• Journal of Navigation and Port Research
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• v.30 no.1 s.107
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• pp.97-104
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• 2006

Recently the first stage qf construction for Busan new port emerged over the sea surface at the north container terminal site. With this, there are lot of discussions and debates on increasing the water depth at the approaching channel and mooring basin from the existing 15m to 18m by dredging work in order to be able to serve 12,000TEU containership, and at the same time, correction to the reclamation plan of hinterland at the part of Undong Bay qf the new port site. Since the attack of typhoon 'Maemi' in 2003, it is expected that the design wave parameters for coastal and harbor structures in this area would be somewhat changed and so the extreme wave condition at each terminal and tranquility of berthing area does, and therefore, it is necessary to analyze the tranquility at each berth Hence in this study, we constructed a wave model for these conditions and performed simulation together with the circulation model simulation, compared with the field data collected The result showed the increase of the harbor response throughout the basin but not severe condition However, a certain location needs to be prepared for the rough sea condition when a severe typhoon hit the site.

• 한국지구물리탐사학회:학술대회논문집
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• 2005.09a
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• pp.155-175
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• 2005

Soil and rock dynamic properties such as shear wave velocity (VS), compressional wave velocity (VP) and corresponding Poisson's ratio ( v ) are very important geotechnical parameters in predicting deformational behavior of structures as well as practicing seismic design and performance evaluation. In an effort to measure the parameter efficiently and accurately, various bore-hole seismic testing techniques have been, thus, developed and used during past several decades. In this study, cross-hole seismic testing technique which is known as the most reliable seismic method was adopted for obtaining geotechnical dynamic properties. To perform successfully the cross-hole test for rock as well as soil layers regardless of the ground water level, spring-loaded source which impact laterally a subsurface ground in vertical bore-hole was developed and applied at three study areas, which contain four sites composed of two existing port sites and two new LNG storage facility sites. The geotechnical dynamic properties such as VS, VP and v with depth were efficiently determined from the laterally impacted cross-hole seismic tests at study sites, and were provided as the fundamental parameters for the seismic performance evaluation of the existing ports and the seismic design of the LNG storage facilities.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• Tunnel and Underground Space
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• v.14 no.6 s.53
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• pp.440-449
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• 2004

Rock mass includes natural discontinuities such as joints and faults during its formation. Discontinuities are also referred as planes of weakness because of their weak mechanical characteristics. In the design of underground structures, it is necessary to consider the properties of discontinuities to insure the stability. During the excavation of a tunnel, these discontinuities have to be identified as early as possible so that proper change in excavation method or support design can be made accordingly. The excavation of the tunnel in a stable rock mass causes a 3-dimensional arching effect around the excavation face. It was revealed by previous studies that the existence of a weak zone or a fault zone ahead of tunnel foe induces a typical displacement tendency of convergence. For better understanding of the meaning of influence/trend lines of various displacement components, three-dimensional numerical analyses were conducted while varying deformation moduli, thicknesses and orientations of discontinuities. Numerical results showed that the changes in influence/trend lines of various displacement components were very similar to those by measurements. The discrepancies from the expected values were dependent on the physical properties, thicknesses and orientations of discontinuities.

• Tunnel and Underground Space
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• v.18 no.1
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• pp.20-32
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• 2008

The study shows a method called a square-inventory method, which is a better and faster method than scanline survey and window method for an analysis of slope stability. The study area is located in the Changri area, Boeun-Gun, Chungbuk, and consists of many formations of the Okcheon Supergroup. Various types of failure are observed from the phyllite including the rocks in the study area. The physical properties of meta-sedimentary rocks are that minerals of the rocks are composed of microcrystalline quartz and sericite, which are arranged parallel to bedding (or schistosity) and crenulation cleavage. Therefore, such properties affect geotechnical ones of the rock. The slope stability are analyzed by selecting 3 areas, each of which are divided into 2 or 3 slopes of $1m{\times}1m$ area that represent each of 3 investigation sites. The possibility of wedge and toppling failure is very high in all 3 areas by using square-inventory method. Although possibility of plane failure is weak in the investigation site 2, the plane failures are frequently found from the slope of site 2. The bedding (or schistosity) plane and cleavage, another types of discontinuity coexist in meta-sedimentary rocks uulike igneous rocks, and therefore are important factors to be considered together with joint structures in th ε analysis of slope stability.

