• Bulletin of the Korean Chemical Society
• /
• 제8권3호
• /
• pp.133-137
• /
• 1987

Polysiloxane SE-54 liquid phase was immobilized on the support surface as coated in thin film via in-situ cross-linking. The cross-linking between liquid molecules was initiated by dicumylperoxide. Among the supports investigated, only Chromosorb W provided the cross-linkable surface. The optimal in-situ cross-linking was achieved when Chromosorb W was coated with 5% (w/w) SE-54 and cross-linked with 1% (w/w) dicumylperoxide. The cross-linked support was useful for the trace analysis as well as for the trace enrichment.

• Bulletin of the Korean Chemical Society
• /
• 제22권5호
• /
• pp.503-506
• /
• 2001

Separation of lithium isotopes was investigated by chemical ion exchange with a hydrous manganese(IV) oxide ion exchanger using an elution chromatography. The capacity of manganese(IV) oxide ion exchanger was 0.5 meq/g. One molar CH3COO Na solution was used as an eluent. The heavier isotope of lithium was enriched in the solution phase, while the lighter isotope was enriched in the ion exchanger phase. The separation factor was calculated according to the method of Glueckauf from the elution curve and isotopic assays. The single stage separation factor of lithium isotope pair fractionation was 1.021.

• 방사성폐기물학회지
• /
• 제20권1호
• /
• pp.111-118
• /
• 2022

The denuclearization of North Korea was unpredictable and resulted in radical changes. Despite the skepticism and disappointment surrounding denuclearization, it is important for certain verification technologies to establish what is technically possible or practically impossible, and how reliable these technical means are. This article presents the technical hurdles in nuclear verification by systematically categorizing them into issues of correctness and completeness. Moreover, it addresses the safety and security risks during the denuclearization process, including the radiological impact on humans, environmental effects, and the illegal transfer of material, information, and technologies.

• Molecules and Cells
• /
• 제46권1호
• /
• pp.65-67
• /
• 2023

SMILES (simplified molecular-input line-entry system) information of small molecules parsed by one-hot array is passed to a convolutional neural network called black box. Outputs data representing a gene signature is then matched to the genetic signature of a disease to predict the appropriate small molecule. Efficacy of the predicted small molecules is examined by in vivo animal models. GSEA, gene set enrichment analysis.

• 한국수소및신에너지학회논문집
• /
• 제25권5호
• /
• pp.533-540
• /
• 2014

The remanufacturing industry for automotive parts is a major issue which affects the environment protection and CO2 reduction throughout the world. Beside this, remanufacturing technologies of worn-out diesel engines have been developing to make as close to new as possible. In this study, the characteristics of the engine-power output and exhaust emissions of remanufactured diesel engine by hydrogen enrichment are evaluated by measuring the engine and vehicle test. Moreover, with worn-out diesel engine and first generation common-rail engine, we compared by testing their characteristics, resulting in the restoration of engine-power output more than 93%, as well as marvelously reduces the THC and NOx emission. At a guess, high pressure injection of diesel increases fuel atomization characteristics with excellence combustion efficiency, resulting in reduction of THC emission. Also, rapid cooling of EGR decreases combustion temperature, resulting in reduction of NOx emission. Consequently, these remanufacturing for diesel engine enables worn-out diesel engine to have restoration to the original state. Simultaneously achieved 2 goals called that CO2 emission reduction and protection of environment by remanufacturing engine.

• Molecules and Cells
• /
• 제24권3호
• /
• pp.329-337
• /
• 2007

An enrichment semi-nested PCR procedure was developed for detection of Clostridium botulinum types A, B, E, and F. It was applied to sediment samples to examine the prevalence of C. botulinum in the Korean environment. The first pair of primers for the semi-nested PCR was designed using a region shared by the types A, B, E, and F neurotoxin gene sequences, and the second round employed four nested primers complementary to the BoNT/A, /B, /E, and /F encoding genes for simultaneous detection of the four serotypes. Positive results were obtained from the PCR analysis of five of 44 sediments (11%) collected from Yeong-am Lake in Korea; all were identified as deriving from type B neurotoxin (bontb) genes. Two of the C. botulinum type B organisms were isolated, and their bontb genes sequenced. The deduced amino acid sequences of BoNT/B showed 99.5 and 99.8% identity with the amino acid sequence of accession no. AB084152. Our data suggest that semi-nested PCR is a useful tool for detecting C. botulinum in sediments, and renders it practicable to conduct environmental surveys.

• 한국콘텐츠학회논문지
• /
• 제12권8호
• /
• pp.388-398
• /
• 2012

본 연구에서는 도시 하수관거시스템의 침수위험순위 평가를 위하여 엔트로피(Entropy) 기법 및 다기준 의사결정기법의 하나인 프로메티(Preference Ranking Organization METHod for Enrichment Evaluations, PROMETHEE)를 적용하였다. 엔트로피와 프로메티를 이용한 침수위험순위 산정에 있어서 평가 대상이 되는 항목들은 두 가지의 접근방식으로 선정되었다. 먼저 침수발생 및 피해 규모에 영향을 미치는 인자로 지형 환경적 요인으로써 유역의 평균고도, 평균경사, 유역폭, 인구수 및 관밀도를 선정하였다. 또한 각 시스템별 침수가 가장 크게 발생하는 초과강우사상을 선정 및 적용하여 시스템내 월류발생량 및 발생지점 두 가지를 이용한 치수안전성 지수를 추가적으로 고려하였다. 본 논문에서 적용된 엔트로피기법과 프로메티에 의한 도시 하수관거시스템 침수위험순위 평가는 추후 하수관거 정비사업의 계획 및 추진에 있어서 각 시스템별 위험도에 따른 개량우선순위 결정 및 평가지표로서 활용될 수 있을 것이다.

