• Bulletin of the Korean Chemical Society
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• 제35권5호
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• pp.1279-1284
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• 2014

The binding of TATA-binding protein (TBP) to the TATA-box containing promoter region is aided by many other transcriptional factors including TFIIA and TFIIB. The mechanistic insight into the assembly of RNA polymerase II preinitation complex (PIC) has been gained by either directly altering a function of target protein or perturbing molecular interactions using drugs, RNAi, or aptamers. Aptamers have been found particularly useful for studying a role of a subset of PIC on transcription for their ability to inhibit specific molecular interactions. One major hurdle to the wide use of aptamers as specific inhibitors arises from the difficulty with traditional assays to validate and determine specificity, affinity, and binding epitopes for aptamers against targets. Here, using a technique called the bio-layer interferometry (BLI) designed for a label-free, real-time, and multiplexed detection of molecular interactions, we studied the assembly of a subset of PIC, TBP binding to TATA DNA, and two distinct classes of aptamers against TPB in regard to their ability to inhibit TBP binding to TFIIA or TATA DNA. Using BLI, we measured not only equilibrium binding constants ($K_D$), which were overall in close agreement with those obtained by electrophoretic mobility shift assay, but also kinetic constants of binding ($k_{on}$ and $k_{off}$), differentiating aptamers of comparable KDs by their difference in binding kinetics. The assay developed in this study can readily be adopted for high throughput validation of candidate aptamers for specificity, affinity, and epitopes, providing both equilibrium and kinetic information for aptamer interaction with targets.

• 공업화학
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• 제26권5호
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• pp.581-586
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• 2015

입상 활성탄을 사용하여 수용액으로부터 Safranin의 흡착에 대해 조사하였다. 회분식 실험은 흡착제의 양, 초기농도와 접촉시간과 온도를 흡착변수로 사용하여 수행하였다. 흡착평형자료는 Langmuir, Freundlich 및 Dubinin-Radushkevich식을 사용하여 해석한 결과 Freundlich식이 가장 좋은 일치도를 나타냈다. 평가된 Langmuir 분리계수, $R_L=0.183{\sim}0.254$와 Freundlich 분리계수, 1/n = 0.518~0.547로부터 이 흡착공정이 적절한 처리방법이 될 수 있음을 알았다. 흡착속도실험자료를 유사1차 및 유사2차 반응속도식에 적용해 본 결과는 유사2차반응속도식에 잘 맞는 것으로 나타났다. 음수값의 Gibbs 자유에너지(${\Delta}G=-3.688{\sim}-7.220kJ/mol$)와 양수값의 엔탈피(${\Delta}H=33.126kJ/mol$)는 흡착이 자발적이고 흡열공정으로 진행된다는 것을 나타냈다.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.149-149
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• 2003

To understand environmental paths of the transport and accumulation of endocrine disrupting chemicals (EDCs), a single cell multimedia fate model has been constructed and evaluated. The EDCs of concern were PAHs, Organochlorine Pesticides (OCPs), PCBs, Alkyl phenols, and phthalates. An evaluation model was designed for the multimedia distribution, including air, water, soil, sediment and vegetation. This model was verified using reported values and via monitoring data. Based on collected data, the distribution trends of EDCs with respect to environmental media were analyzed. Those results have applied to the model for the prediction of the spatial and temporal distribution of EDCs in Seoul. Especially, phenol compound, phthalates, PAHs, PCBs and organochlorine pesticides were estimated and the model was verified. This model was successfully conducted to environmental media, such as air (vapor and suspended particles), soils (forest soil, bare soil, and cement-concrete covered soil), water (dissolved and suspended solids), sediment, trees (deciduous and coniferous). The discrepancies between the model prediction and the measured data are approximately within or near a factor of 10 for the PAHs of three rings through that of six rings, implying that multimedia distribution of the PAHs could be predicted with a factor of 10. Concerning about the air equilibrium may be assumed, a fugacity at steady state is similar in all environmental media. Considering the uncertainties of this model, the use of equilibrium models may be sufficient for assessing chemical fates. In this study, a suggestion was made that modeling and estimation of chemicals in environmental multimedia be rigorously evaluated using the measured flux data. In addition, these data should be obtained, for example, from the precise and standardized inventory of the target chemicals. The model (EDC Seoul) will be refined in an on-going research effort and will be used to support decision-making concerning the management of EDCs.

