Browse > Article
http://dx.doi.org/10.15616/BSL.2021.27.3.161

Antioxidant and Anti-inflammatory Effects of Hot Water and Ethanol Extracts from Endemic Plants in Indonesia  

Hwang, Jin-Woo (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Choi, Jae-Ho (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Kang, Sang-Moon (R&D Center, ANPEP Inc.)
Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Publication Information
Biomedical Science Letters / v.27, no.3, 2021 , pp. 161-169 More about this Journal
Abstract
The present study, antioxidant and anti-inflammatory effects were measured of 6 endemic plants in Indonesian extracted by hot water or ethanol. The Nipa Fruticans Wurmb ethanol extract (NFWE) and Orthosiphon aristatus ethanol extract (OAE) showed the highest polyphenol and flavonoid contents of 203.70 and 33.70 ㎍/mg, respectively. Antioxidant activity of OAE was highest in DPPH radical scavenging activity (77.49% at 10 ㎍/mL) and ABTS+ radical scavenging activity (93.36% at 10 ㎍/mL). FRAP activity was significantly higher in NFWE than other extracts. Anti-inflammatory effects of 6 endemic plants in Indonesian extracted by hot water or ethanol were examined using nitric oxide (NO) inhibition assays. In the LPS-induced BV2 cells, OAE showed the highest inhibition of NO production without toxicity. The results of this study, suggest that OAE is a potential functional raw material for antioxidant and anti-inflammatory.
Keywords
Indonesia; Plants; Antioxidant; Anti-inflammatory; Cytotoxicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ishola IO, Awodele O, Olusayero AM, Ochieng CO. Mechanisms of analgesic and anti-inflammatory properties of Annona muricata Linn. (Annonaceae) fruit extract in rodents. J Med Food. 2014. 17: 1375-1382.   DOI
2 Kou X, Li B, Olayanju JB, Drake JM, Chen N. Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients. 2018. 10: 1-12.   DOI
3 Lee GW, Cho YH. Antioxidant Activity of Leaf Extract from Annona muricata. Review of Korea Contents Association. 2016. 14: 43-46.
4 Lin M, Zhang J, Chen X. Bioactive flavonoids in Moringa oleifera and their health-promoting properties. J Funct Foods. 2018. 47: 469-479.   DOI
5 Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeon BT. Antioxidant Activity and Protective Effects of Extracts from Helianthus tuberosus L. Leaves on t-BHP Induced Oxidative Stress in Chang Cell. J Korean Soc Food Sci Nutr. 2011. 40: 1525-1531.   DOI
6 Wang MY, Su C. Cancer preventive effect of Morinda citrifolia (noni). Ann NY Acad Sci. 2001. 952: 161-168.   DOI
7 Asare GA, Afriyie D, Ngala RA, et al. Antiproliferative activity of aqueous leaf extract of Annona muricata L. on the prostate, BPH-1 cells, and some target genes. Integr Cancer Ther. 2015. 14: 65-74.   DOI
8 Bae GS, Park SJ. The Anti-inflammatory Effect of Nypa fruticans Wurmb. Fruit on Lipopolysaccharide-induced Inflammatory response on RAW 264.7 cells. Kor J Herbol. 2016. 31: 79-84.
