• 대한토목학회논문집
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• 제30권6C호
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• pp.255-263
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• 2010

본 논문은 터널굴착으로 인해 발생된 인접지반에서의 지반변위가 구조물에 미치는 영향을 시공조건(지반손실) 및 지반의 특성을 달리하면서 지반-구조물 상호작용이 고려된 상태에서 조사한 것이다. 터널굴착에 의해 발생된 지반변위에 노출된 4층 블록식구조물이 서로 다른 시공조건(지반손실) 및 지반조건에 노출될 때 발생되는 구조물 거동이 수치해석을 통해 조사되었다. 수치해석을 위한 구조물은 소요전단 및 인장강도 이상의 응력이 발생할 때 구조물에 실제크랙이 발생될 수 있도록 개별요소법(DEM)을 이용하여 모델링되었다. 터널굴착유발 지반변위에 노출된 4층 블록식구조물의 거동 및 손상정도가 지반변위의 크기에 따라 조사되었으며, 발생된 구조물의 거동 및 손상정도는 구조물에 발생한 변형, 크랙크기 및 분포를 고려하여 시공조건(지반손실) 및 지반조건별로 비교되었다. 뿐만아니라, 다양한 시공조건(지반손실) 및 지반조건의 변화에 의해 구조물에 유발될 수 있는 손상정도의 크기가 손상도 예측기준(Son and Cording, 2005)을 사용하여 제시되었다. 이러한 결과들은 향후 터널굴착으로 인해 유발되는 인접구조물의 손상을 제어하고 최소화하는데 필요한 정보를 제공할 것이다.

• Environmental Analysis Health and Toxicology
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• 제29권
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• pp.10.1-10.6
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• 2014

Objectives Cigarette smoking had been recorded as the main cause of impaired endothelium-dependent vasodilation in smokers by reducing nitric oxide (NO), a production of endothelial nitric oxide synthase (eNOS). However, the mechanism of NO impairment via eNOS activity is unclear until now. In this study, cell passage is suggested to be a relevant factor to eNOS expression under cigarette smoking stress. Methods Bovine aortic endothelial cells (BAECs) were chosen as the research subject with passages ranking from 6 to 9 (6P to 9P). After exposure of cigarette smoking extract (CSE) solution, MTT assay and Western blot method were performed to check the cell viability as well as eNOS protein concentration. In these experiments, four concentrations of CSE at 0.5, 1, 2, and 4% were selected for treatment. Results Our results showed that cells almost died at 4% of CSE. Besides, eNOS protein mass had a linear decrease under the increase of CSE concentration. In addition, the effect of CSE on eNOS expression was dissimilar between different passages. Conclusions This study indicated that CSE had effect on both cell viability and eNOS expression. Besides, a reduction in protein mass was matched with the decrease of cell viability due to CSE tress. Last but not least, the response of eNOS protein to different concentration of CSE at different passages was disparate, making the hypothesis about cell passage related inhibition of eNOS caused by CSE solution.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1060-1066
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• 2005

Under environmental stress, such as strong irradiance or nitrogen deficiency, unicellular green algae of the genus Haematococcus accumulate secondary carotenoids, i.e. astaxanthin, in the cytosol. The induction and regulation of astaxanthin biosynthesis in microalgae has recently received considerable attention owing to the increasing use of secondary carotenoids as a source of pigmentation for fish aquacultures, and as a potential drug in cancer prevention as a free-radical quencher. Accordingly, this study generated expressed sequence tags (ESTs) from a library constructed from astaxanthin-induced Haematococcus pluvialis. Partial sequences were obtained from the 5' ends of 1,858 individual cDNAs, and then grouped into 1,025 non-overlapping sequences, among which 708 sequences were singletons, while the remainder fell into 317 clusters. Approximately $63\%$ of the EST sequences showed similarity to previously described sequences in public databases. H. pluvialis was found to consist of a relatively high percentage of genes involved in genetic information processing ($15\%$) and metabolism ($11\%$), whereas a relatively low percentage of sequences was involved in the signal transduction ($3\%$), structure ($2\%$), and environmental information process ($3\%$). In addition, a relatively large fraction of H. pluvialis sequences was classified as genes involved in photosynthesis ($9\%$) and cellular process ($9\%$). Based on this EST analysis, the full-length cDNA sequence for superoxide dismutase (SOD) of H. pluvialis was cloned, and the expression of this gene was investigated. The abundance of SOD changed substantially in response to different culture conditions, indicating the possible regulation of this gene in H. pluvialis.

