• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
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• pp.120-122
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• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• 한국전통조경학회지
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• 제32권4호
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• pp.120-131
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• 2014

조선 개국 시 영조된 사직단은 일제강점기를 거치면서 사직공원으로 고착화되었고, 최근 복원에 대한 노력이 진행되고 있다. 통시적으로 분석된 사직단의 변화 내용은 다음과 같다. 첫째, 한 나라의 중요 국가제사를 지내던 장소로의 입지를 확보하고 강화되었던 조선시대 태조에서 일제강점기 이전까지의 시기이다. 태조대에 인왕산 자락에 축조했으며 임진왜란 시 소실된 후 영조 대에 제례시설이 완비되었다. 그러나 1908년 일제의 간섭이본격화되자 공간 멸실과 제사가 폐지되었다. 둘째, 일제에 의해 공원화가 진행되어 고착화된 1910년부터 1944년까지이다. 공원 관련 시설과 경계부에 이질적인 건축물이 들어섰고, 단 영역 재실 사직단문이 고적과 보물로 지정되기도 하였다. 셋째, 해방 이후 1984년까지 이질적 성격의 동상과 기념비, 건축물이 축조되어 혼재된 장소성을 지니게 된 시기이다. 사직터널의 개설로 사직단 문이 2번에 걸쳐서 이축되기도 하였다. 넷째, 사직단의 역사성과 상징성을 회복하기 위한 노력이 진행되고 있는 1985년부터 현재까지이다. 사직단 권역의 복원 계획이 진행되고 있으나 주민에 대한 반발로 난항을 겪고 있는 시기이기도 하다. 향후, 사직단의 원형 회복을 위한 복원을 위해서는 발굴조사를 통한 면밀한 고증과 주민의 이해가 함께 진행되어야 한다.

• Toxicological Research
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• 제34권3호
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• 보건의료산업학회지
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• 제1권1호
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• pp.59-74
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• 2007

Considering the characteristics of the Korean family which maintain a close connection with their patients from the moment of their falling sick to hospitalization to discharge, the family is the most important environmental factor of the social supporting system, and is the important object of the client as well as activity system. The medical social work intends to meet the practical needs of aged chronic patients, providing them and their family with a professional human service. The end of this study is to find out the hardships of both the aged chronic patients and their family as well as their needs for the social welfare service, and to search out the way of comprehensive social work service. The summary of the analysis of the survey is as follows: 1. The needs of aged chronic patients are divided into those of the solution of the problems of falling ill, social welfare program and discharge. Those needs arc affected by the various factors of the types of hospitals, the patients' age, the kinds of insurance, and the supporting systems, etc. Accordingly, the assessment of the needs of the patients are asked to be done comprehensively in accordance with the kinds of diseases and social environments. 2. The importance of the family to the aged chronic patients is evident. The family plays a decisive role in the patients' hospitalization and discharge, the family being an important supporting system and making it necessary to take an approach to client system. The family has difficulty in getting connection of community resources, in adapting to social life after the patient's discharge, and in paying the treatment. The family suffers the secondary hardships more than the burden of the treatment expenses. 3. For this reason various interventions are needed to reduce the stress caused by supporting and nursing patients. Thus the social welfare service for the aged chronic patients and their family needs the following prepositions: 1. It is the characteristics of the aged chronic patients that they need continuous care and that the strengths of the patients and their family cannot be too much emphasized, and that comprehensive assessment based on the connection 'with the community and the mutual interchange 'with the environment, is much emphasized. 2. The family of the aged chronic patient is a resources system as well as a client one. 3. Another characteristic of the aged chronic patients is that with the resources connection in mind, it needs an active intervention of social workers in the community. With these prepositions considered, the development of practical social work service for the aged chronic patients is thought urgently needed.

• 한국화재소방학회논문지
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• 제27권3호
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• pp.85-93
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• 2013

본 연구에서는 소방대원이 수행하는 업무 중 중량물을 다루어야 하는 작업에 대한 분류 및 정량적 분석(NLE, 3DSSPP)을 수행하여 향후 소방대원의 중량물 관련 작업에 대한 위험도를 예측하고, 관련 위험도를 감소시키기 위한 기초 자료를 도출하고자 하였다. 도출된 위험도 등급은 위험도 점수가 3점부터 9점까지 총 7단계로 구성되었다. 가장 위험도가 높은 1등급은 나타나지 않았으며 인명구조 작업이 두 번째로 높은 2등급에 속하는 것으로 나타났다. 3등급은 파괴기구 활용, 환자 들어올리기작업 등으로 나타났다. 본 연구 결과들은 소방대원의 허리 부상을 포함하는 근골격계 질환 예방을 위한 작업방법 및 장비의 개선, 교육 프로그램의 개발에 기본 토대가 될 수 있다고 판단되며, 소방대원들의 근골격계 질환에 대한 지속적인 예방을 위해서는 본 연구의 골격을 토대로 한 추후 심층적인 조사와 연구가 시급하다고 사료된다.

