• 생약학회지
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• 제47권2호
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• pp.192-196
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• 2016

The herb of Agastache rugosa (Lamiaceae) called Korean mint as a spice or Agastache Herba as a crude drug is known to contain highly fragrant volatile substances. This research aimed to establish the quantitative gas chromatography (GC) method on the essential oil of A. rugosa using the three standard compounds, estragole, methyleugenol, pulegone, and to find whether the essential oil has anti-Alzheimer's activity. The GC quantification method was established by determining the linearity of calibration curve ($R^2$), linear range, and both limit-of-detection (LOD) and limit-of-quantification (LOQ). The $IC_{50}$ of the essential oil on the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were determined to be $69.06{\pm}0.26$ and $76.71{\pm}0.58{\mu}g/ml$, respectively.

• Natural Product Sciences
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• 제7권4호
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• pp.120-123
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• 2001

To develop systems for economic production of useful essential oil compounds, callus was induced from the seedlings of Agastache rugosa and cultured on MS medium. The volatile oil fraction was extracted from the callus and investigated by mean of GC-MS. The composition of the oil was compared with that of the mother plant. As a result, sixty five compounds including ferruginol were identified in the essential oil fraction. The main component of the oil from the leaves of Agastache rugosa was methyl chavichol (53.6%). Methyl jasmonate and jasmonic acid were added to the culturing cell suspension, separately and the composition of induced oil were compared. The oils from cultured cells treated with jasmonates showed considerably different patterns. Especially, the peak of estragole was found in callus oil after treatment with methyl jasmonate as though the amount was limited to 0.58%. In general, the TIC pattern of GC-MS of the callus oil became more similar to the oil from the leaves after elicitation.

• 한국약용작물학회지
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• 제10권3호
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• pp.206-211
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• 2002

박하와 배초향 정유의 보존료로서의 이용가능성을 타진하기 위해 그 성분조성을 GC-MSD 분석에 의해 실험한 결과 박하 정유의 주된 성분은 isomenthol로서 전체 정유성분 중 25.84%, menthol이 25.48%이었으며 배초향 정유에는 estragole이 79.83%, limonene이 0.12%함유되어 있었다. 2, 5, 10 mg 농도에서도. E. coli O157 : H7 ATCC 43895에 대한 박하 정유의 생육저해환은 $(9{\sim}14\;mm)$, 배초향 정유는 $(10{\sim}18\;mm)$, S. typhimurium ATCC 7988에 대해서는 박하 정유가 $(9{\sim}13\;mm)$, 배초향 정유가 $(13{\sim}20\;mm으로 나타나 두 정유성분 모두 농도 의존적으로 항균작용을 나타내었다. 생균수 측정 실험에서 박하 정유는 2 mg이하의 저농도에서 9시간까지 세균의 증식을 억제시켰으며 5 mg 이상의 농도에서는 9시간이후 E. coli O157 : H7 ATCC 43895와 S. typhimurium ATCC 7988을 모두 사멸시켰다. 배초향 정유는 2 mg이하의 농도에서 12시간까지 세균의 증식이 억제되었고 5 mg이상의 농도에서는 6시간이후 세균이 사멸되었다. E. coli O157 : H7 ATCC 43895에 대한 박하와 배초향 정유 성분의 항균작용을 전자현미경 촬영에 의해 확인한 결과 형태학적인 변화와 세포막과 세포벽의 분리 및 세포내용물 유실이 관찰되어 두 정유성분의 항균효과를 관찰할 수 있었다.

• 한국응용과학기술학회지
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• 제38권6호
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• pp.1370-1382
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• 2021

국내산 소재를 이용한 단일 추출물의 안전성 실험 및 화장품 소재로써의 선행 연구는 실시되어 왔지만, 개별 추출하여 식물의 특성을 극대화 및 효과 간섭 가능성에 대한 복합 혼합물에 대한 연구가 미비한 상황이다. 이에 본 연구는 배초향, 적송, 강황, 생강, 산미나리씨, 불수감에 대한 정유 추출에 따른 GC-MSD, 블렌딩 오일의 세포독성, 항산화, 항염을 확인하여 화장품 소재로서의 가능성을 확인하였다. GC-MSD 향기성분을 분석한 결과 주요 성분으로는 배초향은 Estragole, 적송은 à-Pinene, 강황과 생강은 Zingiberene, 산미나리씨 Anethole, 불수감 D-Limonene이 동정되었다. 세포독성이 확인되지 않은 100 uL/mL의 농도에서 NO 생성 70.62% 억제, DPPH 라디칼 소거능 64.03%, ABTS 라디칼 소거능 89.55%을 보였다. 이를 통하여 블렌딩 정유는 화장품 및 식품, 의약품 산업 분야에서 항산화 및 항염 효능이 있는 원료로서 유용하게 활용할 수 있는 가능성을 제시하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.57-57
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• 2020