• 자원환경지질
• /
• 제30권6호
• /
• pp.587-601
• /
• 1997

This study had three purposes: (1) to investigate the enrichment levels and dispersion patterns of potentially toxic elements in the rock-soil-plant system; (2) to evaluate the uptake ratios of heavy metals from soils into plants and (3) to assess the chemical speciation of heavy metals in soils. Rock, surface soil and plant samples were collected in the Boeun area underlain by black shales of the Okchon Zone. These samples were analyzed for multi-elements using INAA, ICP-AES and AAS. The maximum abundance of U in black shales is 16 mg/kg and radioactivity counts up to 300 cpm. In particular, Mo, V, Ba, Cd, Pb and U are enriched in black shales. Most of soils derived from black shales show high concentrations of U, As, Mo, Ba, Cu, Cd, Pb, Zn and mean concentrations of As and Mo in soils (20 mg/kg of As and 6.6 mg/kg of Mo) are higher than the permissible level suggested by Kloke (1979). Enrichment index values of soils are calculated and higher than 1.0 in the black shale area with the highest value of 6.4. Mean concentration of Cd in plants is higher than those of Cu, Pb and Zn. The concentration of Cd in plant species decreases in the order of Chinese cabbage > red pepper > soybean=sesame > rice stalk > com > rice grain. The biological absorption coefficients (BAC) in plants are in the order of Cd > Zn=Cu > Pb, which suggests that Cd is more bioavailable to plants than Cu, Pb and Zn. From the results of sequential extraction analysis of soils, relatively high proportion of Cu, Pb and Zn are present as residual fractions whereas that of Cd as non-residual fractions. Cadmuim occurs predominantly as exchangeable/water-acid soluble phase in soils, and Cd is more mobile and bioavailable than Cu, Pb and Zn.

• 대한수의학회지
• /
• 제38권4호
• /
• pp.763-770
• /
• 1998

Salmonella typhimurium is a causitive agent of diarrhea, fever, gastroenteritis, septicemia and sudden death in piglet. The currently used methods such as IFA, ELISA, DNA hybridization assay is needed a long-time and difficult to detect the organism in carrier animal or contaminated sample with other agents. However, it is important to detect rapidly and sensitively S typhimurium in piglet with other infectious pathogens to minimize an economic loss. Two sets of PCR primer, rfbJ forward primer(5'-AGAATATGTAATTGTCAG-3') and reverse primer(5'-TAACCGTTTCAGTAGTTC-3') were designed to amplify a 882 by fragment of Salmonella serovar type B gene. The target genomic DNA for PCR was extracted from the cultivated materials with various enrichment periods in a nonselective enrichment agar and broth with clinical specimens. The PCR is carried out here made it possible to detect the gene from two hours. Also, the amplified fragment with PCR was cloned into pGEM-T vector and digested with restrict enzyme, and sequenced for the identification of Salmonella serotype B rfbJ gene. Duplicated cultivation agar-broth followed by PCR were performed to develop a rapid and sensitive detection of S typhlmurium based on serovar type. This duplicated cultivation-PCR method provides a sensitive and rapid diagnostic tool to detect Salmonella from infected piglet with improved sensitivity.

• Journal of Microbiology and Biotechnology
• /
• 제21권6호
• /
• pp.590-598
• /
• 2011

Bacterial assimilation of $CO_2$ into stable biomolecules using electrochemical reducing power may be an effective method to reduce atmospheric $CO_2$ without fossil fuel combustion. For the enrichment of the $CO_2$-fixing bacteria using electrochemical reducing power as an energy source, a cylinder-type electrochemical bioreactor with a built-in anode compartment was developed. A graphite felt cathode modified with neutral red (NR-graphite cathode) was used as a solid electron mediator to induce bacterial cells to fix $CO_2$ using electrochemical reducing power. Bacterial $CO_2$ consumption was calculated based on the variation in the ratio of $CO_2$ to $N_2$ in the gas reservoir. $CO_2$ consumed by the bacteria grown in the electrochemical bioreactor (2,000 ml) reached a maximum of approximately 1,500 ml per week. Time-coursed variations in the bacterial community grown with the electrochemical reducing power and $CO_2$ in the mineral-based medium were analyzed via temperature gradient gel electrophoresis (TGGE) of the 16S rDNA variable region. Some of the bacterial community constituents noted at the initial time disappeared completely, but some of them observed as DNA signs at the initial time were clearly enriched in the electrochemical bioreactor during 24 weeks of incubation. Finally, Alcaligenes sp. and Achromobacter sp., which are capable of autotrophically fixing $CO_2$, were enriched to major constituents of the bacterial community in the electrochemical bioreactor.