• Title/Summary/Keyword: enteric bacteria

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The influence of various medicines and nutritional supplements against bacteria with gastrointestinal relevance (다양한 약물과 영양제가 장내세균에 미치는 영향)

  • Park, Jae Eun;Lee, Do Kyung;Ha, Nam Joo;Song, Young Cheon
    • Korean Journal of Microbiology
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    • v.51 no.1
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    • pp.61-67
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    • 2015
  • Recently, change of Western pattern diet and lifestyle is caused by various metabolic disorders and chronic diseases. These diseases need to take medicine regularly. Also, many people take health functional food, various vitamins and nutritional supplements in order to maintain a healthy life. But, there was no study about affects taking medicines against bacteria with gastrointestinal relevance. This study was performed by antibacterial activity test to evaluate the influence of a long time or commonly used medication. As a result, medicines of Vitamins & Minerals or Central nervous system show antibacterial activity against beneficial enteric bacteria and harmful enteric bacteria. Dexibuprofen of the Anti-inflammatory Drugs that acts on the central nervous system has shown high antibacterial activity at beneficial enteric bacteria strains (Lactobacillus casei, Lactobacillus rhamnosus) and harmful enteric bacteria (Staphylococcus aureus). Also, fenofibric acid of the antilipemic agents that acts on the Cardiovascular & Hematopoietic system has shown high antibacterial activity at beneficial enteric bacteria strains (Lactobacillus casei). Vitamins & Minerals appeared antibacterial activity against most intestinal bacteria. Vitamin B-Complex/with C and vitamin C were especially high with beneficial enteric bacteria strains (Bifidobacterium infantis) and harmful enteric bacteria (E. coli, E. aerogenes, S. flexneri, S. Typhimurium, S. aureus). Therefore, these results indicate that variously taking medicines have generally antibacterial activity against harmful enteric bacteria strains and beneficial enteric bacteria strains.

Distribution of Microflora in Powdered Raw Grains and Vegetables and Improvement of Hygienic Quality by Gamma Irradiation (유통 생식제품의 미생물 분포 및 감마선 조사를 이용한 위생화)

  • Kim, Dong-Ho;Song, Hyun-Pa;Yook, Hong-Sun;Chung, Young-Jin;Kim, Yeung-Ji;Byun, Myung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.4
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    • pp.589-593
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    • 2002
  • Improvement of hygienic quality of powdered raw grains and vegetables by gamma irradiation was investigated. Five products of powdered raw grains and vegetables were collected in a local market and analysed. The total viable cell counts of Bacillus were 10$^4$~10$^{7}$ cfu/g, filamentous fungi l0$^2$~10$^3$cfu/g, coliform 10$^1$~10$^4$cfu/g, enteric bacteria on SS agar plate 10$^1$~10$^3$cfu/g. Coliform, enteric bacteria on SS agar plate and filamentous fungi were eliminated by 3 kGy of gamma irradiation. The D values of coliform, enteric bacteria on SS agar plate, Bacillus, and filamentous fungi were 0.68~0.80 kGy, 0.59~0.74 kGy, 1.84~2.18 kGy and 0.36~0.57 kGy, respectively. It was considered that optimal irradiation dose for radappertization was about 25 kGy, while 3~5 kGy of gamma irradiation was effective for radicidation.

Colony Count with Mixed Culture of Enteric Bacteria by in vitro Quantitative Method (장내세균의 시간차 혼합배양이 보여주는 균수측정의 비교)

  • 황선철;전보성
    • Korean Journal of Microbiology
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    • v.11 no.4
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    • pp.175-180
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    • 1973
  • This study was attempted to see more clear relationships among the enterobacteria, especially between the intestinal normal flora and pathogenic bacteria. It has been known that some intestinal normal flora produce the bactrial metabolites that are harmful to other enteric bacteria. One of the metabolites is known as colicin, the protein fraction, which possesses certain degree of inhibitory effect against other bacterial growth fraction, whih possesses certain degree of inhibitory effect against other bacterial growth. As a preliminary study for a colicin purification, the antagonistic effect of E, coli to groups of Salmonella and Shigella has been studied by means of in vitro quantitative culture method. 1. E.coli showed definite inhibitory effects aganist both Salmonella and Shigella groups in the mixture of two organisms. 2. The inhibitory effects of E.coli in the E.coli-Salmonella and the E.coli-Shigella mixture occurred from 4 hours incubation following the inoculation. 3. Even the complete inhibition of pathogenic enteric bacterial growth was noticed in the E.coli-Salmonella mixture at overnight incubation. 4. Among the diluted mixtures, 1:100, 1:1,000, and 1:10,000, survival rate of pathogenic enteric bacteria in the mixtures with E.coli showed least affected at the 1:1,000 dilution. 5. It was found that the antagonistic effect aganist groups of Salmonella-shigella was depending upon the groups of the genera.

