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Anomalous Absorbance-Temperature Profile of Calf Thymus DNA in Presence of Spermine

  • Chan-Yong Lee (Department of Biochemistry, Chungnam National University) ;
  • Hyeong-Won Ryu (Department of Biochemistry, Chungnam National University) ;
  • Moon-Jip Kim (Department of Physics, Soonchunhyang University) ;
  • Thong-Sung Ko (Department of Biochemistry, Chungnam National University)
  • Published : 1991.06.20

Abstract

An anomalous absorbance-temperature profile of calf thymus DNA, having a hypochromic trough just before the rise the $T_m$-region phase, occurs at the spermine concentration where the DNA collapses into a compact structure. The trough phase can be eliminated by the addition of ethidium bromide and also by a hydrophobic environment.

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References

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