• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.194-201
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• 2017

This study was conducted to determine the valid time for accurate detection of complete blood cell count (CBC) in pig blood using an automatic blood corpuscle analyzer (Hemavet 950FS). Blood samples were collected from 34 pigs (Duroc) with a 60 kg (${\pm}3.5$) body weight. Ten specimens with CBC parameters in normal range and with no hemolysis were selected among 34 samples and used in this study. Regarding leukocytes parameters, white blood cell (WBC), neutrophil (NE), and lymphocyte (LY) counts showed a low daily variation (coefficient of variation, CV), whereas monocyte (MO), eosinophil (EO), and basophil (BA) CVs were significantly high (19.7, 56.9, and 53.3%, respectively). On the other hand, all parameters of erythrocytes and thrombocytes showed stable daily variation. All parameters of leukocytes and thrombocytes were significantly reduced as storage time passed (P<0.01 or 0.001), except for lymphocytes (P=0.535). However, no significant differences were observed in parameters of erythrocytes from blood up to 120 hours. From above results, we assert that Hemavet 950FS is useful in analyzing CBC, except for MO, EO, and BA. For accurate detection of leukocyte and thrombocyte parameters, analysis should be performed within 4 hours after blood collection when using Hemavet 950FS. On the other hand, parameters of erythrocytes could be stably detected for at least 120 hours after blood collection.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2791-2796
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• 2007

In their long journey through the cardiovascular circuit, erythrocytes are coerced to shape transform and assume different shapes on account of varying flow conditions in different blood vessels. The present work was aimed to visualize these erythrocyte shape transformations by an invitro microcirculatory model, and assess multi-shape erythrocyte deformability. The model uses an in-house fabricated, inexpensive disposable micro flow channel to mimic certain invivo conditions and a fast frame video microscopic system for imaging the shape changes in erythrocytes. Results show the multi-shape transformation of erythrocyte christened as discoidal shape, the asymmetrically deformed 'hat' and 'bullet-like' shapes, and the axially deformed 'slipper' and 'spindle-like' shapes. Specific erythrocyte showed the shape transition and transformation while passing through the observed window. The obtained erythrocyte shapes very analyzed for deformability index using image processing techniques that varied significantly (p <0.001) for different shapes as compared with the resting shape.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.29-33
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• 1990

The study was attempted to scrutinize the normal osmotic fragility of erythrocyte in the domestic ruminants, making a comparison with that of human and canine. The animals used in the experiment were apparently healthy adult Holstein cattle, Korean native cattle, sheep (Corriedale) and Korean native goats. Osmotic fragility of erythrocytes was the highest in the Korean native goats, and the next were sheep and Korean native cattle, and Holstein cattle in order. In other words, erythrocytic resistance to osmotic lysis was the strongest in Holstein cattle and the most weak in Korean native goats among the domestic ruminants. In general, resistance of erythrocytes was stronger in human and canine than in the domestic ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.6
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• pp.671-677
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• 1996

Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination (MSHA) and mannose-resistant hemagglutination (MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-labile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28 (3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable (STaI and STaII) and heat-labile (LT) enterotoxin genes in E. coli.

• Environmental Mutagens and Carcinogens
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• v.18 no.2
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• pp.129-134
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• 1998

To evaluate the genotoxicity of CKD-602, an anticancer agent the in viかo reverse mutation assay using Salmonella typhimurium, the Chromosome aberration assay using Chinese hamster lung (CHL) cells and the in vivo micronucleus assay using bone marrow cells of ddY mice were performed. In the reverse mutation assay, CKD-602 did not induced mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535, and TA 1537 strains with and without metabolic activation. In the chromosome aberration test using CHL cells, there was an increased incidence of structural aberrations induced by CKD-602 without metabolic activation during 24 and 48 hours, but CKD-602 did not induce chromosome aberration with metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ddY mice. At 24 hours after treatment with CED-602 by i.p. once, there was an increased incidence of micronucleated polychromatic erythrocytes in bone marrow of ddY male mice.

