• Biomolecules & Therapeutics
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• v.21 no.6
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• pp.423-434
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• 2013

The adoption of oligonucleotide aptamer is well on the rise, serving an ever increasing demand for versatility in biomedical field. Through the SELEX (Systematic Evolution of Ligands by EXponential enrichment), aptamer that can bind to specific target with high affinity and specificity can be obtained. Aptamers are single-stranded nucleic acid molecules that can fold into complex three-dimensional structures, forming binding pockets and clefts for the specific recognition and tight binding of any given molecular target. Recently, aptamers have attracted much attention because they not only have all of the advantages of antibodies, but also have unique merits such as thermal stability, ease of synthesis, reversibility, and little immunogenicity. The advent of novel technologies is revolutionizing aptamer applications. Aptamers can be easily modified by various chemical reactions to introduce functional groups and/or nucleotide extensions. They can also be conjugated to therapeutic molecules such as drugs, drug containing carriers, toxins, or photosensitizers. Here, we discuss new SELEX strategies and stabilization methods as well as applications in drug delivery and molecular imaging.

• Molecules and Cells
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• v.24 no.2
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• pp.268-275
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• 2007

Protein phosphorylation plays a key role in signal transduction in cells. Since phosphoproteins are present in low abundance, enrichment methods are required for their purification and analysis. Chemical derivatization strategies have been devised for enriching phosphoproteins and phosphopeptides. In this report, we employed a strategy that replaces the phosphate moieties on serine and threonine residues with a biotin-containing tag via a series of chemical reactions. Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO)-depleted protein extracts prepared from Arabidopsis seedlings were chemically modified for 'biotin-tagging'. The biotinylated (previously phosphorylated) proteins were then selectively isolated by avidin-biotin affinity chromatography, followed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This led to the identification of 31 protein spots, representing 18 different proteins, which are implicated in a variety of cellular processes. Despite its current technical limitations, with further improvements in tools and techniques this strategy may be developed into a useful approach.

• Journal of Korean Academy of Nursing
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• v.49 no.4
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• pp.386-397
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• 2019

Purpose: This study examined the effect of hospitals' family-friendly management on married female nurses' retention intention. The focus was the mediating effects of the work-family interface (work-family conflict, work-family enrichment and work-family balance). Methods: This study was a cross-sectional study. The participants were 307 nurses working at five public and five private hospitals with more than 200 beds in Seoul. Data were collected using structured questionnaires from September 10 to September 17, 2018 and analyzed with SPSS 24.0. Data were analyzed using an independent t-test, a one-way ANOVA, Pearson's correlation coefficients, and multiple regression following the Baron and Kenny method and Sobel test for mediation. Results: There were significant correlations among family-friendly management, the work-family interface, and retention intention. Work-family conflict showed a partial mediating effect on the relationship between family-friendly management and retention intention. Work-family enrichment showed a partial mediating effect on the relationship between family-friendly management and retention intention. Work-family balance showed a partial mediating effect on the relationship between family-friendly management and retention intention. Conclusion: These findings indicate that both hospitals' family-friendly management and nurses' work-family interface are important factors associated with nurses' retention intention. Therefore, hospitals should actively implement family-friendly management for nurses and establish strategies to enhance nurses' work-family interface for effective human resource management.

• Journal of Korean Elementary Science Education
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• v.25 no.3
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• pp.296-306
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• 2006

The purpose of this study is to examine the effectiveness of the summer enrichment programs on Korean gifted students' science learning and creativity development. This program is organized by Purdue University Gilled Education Resource Institute (GERI) in U.S.A. Researchers conducted semi-structured interview with 6 Korean students and observed 12 Korean students and GERI teachers for teacher-student interaction and teaching strategies during science-related classes. From the results, GERI program developed from Purdue 3 stage enrichment model that emphasizes creative teaching strategies, group discussions, and individual research were effective to foster creative thinking of Korean gilled students. Despite their language barriers, Korean gilled students found GERI program experience fun, creative, easy, relaxing, and thereby satisfying for their psychological and academic needs. They expected the level of stimulation in GERI program to be higher and the class to be organized more systematically; however, they reported that the broad range of topics and diverse content of GERI classes helped them develop creativity more than Korean classes. These findings will make contribution to the improvement of the quality of gifted education curriculum and programming in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.1
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• pp.84-92
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• 1999

The rumen microbial ecosystem is coming to be recognized as a rich alternative source of genes for industrially useful enzymes. Recent advances in biotechnology are enabling development of novel strategies for effective delivery and enhancement of these gene products. One particularly promising avenue for industrial application of rumen enzymes is as feed supplements for nonruminant and ruminant animal diets. Increasing competition in the livestock industry has forced producers to cut costs by adopting new technologies aimed at increasing production efficiency. Cellulases, xylanases, ${\beta}$-glucanases, pectinases, and phytases have been shown to increase the efficiency of feedstuff utilization (e.g., degradation of cellulose, xylan and ${\beta}$-glucan) and to decrease pollutants (e.g., phytic acid). These enzymes enhance the availability of feed components to the animal and eliminate some of their naturally occurring antinutritional effects. In the past, the cost and inconvenience of enzyme production and delivery has hampered widespread application of this promising technology. Over the last decade, however, advances in recombinant DNA technology have significantly improved microbial production systems. Novel strategies for delivery and enhancement of genes and gene products from the rumen include expression of seed proteins, oleosin proteins in canola and transgenic animals secreting digestive enzymes from the pancreas. Thus, the biotechnological framework is in place to achieve substantial improvements in animal production through enzyme supplementation. On the other hand, the rumen ecosystem provides ongoing enrichment and natural selection of microbes adapted to specific conditions, and represents a virtually untapped resource of novel products such as enzymes, detoxificants and antibiotics.