9 Sugai K, Watanabe S, Kuishi T, et al. Extremely low genetic diversity of the northern limit populations of Nypa fruticans (Arecaceae) on Iriomote Island. Japan Conserv Genet Resour. 2016. 17: 221-228.   DOI
10 Thang TD, Dai DN, Hoi TM, Ogunwande IA. Study on the volatile oil contents of Annona glabra L., Annona squamosa L., Annona muricata L. and Annona reticulata L., from Vietnam. Nat Prod Res. 2013. 27: 1232-1236.   DOI
11 Yosoff NA, Ahmad M, AI-Hindi B, Widyawati T, Yam MF, Mahmud R. Aqueous extract of Nypa fruticans Wurmb. Vinegar alleviates postprandial hyperglycemia in normoglycemic rats. Nutrients. 2015. 7: 7012-7026.   DOI
12 Zamora R, Vodovotz Y, Billiar TR. Inducible nitric oxide synthase and inflammatory diseases. Mol Med. 2000. 6: 347-373.   DOI
13 Lopez-Vidana EC, Figueroa IP, Cortes FB, Rojano BA, Ocana AN. Effect of temperature on antioxidant capacity during drying process of mortino (Vaccinium meridionale Swartz). Int J Food Prop. 2017. 20: 294-305.   DOI
14 Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem. 1912. 12: 239-243.   DOI
15 Torres MP, Rachagani S, Purohit V, et al. Graviola: a novel promising natural-derived drug that inhibits tumorigenicity and metastasis of pancreatic cancer cells in vitro and in vivo through altering cell metabolism, Cancer Lett. 2012. 323: 29-40.   DOI
16 Kwon YR, Youn KS. Antioxidant activity and physiological properties of Moringa (Moringa oleifera Lam.) leaves extracts with different solvents. Korean J Food Preserv. 2014. 21: 831-837.   DOI
17 Lee KW, Je H, Jung TH, et al. Comparison of components and antioxidant activity of cherry, aronia, and maquiberry. Korean J Food Nutr. 2018. 31: 729-736.   DOI
18 Lee YR. Antioxidant and α-Amylase Inhibitory Activity of 70% Ethanolic Extract from Morinda citrifolia L. (Noni). Korean J Food Nutr. 2020. 33: 210-214.   DOI
19 Pande M, Naiker M, Mills G, Singh N, Voro T. The Kura files: Qualitative social survey. Pac Health Surveill Response. 2005. 12: 85-93.
20 Moghadamtousi SZ, Karimian H, Rouhollahi E, Paydar M, Fadaeinasab M, Kadir HA. Annona muricata leaves induce G1 cell cycle arrest and apoptosis through mitochondriamediated pathway in human HCT-116 and HT-29 colon cancer cells. J Ethnopharmacol. 2014. 156: 277-289.   DOI
21 Prasad N, Yang B, Kong KW, et al. Phytochemicals and antioxidant capacity from Nypa fruticans Wurmb. Fruit. Evid Based Complement Alternat Med. 2013. 2013: 1-9.   DOI
22 Nawwar M, Ayoub N, Hussein S, et al. A flavonol triglycoside and investigation of the antioxidant and cell stimulating activities of Annona muricata Linn. Arch Pharm Res. 2012. 35: 761-767.   DOI
23 Nieva Moreno MI, Isla MI, Sampietro AR, et al. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol. 2000. 71: 109-114   DOI
24 Park SH, Kim JM, Kim JH, et al. Antioxidative Effects and Component Analysis of Graviola (Annona muricata) Leaf Extract/Fractions. J Soc Cosmet Sci Korea. 2017. 43: 309-320.   DOI
25 Hossain F, Islam A. Utilization of mangrove forest plant: Nipa palm (Nypa fruticans Wurmb.). Am J Agri Forest. 2015. 3: 156-160.   DOI
26 Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH. Antioxidant Activities of Extracts from Ligustrum ovalifolium H. Leaves. J Korean Soc Food Sci Nutr. 2011. 40: 1642-1647.   DOI
27 Matsushige A, Kotake Y, Matsunami K, Otsuka H, Ohta S, Takeda Y. Annonamine, a new aporphine alkaloid from the leaves of Annona muricata. Chem Pharm Bull (Tokyo). 2012. 60: 257-259.   DOI
28 Dole ckova I, Rarova L, Gruz J, Vondrusova M, Strnad M, Krystof V. Antiproliferative and antiangiogenic effects of flavone eupatorin, an active constituent of chloroform extract of Orthosiphon stamineus leaves. Fitoterapia. 2012. 83: 1000-1007.   DOI
29 Choi JH, Ohk SH. Evaluations on Antioxidant Effect of Water Extract from Graviola Leaves. J Korea Academia-Industrial cooperation Society. 2017. 18: 129-135.   DOI
30 Choi SS, Yim DS, Lee SK. Radical scavenging activities and protective effects against oxidative damage to DNA of extracts from medicinal plants with known osteoprotective effects. Korean J Pharmacogn, 2009. 40: 143-149.
