Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Microvesicle Generation by Lipid Mediator in Erythrocytes

Lipid Mediator에 의한 적혈구 Microvesicle 생성에 대한 연구

  • Chung, Seung-Min (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Bae, Ok-Nam (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Noh, Ji-Yoon (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kim, Su-Jin (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Lim, Kyung-Min (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Chung, Jin-Ho (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
  • 정승민 (서울대학교 약학대학 종합약학연구소) ;
  • 배옥남 (서울대학교 약학대학 종합약학연구소) ;
  • 노지윤 (서울대학교 약학대학 종합약학연구소) ;
  • 김수진 (서울대학교 약학대학 종합약학연구소) ;
  • 임경민 (서울대학교 약학대학 종합약학연구소) ;
  • 정진호 (서울대학교 약학대학 종합약학연구소)
  • Published : 2006.12.30
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Abstract

Lipid mediator such as lysophosphatidic acid (LPA) plays an important role in inflammation and wound heating, has been recently reported to induce influx of extracellular calcium into erythrocytes. This elevation in intracellular calcium level may cause destruction of membrane asymmetry and procoagulant microvesicle formation. Thus, we investigated if the lipid mediator could induce microvesicle formation as a result of extracellular calcium influx in human erythrocytes. Treatment with lipid mediator to erythrocytes resulted in microvesicle generation In a concentration-, time-dependent manner. Microvesicles formed expressed procoagulant phosphatidylserine (PS) on their surface membrane significantly as well. LPA did not affect the band 3 phosphorylation which is involved in morphological change in erythrocytes. Pretreatment with suramin did not inhibit LPA-induced microvesicle generation, suggesting microvesicle generation was not receptor-dependent pathway. Depletion of intracellular ATP levels in erythrocytes was suggested to be one of the mechanism for these events.

Keywords

References

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