• 제목/요약/키워드: ethanol toxicity

검색결과 300건 처리시간 0.023초

Protective Effects of Thiazolo[3,2-b]-1,2,4-Triazoles on Ethanol­Induced Oxidative Stress in Mouse Brain and Liver

  • Aktay Goknur;Tozkoparan Birsen;Ertan Mevlut
    • Archives of Pharmacal Research
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    • 제28권4호
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    • pp.438-442
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    • 2005
  • A series of 3-[1-(4-(2-methylpropyl) phenyl) ethyl]-1,2,4-triazole-5-thione (I) and its bicyclic condensed derivatives 6-benzylidenethiazolo[3,2-b]-1, 2,4-triazole-5(6H)-ones (IIa-IIf) were investigated for the prevention of ethanol-induced oxidative stress in liver and brain of mice. Administration of ethanol (0.1 mL/mice, p.o.) resulted in a drop of total thiol groups (T-SH) and non-protein thiol groups (NP-SH), and an increase in thiobarbituric acid reactive substances (TBARS) in both liver and brain tissue of mice (p<0.001). Among the compounds investigated (at a dose of 200 mg/kg, p.o.), I and IId ameliorated the peroxidative injury in these tissues effectively. Compounds IIa, IIc and IIe improved the peroxidative tissue injury only in brain. These findings suggest that certain condensed thiazolo-triazole compounds may contribute to the control of ethanol-induced oxidative stress in an organ selective manner.

Antioxidant and Anti-inflammatory Effects of Hot Water and Ethanol Extracts from Endemic Plants in Indonesia

  • Hwang, Jin-Woo;Choi, Jae-Ho;Kang, Sang-Moon;Lee, Sung-Gyu;Kang, Hyun
    • 대한의생명과학회지
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    • 제27권3호
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    • pp.161-169
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    • 2021
  • The present study, antioxidant and anti-inflammatory effects were measured of 6 endemic plants in Indonesian extracted by hot water or ethanol. The Nipa Fruticans Wurmb ethanol extract (NFWE) and Orthosiphon aristatus ethanol extract (OAE) showed the highest polyphenol and flavonoid contents of 203.70 and 33.70 ㎍/mg, respectively. Antioxidant activity of OAE was highest in DPPH radical scavenging activity (77.49% at 10 ㎍/mL) and ABTS+ radical scavenging activity (93.36% at 10 ㎍/mL). FRAP activity was significantly higher in NFWE than other extracts. Anti-inflammatory effects of 6 endemic plants in Indonesian extracted by hot water or ethanol were examined using nitric oxide (NO) inhibition assays. In the LPS-induced BV2 cells, OAE showed the highest inhibition of NO production without toxicity. The results of this study, suggest that OAE is a potential functional raw material for antioxidant and anti-inflammatory.

알코올에 대한 Escherichia coli, Clostridium acetobutylicum, Saccharomyces cerevisiae의 반응 (Cellular Responses to Alcohol in Escherichia coli, Clostridium acetobutylicum, and Saccharomyces cerevisiae)

  • 박주용;홍천상;한지혜;강현우;정봉우;최기욱;민지호
    • Korean Chemical Engineering Research
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    • 제49권1호
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    • pp.105-108
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    • 2011
  • 유가의 급등과 화석 연료에 의한 온난화 현상은 재생 가능한 대체 연료에 대한 필요성이 요구되었다. 수송용 바이오 연료를 비교하였을 때 에탄올보다 높은 알코올 경우 휘발유와 비슷한 장점을 갖는데 그 이유는 높은 에너지 밀도와 낮은 흡습성을 갖기 때문이다. 이러한 이유로 미생물의 발효는 지속적인 에너지를 얻을 수 있는 잠재적 생산자라 할 수 있다. 본 연구에서는 생물학적으로 생산되는 알코올 성분에 대하여 두 종의 세균과 한종의 효모인 Escherichia coli와 Clostridium acetobutylicum 그리고 Saccharomyces cerevisiae를 이용하여 바이오 알코올에 대한 세포 성장 정도와 함께 미생물내에 스트레스 반응 유전자들의 분석을 실시하였다. 분석한 알코올은 에탄올과 부탄올이며, 이들의 농도별 세균의 성장속도와 산화적 손상에 민감하게 반응하는 katG 유전자, 생물막 손상에 민감하게 반응하는 fabA 유전자, 단백질 손상에 민감하게 반응하는 grpE 유전자, 유전자 손상에 민감하게 반응하는 recA 유전자의 반응여부를 분석하였다. 그 결과, 에탄올과 부탄올 중 부탄올의 세포 독성이 더 높게 관찰되었으며, 부탄올의 경우 생물막 손상을 유발하는 세포내 독성효과를 지니고 있음을 확인하였다.