• The Journal of Korean Academic Society of Nursing Education
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• v.2
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• pp.20-31
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• 1996

인류가 추구하는 궁극의 가치는 건강한 삶이며 이는 인간의 기본적인 가치이다. 건강관호전달체계에 주어진 사명은 인간의 기본권을 수호하는데 있다. 건강관호전달체계의 주요 구성요원인 간호전문직은 여러차원의 조직(단체)화된 교류를 통해 주어진 사명을 완수하기 위하여 끊임없이 자신들을 강화한다. 간호전문직의 국제적인 교류(단체, 채널)는 다양하나 그 대표적인 조직은 1889년에 회원국 간호협회들에 의하여 설립되어 100주년을 맞는 국제간호협의회 즉 비정부 조직(NGO)과 1848년 세계대전의 종식과 더불어 회원국 정부에 의하여 조직된 세계보건기구 즉 정부조직(GO)이다. 본고는 첫째, 이 두 공인된 국제적인 조직의 정관에 명시된 목적을 재조명하고 이를 통한 간호전문직 강화에 이바지하는 역할을 간략하게 요약한다. 둘째로 1999년도에 창립 100주년을 맞는 국제간호협의회가 20세기를 마무리하고 21세기를 맞기 위한 준비로서 이사와 직원 workshop, 여러차례의 이사회 및 회원국대표자회의 (CNR : Council of National Representatives)를 거쳐 'Towards the 21st Century ; ICN's Strategic Plan 1994-1999'를 확정한 과정을 간략하게 논의한다. 셋째로 'Towards the 21st Century ; ICN's Strategic Plan 1994-1999'의 3개의 Global Objectives와 4개의 National Objectives를 통하여 국제간호협의회가 제시하는 간호전문직의 강화의 방향과 각 목적의 성취를 위한 구체적인 전략을 논의하였다.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2008.04a
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• pp.349-353
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• 2008

With the development of the internet and the spread of 'digital home', the communities in cyber space have been especially revitalized since the information-oriented society. Apartment houses provided networks that are well equipped with internet websites, which build up the houses as a virtual space unit. This study attempted to conducted the questionnaire research for apartment occupants in order to investigate their recognition of and participation in the virtual community and demand for the classified contents The results are as follows. 1) First of all, some easy-access programs are necessary to be groped while aggressive promotional strategies for virtual community that create interests of residents are required to make progress to increase the participation for virtual community 2) 'Gender', 'the form of ownership' and 'level of using the Internet' show significant differences in the case of 'needs in the like-minded people's association', the participation of residents that is induced by both online and offline can cause positive effects on community activity 3) Needs in community enrichment are generally high so that various contents development related to those are necessary. 4) Effects of virtual community on the residential community are on the whole affirmative.

• Journal of Fisheries and Marine Sciences Education
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• v.16 no.1
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• pp.21-34
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• 2004

The purpose of this study was to analyze the current implementation states of the differentiated curriculum at the middle school and to propose ways to improve it. For that purpose this study conducted a survey and had the interviews with the middle school mathematics teachers. The items of survey and interviews consisted of the two main parts: current states and needs about the special supplementary courses and enriched supplementary courses. The results showed that there were discrepancies between the current states and the needs of the differentiated curriculum. This means the efforts to improve the current states are needed. The strategies for improving the implementation of the differentiated curriculum are as follows: 1. The use of various standards including the subject-achievement level and students' or parents' opinions is needed in selecting the students of special supplementary course. 2. More relevant special supplementary materials need to be developed and utilized. 3. The ability grouping is recommended for the differentiated instruction. 4. The deliberation of developing the enriched and supplementary texts is requested. 5. The study for differentiated evaluation of students is demanded.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.22 no.3
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• pp.57-73
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• 2011

The purpose of this study is to suggest the methods for activating blogs in university libraries by investigating and analyzing their current management conditions. For this purpose, first, literatures related to library blogs were reviewed to examine the theoretical background. Second, the contents of university library blogs actively used, were investigated. Third, a survey with librarians in charge of the library blogs was carried out in order to identify problems dealing with blog management. The study's analysis presented the problems that university library blogs faced. And also enrichment strategies for university library blogs are suggested based on the research.

• Journal of The Korean Association For Science Education
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• v.29 no.4
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• pp.359-372
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• 2009

The purpose of this study was to develop the FPHER (problem finding, prediction & discussion, hands-on & experiment, explanation & arrangement, enrichment) instructional model emphasizing the social interactions, and as applied, characteristics of verbal interactions were examined overall and with each step. For this study, this model was applied to the students in 10th grade chemistry classes in a science high school, and their group verbal interactions were recorded and analyzed. The results of this study show that most verbal interactions were classified as on-task interactions in the FPHER instructional model, where suggestions were predominant to the acceptance of opinions. There were a few interactions in the F step, and there were many suggestions relating to the solutions and lacking in confidence in the P step. There were many suggestions relating to the process and orders in the H step. Also, there were many questions, some explanation and dissatisfaction, as well as a lack of confidence in the E step. There were many high-level interactions in the R step, and mainly interactions with worksheets showing high-level problem-solving abilities. More in-depth research is needed to develop the teaching strategies that can activate student-to-student interaction and student-teacher interaction with regard to instructions, enhancing thought as counter-argument, justification or sophistication, based on the instructional model in this study.