• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1690-1698
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• 2006

In order to investigate the effect of dissolved oxygen (DO) level on AVM $B_{1a}$ production by a high yielding mutant of Streptomyces avermitilis, five sets of bioreactor cultures were performed under variously controlled DO levels. Using an online computer control system, the agitation speed and aeration rate were automatically controlled in an adaptive manner, responding timely to the oxygen requirement of the producer microorganism. In the two cultures of DO limitation, the onset of AVM $B_{1a}$ biosynthesis was observed to casually coincide with the fermentation time when oxygen-limited conditions were overcome by the producing microorganism. In contrast, this phenomenon did not occur in the parallel fermentations with DO levels controlled at around 30% and 40% throughout the entire fermentation period, showing an almost growth-associated mode of AVM $B_{1a}$ production: AVM $B_{1a}$ biosynthesis under the environments of high DO levels started much earlier than the corresponding oxygen-limited cultures, leading to a significant enhancement of AVM $B_{1a}$ production during the exponential stage. Consequently, approximately 6-fold and 9-fold increases in the final AVM $B_{1a}$ production were obtained in 30% and 40% DO-controlled fermentations, respectively, especially when compared with the culture of severe DO limitation (the culture with 0% DO level during the exponential phase). The production yield ($Y_{p/x}$), volumetric production rate (Qp), and specific production rate (${\bar{q}}_p$) of the 40% DO-controlled culture were observed to be 14%, 15%, and 15% higher, respectively, than those of the parallel cultures that were performed under an excessive agitation speed (350 rpm) and aeration rate (1 vvm) to maintain sufficiently high DO levels throughout the entire fermentation period. These results suggest that high shear damage of the high-yielding strain due to an excessive agitation speed is the primary reason for the reduction of the AVM $B_{1a}$ biosynthetic capability of the producer. As for the cell growth, exponential growth patterns during the initial 3 days were observed in the fermentations of sufficient DO levels, whereas almost linear patterns of cell growth were observed in the other two cultures of DO limitation during the identical period, resulting in apparently lower amounts of DCW. These results led us to conclude that maintenance of optimum DO levels, but not too high to cause potential shear damage on the producer, was crucial not only for the cell growth, but also for the enhanced production of AVM $B_{1a}$ by the filamentous mycelial cells of Streptomyces avermitilis.

• Journal of Broadcast Engineering
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• v.19 no.4
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• pp.521-532
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• 2014

ATSC-M/H based hybrid 3DTV, which is one of the service compatible 3DTV system, has considerable quality gap between the left and right views. And CRA(Conditional Replenishment Algorithm) has been proposed to deal with the issue of resolution mismatch and improve the visual quality. In CRA, the disparity vectors of stereoscopic images are estimated. The disparity compensated left view and simply enlarged right view are compared and conditionally selected for generating the enhanced right view. In order to implement CRA, a fast algorithm is strongly required because the disparity vectors need to be obtained at every layer and the complexity of CRA is quite high. In this paper, we adopted SDSP(Small Diamond Search Pattern) instead of full search and predicted the initial position of search pattern by examining the spatio-temporal correlation of disparity vectors and also suggested the SKIP mode to limit the number of processing units. The computer simulation showed that the proposed fast algorithm could greatly reduce the processing time while minimizing the quality degradation of reconstructed right view.

• The Korean Journal of Pharmacology
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• v.24 no.1
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• pp.31-42
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• 1988

The effect of Panaxadiol(PD), which is an active component of Korean Ginseng Saponins, on the secretion of catecholamines (CA) from the rabbit adrenal gland and its mode of action were investigated in the present study. $PD(400{\mu}g)$ increased significantly the secretion of CA from the isolated perfused rabbit adrenal gland. PD-induced secretion of CA was reduced markedly by treatment of atropine, CA secretion induced by Ach or PD was potentiated significantly by physostigmine-treatment. Chlorisondamine did inhibit CA secretion of PD or Ach. Perfusion of $PD(400{\mu}g)$ for 30 min enhanced the secretory activity of CA by Ach. Ouabain weakened the secretory response induced by PD but rather enhanced the response by Ach. Adenosine-treatment resulted in marked enhancement of CA secretion by PD or Ach, Pefusion with $Ca^{2+}-free$ Krebs containing EGTA (5 mM) for about 30 min totally blocked secretory effect induced by Ach and also weakened that by PD. From the above experimental results, it is suggested that PD causes secretion of catecholamines from the rabbit adrenal gland by a calcium-dependent exocytotic mechanism. The secretory effect of PD is due to the stimulation of cholinergic muscarinic and nicotinic receptors present in the adrenal gland and partly to a direct action on the chromaffin cell itself.

