• Applied Microscopy
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• 제24권1호
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• pp.41-58
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• 1994

The present studies were designed to determine the feasibility of using the rat tracheal epithelium as models for induction of aging. The ultrastructural and cytochemical changes of tracheal epithelium were investigated in rats at ages of five, twelve and twenty four months. Some major changes in the tracheal epithelium with advancing age were observed by electron microscopy. The results were summarized as fellow: 1. With the advance of age, lysosome, vacuole and multivesicular bodies were increased in number and numerous myelinoid bodies were observed in cytoplasm of ciliated cells. 2. In goblet cell, serous cell and brush cell lysosome and myelinoid bodies were increased in number with the advance of age, and an myelinoid bodies was often found within the secretory granule. 3. Cytochemical studies showed that acid phosphatase activities was observed in multivesicular bodies and lysosome, strong activities with the advance of age. And alkaline phosphatase activity are observed in microvilli, granule and lateral membrane of secretory granule cells, and strong activities with age. Consequently suggest that with the advance of age, tracheal epithelium show ultrastructural and cytochemical alteration of some kind of cell organelles in all kind of cell.

• 대한수의학회지
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• 제29권3호
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• pp.231-237
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• 1989

Morphological features of the vomeronasal organ of both Korean native cattle and Korean native goat were studied by gross, microscopic and histochemical examinations. Anatomical characteristics of the vomeronasl organ were similar in both Korean native cattle and Korean native goats. The vomeronasal organ is a tubular structure situated bilaterally at the base of the nasal septum, and enclosed by hyaline cartilage. Its lumen is semilunar to crescent in transverse sections. It join with the incisive duct through narrow duct. The lumen of the vomeronasal organ is lined with sensory and respiratory epithelia. The distribution pattern of vomeronasal mucosal epithelia varied by the position. In the anterior portion joining with nasal cavity, the lumen is lined with only respiratory epitheliu. In the middle portion, sensory epithelium appeared on the medial side, and respiratory epithelium on the lateral side. In the posterior, it is lined with sensory epithelium on the ventral side and lined with respiratory epithelium on the dorsal side. The vomeronasal gland composed of mucous and serous acini are distributed in the lamina propria under the respiratory epithelium, where venous sinuses are also well developed.

• 한국가금학회지
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• 제26권3호
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• pp.171-177
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• 1999

This study was conducted for the histological observation of the infundibulum of the oviduct of the laying Korean native pheasants. The results are as follows : 1. The infundibular wall is composed of the epithelium, lamina propria, muscle layer(inner circular and outer longitudinal muscle), and serosa. The funnel lip is divided into the inner, and outer lip of the epithelium and muscle layer. 2. The epithelium of the funnel lip and most region of the cranial part of the funnel are composed of ciliated columnar cells. In the surface and lateral part of the folds, ciliated cells and non-ciliated secretory cells tend to alternate in the epithelium of the caudal funnel and the necks, but are also found in groups of the simple cuboidal epithelium at the bases of the grooves between the ridges and tubular glands found in the subepithelium. 3. The secretory material of the non-ciliated secretory cills contains PAS-positive and alcian blue-positive granules, and these materials show purple colour in the basic fuchsin-methylene blue stain. 4. The cells of the glandular groove and tubular gland of the neck portion of the oviduct mostly show weak PAS-positive, and alcian blue stain negative reaction. The tubular gland cells of the infndibulum contain pink of purple colour granules, and without reaction in the anterior neck portion of the infundibulum in basic fuchsin and methylene blue stain.

• 한국어병학회지
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• 제18권2호
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• pp.119-124
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• 2005

Two species of myxosporean parasites - Parvicapsula anisocaudata and an unidentified myxosporean were found in the lumina of renal tubules and the tubular epithelium, respectively, from cultured olive flounder, Paralichthys olivaceus in Korea. The latter was also seen in interstitial tissue of spleen and interrenal gland of the head kidney. Group of pseudoplasmodia of P. anisocaudata were firmly attached on the epithelium of renal tubules through pseudopodia. In the renal tubule epithelium, a group of unidentified myxosporean trophozoites, which were 2-3 times larger than intraluminal trophozoites of P. anisocaudata, was observed. The parasites being burst out into the lumen was occasionally encountered with partial break of the epithelium. Although infection of P. anisocaudata and unidentified myxosporean parasites did not induce any cellular reaction of the host, occlusion of renal tubules and rupture of renal epithelium would impact negatively on the renal functions of severely infected fish.

