• 제목/요약/키워드: enzymatic pretreatment

검색결과 181건 처리시간 0.029초

알칼리 전처리가 굴참나무의 효소 당화에 미치는 영향 (Impact of Alkali Pretreatment to Enzymatic Hydrolysis of Cork Oak (Quercus Variabilis))

  • 윤수영;신수정
    • 펄프종이기술
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    • 제46권6호
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    • pp.1-7
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    • 2014
  • Dissolving part of xylan and lignin in lignocellulosic biomass by base can be used as pretreatment technique. Cork oak was pretreated with sodium hydroxide solution and the pretreatment effects were evaluated with two critical factors - NaOH concentration and pretreatment temperature. Some of xylan and lignin were removed by base pretreatment. At $90^{\circ}C$ and 13% NaOH pretreatment, 22.0% of lignin and 78.8% of xylan removed by base treatment. Enzymatic hydrolysis of cork oak which was pretreated at higher temperature or concentration was further improved. After pretreatment of cork oak with 13% NaOH at $90^{\circ}C$, the conversion rate of cellulose to fermentable sugars were reached up to 91.3%. At ethanol fermentation with enzymatic hydrolysate from different pretreatment conditions, all enzymatic saccharification liquids were well fermented by Saccharomyces cerevisiae.

꽃게(Blue Crab) 가공 식품 제조 공정상 발생된 폐수 및 폐기물의 혐기성 생분해 가능성(II) -공정상 발생된 폐기물의 혐기성 생분해 가능성 증대를 위한 효소적 전처리- (Anaerobic Bioconversion Potential of Blue Crab Processing Waste and Wastewater(II) -Enzymatic Pretreatment for Improving the Anaerobic Bioconversion Potential of Blue Crab Processing Wastes-)

  • 이형집
    • 상하수도학회지
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    • 제12권2호
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    • pp.115-126
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    • 1998
  • In use of anaerobic bioconversion shellfish wastes present special problems, since the chitinous structures in the shell faction degrade very slowly in an anaerobic environment. Enzymatic pretreatment method was evaluated for improving the anaerobic bioconversion potential of blue crab processing wastes. An enzymatic pretreatment using chitinase enhanced the ultimate methane yield and biodergradation rate constant for total crab solid wastes by 15% and 19% respectively, above those of the untreated wastes. When the enzymatic pretreatment applied to the shell fraction alone, it resulted in increase of 34% in the ultimate methane yield and 38% in the reaction rate. The results indicate that anaerobic bioconversion of these wastes is technically feasible and enzymatic pretreatment will improve the efficiency of the process.

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Wet Air Oxidation Pretreatment of Mixed Lignocellulosic Biomass to Enhance Enzymatic Convertibility

  • Sharma, A.;Ghosh, A.;Pandey, R.A.;Mudliar, S.N.
    • Korean Chemical Engineering Research
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    • 제53권2호
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    • pp.216-223
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    • 2015
  • The present work explores the potential of wet air oxidation (WAO) for pretreatment of mixed lignocellulosic biomass to enhance enzymatic convertibility. Rice husk and wheat straw mixture (1:1 mass ratio) was used as a model mixed lignocellulosic biomass. Post-WAO treatment, cellulose recovery in the solid fraction was in the range of 86% to 99%, accompanied by a significant increase in enzymatic hydrolysis of cellulose present in the solid fraction. The highest enzymatic conversion efficiency, 63% (by weight), was achieved for the mixed biomass pretreated at $195^{\circ}C$, 5 bar, 10 minutes compared to only 19% in the untreated biomass. The pretreatment under the aforesaid condition also facilitated 52% lignin removal and 67% hemicellulose solubilization. A statistical design of experiments on WAO process conditions was conducted to understand the effect of process parameters on pretreatment, and the predicted responses were found to be in close agreement with the experimental data. Enzymatic hydrolysis experiments with WAO liquid fraction as diluent showed favorable results with sugar enhancement up to $10.4gL^{-1}$.