• 국제지역연구
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• 제22권3호
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• pp.201-219
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• 2018

본고는 산출물 시장에 있어서 독점적으로 행동하는 기업을 고려하여 담보(유담보 융자, 무담보융자)에 의한 차입계약이 차주인 기업의 비용최소화를 통해 비용함수의 형태를 결정하는 모델을 구축하였다. 또한 기업이 금융시장에서의 차입계약이 산출물시장에서 시장균형과 경제후생에 미치는 영향에 대한 분석을 통하여 자기자본이 열악한 기업이 금융기관으로부터 유담보융자에 의해 차입을 실시하면 담보의 범위 내에서 신용을 받을 수 밖에 없는 차입제약에 직면하게 된다는 사실을 증명하였다. 따라서 기업이 생산요소인 자본재를 담보로 설정할 때 생산 요소의 투입비율에 왜곡현상이 발생하며 기업이 금리가 높은 무담보 융자에 의해 대출행위가 이루어지면 한계비용은 상승하기 때문에 기업은 자기이윤을 최대화하는 차입계약을 선택하게 된다. 그러나 기업이 차입계약을 선택할 경우 소비자와 경제전체에 바람직한 현상은 아니며 전체적으로 경제후생을 악화시킨다는 것이 본고의 이론분석을 통한 결과로 볼 수 있다.

• Structural Engineering and Mechanics
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• 제48권6호
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• pp.849-878
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• 2013

This paper consists of two parts, which broadly examines solution techniques abilities for the structures with geometrical nonlinear behavior. In part I of the article, formulations of several well-known approaches will be presented. These solution strategies include different groups, such as: residual load minimization, normal plane, updated normal plane, cylindrical arc length, work control, residual displacement minimization, generalized displacement control, modified normal flow, and three-parameter ellipsoidal, hyperbolic, and polynomial schemes. For better understanding and easier application of the solution techniques, a consistent mathematical notation is employed in all formulations for correction and predictor steps. Moreover, other features of these approaches and their algorithms will be investigated. Common methods of determining the amount and sign of load factor increment in the predictor step and choosing the correct root in predictor and corrector step will be reviewed. The way that these features are determined is very important for tracing of the structural equilibrium path. In the second part of article, robustness and efficiency of the solution schemes will be comprehensively evaluated by performing numerical analyses.

• 제3의학
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• 제1권2호
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• pp.39-55
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• 1996

The theory of seven emotions is a unique theory in oriental medicine which describes the mutual relationship between body and mind of human. Although, the term 'Seven emotions' was not clearly indicated in ${\ulcorner}$The Yellow Emperor's Internal Classic(黃帝內經)${\lrcorner}$, it is appeared in ${\ulcorner}$A Treatise on the Three Catagories of Cause of Diseases(三因方)${\lrcorner}$ written by Chen Yan(陳言) in South-Song Dynasty. It seemed that Chen Yan explained seven emotions as the internal etiologic factor according to the classification of seven emotions of ${\ulcorner}$Ye-Gi(禮記)${\lrcorner}$ under the academic influence during Song Dynasy which emphasized more on the standard of right and wrong rather than individual emotion. Meditation or consideration modulates the function of spleen and stomach and the metabolism of blood and body fluid and it also controls the various emotions and maintains the equilibrium of human body. Human emotions are influenced by the changes of nature and deeply related to time and space including social-environmental factors. The function and strength of seven emotions: joy, anger, anxiety, worry, grief, apprehension and fright are determined by the external stimulation as the causes of illness.