• Journal of Biomedical Engineering Research
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• v.31 no.3
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• pp.217-226
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• 2010

The lower limb orthosis with a pneumatic rubber actuator, which is intended for the assistance and the enhancement of muscular activities of lower limbs was developed in this study. Compared to other knee extension assistive devices being developed by other researchers, our device is designed especially for the elderly people and intended only for slight assistance so that the subjects can keep their muscular strength. For the effectiveness of system, muscular activities of major muscles in lower limbs during sit-to-stand (STS) and squat motion were measured and analyzed. Subjects were performed the STS and squat motion with and without lower limb orthosis. We made comparison muscular activities between with and without lower limb orthosis. Lower limb orthosis was controlled using muscular stiffness force feedback that is controlled by muscular activities of the measured muscle from force sensor. For analysis of muscular activities, electromyography of the subjects was measured during STS and squat motion, and these were measured using MP 150(BIOPAC Systems, Inc.). Muscles of interest were rectus femoris(RF), vastus lateralis(VL), vastus medialis(VM) and vastus intermedius(VI) muscles in lower limbs of the right side. A biodex dynamometer was used to measure the maximal concentric isokinetic strength of the knee extensors of wearing and not wearing orthosis on right side. The test were performed using the concentric isokinetic mode of test with the velocity set at 60°/s for muscles around the knee joints. The experimental result showed that muscular activities in lower limbs wearing orthosis using muscular stiffness force of a vastus medialis muscle was reduced and knee extension torque of an knee joint wearing lower limb orthosis was increased. With this, we confirmed the effectiveness of the developed lower limb orthosis.

• Journal of Korean Society of Steel Construction
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• v.20 no.2
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• pp.333-345
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• 2008

concrete deck bridges are increasingly aplied to twin- girder bridges and open-stel box girder bridges.One of the most dificult isues in the design of shear conect ors is the mater of achieving ful composite action. Many connectors in smal area require a significant section los of precast decks resulting in difficult reinforcement details. In this closer spacing than the required minimum spacing in the design codes was evaluated through static tests. Test results s howed that the ultimate strength decreased as the conector spacing was reduced. The strength enhancement was observed due to aditional reinforcement for precast slabs or for shear pockets. Thus, the design of group stud shear connection needs to anticipate failure modes and the conector failure should be induced. Based on the test results, an empirical equation consi dering stud spacing was proposed to evaluate the ultimate strength of group stud shear conection. Fatigue tests showed n o reduction in fatigue life of the group stud shear conection in the range of this research. Details of the precast decks wer e enhanced using the findings of the study.

• Journal of the Korea Concrete Institute
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• v.19 no.2
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• pp.135-144
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• 2007

Recently, fiber-reinforced plastic(FRP) wraps are blown as an effective material for the enhancement and rehabilitation of aged concrete structures. The purpose of this investigation is to experimentally investigate behavior of concrete cylinder wrapped with FRP materials. Experimental parameters include compressive strength of concrete cylinder, FRP material, and confinement ratio. This paper presents the results of experimental studies on the performance of concrete cylinder specimens externally wrapped with aramid, carbon and glass fiber reinforced Polymer sheets. Test specimens were loaded in uniaxial compression. Axial load, axial and lateral strains were investigated to evaluate the stress-strain behavior, ultimate strength ultimate strain etc. Test results showed that the concrete strength and confinement ratio, defined as the ratio of transverse confinement stress and transverse strain were the most influential factors affecting the stress-strain behavior of confined concrete. More FRP layers showed the better confinement by increasing the compressive strength of test cylinders. In case of test cylinders with higher compressive strength, FRP wraps increased the compressive strength but decreased the compressive sham of concrete test cylinders, that resulted in prominent brittle failure mode. The failure of confined concrete was induced by the rupture of FRP material at the stain, being much smaller than the ultimate strain of FRP material.

