• Journal of Food Hygiene and Safety
• /
• v.37 no.1
• /
• pp.29-37
• /
• 2022

This study will evaluate the effect of fermented Morinda citrifolia L. extracts and its marker compounds to provide baseline data for utilizing Morinda citrifolia L. as functional health products. Morinda citrifolia L. and six marker compounds were processed on RAW 246.7 macrophage to test for XTT Cytotoxicity, measure Nitric Oxide and Cyokine formation, and analyze the expression of immune marker genes. Furthermore, LPS and fermented red ginseng extract, a common functional ingredient, are used as positive controls. Our results showed that fermented Morinda citrifolia L and six bioactive compounds did not have any cytotoxic effect in all treatment concentrations and groups. Among six bioactive compounds, SCP and ASE confirmed the formation of NO. In addition, the ASE treatment group showed increased formation of IL-6 and IL-1β and the expression of iNOS and TNF-α. Also, fermented Morinda citrifolia L extract activated the macrophage by enhancing the production of nitric oxide (NO), interleukin (IL)-6, and IL-1β, and the expression of COX2 compared to Morinda citrifolia L. extracts. The result of the study showed that Fermented Morinda citrifolia L. (Noni) and marker compound enhance the innate immunity activity and suggested that the bioactive compound could be applied as a marker compound. Thus, Fermented Morinda citrifolia L. (Noni) could be used as functional food material to develop immunity-enhancing products, and highly functional marker compounds can be utilized as the effective components.

• Korean Journal of Environmental Agriculture
• /
• v.38 no.4
• /
• pp.307-313
• /
• 2019

BACKGROUND: An intensive farming system may be of the most important source for agricultural non-point source (NPS) pollution, which is a major concern for agricultural water management in South Korea. Various management practices have therefore been applied to reduce NPS loads from upland fields. This study presents performances of sediment trap for reducing NPS and soil loss from rainfall runoff in cropland. METHODS AND RESULTS: In 2018 and 2019, three sediment traps (L1.5 m × W1.0 m × D0.5 m = 0.75 ㎥) and their controls were established in the end of sloped (ca. 3%) upland field planted with maize crops. Over the seasons, runoff water was monitored, collected, and analyzed at every runoff. Soils deposited in sediment traps were collected and weighed at the season end. Sediment traps reduced runoff amount (p<0.05) and NPS concentrations, though the decreased NPS concentrations were not always statistically significant. In addition, sediment traps had a significant prevention effect on soil loss from rainfall runoff in a sloped cropland. CONCLUSION: The results suggest that the sediment trap could be a powerful and the best management practice to reduce NPS pollution and soil loss in a sloped upland field.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.6
• /
• pp.3805-3808
• /
• 2013

Background: Osteosarcomas have many established risk factors, both genetic and environmental, but by themselves these explain only part of the total cancer incidence. Bisphenol A (BPA) is an environmental estrogen associated with risk of several kinds of tumour. The lysyl oxidase gene (LOX) may also contribute to risk of tumours including osteosarcomas. Here, we investigated possible interactions of BPA and a LOX polymorphism on the risk of osteosarcoma. Method: The present hospital-based case-control study included 106 cancer patients and 112 controls from a Chinese population. Internal burden of BPA exposure was assessed using high-performance liquid chromatography-mass spectrometry (HPLC-MS) method. Genotypes were determined using PCR-RFLP methods. Results: Compared with those in low BPA exposure group, subjects with BPA more than or equal to median value had significant increased risk of osteosarcoma among subjects who carried GC or CC genotypes. A significant interaction with BPA level and the -22G/C polymorphism was observed for osteosarcoma overall, osteosarcoma affecting knee and osteosarcoma affecting hip, as $P_{forinteraction}$ = 0.036 for osteosarcoma overall; $P_{forinteraction}$ = 0.024 for osteosarcoma affecting knee; and $P_{forinteraction}$ = 0.017 for osteosarcoma affecting hip. Conclusions: The results suggest that BPA exposure interacts with the -22G/C polymorphism of the LOX gene to increase the risk of osteosarcoma.