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The Fate of Tetracycline Resistant Bacteria in Biological Wastewater Treatment Plants (생물학적 하수처리장에서 테트라싸이클린 저항 세균의 거동)

  • Kim, Sungpyo
    • Journal of Korean Society on Water Environment
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    • v.22 no.3
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    • pp.527-533
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    • 2006
  • Tetracycline is one of the mostly used antibiotics around the world. The purpose of this study was to evaluate the fate of two different types of tetracycline resistant bacteria in biological wastewater treatment plants. Tetracycline resistant enterics and heterotrophic bacteria were monitored under two different lab-scale experimental conditions. Tetracycline resistant enteric bacteria showed the lower percentages of total enteric bacteria and net specific growth rate in the monitored activated sludge system as compared to tetracycline resistant heterotrophic bacteria. Therefore, total enterics, potentially E.coli, might not be the best indicator microorganism for evaluating the antibiotic resistant bacteria in biological wastewater treatment plant.

Analysis of Total Bacteria, Enteric Members of γ-proteobacteria and Microbial Communities in Seawater as Indirect Indicators for Quantifying Biofouling

  • Lee, Jin-Wook;Kim, Sung-Min;Jung, Ji-Yeon;Oh, Byung-Soo;Kim, In S.;Hong, Soon-Kang
    • Environmental Engineering Research
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    • v.14 no.1
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    • pp.19-25
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    • 2009
  • In this study, total bacteria, enteric members of the $\gamma$-proteobacteria, and microbial communities in seawater were analyzed as indirect indicators for quantifying biofouling. Biomass in seawater can significantly affect feed water pretreatment and membrane biofouling of reverse osmosis desalination processes. The purpose of this paper is to investigate microbiological quantity and quality of seawater at the potential intake of a desalination plant. For this analysis, the total direct cell count (TDC) using 4'-6-diamidino-2-phenylindole (DAPI)-staining and DNA-based real-time PCR were used to quantify the total bacteria and relative content of enteric members of $\gamma$-proteobacteria in seawater, respectively. In addition, microbial communities were examined using 16S rRNA gene cloning and bacterial isolation to identify the most abundant bacteria for a further biofouling study. The experimental results of this study identified about $10^6$ cells/mL of (total) bacteria, $10^5$ 16S rRNA gene copies/mL of enteric $\gamma$-proteobacteria, and the presence of more than 20 groups of bacteria.

Abtibiotic Resistance in Gram Negative Enteric Bacteria Isolated from Feces of Domestic Animals (가축 분뇨 중의 항생제 내성 균주)

  • 문경호;이종철
    • YAKHAK HOEJI
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    • v.30 no.1
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    • pp.51-54
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    • 1986
  • Fecal samples from Kim-Hae farm animals were examined for the frequency of gram-negative enteric organisms resistant to tetracycline, streptomycin, or penicillin. The propertions of antibiotic resistant to total organisms in fecal specimens of poultry, swine and cow were as follows: 95%, 92%, 70% for tetracycline, 100%, 27%, 9% for streptomycin, 18%, 1%, 1% for penicillin, respectively. The bacteria had multiresistance to antibiotics. These strains had more than one plasmid. From the transformation study, it was concluded that the resistance to streptomycin was attributed to one of these plasmids.