• BMB Reports
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• 제43권1호
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• pp.9-16
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• 2010

The promoters of OsCaM1 and OsCaM3 were characterized after sequencing and fused to the reporter gene, GUS. The constructs were then transformed into the tobacco plant. Histochemical analysis of GUS showed different expression patterns in pOsCaM1::GUS and pOsCaM3:: GUS transgenic plants. The expression of pOsCaM1::GUS in 4- to 15-day-old seedlings in particular was observed only in the root, while the expression of pOsCaM3::GUS was detected in both the cotyledons and root. Also, pRCaM1::GUS was detected in all the tissues surrounding the root system, while the presence of pOsCaM3::GUS was observed in the root, except in the root meristem. However, in mature transgenic plants, the expression of pOsCaM1::GUS and OsRCaM3::GUS was scarcely detected. Under wounding stress, the GUS activity of pOsCaM1 and pOsCaM3 was strongly induced, and the activity of pOsCaM3 especially, was retained for long periods. In the phloem, pOsCaM3 activity induced by hormone treatments and abiotic stresses was also identified.

• BMB Reports
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• 제53권4호
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• pp.206-211
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• 2020

Vascular smooth muscle cells (VSMCs) are a unique cell type that has unusual plasticity controlled by environmental stimuli. As an abnormal increase of VSMC proliferation is associated with various vascular diseases, tight regulation of VSMC phenotypes is essential for maintaining vascular homeostasis. Hypoxia is one environmental stress that stimulates VSMC proliferation. Emerging evidence has indicated that microRNAs (miRNAs) are critical regulators in the hypoxic responses of VSMCs. Therefore, we previously investigated miRNAs modulated by hypoxia in VSMCs and found that miR-1260b is one of the most upregulated miRNAs under hypoxia. However, the mechanism that underlies the regulation of VSMCs via miR-1260b in response to hypoxia has not been explored. Here we demonstrated that hypoxia-induced miR-1260b promotes VSMC proliferation. We also identified growth differentiation factor 11 (GDF11), a member of the TGF-β superfamily, as a novel target of miR-1260b. miR-1260b directly targets the 3'UTR of GDF11. Downregulation of GDF11 inhibited Smad signaling and consequently enhanced the proliferation of VSMCs. Our findings suggest that miR-1260b-mediated GDF11-Smad-dependent signaling is an essential regulatory mechanism in the proliferation of VSMCs, and this axis is modulated by hypoxia to promote abnormal VSMC proliferation. Therefore, our study unveils a novel function of miR-1260b in the pathological proliferation of VSMCs under hypoxia.

• 대한조선학회논문집
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• 제48권4호
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• pp.299-307
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• 2011

The offshore plants such as FPSO are subjected to combination loading of environmental conditions (swell, wave, wind and current). Therefore the fatigue damage is occurred in the operation time because the units encounter the environmental phenomena and the structural configurations are complicated. This paper is a research for frequency domain fatigue analysis of wide-band random loading focused on accuracy of fatigue damage estimation regarding the proposed methods. We selected ideal bi-modal spectrum. And comparison between time-domain fatigue analysis and frequency-domain fatigue analyses are conducted through the fatigue damage ratio. Fatigue damage ratios according to Vanmarcke's bandwidth parameter are founded for wide-band. Considering safety, we recommend that Jiao-Moan and Tovo-Benasciutti methods are optimal way at the fatigue design for wide-band response. But, it is important that these methods based on frequency-domain unstably change the accuracy according to the material parameter of S-N curve. This study will be background and guidance for the new frequency-domain fatigue analysis development in the future.

• 한국작물학회지
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• 제49권2호
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• pp.148-153
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• 2004

The Hessian fly, Mayetiola destructor (Say), is known to be one of the major insect herbivores of wheat worldwide. In order to provide molecular events on interactions of the NIL with H21 and larvae of Hessian fly biotype L, the TaCR1 gene, Triticum aestivum cytokinin repressed 1, was isolated through the suppression subtractive hybridization, which was constructed using stems of the NIL with H21 at 6 days after infestation as tester and stems of the recurrent parent Coker797 without H21 at 6 days after infestation as driver. Transcript levels of TaCR1 mRNA in the NIL with H21 were highest at 6 days after infestation but in the Coker797 without H21 until 8 days were similar with those of non-infested plants. Expression of the TaCR1 gene was decreased at early time and then recovered after wounding or $H_2O$$_2$ treatment as well as 6-BAP treatment. Transcripts levels of the TaCR1 gene was changed after MeJA, SA, ethephone, or ABA treatment. In drought treatment, the TaCRl gene were increased at early stage of stress and then decreased at late stage. Expression of the TaCRl gene was continued to decrease through 24 h in the cold treatment. Although the TaCRl gene is increased through infestation in NIL with H21, further study was required to elucidate a role on resistance against larvae of Hessian fly. However, the TaCR1 gene could be used as marker gene on response of plants against abiotic stresses as well as application of plants with several hormones.