31 Sreelatha S, Jeyachitra A, Padma PR. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem Toxicol. 2011. 49: 1270-1275.   DOI
32 Tamunaidu P, Saka S. Chemical characterization of various parts of nipa palm (Nypa fruticans). Ind Crop Prod. 2011. 34: 1423-1428.   DOI
33 Paul J, Gnanam R, Jayadeepa RM, Arul L. Anti-cancer activity on Graviola, an exciting medicinal plant extract vs various cancer cell lines and a detailed computational study on its potent anti-cancerous leads. Curr Top Med Chem. 2013. 13: 1666-1673.   DOI
34 Re R, Pellegrini N, Proteggente A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999. 26: 1231-1237.   DOI
35 Sun S, Liu J, Kadouh H, Sun X, Zhou K. Three new anti-proliferative Annonaceous acetogenins with mono-tetrahydrofuran ring from graviola fruit (Annona muricata). Bioorg Med Chem Lett. 2014. 24: 2773-2776.   DOI
36 Sunder J, Jeyakumar S, Sujatha T, Kundu A. Effect of feeding of morical: A herbal based supplement on production and egg quality in Japanese quail. Adv Anim Vet Sci. 2013. 1: 157-160.
37 Kang H. Anti-Oxidative and Anti-Neuroinflammatory Effect of Ethanol Extracts from Walnuts's (Juglans regia L.) Shell. Biomedical Science Letters. 2018. 24: 365-371.   DOI
38 Gopalakrishnan L, Doriya K, Kumar DS. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci Human Wellness. 2016. 5: 49-56.   DOI
39 Hamza AA. Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats. Food Chem Toxicol. 2009. 48: 345-355.   DOI
40 Hou YC, Janczuk A, Wang PG. Current trends in the development of nitric oxide donors. Curr Pharm Des. 1999. 5: 417-441.
41 Kim MS, Lee DC, Hong JE, et al. Antimicrobial Effects of Ethanol Extracts from Korean and Indonesian plants. Korean J Food Sci Technol. 2000. 32: 949-958.
42 Klungboonkrong V, Lamsal BP, Phoungchandang S. Changes and degradation kinetics of some bioactive compounds in dried Orthosiphon aristatus (Java tea) leaves during elevated temperature storage. J Sci Food Agric. 2019. 99: 933-940.   DOI
43 Ku KM, Kim SK, Kang YH. Antioxidant Activity and Functional Components of Corn Silk (Zea mays L.). Korean J Plant Res. 2009. 22: 323-329.
44 Choi JH, Hwang JW, Lee SG, Heo SH, Kang H. Antioxidant Effect of Hot Water Extracts from 3 Types Indonesia Plants (Hibiscus Petals, Moringa oleifera Gymnosperm, and Nipa Fruticans Wurmb). J Naturopath. 2021. 10: 42-47.   DOI
45 Tabrah FL, Eveleth BM. Evaluation of the effectiveness of ancient Hawaiian medicine. Hawaii Med J. 1966. 25: 223-230.
46 Adewole S, Ojewole J. Protective effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocintreated diabetic rats. Afr J Tradit Complement Altern Med. 2009. 6: 30-41.
47 Ameer OZ. Orthosiphon stamineus: traditional uses, phytochemistry, pharmacology, and toxicology: a review. J Med Food. 2012. 15: 678-690.   DOI
48 Dixon AR, McMillen H, Etkin NL. Ferment this: The transformation of noni, a traditional Polynesian medicine (Morinda citrifolia, Rubiaceae). Econ Bot. 1999. 53: 51-68.   DOI
49 Gavamukulya Y, Abou-Elella F, Wamunyokoli F, AEl-Shemy H. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pac J Trop Med. 2014. 7: 355-363.
50 Heinicke RM. The pharmacologically active ingredient of noni. Pac Trop Bot Gard Bull. 1985. 15: 10-14.