• 제목/요약/키워드: enriched food

검색결과 346건 처리시간 0.026초

The effect of boiled feed on trace elements of longissimus dorsi muscle in Hanwoo steers

  • Kim, Jaeyoung;Jung, Meyungok;Jin, Sangkeun;Seo, Hyunseok;Ha, Jungheun;Choi, Jungseok
    • Journal of Animal Science and Technology
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    • 제63권1호
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    • pp.160-169
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    • 2021
  • Boiled feed is obtained by mixing and boiling agricultural by-products such as rice straw, rice bran, and bean curd with grains. The study explored the change in fatty acid, free amino acid, nucleotide, mineral, cholesterol, myoglobin and collagen of longissimus dorsi muscle in Hanwoo steers fed with boiled feed. Forty steers, 20 heads per group, were divided into two groups: a control group and a boiled feed group. The steers were raised for 10 months. The boiled feed group was enriched with palmitoleic acid, oleic acid, arachidonic acid and unsaturated fatty acids compared with the control group. There were no significant differences in amino acid and nucleic acid composition between the two groups. The boiled feed group contained higher levels of iron and manganese in the boiled feed group compared with the control group. The total cholesterol level was significantly increased, whereas calorie levels, myoglobin and collagen composition showed no differences. As the supply of boiled feed increases the content of fatty acids, unsaturated fatty acids and minerals related to flavor, it should be a feed that leads to the production of high-quality beef.

Antibacterial Activity of Clove Oil against Foodborne Pathogenic Bacteria and Sensory Attributes in Clove Oil-Enriched Dairy Products: A Preliminary Study

  • Chon, Jung-Whan;Seo, Kun-Ho;Bae, Dongryeoul;Kim, Binn;Jeong, Dongkwang;Song, Kwang-Young
    • Journal of Dairy Science and Biotechnology
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    • 제38권4호
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    • pp.197-206
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    • 2020
  • This study was conducted to evaluate the antibacterial activity against Cronobacter sakazakii and Salmonella enteritidis as well as the sensory attributes of milk products supplemented with various concentrations (control, 0.5%, 1.0%, 1.5%, and 2.0%) of clove oil. In this study, clove oil was shown to have strong antibacterial activities. In addition, all the samples were assessed by ten researchers trained in five sensory attributes, namely, taste, flavor, color, texture, and overall acceptability. Compared to the control, 5% clove oil supplemented was the best in market milk, while in yogurt and kefir, 1.0% supplementation was the best. In terms of sensory attributes, the low score of color and flavor of market milk, yogurt, and kefir is attributed to the characteristics of the supplemented clove oil. Consequently, this study presents the possibility of producing bio-functional milk products supplemented with clove oil, and for controlling the growth of foodborne pathogenic bacteria in milk products using clove oil.

Metabolites of Kimchi Lactic Acid Bacteria, Indole-3-Lactic Acid, Phenyllactic Acid, and Leucic Acid, Inhibit Obesity-Related Inflammation in Human Mesenchymal Stem Cells

  • Moeun Lee;Daun Kim;Ji Yoon Chang
    • Journal of Microbiology and Biotechnology
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    • 제34권2호
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    • pp.306-313
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    • 2024
  • Given the diversity of vegetables utilized in food fermentation and various lactic acid bacteria (LAB) populations in these materials, comprehensive studies on LAB from vegetable foods, including kimchi, are imperative. Therefore, this study aimed to investigate the obesity-related inflammation response of three metabolites-phenyllactic acid (PLA), indole-3-lactic acid (ILA), and leucic acid (LA)-produced by LAB (Companilactobacillus allii WiKim39 and Lactococcus lactis WiKim0124) isolated from kimchi. Their effects on tumor necrosis factor-α-induced changes in adipokines and inflammatory response in adipose-derived human mesenchymal stem cells were examined. The study results showed that PLA, ILA, and LA, particularly PLA, effectively reduced lipid accumulation and triglyceride, glycerol, free fatty acid, and adiponectin levels. Furthermore, the identified metabolites were found to modulate the expression of signaling proteins involved in adipogenesis and inflammation. Specifically, these metabolites were associated with enriched expression in the chemokine signaling pathway and cytokine-cytokine receptor interaction, which are critical pathways involved in regulating immune responses and inflammation. PLA, ILA, and LA also suppressed the secretion of pro-inflammatory cytokines and several inflammatory markers, with the PLA-treated group exhibiting the lowest levels. These results suggest that PLA, ILA, and LA are potential therapeutic agents for treating obesity and inflammation by regulating adipokine secretion and suppressing pro-inflammatory cytokine production.

