• Title/Summary/Keyword: enrichment culture

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Characterization of Biological Treatment by an Isolated Phenol-Degrading Bacterium (페놀분해세균의 분리 및 생물학적 처리 특성)

  • 송형의;김진욱
    • Journal of Environmental Health Sciences
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    • v.24 no.3
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    • pp.54-62
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    • 1998
  • 20 bacterial strains capable of growing on phenol minimal medium were isolated from soil and wastewater by the enrichment culture technique, and among them, one isolate which was the best in the cell growth was selected and identified as Bacillus sp. SH3 by its characteristics. Strain SH3 could grow with phenol as the sole carbon source up to 15 mM, but did not grow in minimal medium containing above 20 mM of phenol. The optimal conditions of temperature and initial pH for growth and phenol degradation were 30$^{\circ}$C and 7.5, respectively. This strain could grow on various aromatic compounds such as catechol, protocatechuic acid, gentisic acid, o-, m-, p-cresol, benzoic acid, p-hydroxybenzoic acid, anthranilic acid, phenyl acetate and pentachlorophenol, and the growth-limiting log P value of strain SH3 on organic solvents was 3.1. In batch culture, strain SH3 degraded 97% of 10 mM phenol in 48 hours. In continuous culture under the conditions of 20 mM of influent phenol concentration and 0.050 hr$^{-1}$ of dilution rate, the treatment rate of phenol was 94%.

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Identification and Optimal Characteristics of Burkholderia sp. SKK381 Degrading Benzene (Benzene 분해 Burkholderia sp. SKK381 분리 및 최적 특성)

  • 강동일;김철경;고창웅;진환준;김장규;김남기
    • KSBB Journal
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    • v.15 no.6
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    • pp.589-593
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    • 2000
  • Several bacterial strains growing on benzene minimal medium were isolated from soil by enrichment culture, Burkholderia sp. SKK381 was identified and selected. In order to determine the ability of Burkholderia sp. SKK381 to degrade benzene. Changes in substrate concentration, cell growth, and pH were monitored from start-up in bath culture. At 30$^{\circ}C$, 1000 ppm of benzene was degraded 100% within 28hours. Cell growth conditions were best at an initial pH of 7.0 and a benzene concentration of 1000 ppm at 30$^{\circ}C$.

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Strategy for enhancing Production of recombinant Protein in tobacco's suspension culture

  • Lee, Dong-Geun;Lee, Jae-Hwa
    • Proceedings of the Korean Society of Life Science Conference
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    • 2002.12a
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    • pp.48-60
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    • 2002
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the production of granulocytes, macrophages, and white blood cells. The effects of osmotic pressure on secretion of human GM-CSF into the culture medium were investigated in suspension cultures of transgenic tobacco cells. An increase in osmotic pressure caused by the addition of mannitol decreased the cell size index, with the effect being more pronounced when cells were measured wet rather than dry. Increased osmotic pressure enhanced the secretion of hGM-CSF. At 90 g/L mannitol, the maximum concentration tested, hGM-CSF was present in the culture medium at 980 ug/L. As the concentration of mannitol increased, the total amount of protein secreted also increased, but was disproportionately enriched in GM-CSF NaCl, another osmoticum, had very similar effects on cell growth and hGM-CSF production, but did not cause enrichment for hGM-CSF Additionally, protein-stabilizing polymer was added to culture broth to enhance stability of secreted recombinant protein. Finally, above two method were applied together to maximize the productivity.

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Environmental toxicity and biodegradation of Pseudomonas sp. EL-G527 producing biosurfactant

  • Mi Seon, Cha;Hong Ju, Son;Sang Jun, Lee
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2002.05b
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    • pp.452-454
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    • 2002
  • A biosurfactant-producing microorganism, .Pseudomouos sp. EL-G527 was isolated from activated sludge by enrichment culture when grown on mineral salt medium containing n-hexadecane as a carbon source. The biosurfactant from .Pseudomonar sp. EL-G527 exhibited lesser toxicity to bacterial population than synthetic surfactants and in the biodegradation test, biosurfactant was rapidly degraded and lost its activity as surface active material after 1 day incubation. In this study, the biosurfactant from Pseudomonas sp. EL-G527 was effective surface-active compound, more biodegradable and less toxic to microbial ecosystem than various synthetic surfactants.

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Antifungal Compound Produced by Bacillus sp. TBM912 (Bacillus sp. TBM912가 생산하는 항균물질)

  • 주우홍;한수지;최용락;정영기
    • Journal of Life Science
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    • v.14 no.1
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    • pp.193-197
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    • 2004
  • A continuous enrichment culture procedure was used to isolate bacteria from various soil sources capable of suppressing large patch disease of turfgrass. Six isolates consistently suppressed large patch in turfgrass, and ranged in the spectrum of extracellular enzymes that they expressed. The best disease- suppressing isolate, TBM912, expressed protease, CMCase, and pectinase activity and inhibited the growth of Rhizectonin solani and Betrytis cinerea in vitro. Here we show that this strain also produces an antibiotic that was identified by TLC, SDS-PACE and HPLC analysis as lipopeptide.