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Development of DNA Chip System for Differential Diagnosis of Porcine Enteric Pathogens

  • Kim, Tae-ju;Cho, Ho-seong;Kim, Yong-hwan;A.W.M. Effendy;Park, Nam-yong
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2003.10a
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    • pp.32-32
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    • 2003
  • Intestinal infections are common in growing pigs and can be caused by multiple pathogens, environmental and management factors [1]. Among the most important viruses in swine enteritis are porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine enteric calicivirus (PECV), porcine group A rotavirus (PRV gp A) and bacteria are Escherichia coli and Salmonella spp. and protozoa is Isospora suis [1]. The DNA chip system can serve as a powerful tool that can be utilized for simultaneous detection of specific pathogenic bacteria strains and viruses [2,3]. The combination of PCR and DNA chip technology will provide a novel method for the detection of porcine enteric pathogens thus revolutionize the diagnosis and management of the disease. The aim of this study is to develop DNA chip system for the rapid and reliable detection of five major porcine enteric pathogens based on oligonucleotide DNA chip hybridization. (omitted)

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Infection Status of Hospitalized Diarrheal Patients with Gastrointestinal Protozoa, Bacteria, and Viruses in the Republic of Korea

  • Cheun, Hyeng-Il;Cho, Shin-Hyeong;Lee, Jin-Hee;Lim, Yi-Young;Jeon, Ji-Hye;Yu, Jae-Ran;Kim, Tong-Soo;Lee, Won-Ja;Cho, Seung-Hak;Lee, Deog-Yong;Park, Mi-Seon;Jeong, Hye-Sook;Chen, Doo-Sung;Ji, Yeong-Mi;Kwon, Mi-Hwa
    • Parasites, Hosts and Diseases
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    • v.48 no.2
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    • pp.113-120
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    • 2010
  • To understand protozoan, viral, and bacterial infections in diarrheal patients, we analyzed positivity and mixedinfection status with 3 protozoans, 4 viruses, and 10 bacteria in hospitalized diarrheal patients during 2004-2006 in the Republic of Korea. A total of 76,652 stool samples were collected from 96 hospitals across the nation. The positivity for protozoa, viruses, and bacteria was 129, 1,759, and 1,797 per 10,000 persons, respectively. Especially, Cryptosporidium parvum was highly mixed-infected with rotavirus among pediatric diarrheal patients (29.5 per 100 C. parvum positive cases), and Entamoeba histolytica was mixed-infected with Clostridium perfringens (10.3 per 100 E. histolytica positive cases) in protozoan-diarrheal patients. Those infected with rotavirus and C. perfringens constituted relatively high proportions among mixed infection cases from January to April. The positivity for rotavirus among viral infection for those aged $\leq$ 5 years was significantly higher, while C. perfringens among bacterial infection was higher for $\geq$ 50 years. The information for association of viral and bacterial infections with enteropathogenic protozoa in diarrheal patients may contribute to improvement of care for diarrhea as well as development of control strategies for diarrheal diseases in Korea.

Imitation of Phosphoenolpyruvate to Oxaloacetate Pathway Regulation of Rumen Bacteria in Enteric Escherichia coli and Effect on C4 Metabolism (반추위 미생물이 가진 Phosphoenolpyruvate에서 Oxaloacetate 경로 조절기작의 대장균에서의 모사와 C4대사의 영향)

  • Kwon Yeong-Deok;Kwon Oh-Hee;Lee Heung-Shick;Kim Pil
    • Microbiology and Biotechnology Letters
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    • v.34 no.1
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    • pp.35-39
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    • 2006
  • One of the fermentative metabolism of enteric Escherichia coli was imitated after rumen bacteria, which have high C4 metabolism. E. coli expresses phosphenolpyruvate carboxylase (PPC) for the pathway between phosphoenolpyruvate (PEP) and oxaloacetate (OAA) during glycolytic condition while expresses phosphoenolpyruvate carboxykinase (PCK) during gluconeogenic condition. In contrast to enteric E. coli, rumen bacteria express the PEP-OAA pathway only by PCK. To verify the effect of the regulation imitation on the C4 metabolism of E. coli, PPC-deficient E. coli strain with PCK expression in glycolytic condition was constructed. The PEP-OAA regulation modified E. coli strain increased 2.5-folds higher C4 metabolite than the wild type strain. The potential use of C4 metabolism by regulation control is discussed.