• Bulletin of the Korean Chemical Society
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• v.35 no.5
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• pp.1279-1284
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• 2014

The binding of TATA-binding protein (TBP) to the TATA-box containing promoter region is aided by many other transcriptional factors including TFIIA and TFIIB. The mechanistic insight into the assembly of RNA polymerase II preinitation complex (PIC) has been gained by either directly altering a function of target protein or perturbing molecular interactions using drugs, RNAi, or aptamers. Aptamers have been found particularly useful for studying a role of a subset of PIC on transcription for their ability to inhibit specific molecular interactions. One major hurdle to the wide use of aptamers as specific inhibitors arises from the difficulty with traditional assays to validate and determine specificity, affinity, and binding epitopes for aptamers against targets. Here, using a technique called the bio-layer interferometry (BLI) designed for a label-free, real-time, and multiplexed detection of molecular interactions, we studied the assembly of a subset of PIC, TBP binding to TATA DNA, and two distinct classes of aptamers against TPB in regard to their ability to inhibit TBP binding to TFIIA or TATA DNA. Using BLI, we measured not only equilibrium binding constants ($K_D$), which were overall in close agreement with those obtained by electrophoretic mobility shift assay, but also kinetic constants of binding ($k_{on}$ and $k_{off}$), differentiating aptamers of comparable KDs by their difference in binding kinetics. The assay developed in this study can readily be adopted for high throughput validation of candidate aptamers for specificity, affinity, and epitopes, providing both equilibrium and kinetic information for aptamer interaction with targets.

• Applied Chemistry for Engineering
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• v.26 no.5
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• pp.581-586
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• 2015

Adsorption of Safranin using granular activated carbon from aqueous solution was investigated. Batch experiments were carried out as a function of adsorbent dose, initial concentration, contact time and temperature. The equilibrium adsorption data were fitted to Langmuir, Freundlich and Dubinin-Radushkevich isotherm models. Based on an estimated Langmuir separation factor, $R_L=0.183{\sim}0.254$ and a Freundlich separation factor, 1/n = 0.518~0.547, this process could be employed as an effective treatment method. Adsorption data were also modeled using the pseudo-first and second-order kinetic equations. It was shown that the pseudo-second-order kinetic equation could best describe the adsorption kinetics. The negative Gibbs free energy (${\Delta}G=-3.688{\sim}-7.220kJ/mol$) and positive enthalpy (${\Delta}H=33.126kJ/mol$) indicated that the adsorption process was spontaneous and endothermic.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.05a
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• pp.149-149
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• 2003

To understand environmental paths of the transport and accumulation of endocrine disrupting chemicals (EDCs), a single cell multimedia fate model has been constructed and evaluated. The EDCs of concern were PAHs, Organochlorine Pesticides (OCPs), PCBs, Alkyl phenols, and phthalates. An evaluation model was designed for the multimedia distribution, including air, water, soil, sediment and vegetation. This model was verified using reported values and via monitoring data. Based on collected data, the distribution trends of EDCs with respect to environmental media were analyzed. Those results have applied to the model for the prediction of the spatial and temporal distribution of EDCs in Seoul. Especially, phenol compound, phthalates, PAHs, PCBs and organochlorine pesticides were estimated and the model was verified. This model was successfully conducted to environmental media, such as air (vapor and suspended particles), soils (forest soil, bare soil, and cement-concrete covered soil), water (dissolved and suspended solids), sediment, trees (deciduous and coniferous). The discrepancies between the model prediction and the measured data are approximately within or near a factor of 10 for the PAHs of three rings through that of six rings, implying that multimedia distribution of the PAHs could be predicted with a factor of 10. Concerning about the air equilibrium may be assumed, a fugacity at steady state is similar in all environmental media. Considering the uncertainties of this model, the use of equilibrium models may be sufficient for assessing chemical fates. In this study, a suggestion was made that modeling and estimation of chemicals in environmental multimedia be rigorously evaluated using the measured flux data. In addition, these data should be obtained, for example, from the precise and standardized inventory of the target chemicals. The model (EDC Seoul) will be refined in an on-going research effort and will be used to support decision-making concerning the management of EDCs.

• International Area Studies Review
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• v.22 no.3
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• pp.201-219
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• 2018

This paper suggested a theoretical model, in which a security-based(secured loan, non-secured loan) credit agreement determines the form of corporate cost function through a loaning company's cost minimization in the light of a company which behaves monopolistically in product markets. Also, this paper analyzed the influence of a corporate credit agreement on market equilibrium, and economic welfare in product markets. As a result, it was found that in case a company, whose equity capital is small, implements borrowing based on a secured loan from a financial institution, the company comes to face borrowing restraints, in which the company has no choice but to get a loan within the scope of securities. When a company offers its capital goods, i.e. a production factor, as a security, there occurs a distortion to the production factor input ratio. Meanwhile, when a company comes to get a loan based on an unsecured loan, for which the interest rate is high, marginal cost rises; accordingly, the company comes to choose a credit agreement aiming at maximizing its profits. However, a company's choice of a credit agreement is not quite desirable from a consumer's viewpoint, and from the whole economic point of view; overall, such a choice is likely to aggravate economic welfare.

