• 제목/요약/키워드: enrichment

검색결과 1,855건 처리시간 0.028초

Degradation of Anthracene by a Pseudomonas strain, NGK1

  • Shinde Manohar;Kim, Chi-Kyung;Tim
    • Journal of Microbiology
    • /
    • 제37권2호
    • /
    • pp.73-79
    • /
    • 1999
  • Pseudomonas sp. NGK1, isolated by naphthalene enrichment culture technique, is capable of degrading anthracene as a sole source of carbon and energy. The organism degraded anthracene through the intermediate formation of 1,2-dihydroxyanthracene, 2-hydroxy-3-naphthoic acid, salicylate, and catechol. The intermediates were isolated and characterized by TLC, spectrophotometry, and HPLC analysis. The cell free extract of anthracene-grown cells showed activities of anthracene dioxygenase, 2-hydroxy-3-naphthylaldehyde dehydrogenae, 2-hydroxy-3-naphthoate hydroxylase, salicylate hydroxylase and catechol 2,3-dioxygenase. The formed catechol as a metabolite is degraded through meta-cleavage with the formation of ${\alpha}$-hydroxymuconic semi-aldehyde.

  • PDF

Optimal Resolution of L-Carnitine from Racemic DL-Carnitine by Enterobacter sp. Assimilating D-Carnitine

  • Hwang, Ki-Chul;Bang, Won-Gi
    • Journal of Microbiology and Biotechnology
    • /
    • 제7권5호
    • /
    • pp.318-322
    • /
    • 1997
  • In order to isolate a microorganism having preferential degradation of D-carnitine from DL-carnitine, a bacterium assimilating D-carnitine as a sole carbon and energy source was isolated from soil by enrichment culture and partially identified as Enterobacter sp. Also, a mutant having lessened L-carnitine decomposition rates was selected with nitrosoguanidine mutagenesis, which led to decrease the specific activities of carnitine dehydrogenase (7.6-fold) and ${\beta}$-hydroxybutyrate dehydrogenase (9.5-fold) as compared to the wild strain. Meanwhile, optimal culture conditions for optical resolution of DL-carnitine were investigated. Under optimal conditions, 3.53 g/l L-carnitine was obtained from 20 g/l DL-carnitine, which corresponded to 35.3% L-carnitine yield and 97.9% optical purity.

  • PDF

Characterization of a New Acidophilic Acetobacter sp. Strain HA Isolated from Korean Traditional Fermented Vinegar

  • CHUN, HONG-SUNG;SUNG-JUN KIM
    • Journal of Microbiology and Biotechnology
    • /
    • 제3권2호
    • /
    • pp.108-114
    • /
    • 1993
  • A new strain of acidophilic, acetogenic bacterium, Acetobacter sp. strain HA was isolated by selective enrichment from the traditionally fermented rice wine vinegar in Korea. It was a gram-negative, non-motile short rod and oxidized acetate and lactate. The optimal temperature and pH for growth were $28^{\circ}C$ and 4.0, respectively. The strain HA differed from other Acetobacter species by growing well on methanol, xylitol, inositol, dulcitol, D-xylose, L-arabinose, and D-mannose as sole sources of carbon and energy. The isolated strain HA did not produce $\gamma$-pyrones from glucose and did not produce ketone bodies from glycerol. The quinone system used in this study was an ubiquinone-9 isoprene unit. The guanine-plus-cytosine content of the DNA was 50.7 mol%, and the major cellular fatty acids were $C_{18:1} and C_{16:0}$.

  • PDF

수포작용제(HD) 분석을 위한 단일방울 미세농축법 연구 (Single Drop Microextraction for Analysis of Mustard Gas(HD))

  • 박양기;김성기;손병훈;박명주
    • 한국군사과학기술학회지
    • /
    • 제11권2호
    • /
    • pp.95-100
    • /
    • 2008
  • A fast, simple, inexpensive, and reproducible sample preparation for extraction and analysis of HD in water was studied using single-drop microextraction(SDME) and GC-MS. Operation parameters, such as extraction solvent, extraction time were optimized. The optimized conditions were $1\{mu}L$ trichloroethylene and 10 min extraction time. In these conditions, about 42 times higher enrichment factor(EF) was obtained. The detection limit of HD was $1\{mu}g/L$, and the precision expressed as relative standard deviation was about 9.0%.

비대칭 Polysulfone계(PS/PES/PPS)막 제조시 물리적 인자의 영향 및 기체투과 특성 (Physical Properties and Permeation Characteristics of Polysulfone group(PS/PES/PPS) membrane for Gas Permeation)

  • 박영해
    • 한국산업융합학회 논문집
    • /
    • 제4권1호
    • /
    • pp.77-85
    • /
    • 2001
  • This paper was to investigate the preparation of polysulfone(PS), polyethersulfone(PES) and polyphenylsulfone(PPS) membrane. The thermal property of PPS was higher than that of others. From the result of SEM, the concentration of polymer was found to have a significant effect on the structure of membrane, and the structure of membrane made of PES is found to have regular micell form of asymmetry. Permeability and selectivity for oxygen and nitrogen gas in the air were analyzed by GC. Permeabilities of the membrane made of PES for oxygen and nitrogen in air, 1.5 and $0.7(x10^9[cm^3(STP)cm/cm^2seccmHg]) $, respectively was higher than that of others. and Selectivity of the membrane made of PPS for oxygen to nitrogen gas in air was 2.9.

