Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Biochemical Properties of the Lectin Isolated from Bombyx mori

누에(Bombyx mori)로부터 분리한 렉틴의 생화학적 특성

  • Kim, Se-Jin (Dept. Biology, College of Sciences, Yeungnam University) ;
  • Lee, Sang-Yong (Dept. Biology, College of Sciences, Yeungnam University) ;
  • Jeune, Kyung-Hee (Dept. Biology, College of Sciences, Yeungnam University)
  • Received : 2010.12.06
  • Accepted : 2010.12.27
  • Published : 2011.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

A new lectin was purified from Bombyx mori (BML) by physiological saline extraction, ammonium sulfate precipitants, anion exchange column chromatography on DEAE Sephadex A-50 and gel filtration column chromatography on Sephadex G-200. BML agglutinated trypsinized and glutaraldehyde-fixed erythrocytes, and was observed the most high activity with rabbit, chicken erythrocytes and rat splenic lymphocytes. Agglutinability was markedly affected at highly acidic pH, but was relatively stable with high temperature. The effect of metal ions was observed and BML was affected by bivalaent cations, especially depending on $Ca^{2+}$, $Fe^{2+}$, $Mn^{2+}$, whereas, inhibited by $Mg^{2+}$. Agglutination was strongly inhibited by heparin and glucuronic acid. BML was proved to be a glycoprotein which contains 17.16% of sugars. By mass spectrometry analysis, we found 2 bands that were considered as lectin subunits.