• Environmental Mutagens and Carcinogens
• /
• v.23 no.3
• /
• pp.99-102
• /
• 2003

IARC classified areca quid as a human carcinogen. Areca quid chewed in Taiwan includes Piper betle inflorescence, which contains high concentrations of safrole (15 mg/fresh weight). Safrole is a documented rodent hepatocarcinogen, and chewing areca quid may contribute to human exposure (420 $\mu$m in saliva). The carcinogenicity of safrole is mediated through 1'-hydroxysafrole formation, followed by sulfonation to an unstable sulfate that reacts to form DNA adducts. Using human liver microsomes and Escherichia coli membranes expressing bicistronic human P450s, CYP2E1 and CYP2C9 were identified as the main P450s involved in the activation of safrole. We have demonstrated the presence of stable safrole-dGMP adducts in human oral tissues following areca quid chewing using $^{32}$ P-postlabeling and HPLC mass spectrometry methods. By studying 88 subjects with a known AQ chewing history and 161 matched controls, we have demonstrated that the presence of safrole-DNA adducts in peripheral blood cells was correlated to AQ chewing, and CYP2E1 seemed to play an important role in the modulation of safrole-DNA adduct formation. We have also shown that safrole can form stable safrole-DNA adducts as well as oxidative damages in rodent liver. However, the stable safrole-DNA adducts may represent a more significant initial lesion as compared to the rapidly repaired safrole-induced 8-hydroxy-2'-deoxyguanosine. This oxidative DNA damage is mediated through the formation of hydoryxchavicol, the major safrole metabolite in human urine. Hydroxychavicol may have gone through two-electron oxidation to the o-quinone; then via one-electron reduction to semiquinone radicals to generate oxidative DNA damage. However, these reactive metabolites can be efficiently conjugated by GSH. These data suggest that safrole may contribute to the initiation of oral carcinogenesis through safrole-DNA adduct and not oxidative DNA damage. In addition, CYP2E1 may modulate this adduct formation.

• Journal of Environmental Science International
• /
• v.18 no.5
• /
• pp.589-594
• /
• 2009

The goals of this study were conducted to investigate the effects of applying liquid aluminum chloride ($AlCl_3$) to rice hulls on pH and soluble reactive P (SRP). A total of 800 broiler chicks (4 treatments $\times$ 4 replicates $\times$ 50 birds) were housed into 16 floor pens in a single house for 5 weeks. The treatments were divided into 4 groups: control, 100 g of liquid $AlCl_3/kg$ of rice hulls, 200 g of liquid $AlCl_3/kg$ of rice hulls, and 300 g of liquid $AlCl_3/kg$ of rice hulls. Liquid $AlCl_3$ was sprayed on the rice hulls surface at a rate of 100 g, 200 g, and 300 g liquid $AlCl_3$ per kg rice hull. pH values and SRP contents were significantly decreased (P<0.05) with the increased liquid $AlCl_3$ levels in comparison with control. However, no significant differences in SRP contents were observed among all treatments at 3 and 4 weeks. Applying 100 g, 200 g, and 300 g liquid $AlCl_3$ to rice hulls reduced SRP contents by 18, 25, and 52% for 5 weeks, respectively, compared with the controls. In conclusion, these results suggest that using liquid $AlCl_3$ on rice hulls should be promising for reducing water contamination and resulted in a reduction in SRP contents, which reduced pH.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.25 no.3
• /
• pp.299-305
• /
• 2015

Global environmental concerns and the ever increasing need of energy, coupled with steady progress in renewable energy technologies, are opening up new opportunities for utilization of renewable energy resources. Distributed electricity generation is a suitable option for sustainable development thanks to the load management benefits and the opportunity to provide electricity to remote areas. Solar energy being easy to harness, non-polluting and never ending is one of the best renewable energy sources for electricity generation in present and future time. Due to the random and intermittent nature of solar source, PV plants require the adoption of an energy storage and management system to compensate fluctuations and to meet the energy demand during night hours. This paper presents an efficient, economic and technical model for the design of a MPPT based grid connected PV with battery storage and management system. This system satisfies the energy demand through the PV based battery energy storage system. The aim is to present PV-BES system design and management strategy to maximize the system performance and economic profitability. PV-BES (photovoltaic based battery energy storage) system is operated in different modes to verify the system feasibility. In case of excess energy (mode 1), Li-ion batteries are charged using CC-CV mechanism effectively controlled by fuzzy logic based PID control system whereas during the time of insufficient power from PV system (mode 2), batteries are used as backup to compensate the power shortage at load and likewise other modes for different scenarios. This operational mode change in PV-BES system is implemented by State flow chart technique based on SOC, DC bus voltages and solar Irradiance. Performance of the proposed PV-BES system is verified by some simulations study. Simulation results showed that proposed system can overcome the disturbance of external environmental changes, and controls the energy flow in efficient and economical way.