본 연구는 국내 자생 방향성 식물의 자원발굴과 증식, DB 구축을 위하여 수행되었다. 배초향은 꿀풀과에 속하는 다년생 식물로 대표적인 한국의 방향식물이다. 한의학에서는 배초향의 지상부를 곽향이라 하여 음식으로 인한 체증, 오한, 복통 등을 치료하는 한약재로 사용되며 그 밖의 관상용, 밀원용, 식품용, 향신료 등 다양한 산업제품의 원료로 사용되고 있다. 배초향의 유전형질 특성 및 정유성분 조사를 위하여 국립농업유전자원센터로부터 배초향 종자(30자원)를 분양받아 발아시킨 후 시험연구포장에 증식하였다. 증식된 배초향을 대상으로 개화기, 식물 및 꽃의 색, 잎의 모양 등 개체별 형질특성을 조사하였으며 일정시기에 일시 수확하여 정유추출 후 GC/MS 분석을 통하여 정유의 화학성분을 분석하였다. 배초향의 개화시기는 6월 10일 경~7월 29일 경이었고 초장은 약 80cm~186cm이었다. 잎의 모양은 30자원 중 광난형 12자원, 피침형 10자원, 난형 8자원의 분포였고 식물체의 색은 green과 red-purple 계열이 각각 22자원과 8자원이었다. 배초향 꽃의 색깔은 RHS Colour chart 참조하여 149P 10P 8/4, 129B 10P 7/8, 37V 2.5RP 4/12의 색으로 분류하여 조사하였다. 129B 10P 7/8에 해당하는 자원이 18자원으로 가장 많은 분포를 차지하였다. 배초향의 정유성분 분석 결과, 정유수율은 0.11%~0.44%이었고 정유성분은 estragole, methyl eugenol, menthone 등이었다. 배초향의 주성분으로 알려진 estragole은 30자원 중 13자원의 주요정유성분인 것으로 나타났다.

• 한국식생활문화학회지
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• 제36권3호
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• pp.317-324
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• 2021

This study was carried out to investigate the flavoral essential oil components in the stems of Agastache rugosa. These components were analyzed using gas chromatography-mass selective detector (GC-MSD). The stems of Agastache rugosa were contained alcohols, aldehydes, ketones, fatty acid esters, and terpenoids. The peak area (%) of estragole was highest among its oil components and the next were pulegone and menthone. The terpenoid alcohols found were 1-octen-3-ol, chavicol, spatulenol, 3-hexen-1-ol, 2-cyclohexen-1-ol, methyl eugenol, and octaethyllene glycol. The stems also contained ketones such as pulegone, menthone, cis-isopulegone, 2-cyclohexene-1-one, 3-octanone, 1-cyclohexanone, isoindole-1-one, t-ionone, inden-2-one, as well as the aldehydes of 4-methoxycinnam and benzaldehyde. The following esters were also detected 1-isopulegone-3-yl acetate, caryophyllene oxide, acetate and benzendicarboxylic acid ester. The terpenoids in the stems were identified as caryophyllene, limonene, cyclohexasiloxane-D, germacrene-D, anethole, cadinene, muurolene, and bourbonene. Overall Agastache rugosa contained several functional oil components including phenylpropanoids and terpenoids as flavoral essential oil components for natural aromatherapy.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.610-616
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• 2002

The growth-inhibiting effects of Pinus densiflpora leaf-derived materials on nine human intestinal bacteria were investigated using the impregnated paper disk method, and their activities were compared with those of 13 commercially available terpenes. The biologically active constituent of the extract of P densiflora leaf was characterized as the monoterpene (1R)-(+)-$\alpha$-pinene by various spectroscopic analyses. Responses varied according to bacterial strain, chemicals, and dose. At 10 mg/disk, limonene and (1R)-(+)-$\alpha$-pinene strongly inhibited the growth of Clostridium perfringens, Staphylococcus aureus, and Escherichia coli, without adverse effects on the growth of five lactic acid-bacteria (Bifidobacterium adolescentis, B. bifidum, B. longum, Lactobacillus acidophilus, and L. casei). Little or no inhibition against seven bacteria was observed with anethole, borneol, camphor, caryophyllene, 1,8-cineole, estragole, linalool, and $\alpha$-terpineol. Structure-activity relationship revealed that (1R)-(+)-$\alpha$-pinene had more growth-inhibiting activity against C. perfringens than (1R)-(+)-$\beta$-, (1S-(-)-$\alpha$-, and (1S-(-)-$\beta$-pinenes. Furthermore, the growth-inhibition against L. casei was much more pronounced in (1R)-(+)-$\beta$- and (In-(-)-$\beta$-pinenes than (1R)-(+)-$\alpha$- and (1S)-(-)-$\alpha$-pinenes. These results indicate that the (+)-$\alpha$ form seems to be required against C. perfringens and $\beta$ form against L. casei for growth-inhibiting activity. Morphologically, most strains of C. perfringens were damaged and disappeared at 5 and 2 mg/disk of (1R)-(+)-$\alpha$-pinene. Morphological study revealed that (1R)-(+)-$\alpha$-pinene had more growth-inhibiting activity against C. perfringens than (1R)-(+)-$\beta$-, (1S)-(-)-$\alpha$-, and (1S)-(-)-$\beta$-pinenes. As naturally occurring growth-inhibiting agents, the Pinus leaf-derived materials described above could be useful preventive agents against diseases caused by harmful intestinal bacteria such as clostridia.