오리나무 수피엑스의 위염 및 위궤양에 대한 효과 (The Effect of Alnus japonica Cortex Extract on Gastric Lesion and Ulcer of Rats)

  • 정춘식;우병희;이은방;정기화
    • Biomolecules & Therapeutics
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    • 제4권1호
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    • pp.84-88
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    • 1996
  • Alnus japonica cortex has been used as antidiarrhea, antihemorrhage and the remedy of indigestion. This study was performed to investigate the effectiveness of the methanol extracts of the Alnus japonica cortex on the gastric lesion and ulcer. The methanol extract was fractionated with hexane, chloroform and butanol, followed by bioassay on antigastritic and antiulcer activity. The methanol extract showed low acute toxicity with minimum lethal dose of more than 5000 mg/kg, p.o. in mice. The chloroform and the butanol fraction reduced gastric lesion in HCI. ethanol induced gastritic model. On gastric secretion in pylorus ligated rat, the hexane and chloroform fraction decreased the volume and acidity. The butanol fraction had significant inhibitory effects on aspirin and Shay's ulcer. The butanol fraction showed a tendency to inhibit the decrease of mucin secretion due to ingestion of absolute ethanol.

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흰쥐와 개구리에 대한 Alcohol 독성의 비교 (A Comparison of Alcohol Toxicity on Rats and Frogs)

  • 유태석
    • 대한수의학회지
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    • 제15권2호
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    • pp.203-205
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    • 1975
  • The comparison of topic effects of methanol and ethanol on rats and frogs was performed. Intraperitoneal administration in graded doses of absolute alcohols showed specific toxicities on animals, suggesting that these substances had different effects on species specificity of animals. Throughout the experiment, the following results were obtained and summerized here. 1. In rats $LD_{50}$ dose of absolute methanol was $2.65{\mu}l/g$ of body weight, while that of absolute ethanol was $1.85{\mu}l/g$ of body weight. 2. The $LD_{50}$ dose of methanol in frogs was $17.2{\mu}l/g$ of body weight, while that of ethanol was $10.9{\mu}l/g$ of body weight.

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Microarray Analysis of Differential Gene Expression in Alcohol-Exposed Post Implantation Embryos

  • Kim, So-Hee;Rhee, Gyu-Seek;Kim, Soon-Sun;Sohn, Kyung-Hee;Kwack, Seung-Jun;Lee, Rhee-Da;Chae, Soo-Yeong;An, Sang-Mi;Hur, Man-Wook
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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    • pp.204-204
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    • 2002
  • Alcohol drinking during pregnancy can result in abnormal fetal development including fetal alcohol syndrome (FAS). The molecular mechanisms of FAS, however, is not completely elucidated. In the present study, we evaluated the developmental toxicity of ethanol and its metabolite, acetaldehyde using post implantation whole embryo culture and determined changes of gene expression by ethanol treatment by cDNA microarray.(omitted)

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Alcohol-induced hepatic fibrosis in pig

  • Lee, Chang-Woo;Jyeong, Jong-Sik;Lee, Cha-Soo;Jeong, Kyu-Shik
    • 한국동물위생학회지
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    • 제26권4호
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    • pp.345-359
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    • 2003
  • A number of toxicants have been incriminated as a causing hepatic disease. Among many detrimental injury, alcohol has been noted for hepatitis, fatty liver, fibrosis, and hepatic cirrhosis. The purpose of this study was to develop animal model for hepatic fibrosis in pigs fed ethanol, and to search for a new anti-fibrogenic agent via this model. Twelve male Landrace pigs were divided into 3 groups of 4 animals each. Group 1, 2 and 3 were fed with active ceramic water only, ceramic water + liquid diet containing 15% ethanol and normal tap water + liquid diet containing 15% ethanol for 12 weeks, respectively. At week 12, all pigs were immediately sacrificed for collection each tissue and blood. Serologically, serum ALT and AST levels were significantly reversed in group 2, as compared to group 3. They were normal range in pigs of group 1. Microscopically, macrovesicular lipid droplets and moderate hepatocellular necrosis were evident in the tap water + ethanol fed group 3. However, the active ceramic water treated group 1 showed normal architecture. Moreover, in group 2, mild fatty changes and necrosis were observed in hepatocytes. Collagen fibers were increased in spaces surrounding periportal and interlobular connective tissues in the group 3 of tap water + ethanol, but collagen synthesis and its thickness of fibrotic septa connecting portal tracts were markedly reduced in the group 2 of ceramic water + ethanol. Myofibroblasts were detected mainly in the interlobular connective tissues of pig liver of group 3 treated ethanol and tap water. Few to no myofibroblasts were observed in groups 1 and 2. CYP2E1 was not or rarely detected in group 1 fed ceramic water. However, group 2 showed slightly activation of CYP2E1 in the area of pericentral vein, while CYP2E1 was significantly activated in group 3 fed tap water and ethanol. Based on the above data, we believe that we have developed a unique alcohol induced fibrosis model in pig, which will be useful in developing anti-fibrotic agents and drugs. Furthermore, the active ceramic water used in our study had an inhibitory and may be protective against ethanol induced hepatic toxicity and fibrosis.

