• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2002년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.216-217
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• 2002

Vitellogenin (VTG) is a Precursor form of egg yolk Proteins, which appear only in the blood circulation of female fish and its synthesis in the liver is considered to be regulated by several hormones. It has been reported that in addition to estradiol-17 $\beta$ (E2) several hormones are also involved in the production site of VTG, the liver (Peyon et al., 1996; Mori et al., 1998). (omitted)

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.117-132
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• 1987

Plants are inherited spatial and temporal coordination systems in their growth and differentiation processes which are precisely governed by the two interlocked control systems; autogenous and environmental. Looking into the overall course of plant development from molecular to organismal level, it can be comparable to a concerto for plant hormones, environmental stimuli and plant genomic orchestra conducted by an unidentified virtuoso. Some of the recent significant attempts to puzzle out the mystery of the life processes of plant development are briefly reviewed. The revolutionary advances in understanding the mystic processes are contemporarily achieved by the application of various molecular techniques. The characterization of plant genomes is now attained through recombinant DNA approaches, and the sensitive detection of specific gene products during the plant development is perimitted by the immunochemical procedures. However, along with the recognition of underlying molecular events such as developmental changes in gene expression and hormone-receptor interrelation associated with tissue sensitivity to hormones, more emphasis should be placed upon the physiological approaches of organismal level for the understanding the correlative systems of the developmental processes of plants as intact eukaryotic organisms.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.293-298
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• 2021

Di(2-ethylhexyl)phthalate (DEHP) could induce metabolic disorders through interfering with thyroid homeostasis. Therefore, we evaluated the effects of short term to environmental relevant doses of DEHP on thyroid hormones. Four week old Sprague-Dawley (SD) rats were treated with vehicle (corn oil), and DEHP 0.75, 7.5, and 150 mg/kg/day. The rats were treated with once daily by oral gavage and were sacrificed with after 1 week. They were measured body weight and relative thyroid weight, serum thyroid hormones and histological changes of thyroid. There was no difference in body weight between the control and DEHP exposed rats. Relative thyroid weight in DEHP 150 mg/kg/day treated group was significantly lower than control. Serum thyroxine levels was decreased in rats exposed to 0.75 and 150 mg/kg/day DEHP. No histological changes were observed in the thyroid of rats administered DEHP compared to control. Exposure to DEHP at environmental relevant levels, even short-term exposure, can cause hypothyroidism in adolescent rats even the exposure period is relative short.

• Toxicological Research
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• 제21권4호
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• pp.333-338
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• 2005

Many of thyroid hormones disrupting chemicals induce effects via interaction with thyroid hormone and retinoic acid receptors and responsive elements intrinsic in target cells. We studied thyroid hormones disrupting effects of food additives and contaminants including BHA, BHT, ethoxyquin, propionic acid, sorbic acid, benzoic acid, CPM, aflatoxin B1, cadmium chloride, genistein, TCDD, PCBs and TDBE in recombinant HeLa cells containing plasmid construct for thyroxin responsive elements. The limit of response of the recombinant cells to T3 and T4 was $1\times10^{-12}\;M$. BHA. genistein, cadmium and TBDE were interacted with thyroid receptors with dose-responsive pattern. In addition, BHA, BHT, ethoxyquin, propionic acid, benzoic acid, sorbic acid, and TBDE showed synergism while cadmium chloride antagonism for T3-induced activity. This study elucidates that recombinant HeLa cell is sensitive and high-throughput system for the detection of chemicals that induce thyroid hormonal disruption via thyroid hormone receptors and responsive elements. Also this study raised suspect of BHA. BHT, ethoxyquin, propionic acid, benzoic acid, sorbic acid, TBDE, genisteine and cadmium chloride as thyroid hormonal system disruptors.

• 한국환경과학회지
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• 제2권4호
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• pp.279-289
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• 1993

자연광 및 여러 파장의 광선과 NAA, GA$_3$, BA 등의 식물호르몬을 옥수수 유식물에 처리하여 엽록소-단백질 복합체 형성에 미치는 효과를 조사하였다. 자연광하에서 3 종류의 호르몬 처리는 전체적으로 엽록소-단백질 복합체, 특히 LHCP-1과 LHCP-3의 형성을 촉진하였으나, 2 종류의 호르몬 조합은 엽록소-단백질 형성에 효과적이지 못하였다. LHCP-1, CPA 및 LHCP-3 등의 광계 II 관련 엽록소 형성에 있어서 백색광은 자연광에 비하여 효과가 적었으나 적색광은 효과적이었다. 적색광하에서 식물호르몬의 단독처리는 엽록체 발달 초기에 광계 II의 엽록소-단백질의 양적 증가를 유도하였다. 한편, 적색광하에서 2 종류의 호르몬 조합 처리도 자연광하에서의 처리와는 대조적으로 광계 II의 엽록소-단백질 형성에 매우 효과적이었다. 이와 같은 결과는 광선처리의 효과가 호르몬처리 효과보다 엽록소-단백질 복합체 형성에 중요한 인자로 작용한다는 것을 시사해 준다.

• 한국식품영양과학회지
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• 제38권6호
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• pp.663-666
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• 2009

산수유 에탄올 추출물이 환경호르몬인 dioxin 및 bisphenol A(BPA)에 의해 유도된 인체 전립선 암세포인 RC58T/h/SA#4(prostate epithelial primary cancer cell lines)의 성장을 억제하는지를 조사하였다. 즉 산수유 에탄올 추출물을 10, 100, 300 및 $500{\mu}g/mL$ 농도로 RC58T/h/SA#4에 처리했을 시 $500{\mu}g/mL$ 농도 이상에서 높은 성장 억제율을 나타내었으며, dioxin과 bisphenol A를 다양한 농도로 처리한 결과 각각 1 nM, $0.1{\mu}M$ 농도에서 가장 높은 전립선암세포 증식을 유도하였다. 한편 환경호르몬(dioxin, BPA)에 의해 RC58T/h/SA#4의 증식을 유도시킨 후 산수유 에탄올 추출물을 10, 100, 300 및 $500{\mu}g/mL$ 농도로 처리한 결과 대조군에 비하여 산수유 에탄올 추출물 $500{\mu}g/mL$ 농도에서 그 억제율이 가장 높았다.

