• 대한수의학회지
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• 제20권2호
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• pp.159-165
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• 1980

A total of 98 strains of Bordetella bronckiseptica isolated from pigs affected the infectious atrophic rhinitis(AR) during 1978 were surveyed for drug sensitivity to 26 chemotherapeutic agents, and minimum inhibitory concentration(MIC), incidence rate of resistant strain and resistant patern from the strains which were obtained from the different pig farm in Jeonnam province were examined. The results obtained are summarized as follows. 1. Most of the strains tested were resistant to Ampicillin (AB, PC), spiramycin(SPO, sulfa drugs (SD) (MIC:$400.0{{\mu}g/ml}$) and streptomycin(SM) (MIC:$200.0{{\mu}g/ml}$). Of the 75.0% of strains were also resistant to penicillin(PC) (MIC:$200.0{{\mu}g/ml}$) and of the 14.3 of strains were inhibited to grow to tetracycline(TC), chlortetracycline(CTC), oxytetracyc-line(OTC), erythromycin(EM), tylosin(TS), leucomycin (LM) and chloramphenicol (CP) (MIC:$6.25{{\mu}g/ml}$). On the other hand, most of the strains tested were inhibited to grow to kanamycin(KM), gentamycin(GM) neomycin(NM) (MIC:$25.0{{\mu}g/ml}$) and to colistin(CL) (MIC:$12.5{{\mu}g/ml}$). 2. Incidence rate of resistant strains to main chemotherapeutic agents was 100.0% of sulfa drugs, 96.4% of streptomycin, 85.7% of penicillin, tetracycline, chloramphenicol and erythromycin, 46.4% of gentamycin, 17.9% of colistin and 0.0% of kanamycin and nalidixic acid.

• 대한임상검사과학회지
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• 제36권2호
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• pp.89-97
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• 2004

Many strains of Staphylococcus aureus were isolated from pus samples from primary, secondary, and tertiary medical institutions and were subjected to an antibiotic sensitivity test. Ciprofloxacin, clindamycin, erythromycin, gentamicin, oxacillin penicillin, tetracycline, trimethoprim/sulfamethoxazole, vancomycin and teicoplanin were used for the antibiotic sensitivity test. The strains showed hightest resistance to penicillin(91%), but all of strains tested were susceptible to vancomycin and teicoplanin. The isolated multi-drug(penicillin-tetracycline-ciprofloxacin-clindamycin-erythromycin- oxacillin-gentamicin) resistant S. aureus were analyzed genotypically using an AP-PCR(Arbitrarily Primed polymerase chain reaction) with an arbitrary 3 primers. Based on the result for genotype analysis, the genotypes identified by S1 primer did not coincide with those of S2 or E2 primers. Genotypes identified by S2 primer did not coincide with those of S1 or E2 primers. Also genotypes identified by the E2 primer did not coincide with those of S1 or S2 primers. Therefore, an analysis of AP-PCR test with multiple primers will provide more sensitive identification. A strain from a secondary medical institution and a strain from a tertiary medical institution which showed the same genotype for S1, S2, and E2 primers are required for further epidemiological study.

• 대한수의학회지
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• 제31권3호
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• pp.279-284
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• 1991

To investigate the epidemiological aspects of listeriosis, serotypes of L monocytogenes and antimicrobial susceptibilities of Listeria spp isolated from chicken carcases and chicken slaughter house environmental specimens were determined. Of 28 L monocytogenes strains, 12 strains(42.9%) were serotype 4, and the remaining 16 strains were untypable. Peak distributions of minimum inhibitory concentration$({\mu}g/ml)$ of the isolates were $0.78{\mu}g/ml$ for ampicillin, $0.39{\mu}g/ml$ for erythromycin and penicillin G, $1.56{\mu}g/ml$ for tetracycline and $6.25{\mu}g/ml$ or $12.5{\mu}g/ml$ for chloramphenicol, and $3.13{\mu}g/ml$ to > $100{\mu}g/ml$ for kanamycin and neomycin. Most of 214 isolates were sensitive to ampicillin and erythromycin, but 20. 1~78. 0% of the strains were resistant to tetracycline, kanamycin, penicillin-G and neomycin. Single or double drug resistance were observed in 75.8% of the resistant strains. The most common resistance patterns were Nm P-G(37.4%) in double pattern and P-G(23.7%) in single pattern.

• 대한수의학회지
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• 제45권1호
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• pp.75-83
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• 2005

The purpose of this study was to investigate the antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces. One hundred and forty-seven E. coli isolates showed resistance to tetracycline (95.2%), erythromycin (89.2%), ampicillin (70.1%), streptomycin (59.2%), cephalothin (56.5%), sulfamethoxazole/trimethoprim (53.7%), ciprofloxacin (57.1%), enrofloxacin (59.2%) and norfloxacin (57.1%). The multiple resistance was seen in 144 isolates (97.9%) and the rate of five, six and seven drugs resistance pattern were 20.4%, 18.4% and 16.3%, respectively. Also, the multiple resistance of E. coli to twelve drugs were seen in 1 isolates (0.7%). Fourteen Salmonella spp. showed resistance to ampicillin (50.0%), streptomycin (57.1%), erythromycin (64.3%) and tetracycline (57.1%) and the rate of two and three drugs resistance pattern were 4 isolates (28.6%), respectively. The prevalence of resistant organisms in Korea probably reflects lack of proper antibiotic policy resulting in prolonged and indiscriminate use of antimicrobial agents.