• 한국식품저장유통학회지
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• 제13권3호
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• pp.363-368
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• 2006

초임계추출법을 이용하여 향유로부터 정유물질을 추출하여 향기성분과 항산화물질을 최대로 추출할 수 있는 최적조건을 반응표면 분석법으로 얻고자 하였다. 추출물의 품질특성으로는 yield, total phenolics, electron donating ability 등을 분석하였으며 주요 향기성분으로는 estrgole과 L-carvone을 선정하였다. 반응변수들은 추출시간보다는 온도의 변화에 따라 비례적으로 증가하는 경향을 보였으며, 측정된 변수에 대하여 수립된 회귀식은 높은 결정계수값을 가지는 것으로 나타났다. 추출시간보다는 압력이 더 큰 변수로 작용하여 1% 이내의 유의성이 있었으며 이차 회귀식에 대한 적합성 결여분석 결과 수립된 반응표면 모형이 통계적으로 유의하였다. 능선분석을 통하여 각 변수에 대한 최적점과 추출조건을을 예측하였으며, 최적 추출조건은 실험구간내에서 추출물의 특성값을 최대로 할수 있는 조건을 제한조건하였을 때 최적조건은 238 bar, 42분이었다.

• Biomolecules & Therapeutics
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• 제20권1호
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• pp.113-117
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• 2012

The fruits of Foeniculum vulgare (Foeniculi Fructus) have been widely used in Chinese medicine as an antiemetic, ameliorating stomach ailments and as an analgesic. In order to establish its potential for antiallergic use, inhibitory actions of the fruit on 5-lipoxgenase (5-LOX) and ${\beta}$-hexosaminidase release were evaluated. The 70% ethanol extract of this plant material (FR) considerably inhibited 5-LOX-catalyzed leukotriene production from A23187-induced rat basophilic leukemia (RBL)-1 cells. The $IC_{50}$ was $3.2{\mu}g/ml$. From this extract, 12 major compounds including sabinene, fenchone, ${\gamma}$-terpinene, ${\alpha}$-pinene, limonene, p-anisylacetone, panisylaldehyde, estragole (4-allylanisole), trans-anethole, scopoletin, bergapten and umbelliferone were isolated. And it was found that several terpene derivatives including ${\gamma}$-terpinene and fenchone as well as phenylpropanoid, trans-anethole, showed considerable inhibitory action of 5-LOX. In particular, the $IC_{50}$ of trans-anethole was $51.6{\mu}M$. In contrast, FR and the isolated compounds did not show considerable inhibitory activity on the degranulation reaction of ${\beta}$-hexosaminidase release from antigen-treated RBL-2H3 cells. Against arachidonic acid-induced ear edema in mice, FR and trans-anethole showed significant inhibition by oral administration at doses of 100-400 mg/kg. In conclusion, FR and several major constituents are 5-LOX inhibitors and they may have potential for treating 5-LOX-related disorders.

• Food Science and Biotechnology
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• 제18권1호
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• pp.18-24
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• 2009

The volatile compounds of dried sancho (Zanthoxylum schinifolium), an aromatic plant were extracted by simultaneous distillation and extraction (SDE) method and identified by gas chromatograph-mass spectrometry (GC-MS). Selected chiral constituents of sancho oil were characterized by enantiodifferentiation using multidimensional gas chromatograph (MDGC)-MS. A total of 57 compounds were identified and quantified, and the major compounds were identified estragole, nonanoic acid, octanoic acid, $\beta$-phellandrenene, and limonene. Among them, estragol (63.9%) was found as the predominantly abundant component of sancho. $\alpha$-pinene and nerolidol, and $\beta$-pinene and linalool were determined to be enantiomerically pure (100%) for their (S)-form and (R)-form, respectively. The enantiomeric composition of limonene in sancho revealed 83.9% purity for the (S)-enantiomer, whereas (E)- and (Z)-rose oxides showed mixtures of both enantiomers. The enantiomeric excess (%) for citronellal was 22.6% with the (R)-enantiomer as major enantiomer. The enantiomeric composition of these compounds can be used as parameter for authenticity control of sancho.