• Structural Engineering and Mechanics
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• v.48 no.6
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• pp.849-878
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• 2013

This paper consists of two parts, which broadly examines solution techniques abilities for the structures with geometrical nonlinear behavior. In part I of the article, formulations of several well-known approaches will be presented. These solution strategies include different groups, such as: residual load minimization, normal plane, updated normal plane, cylindrical arc length, work control, residual displacement minimization, generalized displacement control, modified normal flow, and three-parameter ellipsoidal, hyperbolic, and polynomial schemes. For better understanding and easier application of the solution techniques, a consistent mathematical notation is employed in all formulations for correction and predictor steps. Moreover, other features of these approaches and their algorithms will be investigated. Common methods of determining the amount and sign of load factor increment in the predictor step and choosing the correct root in predictor and corrector step will be reviewed. The way that these features are determined is very important for tracing of the structural equilibrium path. In the second part of article, robustness and efficiency of the solution schemes will be comprehensively evaluated by performing numerical analyses.

• Journal of The Association for Neo Medicine
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• v.1 no.2
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• pp.39-55
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• 1996

The theory of seven emotions is a unique theory in oriental medicine which describes the mutual relationship between body and mind of human. Although, the term 'Seven emotions' was not clearly indicated in ${\ulcorner}$The Yellow Emperor's Internal Classic(黃帝內經)${\lrcorner}$, it is appeared in ${\ulcorner}$A Treatise on the Three Catagories of Cause of Diseases(三因方)${\lrcorner}$ written by Chen Yan(陳言) in South-Song Dynasty. It seemed that Chen Yan explained seven emotions as the internal etiologic factor according to the classification of seven emotions of ${\ulcorner}$Ye-Gi(禮記)${\lrcorner}$ under the academic influence during Song Dynasy which emphasized more on the standard of right and wrong rather than individual emotion. Meditation or consideration modulates the function of spleen and stomach and the metabolism of blood and body fluid and it also controls the various emotions and maintains the equilibrium of human body. Human emotions are influenced by the changes of nature and deeply related to time and space including social-environmental factors. The function and strength of seven emotions: joy, anger, anxiety, worry, grief, apprehension and fright are determined by the external stimulation as the causes of illness.

• Korean Journal of Computational Design and Engineering
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• v.21 no.1
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• pp.90-98
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• 2016

When ships and offshore plants are flooded or the floating crane is equipped with a heavy object, these floating structures are excessively inclined. In this case, immersion, heel, and trim affecting the hydrostatic restoration performance are very large and are coupled each other. In this paper, in order to calculate a static equilibrium position of floating structures with excessive inclination, the nonlinear governing equations were constructed by sequential linearization. In the governing equation, the immersion, heel, and trim are fully coupled, and the equations are represented using a plane area, a primary moment, and a moment of inertia of the water plane area. Therefore, it is possible to calculate the additional factor related the water plane area for estimating stability. Position and orientation of the floating structure are obtained by iterative calculation. The calculated results are compared with the previous studies in the aspect to the performance and the accuracy.

• Economic and Environmental Geology
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• v.33 no.1
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• pp.77-88
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• 2000

The goal of this study is to assess the spatial distribution of natural slopes and cutting slopes under would-be development. For this goal, a quantitative slope stability analysis method using GIS integrated with a computer program was developed. Through field investigations, the discontinuity parameters were collected such as orientation of discontinuity, persistence, spacing, JRC, JCS, and water depth. The distributions were interpolated from the ordinary kriging method in ARC/INFO GIS after variogram analysis. The layers showing all parameters needed for limit equilibrium analysis were constructed. The final layer using GIS works composed of 162,352 polygons, that is, unit slopes. The rock slope stability analysis program was coded by C++ language. This program can calculate geometrical vectors related to rock block failures using input orientation data and direction and dimension of strength to occur failure. Also, this can calculate shear strength of joints through empirical equations and quantitative factors of safety. This methodology was applied to the study area which is located in Jaecheon city and Danyang-gun of the northeastern Keumsu is about 135$km^2$. As a result, the study area was entirely stable but unstable, that is, factor of safety less than 1.0dominantly at the slopes near Keumsil, Daejangri, Keumsungmyun and Sojugol, Mt. Dongsan, Juksongmyun by the natural slope stability analysis. Assuming the cutting slope showing the same direction immediate, and quantitative analysis of factors of safety for a regional area could be conducted through GIS integrated with a computer program of limit equilibrium.

• Journal of Ocean Engineering and Technology
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• v.28 no.6
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• pp.527-532
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• 2014

The thickness of Arctic sea ice is a particularly significant factor in Arctic shipping and other ice-related research areas such as scientific sea ice investigations and Arctic engineering. In this study, the relation between the measured sea ice thickness and freeboard on the Chukchi and Beaufort Seas during the 2010 and 2011 Arctic cruise of the icebreaking research vessel "Araon" were considered. An assumption of hydrostatic equilibrium was used to estimate the ice thickness as a function of the freeboard. Then, to examine the degree of error, a sensitivity analysis of the thickness estimation of the sea ice was conducted. The error in the density and depth of the snow and the error in the density of the seawater were subordinate parameters, but the density of the ice and the freeboard were the primary parameters in the error calculation. The presented relation formula showed fairly close agreement between the calculated and measured results at a freeboard of >0.24 m.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.66 no.3
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• pp.129-134
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• 2017

We investigated theoretically the quantum optical transition properties of Si, in quasi 2-Dimensinal Landau splitting system, based on quantum transport theory. We apply the quantum transport theory (QTR) to the system in the confinement of electrons by square well confinement potential under linearly polarized oscillating field. We use the projected Liouville equation method with Equilibrium Average Projection Scheme (EAPS). In order to analyze the quantum transition, we compare the temperature and the magnetic field dependencies of the QTLW and the QTLS on four transition processes, namely, the intra-leval transition process, the inter-leval transition process, the phonon emission transition process and the phonon absorption transition process.