• 한국환경보건학회지
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• 제19권1호
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• pp.71-76
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• 1993

Nasal carrige of Staphylococcus was studied in relation to its significance as a source of the Staphylococci that caused hospital-acquired infection. Due to the trend of the increasing r esistance of S. aureus to many antimicrobial agents, it is necessary to study the sensitivity to antibiotics of this infectious microorganisms. 50 persons from general hospital and 50 college students were the object of this study. The following results were obtained 3 0 Strains of S. aureus were isolated. The rate of S. aureus nasal carrying were 26% in college students and 34% in hospital personnel. S. aureus which showed resistance to penicillin were 90%, tetracyclin 43%, erythromycin 37% and oxacillin 17%. The number of penicillin resistance of S. aureus were 11 (84%) in college students and 16 (94%) in hospital personnel. The number of strains of penicillin resistant S. aureus which produced 13-1actamase were 9 (82%) in college student and 14 (88%) in hospital personnel. Methicillin-resistant S. aureus (MRSA) which showed resistance to erythromycin and penicillin G were 100%, tetracyline, cephalothin and clindamycin were over 40% respectively, gentamicin 20%, SAM 20% and chloramphenicol 0%.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.417-422
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• 1985

Streptococcus faecalis var. liquefaciens에서 plasmid DNA를 분석한 결과, 4개의 plasmid를 가지고 있었으며, 각각의 대략적인 분자량은 6.8M-dal, 5.2Mdal, 2.6Mdal, 2.1Mdal로 측정되었다. 이 균주를 novobiocin으로 처리하여, 각각 다른 2개의 plasmid가 소실된 2개의 변이주를 얻었다. 이들 균주는 당발효성, 온도감수성, gelatin 용해성, 단백질응고성은 wild type과 동일하였으나 이중 한 균주가 lincomycin과 erythromycin에 대한 감수성을 나타내었다. 따라서 본 실험에서 검출한 4개의 plasmid는 당발효성 등의 특성과는 관련이 없는 것으로 추정할 수 있으며, pSK₂와 pSK₄의 두 plasmid 중 하나는 항생물질 저항성에 관련된 것으로 보인다.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.345-348
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• 1985

Mannitol hypertonic regeneration media를 사용하는 PEG-induced protoplast transformation system을 이용해서 pUB110과 pE194의 recombinant plasmid로 B. subtilis BR151을 transformation 시킴으로써 두 plasmid에서 유래되는 각각의 Neo$^{R}$와 Em$^{R}$을 동일한 recipient cell 내에서 동시에 발현시킬 수 있었다. Neomycin과 erythromycin을 함께 함유하는 mannitol regeneration media상에서 recombinant plasmid의 transformation frequency는 6.5 $\times$ $10^{-5}$이었다. 한편 transformant cell 내에서 recombinant plasmid의 replication이 agarose gel electrophoresis로 확인되었다.

• 대한미생물학회지
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• 제8권1호
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• pp.13-17
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• 1973

To understand the characteristics of 29 toxigenic strains of Corynebacterium diphtheriae isolated in Seoul area, type classification, biochemical properties and antibiotic susceptibility pattern to 9 kinds of antibiotics were investigated. The results obtained were summerized as follows; I. Among the 29 strains, gravis type was the overwhelming majority(24 strains), followed by intermedius type(3 strains) and mitis type(2 strains). II. Fermentation of glucose, maltose, lactose, trehalose and mannitol, nitrate reduction and urease were tested. All strains fermented glucose, but not sucrose, lactose, mannitol and trehalose. 9 strains fermented maltose and 20 strains did not. Nitrate was reduced by 28 strains but not by one strain. In urease test one strain showed positive, 28 strains negative. III. Antibiotic susceptibility test to penicillin G, chloramphenical, kanamycin, lincomycin, streptomycin, terramycin, erythromycin and gentamycin were carried out. The MIC of erythromycin(0.025 ${\mu}g/ml$ 26 strains and 0.05 ${\mu}g/ml$ 3 strains) was the lowest, followed by ampicillin, lincomycin and penicillin G. Streptomycin showed the highest MIC.

• 미생물학회지
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• 제55권3호
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• pp.293-295
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• 2019

메주로부터 분리한 Enterococcus faecalis DM01 균주는 8종의 항생제(ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, penicillin G, tetracycline, vancomycin) 저항성, 바이오필름 및 용혈현상을 나타내지 않았다. GC 함량 37.68%, 2,785,968 bp 크기의 단일 chromosome을 보유하고 있는 DM01 균주의 유전체 정보는 콩발효식품 제조용 종균 적용에 요구되는 항생물질 저항성, 바이오필름 및 용혈현상 관련의 안전성을 뒷받침하는 것으로 확인되었다.

• Advances in environmental research
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• 제7권3호
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• pp.201-212
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• 2018

In this study, conditions of solid phase extraction (SPE) for determination of some antibiotics such as trimethoprim, oxytetracycline, erythromycin, clarithromycin, azythromycin, doxycycline, sulfamethazine, ciprofloxacin, chlortetracycline, sulfamethoxazole in wastewaters were optimized. After the optimum volume and pH of the sample were determined, the effect of the concentration of the compounds and matrix were investigated. The highest recovery rates for antibiotic compounds were determined between 82% and 105% in 200 mL sample volume and pH 2.5. Then, antibiotic compounds were investigated in influent and effluent samples taken from Konya Urban Wastewater Treatment Plant. The concentration of the antibiotics was detected range of 0.11-101 ng/L in influent waters and