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http://dx.doi.org/10.5762/KAIS.2017.18.1.194

Evaluation of valid time for analysis of complete blood cell in pig blood using the Hemavet 950FS  

Kim, Ki-Hyun (National Institute of Animal Science, Rural Development Administration)
Kim, Kwang-Sik (National Institute of Animal Science, Rural Development Administration)
Kim, Doo-Wan (National Institute of Animal Science, Rural Development Administration)
Sa, Soo-Jin (Technology Cooperation Bureau, Rural Development Administration)
Kim, Young-Hwa (National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.18, no.1, 2017 , pp. 194-201 More about this Journal
Abstract
This study was conducted to determine the valid time for accurate detection of complete blood cell count (CBC) in pig blood using an automatic blood corpuscle analyzer (Hemavet 950FS). Blood samples were collected from 34 pigs (Duroc) with a 60 kg (${\pm}3.5$) body weight. Ten specimens with CBC parameters in normal range and with no hemolysis were selected among 34 samples and used in this study. Regarding leukocytes parameters, white blood cell (WBC), neutrophil (NE), and lymphocyte (LY) counts showed a low daily variation (coefficient of variation, CV), whereas monocyte (MO), eosinophil (EO), and basophil (BA) CVs were significantly high (19.7, 56.9, and 53.3%, respectively). On the other hand, all parameters of erythrocytes and thrombocytes showed stable daily variation. All parameters of leukocytes and thrombocytes were significantly reduced as storage time passed (P<0.01 or 0.001), except for lymphocytes (P=0.535). However, no significant differences were observed in parameters of erythrocytes from blood up to 120 hours. From above results, we assert that Hemavet 950FS is useful in analyzing CBC, except for MO, EO, and BA. For accurate detection of leukocyte and thrombocyte parameters, analysis should be performed within 4 hours after blood collection when using Hemavet 950FS. On the other hand, parameters of erythrocytes could be stably detected for at least 120 hours after blood collection.
Keywords
Blood corpuscle analyzer; Erythrocytes; Leukocytes; Pig; Thrombocytes;
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1 K. R. Park, Y. C. Cho, The Association of Blood Pressures and Blood Biochemical Properties with BMI in Health Checkup Examinees. J. Korea Acad. Industr. Coop. Soc., 12, 3072-3081, 2011. DOI: http://dx.doi.org/10.5762/KAIS.2011.12.7.3072   DOI
2 K. J. Moon, H. Y. Kim, Effects of brown rice-vegetable school meal program on subjective health status, BMI and hematological parameters among high school students. J. Korea Acad. Industr. Coop. Soc., 16, 7385-7393, 2015. DOI: http://dx.doi.org/10.5762/KAIS.2015.16.11.7385   DOI
3 M. A. Lichtman, E. Beutler, T. J. Kipps, U. Seligsohn, K. Kaushansky, J. T. Prchal, 'Williams Hematology', 7th Ed., McGraw-Hill, New York, 2006.