• Environmental Mutagens and Carcinogens
• /
• v.21 no.2
• /
• pp.128-134
• /
• 2001

Single cell gel electrophoresis (SCGE) assay, also called comet assay, is a rapid and sensitive method to detect DNA damage in single cell level. To evaluate the DNA damage of lymphocytes of pesticides sprayers, SCGE assay was carried out for 50 pesticides sprayer and 58 control subjects. They were interviewed with structured questionnaire to get the information about the kinds and amount of pesticide. Insecticides and fungicides were predominant among pesticides. Major components of pesticides were organophosphorus, organosulfate, cartap, carbamates, and triazole. Sprayed pesticides were classified into two groups. Group I included organophosphorus, organoarsenic, organotin, tetrazine, triazole and gramoxone, which were known to cause DNA damages. Group II pesticide were carbamates, surfactants, organosulfates, etc., which were not found as DNA damaging agents in scientific documents. Olive tail moments of 100 lymphocytes were measured by KOMET 3.1 program for each person. The means of tail moments were compared between farmers exposed to pesticides and control subjects. Farmers showed higher tail moments than control subjects (2.07$\pm$1.40 vs 1.53$\pm$0.77, p<0.05). The means of tail moments also were compared among group I sprayers (n=36), group II sprayers (n=24) and, control subject, and the means or tail moments were 3.4s$\pm$3.2o, 2.66$\pm$2.20 and 1.53$\pm$0.77 respectively. The difference between means of group I sprayers and controls was statistically significant (p<0.05). In conclusion, this study showed higher DNA damage in farmers exposed to pesticides than control subjects, and comet assay could be useful as a biological monitoring method of genotoxic pesticides for farmers.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.38 no.8
• /
• pp.657-663
• /
• 2014

Since 2007, the defect confirmation test for vehicles using PEMS has been enforced in USA. This test can measure emissions from on-street vehicles using a device mounted on a car. Europe has confirmed its plan for introducing this test from EURO6, 2013. Thus, the Korean government is also under pressure to adopt this method that reflects the real-world driving conditions using PEMS, considering the emission controls for domestic heavy-duty vehicles. To provide various utilizations of the PEM, this emission test has been developed in accordance with the type of driving road, DPF, ISG, and air conditioner. This research aims to provide the fundamental materials for implementing defect confirmation tests for commercial vehicles, which are appropriate for domestic emission control situations, after studying the defect confirmation test methods for heavy-duty vehicles using PEMS.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.8 no.5
• /
• pp.745-750
• /
• 2013

In many recent studies, a variety of environmental control system for practical gardening facilities such as facility house and plant factory have been proposed. However, the plants have been exposed to growth disorder and disease and pest injury because the temperature and humidity have not properly controlled so far. Therefore, a lot of damage of farmers have been reported. The air circulation fan and industrial dehumidifier have been currently utilized as the countermeasures, but they do not meet the expectation. In this study, the growth phase of each plant is recognized by using cycle-by-cycle plants growth recogniztion algorithm to provide optimal environment according to the growth phases of each plant.he productivity can be raised by using cycle-by-cycle plant growth recognition monitoring system because it optimally controls the environment by cycle that is required for plant growth.

• Environmental Mutagens and Carcinogens
• /
• v.18 no.1
• /
• pp.22-25
• /
• 1998

Anticarcinogenic activity of astaxanthin-containing egg yolks (designate AEY) was investigated for 7,12-dimethylbenz[a]anthracene (DMBA)-induced two stage mouse epidermal carcinogenesis. Female ICR mouse (6-7 weeks of age) were house in a humidity-and-temperature-controlled facility and subjected to feed and water ad libitum. AEY (10 mg/0.2 ml acetone) was painted on the back of mice 7 days, 3 days and 5 min before DMBA treatment (50 nmole/0.2 ml acetone). One week later after DMBA treatment, 6 ${\mu}g$ tetradecanoyl 12-phorbol 13-O-acetate (TPA) dissolved in 0.2 ml acetone was applied on the mouse twice weekly over a period of 22 weeks. No sample was given to control mice. Control egg yolk (CEY) and astaxanthin-containing oil (designate AO) from Phaffia rhodozyma were used as positive controls. Mouse treated with AEY exhibited 10 tumors per mouse whereas control mouse exhibited 15 tumors per mouse, the fact that 33% reduction of tumor per mouse by AEY treatment. Tumor incidence was also reduced to 15% by AEY treatment when compared to that of control group. Such effects were also seen in CEY and AO treatment groups, but leaser extent. AO gave reduction of food intake and body weights relative to those of AEY and CEY, indicating toxicity of AO. These results suggest that AEY exhibits anticarcinogenic activity for DMBA-induced mouse epidermal carcinogenesis.