• Tunnel and Underground Space
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• v.18 no.1
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• pp.80-89
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• 2008

The influence of in-situ rock stress on the stability of an underground rock structure increases as the construction depth become deeper and the scale of a rock structure become larger. In general, hydraulic fracturing stress measurement has been performed in the surface boreholes of the target area at the design stage of an underground structure. However, for some areas where the high horizontal stresses were observed or where the overstressed conditions caused by topographical and geological factors are expected, it is desirable to conduct additional in-situ stress measurement in the underground construction site to obtain more detailed stress information for ensuring the stability of a rock structure and the propriety of current design. The study area was a construction site for the additional underground oil storage facility located in the south-east part of OO city, Jeollanam-do. Previous detailed site investigation prior to the design of underground structures revealed that the excessive horizontal stress field with the horizontal stress ratio(K) greater than 3.0 was observed in the construction area. In this study, a total of 13 hydraulic fracturing stress measurements was conducted in two boreholes drill from the two water tunnel sites in the study area. The investigation zone was from 180 m to 300 m in depth from the surface and all of the fracture tracing works were carried out by acoustic televiewer scanning. For some testing intervals at more than 200 m ind depth from surface, the high horizontal stress components the horizontal stress ratio(K) greater than 2.50 were observed. And the overall investigation results showed a good agreement with the previously performed test.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.5 no.2
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• pp.11-18
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• 1985

A row of bored piles has been used in several excavation works to retain the earth. This excavation bracing system has much effect on low-vibration and low-noise during construction. The system is also effective to provide protection to the adjacent existing ground and structures. For the purpose of establishment of a logical design method for the bored piles, first, a theoretical equation to estimate the resistance of piles is derived. Because arching action of soils between piles is considered in the equation, the characteristics of soils and the installation condition of piles would be considered logically from the beginning. Then a method is investigated to decide the interval ratio of piles. According to the method, the interval between piles can be decided from the information of the Peck's stability number, the coefficient of lateral earth pressure and the internal friction angle of soil. Finally, a design method is presented for the bored piles used for excavation work. In the presented design method, such factors as depth of excavation, pile diameter, interval between piles, pile length below bottom of excavation and pile stiffness, can be selected systematically.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.5 no.2
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• pp.109-120
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• 1985

This thesis is a study on multiple close range photogrammetry, and the purpose of this study is to develop the most accurate adjustment method of three dimensional object coordinates. This was achieved by comparing the standard errors of actual data to the computed values from 2 photos and multiple photos. The conventional methods for multiple photos have been analyzed by using geometric model formation. But in this study, the equation of collinearity condition which has been applied to aerial photogrammetry was derived to be a basic principle of close range photogrammetry, and the algorithm for analyzing multiple photos was developed using simultaneous bundle adjustment. The method used in this study, showed more homogeneous accuracy in coordinate and more consistent variance of error than those of conventional methods. It was found that the cases using 3, 4, and 5 photos were more accurate than using 2 photos; the accuracies were improved to 15%, 35%, and 50%, for each case. Thus this study is expected to be useful in measuring the geometry of historic monuments and other structures requiring high accuracy. Also the combined case of multiple photos is considered to be effective for the precise analysis of the objects which are difficult to measure for obstacles.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.5 no.2
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• pp.91-99
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• 1985

The acceleration levels and durations of seismic inputs for nuclear power plant design are surveyed. Among those inputs, two artificial acceleration time histories with same acceleration level and duration are selected and their characteristics are studied by calculating response spectra and spectrum intensity. The selected time histories which have the duration of 24 sec. satisfy the design response spectra of US Nuclear Regulatory Commission Regulatory Guide 1. 60. One of the selected time histories is improved to have the duration of 15 sec. without significant changes in the other characteristics. A case study of a plane model with 3 lumped masses is done using three time histories, i.e, two selected and one improved time histories. It is found that the improved curve gives almost the same results as the original one and reduces the computer time by about half, whereas two selected time histories give the results with same trend but much different magnitudes each other. It is claimed, however, that the improved time history is not the optimal one, but very economical in practical applications.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.11
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• pp.1381-1389
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• 2012

The object of this study considers corrosion fatigue improvement of 7075-T6 aluminum by using shot peening treatment on 3.5% NaCl solution at room temperature. Aluminum alloy is generally used in aerospace structural components because of the light weight and high strength characteristics. Many studies have shown that an aluminum alloy can be approximately 50% lighter than other materials. Mostly, corrosion leads to earlier fatigue crack propagation under tensile conditions and severely reduces the life of structures. Therefore, the technique to improve material resistance to corrosion fatigue is required. Shot peening technology is widely used to improve fatigue life and other mechanical properties by induced compressive residual stress. Even the roughness of treated surface causes pitting corrosion, the compressive residual stress, which is induced under the surface layer of material by shot peening, suppresses the corrosion and increases the corrosion resistance. The experimental results for shot peened specimens were compared with previous work for non treated aluminum alloy. The results show that the shot peening treatment affects the corrosion fatigue improvement of aluminum alloys and the induced compressive residual stress by shot peening treatment improves the resistance to corrosion fatigue.