• 한국지반환경공학회 논문집
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• 제23권5호
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• pp.5-13
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• 2022

본 연구에서는 국내 지진모의시험 수행 시 모래지반의 모사를 위해 국내에서 널리 이용되는 지반재료인 인공규사를 대상으로 상대밀도 차이에 따른 진동삼축시험을 수행하여 인공규사의 동적특성 및 액상화 저항특성을 실험적으로 분석하였다. 상대밀도 조건을 40%, 60%, 80%로 달리한 시험결과를 통해 상대밀도에 따른 액상화 저항특성을 확인하였으며, 액상화 진동저항응력비(Cyclic resistance stress ratio, CRR) 산정 곡선을 도시하였다. 또한, 액상화 진동저항응력비(CRR) 산정곡선의 타당성을 검토하기 위해 실내진동삼축시험에 사용된 규사로 이루어진 인공지반을 조성한 후, 간편예측법과 상세예측법을 통한 액상화 평가를 수행하여 각각의 안전율을 비교·검토하였다. 최종적으로, 상대밀도를 달리한 실험을 통해 인공규사의 동적물성을 파악하여 결과를 도출하였으며, 이 결과를 물성치로 적용한 상대밀도 40% 간편예측법은 안전율이 1로 안전한 지반으로 도출된 반면 상세예측법은 0.05작은 값으로 결과가 도출되었다.

• 한국환경과학회지
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• 제30권11호
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• pp.957-965
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• 2021

This research was carried out to investigate seed germination and growth of the perennial plant Apocynum lancifolium under different NaCl concentrations, with a view for future cultivation on reclaimed land. Initial characterization revealed that the average length and weight of A. lancifolium seed pods was 133.6 mm and 0.23 g, respectively, and the thousand-grain weight was 0.59 g. Upon examining the effects of light on seed germination, we found germination to be 1.7% higher under light conditions (90%) than under dark conditions (88.3%). In terms of the response to salt stress, we found that 90% of seeds germinated in the 0.00%, 0.25%, and 0.50% salt treatment groups. Although salt treatment up to a concentration of 0.5% was found to have little effect on seed germination, the rate of germination decreased at higher concentrations and was completely inhibited in the 2% treatment. We also established that germination rates were higher in seeds sown in horticultural topsoil than in the coarse sandy soil found in the plant's natural habitats. Although the growth of A. lancifolium tends to decrease with an increase in salt concentration, we found that the stem thickness, fresh weight, and dry weight of A. lancifolium seedlings subjected to 0.25%-1.0% salt were comparable to those of the control seedling that were not exposed to salt. Furthermore, in contrast to those plants subjected to 2.0% salt, these plants continued to grow and remained viable.

• Computers and Concrete
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• 제31권5호
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• pp.419-432
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• 2023

Beam-column joints are a critical component of reinforced concrete frame structures. They are responsible for transferring forces between adjoining beams and columns while limiting story drifts and maintaining structural integrity. During severe loading, beam-column joints deform significantly, affecting, and sometimes governing, the overall response of frame structures. While most failure modes for beam and column elements are commonly considered in plastic-hinge-based global frame analyses, the beam-column joint failure modes, such as concrete shear and reinforcement bond slip, are frequently omitted. One reason for this is the dearth of published guidance on what type of hinges to use, how to derive the joint hinge properties, and where to place these hinges. Many beam-column joint models are available in literature but their adoption by practicing structural engineers has been limited due to their complex nature and lack of practical application tools. The objective of this study is to provide a comparative review of the available beam-column joint models and present a practical joint modeling approach for integration into commonly used global frame analysis software. The presented modeling approach uses rotational spring models and is capable of modeling both interior and exterior joints with or without transverse reinforcement. A spreadsheet tool is also developed to execute the mathematical calculations and derive the shear stress-strain and moment-rotation curves ready for inputting into the global frame analysis. The application of the approach is presented by modeling a beam column joint specimen which was tested experimentally. Important modeling considerations are also presented to assist practitioners in properly modeling beam-column joints in frame analyses.