• 한국식품영양과학회지
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• 제5권1호
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• pp.1-9
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• 1976

The variations of growth gain and the composition of free amino acid levels in plasma and erythrocytes of young rats(wistar strain male) were determined by microbioassay method, feeding diets of rice group and 7% casein group as a control for three weeks. The results were as follows; 1. The growth gain of control diet group was higher than the rice diet group. 2. The contents of free tryptophan, lysine, and threonine levels in plasma and erythrocytes on rats of 7% casein group were higher than the rice group. 3. In the 7% casein diet group and the rice group, these free amino acids were included more in erythrocytes than in plasma. 4. Therefore, generally feeding by high protein score diet was included more Try, Lys, Thr in plasma and erythrocytes than feeding by low protein score diet. So the high and low of protein score was assumed by the contents of Try, Lys, Thr in plasma and erythrocytes on rats.

• 한국대기환경학회지
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• 제6권1호
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• pp.111-117
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• 1990

To investigate the transplacental cytogenic effect of air pollutants the authors collected samples from Shinchon, Guro, Banpo and Jungnung-dongs in winter season. The air filters were extracted by mixture of benzene and ethanol, then a certain amount of extracted sustance was injected to pregnant mice at 16th day of gestation. From the fetal liver emulsion polychromatic erythrocytes were collected and stained with Giemsa solution. The cytogenic effect was evaluated by micronucleus test by which numbers of polychromatic erythrocytes containing microunclei (MNPCE) per 1, 000 polychromatic erythrocytes could be counted.

• 대한수의학회지
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• 제10권1호
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• pp.11-23
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• 1970

Recently Carter(1952) reported the capsule antigens of Pasteurella multocida could be divided into four serological types A,B,C and D by means of precipitation tests. Subsequently he showed that the most sensitive for identification of these types involved the use of capsule substance adsorbed by erythrocytes in hemagglutination test. It may be somewhat difficult to conduct the hemagglutination test in small laboratory, because relatively large amounts of antisera and erythrocytes of the human O type are required for the test. A simple method for serological typing of P. multocida was the slide agglutination test employed by Little et al. (1943) and Namioka et al. (1962), but this method is still in controversy. The author tried adapting Carter's hemagglutination method to the slide method so called "micromethod technique", and studied on the stabilization of erythrocytes for use of slide hemagglutination to P. multocida although many invesigators reported the stabilization of erythrocytes. The results obtained are summarized as follows: 1. A simplified method (slide method) for capsule typing of the organism was developed by adapting Carter's hemagglutination reaction(tube method). Antibody-containing serum can be diluted serially on Boerner's microtest slide with capillary or serological pipetts with a considerable accuracy. The slide reaction can be carried out with case on the slide by adding $0.05m{\ell}$ of antigen-sensitized erythrocytes suspension diluted to one percent on $0.05m{\ell}$ of serially diluted antibody-containing sera, and the final result can be read after 60 minutes at the room temperature ($15^{\circ}C$). 2. It is difficult to determine superiority of inferiority between the slide method and the tube method on the pattern of the reaction of hemagglutination. 3. The pH range of 6.6 to 8.3 is optimal for the slide hemagglutination reaction. 4. The antigen-sensitization against erythrocytes at $37^{\circ}C$ is optimal for the slide hemagglutination. 5. Both the doses and concentration of antigen do not influence the antigen-adsorbing capacity of erythrocytes. 6. The reduction of antigen-sensitizing hours does not influence the antigen-adsorbing capacity of erythrocytes even 30 minutes. 7. The tannic acid treatment against formalinized and non-formalinized erythrocytes showed no effect on the reaction of hemagglutination. 8. The erythrocytes preserved at $4^{\circ}C$ in the ACD solution do not decrease the reactivity on the reaction of hemagglutination for 60 days, while they begin slight hemolysis 30 days after preserving. 9. The stable preparation of erythrocytes can be obtained by treating the cells at $37^{\circ}C$ for 20 hours with from 4 to 8 percent of formalin in saline or buffer. These cells can be preserved at $4^{\circ}C$ for more than 8 months experimented without hemolysis. With low concentration of formalin, the cells were not sufficiently stabilized resulting in the hemolysis after short period of preservation at $4^{\circ}C$. 10. The erythrocytes treated with 16 percent of formalin remain constantly or increase the reactivity for the reaction of hemagglutination. On the contrary, the cells treated with I to 8 percent of formalin decrease the reactivity. 11. There is no difference between nontreated fresh erythrocytes and the erythrocytes preserved in the ACD solution on the reactivity against the hemagglutination, and the erythrocytes treated with 16 percent of formalin showed the reactivity of higher level than that of the above two kinds of erythrocytes. 12. There is no difference between the saline and the isotonic buffer solution on the reaction of hemagglutination.

• Applied Microscopy
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• 제18권2호
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• pp.167-176
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• 1988

Extrachromosomal circular DNA complexes from erythrocytes and splenocytes isolated from Carassius carassius were examined by mica-press-absorption method. The method was described that released small polydisperse circular DNA molecules in situ from the erythrocytes and the splenocytes and that allows selective observation of the small circular DNA complexes bound to cellular components. The released polydisperse circular DNA complexes were absorbed preferentially on mica in a divalent cation-free medium then processed for electron microscopy. Small circular DNAs showed a heterogeneous size distribution of $2{\sim}10{\mu}m$ with a mean contour length of $4.3{\mu}m$ for the circulating erythrocytes and that of $0.7{\sim}3.6{\mu}m$ with a mean contour of length $2.04{\mu}m$ for the splencytes. Cells contained $100{\sim}300$ copies and $300{\sim}700$ copies obtained from the erythrocytes and the splenocytes, repectively. Possible biological functional implications for size distribution of extrachromosomal circular DNAs are discussed.

