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http://dx.doi.org/10.5322/JES.2006.15.12.1193

Chromosome Aberration in Suspension Culture of Ginseng(Panax ginseng C. A. Meyer) Callus  

Park, Jong-Bum (Department of Biological Sciences, Silla University)
Publication Information
Journal of Environmental Science International / v.15, no.12, 2006 , pp. 1193-1197 More about this Journal
Abstract
This study was to examine the variations of chromosome number and the ranges of variety in the suspension culture of ginseng (Panax ginseng C. A. Meyer) callus cell, and the effect of plant hormones for the chromosome aberration. Plant hormones added with MS medium in the suspension culture were 2,4-D, kinetin, and 2,4-D+kinetin and concentration of the plant hormones were $1000{\mu}M$ and $0.1\;{\mu}M$ respectively. As a result of these experiment the following conclusion has been obtained. Media contained with 2,4-D+kinetin in $10{\mu}M$ concentration was very effective in the suspension culture result from 26.4% mitosis frequency, and found the various variation of chromosome number. Variety of chromosome number was diversed ($9\sim110$), espicially frequency of hypohaploid and hyperhaploid cells were very higher than hyperdiploid cells. In this experiments, it is suggested that $10{\mu}M$ 2,4-D+kinetin added with medium in the suspension culture of ginseng callus was effect in the variations of chromosome number.
Keywords
Chromosome aberration; Suspension culture; Ginseng; Callus;
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1 Prasad, G. and K. Das, 1977, Effects of some growth substances on mitosis, Cytologia, 42, 323-329   DOI   ScienceOn
2 Vig, B. K., 1969, Relationship between mitotic events and leaf spotting in Glycine max, Can. J. Genet. Cytol., 11, 147-152   DOI
3 Torrey, J. G., 1959, Experimental modification of development in the root, Symp. Soc. Study Develop. Growth, 17, 189-222
4 Venketeswaran, S., 1963, Tissue culture studies on Vicia faba, II. Cytology, Caryologia, 16, 91-100   DOI
5 Mitra, J. and F. C. Steward, 1961, Growth induction in cultures of Haploppus gracilis, II. The behaviour of the nucleus, Amer. J. Bot., 48, 358-368   DOI   ScienceOn
6 Singh, B. D. and B. L. Harvey, 1975, Cytogenetic studies on Haplopappus gracilis cells cultured on agar and in liquid media, Cytologia, 40, 347-354   DOI
7 Cooper, L. S., D. C. Cooper, A. C. Hildebrandt and A. J. Riker, 1964, Chromosome numbers in single cell clones of tobacco tissue, Amer. J. Bot., 31, 284-190
8 Shimada, T. and M. Tabata, 1967, Chromosome numbers in cultured pith tissue of tobacco, Jap. J. Genet., 42, 195-201   DOI
9 竹中要(Y. Takenaka), 1937, 人蔘細胞の染色体に關よろ硏究, 朝鮮博物誌, 22, 53-61
10 Lee, C. D. and S. S. Hong, 1980, Study on breeding of Korean ginseng (Panax ginseng C. A. Meyer), Sung Kyun Kwan Univesity Journal, 27, 1-11
11 Murashige, T. and R. Nakano, 1967, Chromosome complement as a determinant of the morphogenic potential of tobacco cells, Amer. J. Bot., 54(8), 963-970   DOI   ScienceOn
12 Kao, K. N., R. A. Miller, O. L. Gamborg and B. L. Harvey, 1970, Variations in chromosome number and structure in plant cells grown in suspension culture, Can. J. Genet. Cytol., 12, 297-301   DOI
13 Ogura, H., 1975, Morphactin-kinetin interaction on growth and shoot formation in tobacco callus cultures, Plant Physiol., 16, 563-569
14 Torrey, J. G., 1961, Kinetin as trigger for mitosis in mature endomitotic plant cells, Exper. Cell Res., 23, 281-299   DOI   ScienceOn
15 Lee, C. D. and S. S. Hong, 1978, Studies on callus cultures of medical plant in vitro, II. On the callus culture of Panax ginseng C. A. Meyer, Sung Kyun Kwan Univesity Journal Natural Science, 6, 5-11
16 Partanen, C. R., 1963, Plant tissue culture in relation to developmental cytology, Intern. Rev. Cytol., 15, 215-243   DOI
17 Sacristan, M. D., 1971, Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris Wallr., Chromosoma, 33, 273-283
18 DeTorok, D. and P. R. White, 1960, Cytological instability in tumor of Picea glaica, Cytochem., 17, 266-272
19 Torrey, J. G., J. Reinert and M. Merkel, 1962, Mitosis in suspension cultures of higher plant cells in a synthetic medium, Amer. J, Bot. 49, 420-425   DOI   ScienceOn
20 Heinz, D. J., G. W. P. Mee and L. G. Nickell, 1969, Chromosome number of some Saccharum species hybrids and their cell suspension cultures, Amer. J. Bot., 56(4), 450-456   DOI   ScienceOn
21 Masuda, Y., 1965, RNA in relation to the effect of auxin, kinetin and gibberellic acid on the tuber tissue of Jerusalem artichoke, Physiol. Plant., 18, 15-22   DOI
22 Nishiyama, I. and T. Taira, 1966, The effect of kinetin and indol acetic acid on callus growth and organ formation in two species of Nicotiana, Jap. J. Gentics., 41, 357-366   DOI
23 Yazawa, S., 1967, Cytological and morphological observations on the callus tissue of Crepis capillaris, Bot. Mag., 80, 413-420   DOI