• 한국환경과학회지
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• 제15권12호
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• pp.1193-1197
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• 2006

This study was to examine the variations of chromosome number and the ranges of variety in the suspension culture of ginseng (Panax ginseng C. A. Meyer) callus cell, and the effect of plant hormones for the chromosome aberration. Plant hormones added with MS medium in the suspension culture were 2,4-D, kinetin, and 2,4-D+kinetin and concentration of the plant hormones were $1000{\mu}M$ and $0.1\;{\mu}M$ respectively. As a result of these experiment the following conclusion has been obtained. Media contained with 2,4-D+kinetin in $10{\mu}M$ concentration was very effective in the suspension culture result from 26.4% mitosis frequency, and found the various variation of chromosome number. Variety of chromosome number was diversed ($9\sim110$), espicially frequency of hypohaploid and hyperhaploid cells were very higher than hyperdiploid cells. In this experiments, it is suggested that $10{\mu}M$ 2,4-D+kinetin added with medium in the suspension culture of ginseng callus was effect in the variations of chromosome number.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2002년도 학술대회
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• pp.33-37
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• 2002

The present was performed to identify the effects of tributyltin (TBT) in the immature mice testes. 3-week-old male ICR mice were orally administrated on one time basis of TBT dose of O (Vehicle control, VC), 25 (TBT 25 mg/kg, T$_{25}$ ), 50 (TBT 50 mg/kg, T$_{50}$ ), 100 (TBT 100 mg/kg, T$_{100}$ ) mg/kg per each one. After 3 days the time treated of TBT, mice were sacrified and wighted body, testis, epididymis, seminal vesicle, vas deferens, and prostate. As the result of weighing, wights of each oragan and gonad index were tendency decresed in comparing groups of TBT treated with that (C) of unteated (p <0.05). As the result of examination of steroid hormones in the immature male mice, The concentrations of serum and intratesticular testosterone were significatly increased rather than the control group. But concentrations of estradiol were decresed objectly. A group of the highest change of concentrations of steroid hormones is T$_{100}$ . The high dose group, T$_{100}$ , was decreased all of concentrations of steroid hormones rather than those of T$^{25}$ . The result of observation with histological changes in testis showed a tendency for innercellular wall to increase damage and extinction in seminiferous tubles. As the result of investigation apoptotic cell numbers in the testis using teminal deoxy-nucleotidyl transferase -mediated dUTP-digoxygenin nick end-labeling immunohistochemical straia, The ratio of Apoptic cells significantly was incensed in depending on treatment of TBT does. In conclusion, these results shows that TBT triggers apoptosis on reproductive cell in testis and changes level of concentrations of steroid hormones in the immature male mice , as endocrine disruptors (EDs).

• 환경생물
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• 제21권4호
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• pp.352-357
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• 2003

Phenanthrene, one of Polycyclic aromatic hydrocarbons with three aromatic rings, is a ubiquitous contaminant in the environment. Phenanthrene has been identified in ambient air, drinking water and sediment. We examined the effect of phenanthrene on steroid hormones level of olive flounder, Paralichthys olivaceus. Plasma testosterone level was increased significantly in fish exposed to phenanthrene($\geq1.0 \mu M$) at 4th week. However, there was no significant changes of estradiol-17$\beta$ concentration in fish exposed to phenanthrene. The physiological variation in phenanthrene exposed fish was a dramatic increase in plasma cortisol level. It is concluded that chronic exposure of phenanthrene can induce increase of plasma testosterone levels and elevate the plasma cortisol level in flounder, Paralichthys olivaceus.

• 통상정보연구
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• 제13권3호
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• pp.403-431
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• 2011

환경 관련 문제에 대한 관심이 고조됨에 따라, 국민의 건강 및 생명 보호를 위한 각국의 SPS조치는 국가 간 자유무역이라는 WTO의 주요원칙에 역행하는 규제적인 무역관행을 낳고 있다. 그러나 분쟁 발생 시 패널이 회원국의 조치를 심사하는데 필요한 심사기준과 관련된 규정은 SPS협정에 존재하지 않는다. 뿐만 아니라 과학적 실험, 데이터 등과 밀접한 관련이 있는 협정의 특성으로 인하여, 본 협정에 적합한 심사기준에 대해서는 상반된 의견들이 대립하고 있으며 실제 분쟁사례에서도 일관된 기준이 적용되고 있지 못하다. 국가차원에서 결론 내려진 사실적, 법적 판단을 패널이 심사할 때 적용하는 심사기준은, 당국의 결정을 패널이 존중하는 정도에 따라 '적극적 개입(de novo review) 기준'과 '존중(deference) 기준'이라는 두 가지의 대립적인 기준으로 나누어질 수 있다, 이 두 가지 대립적인 기준을 중심으로, 본 논문에서는 WTO협정에서 규정하고 있는 심사기준을 검토하고 SPS 협정에 적합하게 적용할 수 있는 심사기준을 모색하고자 한다. 그리고 최근 US-Hormones Suspension 사건 판결에서 적용한 심사기준을 분석하여 SPS협정에 적용 가능한 심사기준이 어떠한 방향으로 정립되고 있는지 살펴보고자 한다.