Growth, survival and pigmentation of turbot(Scophthalmus maximus) larvae fed live-prey enrichment

  • Liang Mengqing;Chang Qing;Wang Jialin;Park, Byeong-Dae;Kim, Kwang-Yang
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.199-200
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    • 2003
  • After more than 5 years of turbot culture in China, low percent survival and high occurrences of abnormally pigmented juveniles are still major problems for fish farmer. Much research has been directed toward determining the optimal feeding strategies and nutritional requirements for marine flatfish larvae, and considerable advances have been made. The most common live feeds include :Artemia, rotifer and copepods. (omitted)

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Carriage of Salmonella in Pigs Slaughtered at Daegu Slaughter-house (대구시(大邱市) 도축장(屠畜場)에서 처리(處理)된 돼지의 Salmonella 속균(屬菌)의 보균상태(保菌狀態))

  • Tak, Ryunbin
    • Korean Journal of Veterinary Research
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    • v.18 no.1
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    • pp.15-18
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    • 1978
  • Ten strains of Salmonella were isolated from feces and lymph nodes of swine slaughtered at Daegu slaughter-house and the rate of isolation was 6.0 percent. Nine strains of Salmonella were isolated by enrichment in selenite F broth and one strain by direct culture on SS agar, but none of Salmonella were isolated from MacConkey ager and in SS broth. Among Salmonella isolated, Salmonella typhimurium occupied over half (6 strains) and the importance of Salmonella in swine for the incidence of food poisoning in man was discussed.

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Effects of $CO_2$ Enrichment on the Differentiation and Growth in tissue culture of Panax ginseng C. A. Meyer (人參(인삼) 조직배양(組織培養)에 있어 $CO_2$ 처리(處理)가 식물체(植物體) 분화(分化) 및 생장(生長)에 미치는 영향(影響))

  • Chung, Chan-Moon;Bae, Kil-Kwan;Aoki, Masatoshi
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.1
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    • pp.14-20
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    • 2000
  • This experiment was conducted to investigate the effects of length of storage period under low temperature, $CO_2$ enrichment and addition of plant growth regulators in Murashige and Skoog medium on the plant regeneration of Korean ginseng (Panax ginseng C. A. Meyer). Seeds were treated for 60 and 80 days respectively under $5^{\circ}C$ environment. 2500ppm of $CO_2$ was enriched by ventilation. Three plant growth regulators added to the medium were Indolbutyric acid, Benzyladenin and Gibberellic acid (GA3). The result indicated that : The capacity of differentiation was higher in the aged cotyledons from the seeds treated for 80 days under low temperature condition than in those treated for 60 days. $CO_2$ enrichment had stimulating effects on the growth and development of shoot primordium significantly but less effects on the formation of adventitious buds. From one zygotic embryo hundreds of plantlets were differentiated. $CO_2$ enrichment had effects on the formation of both indirect somatic embryo and direct somatic embryo. Indirect somatic embryo showed little growth and differentiation, being undifferentiated vascular stele and epicotyl. Direct somatic embryos were formed on the epidermis of backside basal part of cotyledon. Those embryos developed to whole plant having latent bud.

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Microbial Communities of the Microbial Fuel Cell Using Swine Wastewater in the Enrichment Step with the Lapse of Time (가축분뇨를 이용한 미생물연료전지의 농화배양 단계에서 미생물 군집 변화)

  • Jang, Jae Kyung;Hong, Sun Hwa;Ryou, Youg Sun;Lee, Eun Young;Chang, In Seop;Kang, Young Koo;Kim, Jong Goo
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.12
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    • pp.973-977
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    • 2013
  • These studies were attempted to investigate the change of microbial community of anode of microbial fuel cell using swine wastewater in the enrichment step with the lapse of time. Microbial fuel cells enriched by a 1 : 1 mixture of anaerobic digestive juices of the sewage treatment plant and livestock wastewater. Enrichment culture step was divided into three stages to indentify the microorganisms. It was separated by each lag phase, exponential phase, and stationary phase. These steps were determined by the change of the current value. The current after enrichment was generated about $0.84{\pm}0.06mA$. We were cut out the different 17 bands in the DGGE fingerprint gel to do sequencing. The bands which the concentration was increasing or newly appearing with the lapse of time were included for this study. In the lag and exponential phase, Clostridium, Rhodocyclaceae, Bacteriodetes, and Uncultured bacterium etc. were detected. There were in the stationary phase Geobacter sp., Rhodocyclaceae, Candidatus, Nitrospira, Flavobactriaceae and uncultured bacterium etc. Geobactor among microorganisms detected in this study is known as the Electrochemically active microorganisms. It may include electrochemically active microorganisms to be considered as electrical activity microorganisms.

Bacteriological Study of Listeria sp. Isolated from Seawater and Sea Food (해수와 해산물로부터 Listeria 속의 분리와 세균학적 조사)

  • 강치희;이만효;황용일
    • Journal of Life Science
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    • v.13 no.4
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    • pp.390-399
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    • 2003
  • Four species of the genus of Listeria were isolated from seawater and sea food in Kyungnam province, South Korea. These isolated strains were classified into Listeria sp. from different samples by appropriate cultivation conditions and biochemical tests including serological test. In a day enrichment cultivation, the following strains were found out of 100 samples: L. innocua (35%), L. ivanovii (4%), L. monocytogenes (4%), and L. welshimeri (1%). For seven days enrichment culture, L. innocua (38%), L. ivanoii (5%), L. monocytogenes (7%), and L. welshimeri (1%) were isolated. From these results, Listeria species were more efficiently isolated in seven day enrichment broth than in one day enrichment. However, these isolated Listeria species were less grown in the selective medium than in the enrichment medium. Isolation rates of Listeria species showed differency for each sample and Listeria species were more abundantly isolated in shrimps (80%) and crayfishes (80%) than little neck clams (50%), seawater (25%) and mussels (20%). From the results of serological classes for the seven L. monocytogenes, two strains were defined as type I and the other five strains as type IV.