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http://dx.doi.org/10.13103/JFHS.2019.34.6.557

Comparison of DNA Extraction Methods for the Detection of Foodborne Pathogenic Bacteria from Livestock Manure Composts  

Kim, Sung-Youn (Division of Safety Analysis, Experiment & Research Institute National Agricultural Products Quality Management Service)
Seo, Dong-Yeon (Division of Safety Analysis, Experiment & Research Institute National Agricultural Products Quality Management Service)
Moon, Ji-Young (Division of Safety Analysis, Experiment & Research Institute National Agricultural Products Quality Management Service)
Publication Information
Journal of Food Hygiene and Safety / v.34, no.6, 2019 , pp. 557-561 More about this Journal
Abstract
This study investigated the efficacy of DNA extraction methods for real-time PCR detection of foodborne pathogenic bacteria in livestock manure composts. Livestock manure composts were inoculated with Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus and incubated in enrichment broth. For DNA extraction, enriched samples were treated following boiling method, by chloroform, C18 powder, and proteinase K. As a result, 4 species of bacteria were detected by real-time PCR when subjected to boiling for 30 min and treated with proteinase K. These results suggest that detection of foodborne pathogens by real-time PCR from livestock manure composts could be applicable using effective DNA extraction methodology such as the boiling method or proteinase K.
Keywords
Livestock manure composts; Real-time PCR; Boiling method; Chloroform; Proteinase K;
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