• Toxicological Research
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• 제33권3호
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• pp.191-203
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• 2017

Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 404 and OECD TG 405 but it has been criticized with respect to animal welfare due to invasive and cruel procedure. To replace it, diverse alternatives have been developed: (i) For Draize eye irritation test, organotypic assay, in vitro cytotoxicity-based method, in chemico tests, in silico prediction model, and 3D reconstructed human cornealike epithelium (RhCE); (ii) For Draize skin irritation test, in vitro cytotoxicity-based cell model, and 3D reconstructed human epidermis models (RhE). Of these, RhCE and RhE models are getting spotlight as a promising alternative with a wide applicability domain covering cosmetics and personal care products. In this review, we overviewed the current alternatives to Draize test with a focus on 3D human epithelium models to provide an insight into advancing and widening their utility.

• Applied Microscopy
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• 제27권1호
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• pp.1-12
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• 1997

Dorsal and ventral epithelium of leech, Whitemenia edentula, were observed, using both light and electron-microscope. Results are as follows. Epithelia are composed of simple columnar or irregularly-shaped epithelium, the transveral folds were seen in the cross-sectioned dorsal and ventral epithelia, but only longitudinal folds(depth $500{\mu}m$, width $350{\mu}m$) were discovered in the ventral epithelium. As to muscular tissue under the dorsal epithelium of Whitemenia edentula, that of longitudinal muscle layer is thicker and more developed than that of circular muscle layer. Seven gland cells (type-A, B, C, D, E, F and H cells) and one pigment cell (type-G cell) were discovered in the whitemenia edentula. Those were identified as three kinds of the neutral mucopolysaccharide granoles (type-A, B and F cells), two kinds of acidic mucopolysaccharide granules (type-C and D cells), and two kinds of non-reactive granules respectively (type-E and F cells). The glanules of the type-A and type-B gland cells ae electronly high-dense, and surrounded by the muscular tissue. As to the distribution of gland cells, the type-A, type-B, type-C, type-D, type-I and type-H gland cells were discovered only between the connective tissue and the circular muscle layer, while type-F gland cells were discovered only between tile circular muscle layer and the longitudinal muscle layer.

• 대한치과의사협회지
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• 제55권7호
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• pp.489-496
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• 2017

Oral and maxillofacial cyst is defined as an pathogenic cavity with an lining epithelium and connective tissue wall. Cysts of the jaws and periapical regions vary in histogenesis, treatment and prognosis. Cysts with similar clinical and radiographic can be shown different histopathologic features. Cysts are classified into odontogenic cysts and nonodontogenic cysts. Cysts are also divied into true cysts and pseudocyst. True cysts are lined with an epithelium, however pseudocysts are not lined with epithelium. A periapical cyst, dentigerous cyst and odontogenic keratocyst is clinically common and important lesions at dental clinic.

• 대한의생명과학회지
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• 제10권3호
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• pp.275-283
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• 2004

The annual changes in testis weight and diameter of seminiferous tubules, and the seminiferous epithelium cycle of Tamias sibiricus were studied by light microscope. Testis weight and diameter of seminiferous tubule are significantly increased from January to July, and decreased rapidly to the size from August to December. Spermatogenesis occurs from January to July, and spermatocytogenesis are produced from August to December. The cycle of the seminiferous epithelium was divided into 12 stages during the development of spermatids as a changes of the nucleus and acrosomal structure, presence and/or absence of residual body, appearance and/or absence of sperm tail and meiotic figure and spermiation. The dark type spermatogonia (Ad) are appeared in all stages (I ~ XII), and the spermatids of step 10 are observed at I, II, X and XII stages. The spermatids of step 11 are appeared in III and IV stages, only the step 12 spermatid observed in V stage.

• 대한의생명과학회지
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• 제10권4호
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• pp.435-445
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• 2004

The characteristics of the testis and the annual cycle of the seminiferous epithelium of the Miniopterus schreibersi fuliginosus were examined by optical microscopy. The testis weight and diameter of the seminiferous tubules were increased gradually from May to July, and the highest activity was observed in August. The size then decreased rapidly from October. Spermatogenesis began in May, peaked in August, and was suspended from October to April in the following year. Spermatocytogenesis were produced from May to July. Spermiogenesis occurred from August to September. In particular, immature spematogenic cells in the seminiferous tubules were engulfed by the phagocytosis of Sertoli cells in October. From November to April, the seminiferous tubuly contained only Sertoli cells and Ad spermatogonia. Therefore, the periodic changes in the seminiferous epithelium of M. S. fuliginosus suggest that a long hibernation is an adaptive strategy for the preservation of energy and the regulation of the breeding cycle.

• BMB Reports
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• 제44권7호
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• pp.435-445
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• 2011

Serous ovarian cancer is one of the most lethal gynecological malignancies. Progress on effective diagnostics and therapeutics for this disease are hampered by ambiguity as to the cellular origins of this histotype of ovarian cancer, as well as limited suitable animal models to analyze early stages of disease. In this report, we will review current animal models with respect to the two proposed progenitor cells for serous ovarian cancer, the ovarian surface epithelium and the fallopian tube epithelium.