• 한국CDE학회논문집
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• 제21권1호
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• pp.90-98
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• 2016

When ships and offshore plants are flooded or the floating crane is equipped with a heavy object, these floating structures are excessively inclined. In this case, immersion, heel, and trim affecting the hydrostatic restoration performance are very large and are coupled each other. In this paper, in order to calculate a static equilibrium position of floating structures with excessive inclination, the nonlinear governing equations were constructed by sequential linearization. In the governing equation, the immersion, heel, and trim are fully coupled, and the equations are represented using a plane area, a primary moment, and a moment of inertia of the water plane area. Therefore, it is possible to calculate the additional factor related the water plane area for estimating stability. Position and orientation of the floating structure are obtained by iterative calculation. The calculated results are compared with the previous studies in the aspect to the performance and the accuracy.

• 자원환경지질
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• 제33권1호
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• pp.77-88
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• 2000

The goal of this study is to assess the spatial distribution of natural slopes and cutting slopes under would-be development. For this goal, a quantitative slope stability analysis method using GIS integrated with a computer program was developed. Through field investigations, the discontinuity parameters were collected such as orientation of discontinuity, persistence, spacing, JRC, JCS, and water depth. The distributions were interpolated from the ordinary kriging method in ARC/INFO GIS after variogram analysis. The layers showing all parameters needed for limit equilibrium analysis were constructed. The final layer using GIS works composed of 162,352 polygons, that is, unit slopes. The rock slope stability analysis program was coded by C++ language. This program can calculate geometrical vectors related to rock block failures using input orientation data and direction and dimension of strength to occur failure. Also, this can calculate shear strength of joints through empirical equations and quantitative factors of safety. This methodology was applied to the study area which is located in Jaecheon city and Danyang-gun of the northeastern Keumsu is about 135$km^2$. As a result, the study area was entirely stable but unstable, that is, factor of safety less than 1.0dominantly at the slopes near Keumsil, Daejangri, Keumsungmyun and Sojugol, Mt. Dongsan, Juksongmyun by the natural slope stability analysis. Assuming the cutting slope showing the same direction immediate, and quantitative analysis of factors of safety for a regional area could be conducted through GIS integrated with a computer program of limit equilibrium.

• 한국해양공학회지
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• 제28권6호
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• pp.527-532
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• 2014

The thickness of Arctic sea ice is a particularly significant factor in Arctic shipping and other ice-related research areas such as scientific sea ice investigations and Arctic engineering. In this study, the relation between the measured sea ice thickness and freeboard on the Chukchi and Beaufort Seas during the 2010 and 2011 Arctic cruise of the icebreaking research vessel "Araon" were considered. An assumption of hydrostatic equilibrium was used to estimate the ice thickness as a function of the freeboard. Then, to examine the degree of error, a sensitivity analysis of the thickness estimation of the sea ice was conducted. The error in the density and depth of the snow and the error in the density of the seawater were subordinate parameters, but the density of the ice and the freeboard were the primary parameters in the error calculation. The presented relation formula showed fairly close agreement between the calculated and measured results at a freeboard of >0.24 m.

• 전기학회논문지P
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• 제66권3호
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• pp.129-134
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• 2017

We investigated theoretically the quantum optical transition properties of Si, in quasi 2-Dimensinal Landau splitting system, based on quantum transport theory. We apply the quantum transport theory (QTR) to the system in the confinement of electrons by square well confinement potential under linearly polarized oscillating field. We use the projected Liouville equation method with Equilibrium Average Projection Scheme (EAPS). In order to analyze the quantum transition, we compare the temperature and the magnetic field dependencies of the QTLW and the QTLS on four transition processes, namely, the intra-leval transition process, the inter-leval transition process, the phonon emission transition process and the phonon absorption transition process.