• 대한수의학회지
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• 제32권3호
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• pp.403-406
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• 1992

The study was attempted to scrutinize the normal osmotic fragility of erythrocytes in domestic poultry such as chicken, quail and duck, making a comparison with that in domestic mammalia such as dog and pig. Osmotic fragility of erythrocytes was determined on blood samples from 10 healthy adult animals in each species. Optical initial hemolysis of erythrocytes occurred at $0.395{\pm}0.03%$ NaCl for chicken, $0.410{\pm}0.03%$ for duck, $0.440{\pm}0.02%$ for quail, $0.470{\pm}0.05%$ for dog and $0.560{\pm}0.03%$ for pig. Optical complete hemolysis of erythrocytes occurred at $0.270{\pm}0.02%$ NaCl for chicken, $0.305{\pm}0.03%$ for duck, $0.360{\pm}0.02%$ for quail, $0.370{\pm}0.03%$ for dog and $0.455{\pm}0.03%$ for pig. In other words, erythrocytes of poultry have stronger resistance to osmotic lysis than those of mammalia, showing the strongest resistance in chicken among the tested poultry.

• Toxicological Research
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• 제22권4호
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• pp.397-402
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• 2006

Lipid mediator such as lysophosphatidic acid (LPA) plays an important role in inflammation and wound heating, has been recently reported to induce influx of extracellular calcium into erythrocytes. This elevation in intracellular calcium level may cause destruction of membrane asymmetry and procoagulant microvesicle formation. Thus, we investigated if the lipid mediator could induce microvesicle formation as a result of extracellular calcium influx in human erythrocytes. Treatment with lipid mediator to erythrocytes resulted in microvesicle generation In a concentration-, time-dependent manner. Microvesicles formed expressed procoagulant phosphatidylserine (PS) on their surface membrane significantly as well. LPA did not affect the band 3 phosphorylation which is involved in morphological change in erythrocytes. Pretreatment with suramin did not inhibit LPA-induced microvesicle generation, suggesting microvesicle generation was not receptor-dependent pathway. Depletion of intracellular ATP levels in erythrocytes was suggested to be one of the mechanism for these events.

• 대한미생물학회지
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• 제22권3호
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• pp.225-232
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• 1987

Authors studied on the isolation of V. damsela from sea water, fish and shellfish at the Keoje Hae keumkang on the southern sea and at Hongdo island and Heucksan island on the western sea of Korea from May to September in 1986. Authors investigated for the isolated strains to bacteriological identification, hemolysis about various erythrocytes and antibiotic susceptibilities. The results obtained were as follows: 1. V. damsela was isolated 14 strains from total 383 specimens; 233 cases of sea water, 40 cases of fish and 110 cases of shellfish, respectively. Eight strains were isolated from sea water and 6 strains were isolated from shellfish. 2. The biochemical characteristics which differentiate it from other Vibrio species were indole negative, ornithine negative, Voges-Proskauer positive, arginine positive, galactose positive, glucose positive, maltose positive, mannose positive, trehalose positive, and growth in nutrient broth with 1% to 6% NaCl. 3. On hemolysis reaction on blood agar media using human, rabbit and guinea pig erythrocytes, human erythrocytes were 11 strain positive, rabbit erythrocytes were 12 strain positive and guinea pig erythrocytes were 13 strain positive. 4. Senistivity test using with chemotherapeutic agents of "BioLab" Microbial Sensitivity Test Discs were generally sensitived to amikacin, ampicillin, cephalothin, chloramphenicol, clindamycin, erythromycin, gentamycin, kanamycin, methicillin, penicillin, streptomycin, tetracycline and tobramycin, respectively, but were resistant to lincomycin.

• 대한수의사회지
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• 제7권1호
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• pp.1-3
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• 1963

Fazekas observed that fowl or human erythrocytes treated with a modifying dose of potassium pe-riodate remained agglutinable by influenza virus tut the adsorbed virus did not elute spontaneously. In this study, chicken erythrocytes treated with the high d

• Journal of Pharmaceutical Investigation
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• 제4권1_2호
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• pp.25-30
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• 1974

The adsorption of nalidixic acid on human erythrocytes was found to expressed by Freundlich's isotherm. The amount of adsorption of nalidixic acid on erythrocytes increased with an increase of pH. The adsorption of nalidixic acid on human plasma was found to expressed at Scatchard's equation by the equilibrium dialysis method. An influence of pH on adsorption of nalidixic acid to human plasma proteins was studies at pH 4-10. It was found that the degree of adsorption increase with the increase of pH from 4-6, but descreased above pH 9.

• 한국작물학회지
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• 제25권1호
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• pp.72-76
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• 1980

The present studies were carried out to develope a simple quantitative analysis for saponins in ginseng by hemolysis. Erythrocytes from pig, rabbit, human and cattle were useful for this purpose. Erythrocytes could be stored at $4^{\circ}C$for 9 days without altering the sensitivity to saponins. Ginsenoside-Rb_1 did hemolysis, but Ginsenoside-Re protected erythrocytes from hemolysis. The following formula is proposed for calculation of saponins from ginseng extract: X=120$\mu$g $\times$$V_2/V_1$