Keywords

References

  1. Goldstein, I. J., Hughes, R. C., Monsigny, M., Osawa, T. and Sharon, N. (1980) What should be called a lectin? Nature 285: 66.
  2. Chung, S. R. and Jeune, K. H. (1986) Lectins. Review of Biochem. Biochem. Soc. Kor. 1: 371-382.
  3. Ingram, G. A. and Molyneux, D. H. (1991) Insect lectins: Role in parasite-vector interactions. In Kilpatrick, D. C., Van Driessche, E. and Bog-Hansen, T. C. (eds.) Lectin Reviews Vol. 1, 103-127, Sigma Chemical Company, St. Louis, MO, USA.
  4. Kim, J. H. and Lee, I. H (2003) 곤충의 생체방어 단백질로서의 lectin. BioWave 5(6): 1-8.
  5. Suzuki, T. and Natori, S. (1983) Identification of a protein having hemagglutinating activity in the hemoymph of the silkworm, Bombyx mori. J. Biochem. 93: 583-590.
  6. Amanai, K., Sakurai, S. and Ohtaki, T. (1990) Purification and characterization of hemagglutinin in the haemolymph of the silkworm, Bombyx mori. Comp. Biochem. Physiol. 97B: 471-476.
  7. Amanai, K. Sakai, M., Sakurai, S., Mori, T., Nikaido, O. and Ohtaki, T. (1991) Occurrence of lectin in the silkgland of the silkworm, Bombyx mori. Develop. Growth & Differ. 33(4): 421-427. https://doi.org/10.1111/j.1440-169X.1991.421_a.x
  8. Koizumi, N., Imamura, M., Kadotani, T., Yaoi, K., Iwahana, H. and Sato, R. (1999) The lipopolysaccharide-binding protein participating in hemocyte nodule formation in the silkworm Bombyx mori is a novel member of the C-type lectin superfamily with two different tandem carbohydrate-recognition domains. FEBS Letters 443: 139-143. https://doi.org/10.1016/S0014-5793(98)01701-3
  9. Ochiai, M. and Ashida, M. (1988) Purification of a ${\beta}-1$,3-Glucan recogmition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori. J. Biol. Chem. 263(24): 12056-12062.
  10. Yoshida, H., Kinoshita, K. and Ashida, M. (1996) Purification of a peptidoglycan recognition protein from hemolymph of the silkworm, Bombyx mori. J. Biol. Chem. 271(23): 13854-13860. https://doi.org/10.1074/jbc.271.23.13854
  11. Ochiai, M. and Ashida, M. (2000) A pattern-recognition protein for ${\beta}-1$,3-Glucan. J. Biol. Chem. 275(7): 4995-5002. https://doi.org/10.1074/jbc.275.7.4995
  12. Lis, H. and Sharon, N. (1986) Biological properties of lectins. In Liener, I.E., Sharon, N. and Goldstein, I. J. (eds.), The Lectins, 266, Academic Press, New York.
  13. Jeune, K. H., Jung, M. Y., Choi, S. J., Lee, J. W., Park, W. H., Cho, S. H., Lee, S. H. and Chung, S. R. (2001) Immunomodulating effect of the lectin from Allomyrina dichotoma. Kor. J. Pharmacogn. 32(1): 31-38.
  14. Jeune, K. H., Kim, S. J. and Chung, S. R. (2005) Cytokine expressions with lectins from Allomyrina dichotoma and Bombyx mori. Kor. J. Pharmacogn. 36(2): 129-135.
  15. Jo, S. H., Kim, S. J., Chung, S. R. and Jeune, K. H (2006) Effect of lectin isolated from Serrognathus platymelus castanicolor lavae on the various cytokine expressions. Kor. J. Pharmacogn. 37(4): 221-228.
  16. Nowak, T. P., Kobilae, D., Rael, L. E. and Barondes, S. H. (1977) Developmentally regulated lectin from embryonic chick pectoral muscle. J. Biol. Chem. 252: 6026-6030.
  17. Chung, S. R., Jeune, K. H. and Kim, K. A. (1980) Isolation, purification and characterization of phytohemagglutinating proteins from Korean natural products. Arch. Pharm. Res. 3: 31-36. https://doi.org/10.1007/BF02884758
  18. Chung, S. R. Hong, S. S. and Jeune-Chung, K. H. (1983) Isolation, purification of lectin from Phaseolus radiatus. Yakhak Hoeji 27: 221-227.
  19. Iglesias, J. L., Lis, H. and Sharon, N. (1982) Purification and properties of a D-galactose/N-acetyl-D-galactosamine specific lectin from Erythrina cristagalli. Eur. J. Biochem. 123: 247-252. https://doi.org/10.1111/j.1432-1033.1982.tb19760.x
  20. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, J. (1951) Protein measurement with folin phemol reagent. J. Biol. Chem. 193: 265.
  21. Moon, I. J. (1995) Biochemical characteristics, lymphocytes mitogenicity and cancer cell agglutinability of the lectin from Lentinus edodes. Ph. D. Thesis, 1-88, Yeungnam university.
  22. Laemmli, G. U. K. and King, J. (1970) Polypeptides of the tail fibers of bateriophage T4. J. Mol. Biol. 62: 465-477.
  23. Sitohy, M., Doheim, M. and Bahr, H. (2007) Isolation and characterization of a lectin with antifungal activity from Egyptian Pisum sativum seeds. Food Chemistry 4(3): 971-979.
  24. 박나영, 이삼빈, 노광수 (2007) Biochmical charaterization of lectin isolated from cherry tomato fruit. J. Life Science 17(2): 254-259. https://doi.org/10.5352/JLS.2007.17.2.254
  25. Kim, M. K. (1990) Studies on lectins from Korean higher fungi - Isolation, purification and biochemical properties of lectins from Lactarius piperatus. Ph. D. Thesis, 1-79, Yeungnam university.
  26. Feng, K., Liu, Q. H., Ng, T. B., Liu, H. Z., Li, J. Q., Chen, G., Sheng, H. Y., Xie, Z. L. and Wang, H. X. (2006) Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens. Biochem. Biophys. Res. Commun. 345: 1573-1578. https://doi.org/10.1016/j.bbrc.2006.05.061
  27. Cheung, A. H. K., Wong, J. H. and Ng, T. B. (2009) Musa acuminata (Del Monte banana) lectin is a fructose-binding lectin with cytokine-inducing activity. Phytomedicine 16: 594-600. https://doi.org/10.1016/j.phymed.2008.12.016
  28. Sun, J., Wang, L., Wang, B., Guo, Z., Liu, M., Jiang, K., Tao, R. and Zhang, G. (2008) Purification and characterization of a natural lectin from the plasma of the shrimp Fenneropenaeus chinensis. Fish & Shellfish Immunology 25: 290-297. https://doi.org/10.1016/j.fsi.2008.06.001
  29. Rittidach, W., Paijit, N. and Utarabhand, P. (2007) Purification and characterization of a lectin from the banana shrimp Fenneropenaeus merguiensis hemolymph. Biochim. Biophys. Acta 1770: 106-114. https://doi.org/10.1016/j.bbagen.2006.06.016
  30. Park, C. S. (1995) Cancer cell agglutinability and biochemical properties of the lectins from starfish, Asterina pectinifera. Ph. D. Thesis, 1-88, Yeungnam university.
  31. KuKu, A. and Eretan, O. B. (2004) Purification and partial characterization of a lectin from the fresh leaves of Kalanchoe crenata (Andr.) How. J. Biochem. Mol. Biol. 37: 229-233. https://doi.org/10.5483/BMBRep.2004.37.2.229
  32. Nho, S. K and Lee, J. M. (2000) Characteristics of Korean native strains in the domesticated silkworm, Bombyx mori. Korean J. Seric. Sci. 42(1): 10-13.
  33. Kitagaki, H., Iida, N., Matsumoto, I. and Seno, N. (1986) Carbohydrate-binding specificity of silkworm lectin. Carbohydrate Research 151: 271-278.
  34. Kilpatrick, D. C. (1991) Alphabetical directory of animal lectins. In kilpatrick, D.C.(eds.) Handbook of animal lectins: Properties and biomedical applications. 79-4567, John Wiley & Sons, Ltd.
  35. Yun, E. Y, Kang, S. W., Hwang, J. S., Goo, T. W., Kim, S. H., Jin, B. R., Kwon, O. Y and Kim, K. Y. (1999) Moecular clonig and characterization of a cDNA encoding a tansferrin homolog from Bombyx mori. Biol. Chem. 380: 1455-1459.
  36. Yun, E. Y, Lee, J. K., Kwon, O. Y, Hwang, J. S., Kim, I. S., Kang, S. W., Lee, W. J., Ding, J. D., You, K. H. and Goo, T. W. (2009) Bombyx mori transferrin: Genomic structure, expression and antimicrobal activity of recombinant protein. Dev. Comp. Immunol. 33: 1064-1069. https://doi.org/10.1016/j.dci.2009.05.008