitive to the running condition compared to that in polyacrylamide gel-filled capillary, but the reproducibility of DNA separation in entangled polymer is reliable.

• Bulletin of the Korean Chemical Society
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• 제10권2호
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• pp.142-147
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• 1989

Fluorescence enhancements of ethidium bromide (EB) by solution species of low molecular weights such as DNA base molecules and nucleosides in water are reported. The degree of enhancements was determined by intensity as well as lifetime measurements for EB fluorescence. Experiments including solvent effects on absorbance and fluorescence spectra of EB, effects of protonation on the EB absorbance spectrum, and determination of equilibrium constants for EB-DNA bases have been performed to help explain the fluorescence enhancement. The results suggest that the excited state stabilization in the hydrophobic environment, the loss of torsional/vibrational energy of amino groups, and the change in the electronic transition characteristics are all responsible for the fluorescence enhancement.

• 미생물학회지
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• 제32권1호
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• pp.53-59
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• 1994

Agrobacterium tumefaciens KU12내에 존재하는 240kb 크기으 octopine형 Ti 플라스미드인 pTiKU12와 45kb 크기의 잠재 플라스미드인 pTi12를 제거하여 무독성의 A. tumefaciens 균주를 제조하였다. Octopine형 Ti 플라스미드인 pTiKU12는 고온(37${\circ}C$)에서의 배양과 ethidium bromide가 첨가되어 있는 배지에서의 배양을 각각 실시하여 제거하였으며, 잠재 플라스미드인 pTi12는 pTi12의 복제기원이 클로닝되어 잇는 재조합 플라스믿인 pYWXP와의 비화합성을 이용하여 제거하였다. pYWXP는 ethidium bromide가 첨가되어 있는 배지에서 고온(37${\circ}C$)으로 배양하여 제거하였다.

• 대한본초학회지
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• 제33권5호
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• pp.105-110
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• 2018

Objectives : We tried to observe the fluorescence anisotropy and intensity of ethidium ion in the intercalating binding interaction between DNA and ethidium ions in the presence of berberine, and then tried to explain the effect of berberine on the intercalating interaction of ethidium ion with DNA. Methods : DNA(calf thymus DNA), berberine and ethidium bromide(EtBr) were purchased from Sigma-Aldrich Co. Proper amount of each compound was dissolved in 20 mM sodium phosphate buffer(pH 7.0) containing 100 mM of NaCl to prepare stock solutions. Collections of the fluorescence anisotropy and intensity data were performed on JASCO FP-8300 spectrofluorometer equipped with a polarizer and a Peltier temperature controller. The excitation of ethidium ion was done at 550 nm and the emission data were collected at 600 nm. For Stern-Volmer plot, the fluorescence data were collected at $18^{\circ}C$ and $30^{\circ}C$. Results : According to the results of this research, the weak competitive binding pattern between ethidium ion and berberine appeared in binding with DNA at low ratio of DNA to ethidium ion. But at high ratio of DNA to ethidium ion, this weak competition disappeared. Instead, berberine might bind to DNA by intercalating way. In other words, berberine could de-intercalate ethidium ion from DNA at low concentration of DNA relative to ethidium ion, but could not at high concentration of DNA relative to ethidium ion. In addition, the mechanism of fluorescence quenching of ethidium ion could also proceed differently, depending on the ratio of the amount of DNA to that of ethidium ion. Conclusions : The effect of berberine on the DNA-ethidium ion intercalating interaction could work differently, depending on the relative ratio of the amount of DNA to that of ethidium ion. This study also showed that fluorescence anisotropy analysis is very useful method to obtain detailed information for investigation of the complex binding interactions. In order to fully understand the mechanism of action of the pharmacological effect by berberine, studies on the effect of berberine on the action of proteins such as various enzymes closely related to berberine-induced medicinal effects should be continued.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.290-291
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• 2011

• 한국미생물·생명공학회지
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• 제31권3호
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• pp.230-234
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• 2003

Small multidrug resistance(SMR) family(TC #2.A.7.1)에 속하는 막단백질 중 하나를 coding하는 Bacillus subtilis의 yvaE유전자의 발현을 유도한 결과, 발현된 YvaE단백질에 의해 대장균세포내에서 살균제를 포함한 다양한 양이온 약제에 대한 efflux활성을 촉매하는 것으로 확인되었다. 같은 operon내에 인접한 yaD유전자의 동시발현을 유도한 결과, 이러한 efflux활성은 억제가 되는 것으로 나타났다. Ethidium bromide를 기질로 하여 fluorimeter를 이용한 efflux transport실험결과, YvaE 단백질이 발현된 대장균세포의 경우 vector만을 함유한 대조세포에 비해 현저히 빠른 efflux활성을 보여주었다 따라서 YvaE 막단백질은 multidrug export를 촉매하는 SMR-type efflux pump임을 확인하였다.

• 한국식품영양학회지
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• 제1권2호
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• pp.64-67
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• 1988

Cadmium resistant cocobacillus B-17 from soil was tolerated up to 1600ug/ml of cadmium at agar plate and the strain B-17 was able to grow at 600ug/ml of cadmium at liquid medium after the lag phase being prolonged with lengthening culture time. Optimal pH of the strain was shown at pH7.0. The elimination frequency of cadmium resistance by 10ug/ml of acriflavin was 28%, and by 20ug/ml of ethidium bromide was 47%, respectively.

• Journal of Plant Biology
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• 제30권2호
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• pp.109-116
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• 1987

Effects of staining, buffer washing and denaturing agents on the transferrability of RNA fractionated on a methylmercury hydroxide-agarose gel to a nitrocellulose membrane were studied. Ethidium bromide staining and ammonium acetate buffer washing inhibited RNA transfer, while 3% HCHO and 0.5 M NaOH treatments stimulated transfer which was negated in the ammonium acetate buffer. Accordingly, maintenance of primary structure of RNA was proved to be essential for transferring RNA from the methylmercury hydroxideagarose gel to the nitrocellulose membrane.

• Bulletin of the Korean Chemical Society
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• 제12권3호
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• pp.262-264
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• 1991

An anomalous absorbance-temperature profile of calf thymus DNA, having a hypochromic trough just before the rise the $T_m$-region phase, occurs at the spermine concentration where the DNA collapses into a compact structure. The trough phase can be eliminated by the addition of ethidium bromide and also by a hydrophobic environment.