Optimization of organosolv pretreatment with sulfuric acid for enhancing enzymatic hydrolysis of Pitch Pine (Pinus rigida)

  • 박나현;김혜연;곽기섭;구본욱;어환명;최인규
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2009년도 추계학술대회 논문집
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    • pp.505-505
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    • 2009
  • The object is to optimize the best condition of organosolv pretreatment process with sulfuric acid as a catalyst. As a material, Pitch pine (Pinus rigida) was ground and sieved through 40-mesh screen, and Celluclast and $\beta$-glucosidase were used as enzymes for enzymatic hydrolysis. Pretreatment processes were carried out in the minibomb, and 20 g of materials with 200 ml of 50% ethanol solution (v/v) with 1% sulfuric acid as a catalyst. Pretreatment temperature was varied from $150^{\circ}C$ to $190^{\circ}C$, and time was varied from 0 to 20 min. Then, residual materials were used for enzymatic hydrolysis. The best conditions were selected by estimating followed enzymatic hydrolysis rate and degradable rates after pretreatment process. The highest value of enzymatic hydrolysis rate was obtained as 55 - 60% at 160 and at $180^{\circ}C$, but the value decreased under more severe conditions. As the residual rates decreased under severe conditions, it infered that the decrease of sugar contents limits enzymatic hydrolysis rates. Combined with enzymatic hydrolysis rate, degradable rates and H-factors, the temperatures at $160^{\circ}C$ for 20 min and at $180^{\circ}C$ for 0 min were concluded as the optimized conditions where have the lowest H-factor value for considering energy input.

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Investigation of the Effective Catalyst for Organosolv Pretreatment of Liriodendron tulipifera

  • Koo, Bon-Wook;Gwak, Ki-Seob;Kim, Ho-Yong;Choi, Joon-Weon;Yeo, Hwan-Myeong;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • 제38권2호
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    • pp.149-158
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    • 2010
  • Organosolv pretreatments which utilized sulfuric acid, sodium hydroxide and ammonia as catalysts were conducted to screen the effective catalyst for organosolv pretreatment of Liriodendron tulipifera. The enzymatic hydrolysis was achieved effectively with sulfuric acid (74.2%) and sodium hydroxide (63.7%). They were thus considered as effective catalysts for organosolv pretreatment of L. tulipifera. The organosolv pretreatments with sulfuric acid and sodium hydroxide showed a different behavior on the reaction mechanism. The pretreatment with sulfuric acid increased the biomass roughness and pore numbers. On the other hand, the pretreatment with sodium hydroxide enhanced the surface area due to the size reduction and minor defiberization which were caused by hemicellulose degradation at an initial stage and more defiberization by lignin degradation at a later stage. The organosolv pretreatment with sodium hydroxide was performed at several different conditions to evaluate effectiveness of sodium hydroxide as a catalyst for organosolv pretreatment. According to the results of enzymatic digestibility, the changes of chemical composition and the morphological analysis of pretreated biomass, it was suggested that the pretreatment time impacted primarily on enzymatic hydrolysis. Increase in surface area during the pretreatment was a major cause for improvement in enzymatic digestibility when sodium hydroxide was used as a catalyst.

효소 가수분해 향상을 위해 고압조건에서 Glycerol 수용액을 사용한 폐지의 전처리 (Pretreatment of Wastepaper using Aqueous Glycerol under High Pressure to Enhance Enzymatic Hydrolysis)

  • 서동일;김창준;김성배
    • KSBB Journal
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    • 제29권3호
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    • pp.193-198
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    • 2014
  • Pretreatment of wastepaper using aqueous glycerol under high pressure was studied to enhance the enzymatic hydrolysis. The pretreatment was conducted over a wide range of conditions including temperatures of $150{\sim}170^{\circ}C$, sulfuric acid concentrations of 0.5~1.5%, and reaction times of 30~90 minutes. After the effect of glycerol concentration on the pretreatment performance was investigated, 70% glycerol was selected. As glycerol concentration was increased, higher digestibility was achieved due to higher lignin removal. The optimum condition was found to be around $160^{\circ}C$, 1%, and 60 minutes. At this condition, 60% and 35% of hemicellulose and lignin, respectively, were removed, while only 5% of cellulose was lost. The enzymatic digestibility was 76%, meaning that 73% of the glucan present in the initial substrate was recovered as glucose after enzymatic hydrolysis. Also, it was found that the temperature and acid concentration than the reaction time were more strongly related to the compositional removals and enzymatic digestibility.

전처리 공정에 따른 폐 신문지의 효소 가수분해 특성

  • 문남규;이재환;김성배
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.459-462
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    • 2000
  • The pretreatment of used newspaper for the enzymatic digestion preprocess was performed on a percolation reactor and a batch reactor. The test condition of percolation process was $170^{circ}C$, 60min, 1 mL/min, and 400psi, that of batch was $40^{circ}C$, 3hr. and latm Reaction solutions used in pretreatment process were aqueous ammonia, sulfuric acid, water, and hydrogen-peroxide as an oxidizing agent. As a result, the effect of pretreatment was similar to batch and percolation process, but the yield of enzymatic hydrolysis was higher in batch than percolation. This batch pretreatment enhanced enzymatic hydrolysis rate and increased glucose yield from about 15 to 20%. The inhibition factors influenced the rate of enzymatic hydrolysis was investigated, and the ink contented newspaper was the major factor.