• 자원ㆍ환경경제연구
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• 제16권2호
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• pp.275-311
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• 2007

수소에너지는 비고갈성 청정에너지로 향후 탄소기반의 에너지 시스템을 대체할 에너지로 주목받고 있다. 정부의 장기적인 비전도 수소에너지의 최종에너지 수요 비중을 2040년까지 15%로 확대한다는 것이나 이를 달성하기 위해서는 200조 원이 넘는 엄청난 비용이 수반된다. 본 연구는 동태 CGE 모형을 적용하여 수소에너지 보급에 따라 한국경제에 어떤 영향을 미칠 것인지를 분석하였다. 프런티어 기술로서 수소에너지는 학습효과와 에너지원간 상보성(complementarity)의 영향으로 정부지원 없이는 2040년 기준으로 최종에너지 수요의 6.5%에 그칠 전망이나 정부가 수소에너지에 대해 각각 10%, 20%, 30%의 가격보조 정책을 실시할 경우 9.2%, 15.2%, 37.7%로 증가할 것으로 분석되었다. 즉, 정부개업을 통해 수소기술 확산 지연 현상이 크게 완화될 수 있음을 보여주는 것이다. 한편 수소기술의 주요 응용분야인 수송부문의 성장이 두드러지나 수송 연료의 대부분을 차지하는 석유가 수소에너지로 대체됨으로써 석유부문에 대한 소비가 감소하고, 발전부문의 비중도 감소하는 것으로 나타났다. 정부의 가격보조가 가계소득에서 충당되는 것으로 가정했기 때문에 가격하락에 의한 소비증가효과가 소득감소에 따른 소비감소에 의해 상쇄되면서 가계의 최종소비도 부정적 영향을 받는 것으로 분석되었다. 그러나 전반적인 생산, 투자, 수출 증가에 힘입어 GDP 수준은 소폭 성장하다가 2040년 무렵에 소폭 감소하여 경제 전반에 미치는 영향은 미미할 것으로 분석되었다.

• 대한지역사회영양학회지
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• 제13권1호
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• pp.80-90
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• 2008

Atopic dennatitis (AD) is one of the major public health problem. It has been reported that the prevalence of AD in children and adults are 10-20% and 1-3%, respectively. Westernization of food habits, urbanization, and environmental pollution are contributing factors toward the recent rise in prevalence. Excessive dietary restriction leads to chronic malnutrition in atopic dermatitis patients. The purpose of this study was to investigate the effects of medical nutrition therapy (MNT) on quality of diet and blood immune parameters in atopic dermatitis patients. The 19 atopic dermatitis patients (7 men and 12 women) admitted to K University Medical Center were studied. During the 12 weeks of intervention, the subjects were given MNT by a dietitian for 30-45 minutes every other week. MNT was comprised with general dietary therapy, intake of balanced meals, emphasis on n-3 fatty acid contents in foods, and food allergies. Anthropometric and dietary assessment and blood analysis were taken at baseline and after 12 weeks of MNT. After 12 weeks of MNT, the subjects' dietary qualities, including dietary diversity score (DDS), meal balance score (MBS) and dietary variety score (DVS) were significantly increased (p < 0.05). According to significantly increased intake of EPA and DHA, dietary n-6/n-3 fatty acid ratio decreased to the recommended level for the atopic dermatitis patients (p < 0.05). These changes of dietary fatty acid consumption were reflected erythrocyte fatty acid composition. After 12 weeks of MNT, serum levels of IgE and IL-4 levels were significantly decreased, however, the levers of INF-$\{gamma}$, WBC, lymphocyte and TLC were not changed. As a conclusion, the individualized MNT improved the quality of diet in atopic dermatitis patients thereby influenced RBC fatty acid composition and IgE and IL-4 levels.