• Journal of Dairy Science and Biotechnology
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• v.33 no.1
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• pp.27-34
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• 2015

The aim of this study was to optimize a simple, fast, and economic analytical method for the simultaneous determination of various pesticides (aldrin, p,p'-DDT, o,p'-DDT, p,p'-DDE, ${\alpha}$-endosulfan, ${\beta}$-endosulfan, dieldrin, heptachlor, permethrin, chlordane, deltamethrin, diazinon, bifenthrin, methoprene, propargite, fenpropathrin, cypermethrin, fenvalerate, and fenpropathrin) in milk by using dispersive solid phase extraction (SPE). In this study, two different extraction methods (low temperature cleanup and liquid-liquid partitioning), which were followed by a cleanup process based on dispersive-SPE, were evaluated and compared for the 19 pesticides. The results for all the pesticides were determined by gas chromatography mass spectrometry (GC/MS) with selected-ion monitoring mode, and the matrix effect of the method was evaluated. Comparison of these approaches yielded higher and more consistent recoveries of most pesticides at fortification levels of $1{\mu}g/mL$ using low-temperature fat precipitation, followed by cleanup process based on dispersive-SPE with PSA and C18 as sorbents, than other preparation process. The relative standard deviation was <20 % and the combination of this method were very effective for the cleanup.

• The Korean Journal of Mycology
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• v.40 no.4
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• pp.224-228
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• 2012

In order to screen interactor(s) of the Aspergillus nidulans ${\alpha}$-COP of COPI vesicle, we performed the yeast two hybrid screening by using the gene for A. nidulans ${\alpha}$-COP as a bait and identified ${\varepsilon}$-COP of the COPI vesicle as an interacting protein. The A. nidualns gene for the ${\varepsilon}$-COP was designated $aneA^+$ ($\underline{A.}$ $\underline{n}$idulans $\underline{e}$psi-lone-COP), which encoded 296 amino acid residues with high level of identity with orthologs from other fungi. Domain analyses with yeast two-hybrid system suggested that the interaction between ${\alpha}$-COP and ${\varepsilon}$-COP relied on the C-terminus of both proteins, and that the N-terminal WD domian of ${\alpha}$-COP and the TPR region of ${\varepsilon}$-COP were not essential but required for the enhancement of the interaction. These results indicate that the interaction mode between ${\alpha}$-COP and ${\varepsilon}$-COP of COPI vesicle is evolutionarily well conserved in eukaryotes.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.4
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• pp.475-483
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• 2012

This paper presents new type of the hybrid supply modulator for the next reconfigurable transmitters. The efficiency of the hybrid supply modulator is one of the most important performance. For enhancement the efficiency, multi-switching structure in the hybrid supply modulator is employed. Additionally, input envelope signal sensing stage is employed for implementation multi-mode operation. To compare the performance of the proposed hybrid supply modulator, the conventional hybrid supply modulator is also designed. The measured efficiency of the proposed hybrid supply modulator is 85 %/84 %/79 % for EDGE/WCDMA/LTE signals which have 384 kHz/3.84 MHz/5 MHz bandwidth, respectively. The efficiency of the proposed hybrid supply modulator is higher than the conventional hybrid supply modulator. Therefore, this structure shows good candidate for the reconfigurable transmitters.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.49 no.2
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• pp.149-156
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• 2012

Support vector machines (SVMs) are well known for their pattern recognition capability, but proper care should be taken to alleviate their inherent implementation cost resulting from high computational intensity and memory requirement, especially in embedded systems where only limited resources are available. Since the memory requirement determined by the dimensionality and the number of support vectors is generally too high for a cache in embedded systems to accomodate, frequent accesses to the main memory occur inevitably whenever the cache is not able to provide requested data to the processor. These frequent accesses to the main memory result in overall performance degradation and increased energy consumption because a memory access typically takes longer and consumes more energy than a cache access or a register access. In this paper, we propose a technique that reduces the number of main memory accesses by optimizing the data access pattern of the SVM-based classifier in such a way that the temporal locality of the accesses increases, fully utilizing data loaded into the processor chip. With experiments, we confirm the enhancement made by the proposed technique in terms of the number of memory accesses, overall execution time, and energy consumption.