• Environmental Mutagens and Carcinogens
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• v.18 no.2
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• pp.123-128
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• 1998

To evaluate the genotoxicity of SKP-450, an antihypertensive agent the in vitro reverse mutation assay using Salmonella typhimurium, the Chromosome aberration assay using Chinese hamster lung (CHL) cells and the in vivo micronucleus assay using bone marrow cells of ddY mice were performed. In the Reverse mutation test, SKP-450 did not induced mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 with and without metabolic activation. In the chromosome aberration assay using CHL cells, there was no increased incidence of structural and numerical aberrations with and without metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ddY mice at 30 hours after treatment with SKP-450 by p.o once. The results showed no increased incidence of micronucleated polychromatic erythrocytes in bone marrow of ddY male mice treated with SKP-450.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.57-63
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• 1997

Animal and clinical investigations have shown that fluoroquinolones, new quinolone antibacterial agents (NQs), are well absorbed across the intestinal tract, with a bioavailability of 60-90% after oral administration. Although some types of carrier-mediated intestinal transport mechanisms have been reported for enoxacin (ENX), ofloxacin (OFLX) and sparfloxacin (SPFX), recent results using a human intestinal epithelial cell line, Caco-2, indicated a passive or nonsaturable transport of SPFX, one of the most hydrophobic NQs. The mechanism underlying the intestinal absorption of NQs is still largely unknown. The distribution of NQs into peripheral tissues including erythrocytes is very rapid and their tissue-to-plasma concentration ratios (Kp) are considerably larger than those of inulin (an extracellular fluid space marker), in spite of almost complete ionization of NQs at the physiological pH. Our findings suggest that OFLX and lomefloxacin (LFLX) are taken up by rat erythrocytes via a transport system common to that of a water-soluble vitamin, nicotinic acid.

• The Korean Journal of Pharmacology
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• v.4 no.1 s.5
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• pp.9-16
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• 1968

The author studied the hemolytic action of phenothiazine derivatives such as promazine, prochlorper azine, perphenazine and thiethylperazine on rabbit erythrocytes, and obtained the following results: 1. Promazine, prochlorperazine, perphenazine and thiethylperazine caused hemolysis in vitro in the following order: Thiethylperazine>perphenazine>prochlorperazine>promazine. 2. Cholesterol inhibited the hemolytic action of prochlorperazine, perphenazine and thiethylperazine, but had no effect on promazine hemolysis. 3. Dextrose, albumin and blood plasma protected erythrocytes against promazine, prochlorperazine, perphenazine and thiethylperazine. 4. The intravenous injection of promazine, prochlorperazine, perphenazine and thiethylperazine caused hemolysis in the same order as they did in vitro.

• Korean Journal of Pharmacognosy
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• v.42 no.1
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• pp.68-75
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• 2011

A new lectin was purified from Bombyx mori (BML) by physiological saline extraction, ammonium sulfate precipitants, anion exchange column chromatography on DEAE Sephadex A-50 and gel filtration column chromatography on Sephadex G-200. BML agglutinated trypsinized and glutaraldehyde-fixed erythrocytes, and was observed the most high activity with rabbit, chicken erythrocytes and rat splenic lymphocytes. Agglutinability was markedly affected at highly acidic pH, but was relatively stable with high temperature. The effect of metal ions was observed and BML was affected by bivalaent cations, especially depending on $Ca^{2+}$, $Fe^{2+}$, $Mn^{2+}$, whereas, inhibited by $Mg^{2+}$. Agglutination was strongly inhibited by heparin and glucuronic acid. BML was proved to be a glycoprotein which contains 17.16% of sugars. By mass spectrometry analysis, we found 2 bands that were considered as lectin subunits.

• Korean Journal of Veterinary Research
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• v.31 no.4
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• pp.381-387
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• 1991

Deer hemal nodes were studied by gross anatomy and light microscopy. Hemal nodes were scattered in the periphery of thoracic and abdominal aortae, and spherical or avoid in shape. A hemal node consisted of a thin capsule and a hilum, and had numerous small subcapsular and deep sinuses distended by a great number of erythrocytes. Although a few lymphatic nodules and tissues were seen in the parenchyma, no typical cortex and medulla was defined. Blood vessels occurred, but lymph vessel was not observed in nodes. The parenchyma of the hemal node consisted of many erythrocytes and lymphocytes, and a few granulocytes, plasma cells megakaryocytes and macrophages. The stroma was composed of reticular cells and fibers. The capsule and trabecula was a collagenous connective tissue with smooth myofibers. The above findings suggest that the hemal nodes are involved in blood storage, blood filtration and immune reaction.