• 보건교육건강증진학회지
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• 제25권2호
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• pp.73-89
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• 2008

Objectives: This research was conducted to suggest a recommendation for the Korean credentialing policy of health education specialist as the primary human resource in community health promotion activities from the special group perspective of the Korean Society for Health Education and Promotion. Methods: This research was conducted by the professional focus group discussion and descriptive literature review on health education and promotion. Results: This draft recommendation for Korean credentialing system development of health education specialist was based on the four background reasons for modifying health promotion related acts, for developing better policy of health education credentialing, for keeping the public and ethical responsibilities as the competitive professional society, and for improving health promotion activities in Korea. Theoretical background of the four reasons was Ottawa Charter. We classified three credentialing levels of health education specialist based on health education own competencies, coordiating competencies with environmental factors, and research competencies. Furthermore, we developed 10 major roles and categorized 53 sub-roles based on these competencies above. We recommended 10 classes required to take to become Health Education Specialist. These 10 classes were developed based on the credentialing systems in the United States and Japan. These 10 classes were about health education and promotion methods and strategies not health intervention topics. We also built the draft plan for continuing education to keep KCHES based on the NCHEC in the United States. Conclusions: Further research should be conducted to build better health education specialist credentialing systems modifing current communtiy-based health promotion activities in terms of modifying public regulation, developing KCHEC examination system, protecting job security both in public and private sectors, and creating professionalism in KCHEC.

• 한국통신학회논문지
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• 제39C권9호
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• pp.828-840
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• 2014

사물을 인터넷에 연결시키는 기술인 Internet of Things(IoT)는 새로운 연구분야가 되어 기술적으로 많은 발전이 이루어져왔다. 이러한 IoT 기술은 사물을 관찰하거나 다루는데 있어 쉽게 접근하도록 하여 네트워크 상에서 인터넷으로 데이터의 전달을 가능하게 한다. 이는 두가지의 인프라를 필요로 하는데 하나는 데이터를 수집하는 Sensing Entity이고 다른 하나는 데이터를 점유하는 Sensor Cloud이다. 여기서 무선 센서 네트워크는 사물로부터 측정되는 데이터를 인터넷에 연결시키는데 매우 중요한 역할을 하는데 간단한 컴퓨팅 디바이스나 Arduino, Raspberry Pi와 같은 임베디드시스템으로 구성될 수 있다. Sensor Cloud는 사물로부터 측정되어 나오는 데이터의 공유나 처리를 하도록 하는 클라우드 컴퓨팅으로 모니터링 혹은 컨트롤 시스템에 대한 플랫폼을 제공한다. 본 논문에서는 의약품의 저장 조건들을 모니터링 하는 Cold Chain을 제안한다. 제안된 Cold Chain은 생산에서 소비단계까지 이르는 이동이나 저장상태에 대한 여러 환경적 조건인 습도, 온도, 저장 장소 등을 모니터링 하는 시스템이다. 시스템은 두개의 부시스템으로 구성되는데 하나는 이동에 관련된 것을 다루고 다른 하나는 저장에 관련된 것을 다룬다. 이 두 개의 시스템은 보다 향상된 모니터링 방법을 제공하기 위해 Sensor Cloud 시스템으로 통합된다.

• Elastomers and Composites
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• 제44권3호
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• pp.196-208
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• 2009

감응 성질을 탑재한 복잡한 고분자 재료가 시장에 등장하기 시작하고 있다. 가역적이며 비공유성인 상호작용을 사용하여 이와 같은 감응형 재료를 설계하는 예가 많아지고 있다. 최근에 개발된 수소결합 단위가 이와 같은 설계 기법을 극으로 치닫게 하고 있다. 단량체를 이어주는 힘이 수소결합뿐인 초분자 고분자는 온도나 용매의 변화에 기계적 성질이 감응되는 재료를 형성한다. 본 보문에서는 수소결합으로 이루어진 초분자 고분자가 자기치유 재료로 사용되는 예를 소개한다. 재생 가능한 고무성 재료를 합성하는 일은 그리 매력적이라 할 수 없다. 그러나 상온에서 접착제를 사용하지 않고도 손상을 반복해서 치유할 수 있는 고무 재료는 우리의 상상의 나래를 펼치게 하는 재료이다. 외부의 간섭 없이 환경적 자극에 비선형적으로 감응하여 파손된 부분을 치유시키는 재료인 자기치유 재료는 여러 가지 첨단공학 계에서 엄청난 가능성을 가진 재료라 할 수 있다.