Aspartate 및 Asparagine 투여가 알코올 대사 및 중추신경계 효과에 미치는 영향 (Effect of Aspartate and Asparagine on Metabolism and Central Nervous System Effect of Alcohol in Healthy Male Volunteers)

  • 임동석;이경훈;장인진;신상구;이윤성;박상철
    • 대한약리학회지
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    • 제31권2호
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    • pp.261-269
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    • 1995
  • Background; To explore the efficacy of aspartate as NAD regenerating agent for ethanol and acetaldehyde oxidation, we performed crossover challenge in two groups of volunteers by coadministration of various doses of aspartate, asparagine and ethanol. Methods; 18 healthy male volunteers were randomly divided into two groups. 6 volunteers of the first group were administered 5 gm monosodium aspartate(MSA), 5 gm asparagine or placebo with 100 ml of $40^{\circ}$ whiskey by the 3 way-crossover design, while 12 volunteers of the other group were administered placebo, 1, 2 or 5 bottles of $Aspar^(circledR)$ containing 1 gm of MSA per bottle with 100 ml of $40^{\circ}$ whiskey by the 4 way-crossover design. Ethanol(and acetaldehyde) concentrations in venous blood drawn at 0, 0.25, 0.5, 1, 2, 4, 8th hour after ethanol ingestion were analysed by gas chromatogaphy. Subjective symptoms, liver function tests and psychomotor function tests were also performed during the study periods. Result; Plasma concentration and AUC of acetaldehyde in asparagine and MSA trials on ethanol ingestion were significantly lower than those of placebo trial in the 1st group. Plasma ethanol concentration of 5 bottle $Aspar^(circledR)$ trial was significantly lower than that of placebo trial in the 2nd group. Improvement of subjective symptoms or psychomotor performance by the treatment was not statistically significant. Conclusion; Aspartate and asparagine may be prospective candidates for acceleration of ethanol metabolism and prevention of ethanol toxicity.

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Polychlorinated biphenyls 중독 해독제의 개발에 관한 연구 (Studies on the Development of Detoxicating Agents of Polychlorinated biphenyls Toxicity)

  • 정기화;장판섭
    • 한국식품위생안전성학회지
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    • 제2권2호
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    • pp.51-56
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    • 1987
  • PCBs 투여로 중독된 rat에 대한 천문동, 황정, 백복령, 인삼, 오가피 등의 ethanol 추출물의 효과는 다음과 같다. 1. 생약추출물의 병용투여로 PCBs 중독으로 감소된 체중이 증가하였으나 간장중량의 현저한 회복효과는 볼 수 없었다. 2. 생약추출물을 병용투여함으로써 PCBs 중독으로 감소된 RBC, WBC, Hgb 및 Het의 값이 상승하였으며 특히 백복령과 인삼 추출물의 경우 회복효과가 컸다. 3. PCBs로 증가된 혈청중 cholesterol치와 total lipids가 생약추출물을 투여함으로써 감소되었다. 4. PCBs 투여로 증가된 혈청중 GOT, GPT 및 ALP의 활성도 생약추출물의 투여로 정상치에 가깝게 감소되었다.

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랫드에 있어 녹용 알콜 추출물의 TCDD-유발 고환 독성 방어 효과 (Ethanol Extract of Antler Velvet Attenuates Testicular Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD ) in Rats)

  • 최경운;황석연;김시관
    • 한국식품영양과학회지
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    • 제34권8호
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    • pp.1169-1174
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    • 2005
  • 본 연구는 다이옥신(2,3,7,8-tetrachlorodibenzo-P-dioxin, TCDD)에 노출된 랫드에 있어 녹용의 고환 독성에 대한 방어 효과를 조사하기 위하여 수행되었다. 실험은 30마리의 수컷 랫드를 10마리씩 3개군으로 나누어 정상대조군(CO)에는 vehicle을 TCDD-단독투여군(TA)과 녹용 에탄올 추출물(EAV) 투여군(TE)에는 50 $\mu$g/kg body weight의 다이옥신을 일회 복강내 주사하였다. TE는 다이옥신 노출 1주 전부터 녹용 에탄올 추출물을 20 mg/kg body weight씩 5주 동안 매일 복강 주사하였다 TA에서는 다이옥신에 의한 체중감소(CO 대비 61.1$\%$)가 현저하였으며 TE 역시 체중은 감소(CO 대비 77.0$\%$)하였으나 YA와 비교해 볼 때 그 정도는 유의하게 경감되 는 것을 알 수 있었다. 다이옥신(TCDD)에 의하여 야기되는 고환 중량, 정세관의 직경 및 Johnsen's score의 감소와 고환조직의 병변은 녹용 에탄올 추출물 투여에 의하여 유의하게 경감되었다. 이상의 결과로 보아 녹용은 랫드에 있어 다이옥신에 의하여 야기되는 고환 독성을 경감시킬 수 있다고 판단한다.