Anti-Inflammatory Activity of the Total Flavonoid Fraction from Broussonetia papyrifera in Combination with Lonicera japonica

  • Jin, Jeong-Ho;Lim, Hyun;Kwon, Soon-Youl;Son, Kun-Ho;Kim, Hyun-Pyo
    • Biomolecules & Therapeutics
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    • 제18권2호
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    • pp.197-204
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    • 2010
  • To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction. EBP and the ethanol extract of the whole Lonicera japonica (ELJ) plant were then mixed at a ratio of 1:1 (w/w) to give a new preparation (BL) in the hope of obtaining an optimal formula with a higher anti-inflammatory activity. Evaluation of the effects of these preparations on A23187-treated rat basophilic leukemia (RBL-1) cells revealed that EBP potently inhibited 5-lipoxygenase (5-LOX), while ELJ showed weak inhibition. Additionally, the mixture (BL) clearly showed stronger inhibitory effects against 5-LOX than either preparation alone. These preparations also inhibited cyclooxygenase-2-catalyzed $PGE_2$ and inducible nitric oxide (NO) synthase-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells. When tested against arachidonic acid-induced mouse ear edema, EBP showed strong inhibitory activity at doses of 5-200 mg/kg when administered orally, but BL had obviously stronger inhibitory effects. When tested against ${\lambda}$-carrageenan-induced paw edema in mice, BL showed a potent and synergistic anti-inflammatory effect. In addition, in the acetic acid-induced writhing test, BL was found to have strong analgesic activity at 50-400 mg/kg. Taken together, these results indicate that each of these preparations exert anti-inflammatory activity in vitro and in vivo. In particular, BL showed stronger anti-inflammatory activity than EBP, and these anti-inflammatory effects were partially related to the inhibition of eicosanoid and NO production. BL may be useful for the treatment of human inflammatory disorders.

퇴비에서 식중독균 검출을 위한 DNA 추출 방법 비교 (Comparison of DNA Extraction Methods for the Detection of Foodborne Pathogenic Bacteria from Livestock Manure Composts)

  • 김성연;서동연;문지영
    • 한국식품위생안전성학회지
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    • 제34권6호
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    • pp.557-561
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    • 2019
  • 본 연구에서는 가축분퇴비에 존재할 수 있는 식중독균의 검출을 위하여 기존의 배양을 이용한 방법을 대체할 수 있는 real-time PCR을 적용하고자 하였으며, 이에 따라 유전자 증폭에 영향을 미치는 DNA 추출 방법에 따른 식중독균 검출 효율을 비교하였다. 적용한 방법은 가열 처리, 유기용매 및 흡착제 처리, 효소 처리의 3가지로 구분할 수 있으며, 각 방법에 따른 DNA의 검출 효율을 실험 결과로 나타내었다. 가열 처리 방법에서는 가열 시간의 증가에 따라 DNA 검출 효율이 높아지는 경향을 나타냈으며, 유기용매 및 흡착제는 효과를 나타내지 않았고, 효소 처리의 경우에는 그람 양성균 보다는 그람 음성균의 DNA가 추출 효율이 더 높은 것으로 나타났다. 결론적으로 퇴비에서 30분 이상의 가열 처리와 효소의 처리를 통한 DNA 추출 방법은 real-time PCR을 적용한 식중독균 검출에 적합한 것으로 판단된다.