4 M. A. Islam, C. Grobe-Brinkhaus, M. J. Proll, M. J. Uddin, S. A. Rony, D. Tesfaye, E. Tholen, M. Holker, K. Schellander, C. Neuhoff, Deciphering transcriptome profiles of peripheral blood mononuclear cells in response to PRRSV vaccination in pigs. BMC Genomics, 17, 641-658, 2016. DOI: http://dx.doi.org/10.1186/s12864-016-2849-1   DOI
5 P. Dubreuil, Y. Couture, C. Farmer, D. Petitclerc, Hematological and biochemical changes following an acute stress in control and somatostatin-immunized pigs. Can. J. Anim. Sci., 73, 241-252, 1993. DOI: http://dx.doi.org/10.4141/cjas93-026   DOI
6 E. G. Welles, A. S. Hall, D. M. Carpenter, Canine complete blood counts: a comparison of four in-office instruments with the ADVIA 120 and manual differential counts. Vet. Clin. Pathol., 38, 20-29, 2009. DOI: http://dx.doi.org/10.1111/j.1939-165X.2008.00084.x   DOI
7 D. B. Duncan, Multiple range and multiple F tests. Biometrics. 11, 1-42, 1955. DOI: http://dx.doi.org/10.2307/3001478   DOI
8 S. L. Putwain, E. J. Villiers, J. Archer, Validation of the Hemavet HV950FS multispecies haematology analyser for analysis of canine blood. Comp. Clin. Pathol., 17, 1-8,2008. DOI: http://dx.doi.org/10.1007/s00580-007-0708-9
9 U. Windberger, A. Bartholovitsch, R. Plasenzotti, K. J. Korak, G. Heinze, Whole blood viscosity, plasma viscosity and erythrocyte aggregation in nine mammalian species: reference values and comparison of data. Exp. Physiol., 88, 431-440. 2003. DOI: http://dx.doi.org/10.1113/eph8802496   DOI
10 Y. Nishiyama, H. Niiyama, H. Harada, A. Katou, N. Yoshida, H. Ikeda, Effect of Exercise Training on Red Blood Cell Distribution Width as a Marker of Impaired Exercise Tolerance in Patients With Coronary Artery Disease. Int. Heart J. DOI: http://dx.doi.org/10.1536/ihj.16-015
11 M. J. Jo, J. K. Ma, A. R. Shin, F. Dong, K. B. Song, Y. H. Choi, H. K. Lee, Relationship between periodontal status and blood count and anemia in adults: Based on the 5th Korea National Health and Nutrition Examination Survey. J. Korean Acad. Oral Health, 39, 152-157, 2015. DOI: http://dx.doi.org/10.11149/jkaoh.2015.39.2.152   DOI
12 J. T. Latham, J. R. Bove, F. L. Weirich, Chemical and Hematologic Changes in Stored Cpda-1 Blood. Transfusion, 22(2), 158-159, 1982. DOI: http://dx.doi.org/10.1046/j.1537-2995.1982.22282177126.x   DOI
13 K. R. Park, Y. C. Cho, The abnormal rates of blood pressures and blood biochemical properties with BMI in health checkup examinees. J. Korea Acad. Industr. Coop. Soc., 11, 4843-4853, 2010. DOI: http://dx.doi.org/10.5762/KAIS.2010.11.12.4843   DOI
14 M. F. Burks, M. E. Tumbelson, K. W. Hicklin, D. P. Hutcheson, C. C. Middleton, Age and sex related changes of hematologic parameters in Sinclair (S-1) miniature swine. Growth, 4l, 5l-52, 1977.
15 T. B. Klem, E. Bleken H. Morberg, S. I. Thoresen, T. Framstad, Hematologic and biochemical reference intervals for Norwegian crossbreed grower pigs. Vet. Clin. Pathol., 39, 221-226, 2010. DOI: http://dx.doi.org/10.1111/j.1939-165X.2009.00199.x
16 J. P. Aubuchon, T. N. Estep, R. J. Davey, The Effect of the Plasticizer Di-2-Ethylhexyl Phthalate on the Survival of Stored Rbcs. Blood, 71(2), 448-452, 1988.
17 N. Robinson, A. Kirchbichler, O. Banuls, M. Mader, R. Aikin, P. E. Sottas, G. D'Onofrio, Validation of a Blood Stability Score as an easy-to-use blood sample quality index. Int. J. Lab. Hematol., 2016. DOI: http://dx.doi.org/10.1111/ijlh.12557
18 U. Salzer, R. Zhu, M. Luten, H. Isobe, V. Pastushenko, T. Perkmann, P. Hinterdorfer, Giel J.C.G.M. Bosman, Vesicles generated during storage of red cells are rich in the lipid raft marker stomatin. Bosmanet, Transfusion, 48(3), 451-462, 2008. DOI: http://dx.doi.org/10.1111/j.1537-2995.2007.01549.x   DOI
19 T. J. Greenwalt, C. G. McGuinness, U. J. Dumaswala, Studies in Red-Blood-Cell Preservation .4. Plasma Vesicle Hemoglobin Exceeds Free Hemoglobin. Vox Sang, 61(1),14-17, 1991. DOI: http://dx.doi.org/10.1111/j.1423-0410.1991.tb00920.x   DOI