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효소 가수분해 향상을 위해 glycerol 수용액을 사용한 폐지의 전처리 (Pretreatment of Wastepaper using Aqueous Glycerol to enhance Enzymatic Hydrolysis)

  • 서동일;김창준;김성배
    • KSBB Journal
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    • 제28권1호
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    • pp.48-53
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    • 2013
  • Pretreatment of wastepaper using aqueous glycerol was investigated to enhance the enzymatic hydrolysis. The effects of four factors (solid/liquid ratio, glycerol concentration, acid concentration, and reaction time) on the dissolution yield, the removal of cellulose, hemicellulose and lignin, and the enzymatic digestibility were examined at $150^{\circ}C$. The 1/8 of solid/liquid was determined to perform the reaction uniformly, and the 93% of glycerol concentration was found to be a minimum concentration to conduct the reaction under atmospheric pressure. Also, it was found that the acid concentration and reaction time were strongly related to the dissolution yield and the removal of cellulose, hemicellulose and lignin, but moderately to the enzymatic digestibility. At an optimum condition of $150^{\circ}C$, 1 h and 1% acid concentration, 56% and 49% of hemicellulose and lignin, respectively, were removed, while only 4% of cellulose was removed. The enzymatic digestibility at this condition was 86%, meaning that 83% of the glucan present in the initial substrate was converted to glucose. Compared to glycerol with ethylene glycol as a pretreatment solvent, glycerol is much cheaper than ethylene glycol, but ethylene glycol is superior to glycerol in delignification.

Optimization of Bio-based Succinic Acid Production from Hardwood Using the Two Stage pretreatments

  • Jung, Ji Young;Jo, Jong Soo;Kim, Young Wun;Yoon, Byeng Tae;Kim, Choon Gil;Yang, Jae Kyung
    • Journal of the Korean Wood Science and Technology
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    • 제41권2호
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    • pp.111-122
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    • 2013
  • The steam explosion-chemical pretreatment is a more effective wood pretreatment technique than the conventional physical pretreatment by accelerating reactions during the pretreatment process. In this paper, two-stage pretreatment processes of hardwood were investigated for its enzymatic hydrolysis and the succinic acid yield from the pretreated solid. The first stage pretreatment was performed under conditions of low severity to optimize the amount of solid recovery. In the second stage pretreatment washed solid material from the first stage pretreatment step was impregnated again with chemical (alkaline or chlorine-based chemicals) to remove a portion of the lignin, and to make the cellulose more accessible to enzymatic attack. The effects of pretreatment were assessed by enzymatic hydrolysis and fermentation, after the two stage pretreatments. Maximum succinic acid yield (16.1 g $L^{-1}$ and 77.5%) was obtained when the two stage pretreatments were performed at steam explosion -3% KOH.

효소 당화율을 높이기 위해 폐 신문지의 전처리 (Pretreatment of Used Newspaper to Increase Enzymatic Digestibility)

  • 문남규;김성배
    • KSBB Journal
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    • 제16권5호
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    • pp.446-451
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    • 2001
  • 신문지와 같은 폐지지 적합한 전처리 방법을 조사하였다. 조사방법은 전처리 후 기질의 효소 가수분해에 영향을 미칠수 있는 인자, 즉 회분의 양, 기질 크기의 잉크의 유무에 따른 효소 당화율을 측정하였다. 이 세 인자 중 잉크가 효소당화율에 미치는 영향이 가장 커서 잉크를 제거하는 방향으로 전처리 방법을 고안하였다. 먼저 섬유성 기질에서 성능이 입증된 percolation반응기에 의한 전처리 방법을 사용하였다. 그러나 17$0^{\circ}C$에서 전처리된 기질은 잉크가 거의 제거되지 않앗고 효소 당화율도 전처리하지 않은 기질보다 더 낮았다. 그래서 10$0^{\circ}C$이하의 낮은 온도에서 회분식 반응기를 사용하는 방법을 연구했는데 암모니아에 과산화수소를 첨가하여 진탕교반시키는 방법이 가장 효과적이었다. 이 방법의 효소 당화율은 전처리하지 않은 기질보다 약 20% 증가한 85%이어서 신문지와 같은 폐지의 전처리에 아주 효과적이었음을 알수 있었다. 이와 같은 높은 당화율은 암모니아에 첨가한 과산화수소가 잉크제거와 기질을 팽윤시키는 작용을 하기 때문이라고 생각된다.

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