우유 첨가가 두부 품질에 미치는 영향 (Effects of Cow에s Milk Addition on the Quality of Soybean Curd)

  • 김중만;김형태;최용배;황호선;김태영
    • 한국식품영양과학회지
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    • 제22권4호
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    • pp.437-442
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    • 1993
  • 두유에 우유를 각각 10, 20, 30, 40, 50%(v/v) 첨가하고 두부를 제조하여 일반성분의 함량변화, 수율과 pH 변화, 관능검사, 견고성, 무기이온 함량의 변화 및 amino acid 조성을 조사 비교하였다. 수분함량은 우유첨가로 다소 감소하는 경향을, 당, 지방, 단백질, 회분의 함량은 우유의 첨가량이 증가함에 따라 증가하였고 pH 값은 우유의 첨가량이 증가함에 따라서 높아졌다. 두부의 무게와 용적은 우유의 첨가량에 비례적으로 증가하였다. 굳기는 우유 첨가량에 비례하여 증가하였으며 금속이온의 함량변화는 우유의 첨가량이 증가함에 따라 비례적으로 $Ca^{++}$, $K^{+}$, $Na^{+}$는 증가한데 반하여 $Mg^{++}$, Fe$^{++}$는 감소하였다. 관능평가에서 색감은 우유의 첨가량이 증가할수록 식감과 냄새 그리고 맛은 30~40%의 우유 첨가에서 평점이 높았다. 함황아미노산의 함량은 우유 10, 20, 30, 40, 50% (v/v) 합한 경우 각 대조구(0%)에 대하여 1.51, 1.58, 1.67, 1.85, 1.95배 증가되었다. 즉 두유에 우유를 첨가하여 두부를 만들 경우 기존 두부의 품질에 큰 변화없이 두부의 단백질이 강화되었고 부수적으로 맛도 구수하게 향상되는 결과를 얻었다.

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CA저장을 위한 폴리이미드 막 시스템의 질소-산소 분리특성 (Nitrogen-Oxygen Separation Characteristics by Polyimide Membrane System for Controlled Atmosphere Storage)

  • 이호원;현명택;고정삼
    • 한국식품저장유통학회지
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    • 제5권3호
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    • pp.239-246
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    • 1998
  • 폴리이미드 고분자막을 사용한 질소부하 장치를 설계하고, 이를 CA 저장에 활용하기 위한 기술적 기본 데이터를 제시하였다. 순수 산소 및 질소의 투과특성은 dual-mode sorption 모델로서 설명될 수 있었으며, 공기 중의 산소의 투과율은 공기 중에 존재하는 질소의 영향으로 순수 산소의 투과율에 비해 크게 감소하나, 공기 중의 질소의 투과율은 산소의 영향으로 순수 질소의 투과율에 비해 증가하였다. 수행한 압력 및 온도 범위 내에서 이상분리인자는 5에서 6 사이의 값을 나타내었으며, 공기의 분리인자는 이상분리인자보다 작은 값을 나타내었다. 산소의 투과활성화에너지가 질소의 투과 활성화에너지보다 커서 이상분리인자는 온도가 증가함에 따라 증가하였다. 제품회수율이 증가함에 따라 농축부의 질소농도는 급격히 감소하였으며, 이로부터 제품회수율은 CA 저장고에서 요구되는 질소농도를 얻기 위한 주요 조업인자임을 알 수 있었다. Polyimide 막 시스템을 이용한 CA 저장 공정의 가상적인 모형을 설정하고, 시스템의 조업시간에 따른 저장고의 질소농도를 예측할 수 있는 관계식을 제시하였다.

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까치복 알의 지질 조성에 관한 연구 (Studies on Lipid Composition of Egg from Fugu xanthopterus)

  • 이민경;조용계
    • 한국식품영양학회지
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    • 제10권2호
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    • pp.208-212
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    • 1997
  • 까치복(Fugu xanthopterus) 알을 총지질 함량과 silica gel column chromatography로 조성별 분획하여 얻은 TG, 인지질, wax ester의 지방산과 wax ester의 알코올 조성을 GC로 분석하였다. 까치복 알의 총지질 함량은 12.6%였고 알 지질의 분획별 함량에서는 TG가 59.37%로 가장 많았고 인지질이 15.46%였으며 wax ester가 6.9%로 나타났다. 까치복 알의 지방산 조성은 각 분획마다 DHA의 함량이 높게 나타났으며 DPA, EPA의 함량도 높았음을 알 수 있었고 인지질 분획에서는 DHA가 19.3%로 가장 높게 나타났다. 포화 지방산, 모노 불포화 지방산, 고도 불포화 지방산의 함량은 각각 35.3, 27.4, 37.3%로 고도 불포화 지방산의 함량이 높게 나타났음을 알 수 있었다. 한편 까치복 알의 wax ester의 지방산 분획에서는 포화 지방산, 모노 불포화 지방산, 고도 불포화 지방산이 5.8, 47.0, 47.4%로 나타났는데, 알코올 분획에서는 C16:0가 62.6%로 높은 함량을 나타내어 포화 알코올이 86.3%로 가장 많았음을 알 수 있었으며 고도 불포화 알코올은 1.2%의 아주 낮은 함량을 보인 것이 특징적이었다.