  • PDF

Effects of Employees' Job Characteristics, Commitment, and Self-Leadership on Organizational Citizenship Behavior

  • Eom, Keun;Yang, Hoe-Chang
    • 유통과학연구
    • /
    • 제12권7호
    • /
    • pp.13-21
    • /
    • 2014
  • Purpose - This study reveals antecedent factors to help develop expressway rest areas in which employees were exposed to inferior environments, using Hackman and Oldham's Job Characteristics Theory. Research design, data, and methodology - The survey was conducted on 148 expressway rest area employees in Gyeonggi-do. After excluding questionnaires with invalid responses, 141 questionnaires were analyzed. Results - The sub-factors of job characteristics had a positive influence on the organizational commitment of employees, and organizational commitment had a positive influence on organizational citizenship behavior (OCB). Further, the results of mediation effects showed that organizational commitment and self-leadership were partially mediated. Finally, the results of the moderation effect of self-leadership showed that self-leadership was thought to be vital to let members endeavor to promote the competitiveness of expressway rest areas. Conclusions - The expressway rest area company needed to design jobs to let employees be self-motivated, productive, positive, responsible, and aspirational; job redesign, including job enrichment and job enlargement can be considerably helpful for affirmative influence.

균열 유한 요소의 개발 (Development of finite 'crack' element)

  • 조영삼;전석기;임세영
    • 한국전산구조공학회:학술대회논문집
    • /
    • 한국전산구조공학회 2004년도 봄 학술발표회 논문집
    • /
    • pp.381-388
    • /
    • 2004
  • We propose a 2D 'crack' element for the simulation of propagating crack with minimal remeshing. A regular finite element containing the crack tip is replaced with this novel crack element, while the elements which the crack has passed are split into two transition elements. Singular elements can easily be implemented into this crack element to represent the crack-tip singularity without enrichment. Both crack element and transition element proposed in our formulation are mapped from corresponding master elements which are commonly built using the moving least-square (MLS) approximation only in the natural coordinate. In numerical examples, the accuracy of stress intensity factor K/sub I/ is demonstrated and the crack propagation in a plate is simulated.

  • PDF

Structure and chemical composition of $CsB_{3}O_{5}$ (CBO) optical surface

  • V.V. Atuchin;V.G. Kesler;L.D. Pokrovsky;N. Yu. Maklakova;M. Yoshimura;N. Ushiyama;T. Matsui;K. Kamimura;Y. Mori;T. Sasaki
    • 한국결정성장학회지
    • /
    • 제13권1호
    • /
    • pp.19-23
    • /
    • 2003
  • Polished surface of $CsB_{3}O_{5}$ (CBO) has been observed by reflection high energy electron diffraction (RHEED) and X-ray photoelectron spectroscopy (XPS). For comparison, electronic properties of CBO powder have been studied by XPS. It has been found that the crystal surface is covered by thick amorphous layer with chemical composition closely related to that of CBO. Great enrichment of top surface by cesium, ~30 % in reference to the bulk of the modified layer, has been displayed by depth profiling.

A Study on Privacy Issues and Solutions of Public Data in Education

  • Jun, Woochun
    • International Journal of Internet, Broadcasting and Communication
    • /
    • 제12권1호
    • /
    • pp.137-143
    • /
    • 2020
  • With the development of information and communication technology, various data have appeared and are being distributed. The use of various data has contributed to the enrichment and convenience of our lives. Data in the public areas is also growing in volume and being actively used. Public data in the field of education are also used in various ways. As the distribution and use of public data has increased, advantages and disadvantages have started to emerge. Among the various disadvantages, the privacy problem is a representative one. In this study, we deal with the privacy issues of public data in education. First, we introduce the privacy issues of public data in the education field and suggest various solutions. The various solutions include the expansion of privacy education opportunities, the need for a new privacy protection model, the provision of a training opportunity for privacy protection for teachers and administrators, and the development of a real-time privacy infringement diagnosis tool.

한국표준형 원전 증기발생기 전열관 축방향 ODSCC 발생원인 분석 (Root Cause Analysis of Axial ODSCC of Steam Generators Tubes of OPR1000)

  • 김홍덕;박수기;임창재;정한섭
    • 한국압력기기공학회 논문집
    • /
    • 제6권1호
    • /
    • pp.83-88
    • /
    • 2010
  • Domestic nuclear steam generators with Alloy 600 HTMA tubes have experienced axial cracking at eggcrate tube support plates(TSPs). The axial stress corrosion cracks were observed at the crevice between outside of tubes and eggcrate TSPs. The root cause of axial cracking was investigated by thermal hydraulic analysis and sludge distribution diagnosis. It is suggested that deposition of sludge at eggcrate TSPs could increase the outside surface temperature of tube and promote the enrichment of impurities at crevice, and thus accelerate cracking. Additionally strategy for reducing the sludge ingress to steam generators is discussed.

  • PDF