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Inhibitory effects of calcium against intestinal cancer in human colon cancer cells and $Apc^{Min/+}$ mice

  • Ju, Jihyeung;Kwak, Youngeun;Hao, Xingpei;Yang, Chung S.
    • Nutrition Research and Practice
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    • 제6권5호
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    • pp.396-404
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    • 2012
  • The aim of the study was to investigate the inhibitory effects of calcium against intestinal cancer in vitro and in vivo. We first investigated the effects of calcium treatment in HCT116 and HT29 human colon cancer cells. At the concentration range of 0.8-2.4 mM, calcium significantly inhibited cell growth (by 9-29%), attachment (by 12-26%), invasion (by 15-31%), and migration (by 19-61%). An immunofluorescence microscope analysis showed that the treatment with calcium (1.6 mM) for 24 h increased plasma membrane ${\beta}$-catenin but decreased nuclear ${\beta}$-catenin levels in HT29 cells. We then investigated the effect of dietary calcium on intestinal tumorigenesis in $Apc^{Min/+}$ mice. Mice received dietary treatment starting at 6 weeks of age for the consecutive 8 weeks. The basal control diet contained high-fat (20% mixed lipids by weight) and low-calcium (1.4 mg/g diet) to mimic the average Western diet, while the treatment diet contained an enriched level of calcium (5.2 mg calcium/g diet). The dietary calcium treatment decreased the total number of small intestinal tumors (by 31.4%; P < 0.05). The largest decrease was in tumors which were ${\geq}$ 2 mm in diameter, showing a 75.6% inhibition in the small intestinal tumor multiplicity (P < 0.001). Immunohistochemical analysis showed significantly reduced nuclear staining of ${\beta}$-catenin (expressed as nuclear positivity), but increased plasma membrane staining of ${\beta}$-catenin, in the adenomas from the calcium-treated groups in comparison to those from the control group (P < 0.001). These results demonstrate intestinal cancer inhibitory effects of calcium both in human colon cancer cells and $Apc^{Min/+}$ mice. The decreased ${\beta}$-catenin nuclear localization caused by the calcium treatment may contribute to the inhibitory action.

알팔파 사포닌이 흰쥐 혈청 콜레스테롤 농도에 미치는 영향 (Effect of Alfalfa Saponin on the Serum Cholesterol Level in Rats)

  • 조영수;굴미강남
    • 한국식품영양과학회지
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    • 제18권4호
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    • pp.430-434
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    • 1989
  • 알팔파 뿌리에서 사포닌을 추출, 단리하여서, 콜레스테롤 무첨가식이에 0.2% 및 0.4% 첨가군과 콜레스테롤 첨가식이에 0.4% 군을 설정, 80g 전후의 Wistar계 흰쥐수컷에 21일간 급여하였다. 그 결과 혈청지질과 분중지질 배설량을 측정한 결과 다음과 같다. 1. 알팔파 사포닌중 Medicagenic acid함량은 36.4%이었다. 2. 알팔파 사포닌 급여에 의하여 분중 콜레스테롤 배설량은 증가하였으나, 담즙산은 증가하지 않았다. 3. 알팔파 사포닌은 콜레스테롤 첨가, 무첨가식이에서 0.4%첨가에 의해 혈청콜레스테롤 저하작용을 나타내었지만, 0.2% 첨가에서는 관찰되지 않았다. 4. 사포닌의 혈청 콜레스테롤 저하작용은 소화관내에서의 콜레스테롤 흡수저하에 기인되는 것으로 여겨진다.

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