• Journal of Hospice and Palliative Care
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• v.17 no.1
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• pp.10-17
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• 2014

Purpose: This study was conducted to examine understanding of the meaning of well-dying and types of such views held by medical practitioners and nursing students. Methods: The Q-methodology was used to analyze the subjectivity of each item. The P-sample was made up of 22 medical practitioners or nursing students. The P-sample was instructed to rate 33 statements using a 7-point scale to obtain forced normal distribution. They were asked to make extra comments on the statements that were placed on both ends of the distribution curve. The PC-QUANL Program was used for the factor analysis of the collected data. Results: The participants had three types of meaning of well-dying. Total variance explained by these types was 57.97%" where type 1 was "reality-oriented", type 2 "relationship-oriented" and type 3 "obeying-the-nature". Conclusion: The participants' subjective views on well-dying influences their medical practice on patients who are facing death. Therefore, medical practitioners should have profound insights concerning life and death. To that end, a training program is needed to help medical practitioners develop a proper view on well-dying by subjectivity type.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.3
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• pp.213-219
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• 1995

The author carried out an experiment to find out the response of Black porgy, Acanthopagrus schlegelii [Bleeker] to the color lights. The experimental tank ($360L{\times}50W{\times}55H$cm) was set up in a dark room. Six longitudinal sections with 60cm intervals are marked in the tank to observe the location of the fish. Water depth in the tank was kept 50cm level. Light bulbs of 20 W at the both ends of the tank projected the light horizontally into the tank. Two different colored filters were selected from four colors of red, blue, yellow, and white, and they were placed in front of the light bulbs to make different colors of light. Light intensity was controlled by use of auxiliary filters intercepted between the bulb and the filter. The fishes were acclimatized in the dark for 50 minutes before they were employed in the experiment. Upon turning on the light, the number of fish in each section was counted 40 times in 30 second intervals, and the mean of the number of fish in each section was given as the gathering rate of the fish. The colors favourited by the fish was found in order of blue, yellow, white and red in the day-time and blue, red, yellow and white at night. The difference of the average distribution on two different colors of light was 7.18%(0.35-19.55%), and the difference in the daytime(8.20%) was larger than at night (6.15%). The gathering rate of fish on illumination period didn't show the regular trend of increase and decrease, and was fluctuated with instability. The difference of the gathering rate on two different colors of light wasn't distinct, and the difference in the daytime was larger than at night.

• The Journal of the Korean Society for Microbiology
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• v.22 no.3
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• pp.209-218
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• 1987

Leptospira interrogans, the causative organism of leptospirosis, is characterized by a fine helical morphology, and the helix is almost always right-handed. However, one of the striking features of recent isolates of L. interrogans in Korea was the heterogeneity in their morphology. Even under optimal culture conditions($30^{\circ}C$, EMJH medium), rods, spiral forms with right or left-handed helices, and even spherical forms of L. interrogans were present. Although the literature notes the presence of left-handed helices, long rods, and spherical forms in cultures of L. interrogans isolates, little is known about the cause of this morphologic heterogeneity. In an attempt to answer this question, this study was initiated to examine the effects of culture conditions, especially temperature and medium, on the morphology of L. interrogans. Four temperatures($5^{\circ}C$, $15^{\circ}C$, $30^{\circ}C$, and $37^{\circ}C$) and two types of media(Fletcher and EMJH) were used; one strain from Korean isolates and L. interrogans serovar canicola obtained from the Pasteur Institute(Paris, France) were employed throughout the study. The findings are as follows: 1. The L. interrogans isolated in Korea(UM-19) had a larger cell diameter($0.25{\sim}0.30\;{\mu}m$: $0.10{\sim}0.15\;{\mu}m$), and helix diameter($0.10{\sim}0.60\;{\mu}m$: $0.10{\sim}0.15\;{\mu}m$) than that obtained from the Pasteur Institute, but they varied in their distances between the helices($0.31{\sim}1.00\;{\mu}m$: $0.50{\sim}0.70\;{\mu}m$). 2, When UM-19 was grown at $37^{\circ}C$ after months or longer preincubation at $5^{\circ}C$ or $15^{\circ}C$, the majority of the organisms were spiral forms; however, they became rods when subcultured at $30^{\circ}C$ or $37^{\circ}C$. No significant morphological differences were found between Fletcher and EMJH media. 3. When L. interrogans serovar canicola was subcultured more than ten times at $37^{\circ}C$, some of the organism lost their motility as well as the hooks at either one or both ends, but only in Fletcher medium. The number of variants increased with the frequency of subculturing. These findings suggested that L. interrogans strain (UM-19) is different, in their morphology, from that of the Pasteur Institute, and its various morphologies may represent stages of the life cycle and vary with incubation temperature.

• Journal of fish pathology
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• v.8 no.1
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• pp.37-46
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• 1995

The amino acid analysis of polyhedrin protein and nucleotide sequence of polyhedrin gene in H. cunea nuclear polyhedrosis virus (HcNPV) genome have been studied. Polyhedrin had three polypeptide bands in SDS - polyactylamide gel electrophoresis. The major polypeptide had a molecular weight of 25 kd. The polyhedrin was composed of 17 different amino acids. HcNPV DNA was digested with EcoRI restriction enzyme and hybridized with ($\alpha^{32}P$) -labelled AcNPV polyhedrin gene cDNA. The polyhedrin gene was located on the fragment of EcoRI-H. The EcoRI - H fragment containing polyhedrin gene was cloned into the EcoRI site of pUC8 vector which was confirmed with southern blotting, and the recombinant plasmid containg polyhedrin gene was designated as hPE-H. The promoter region of polyhedrin genomic DNA was sequenced. The sequences identified as the TATA box was found at the 5' flanking region of the polyhedrin genomic DNA approximately -79 bp upstream from the transcriptional start site. But CAAT-like box was not shown near the TATA-like box in the polyhedrin gene. Four tandem repeats with the sequence 5' -CTAATAT-3' and 5'-TAAATAA-3' were found between -141 and -108 or -83 upstream and -52 bp downstream from the translation start site. About -141 bp region upstream from the translational start site was highly AT (78%) rich. The coding region for the polyhedrin starts and ends with ATG and TAA, respectively.

• Journal of Gifted/Talented Education
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• v.20 no.3
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• pp.1005-1026
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• 2010

Here we live in a fast changing world. The information and communication technologies have been surprisingly developing and making progress of our society. One of the benefit about the information and communication technologies is an internet. We can't imagine our life without it and it finally ends up with causing internet addiction which is called side effect of it. Most of former studies were, however, about game addiction and psychological factors of indivisual. In fact, there was not enough studies about internet addiction of computer science gifted students. If we analize the difference between computer science gifted students and average students when it comes to factors of internet addiction, we would surely be able to find out the result which is very helpful for cause analysis and its remedy. This paper examined the risk and protection factors of the former studies and choose various factors through Delphi analysis by specialists of information and science gifted students. Comparative analysis of computer science gifted students and average students was done through the test tools. And picking up unique factors of computer science gifted students, this paper shows the implication that contributes to protection of internet addiction and its remedy.

• The KIPS Transactions:PartD
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• v.16D no.4
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• pp.583-594
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• 2009

Most of the SOA solutions applicable to businesses and organizations are taking a top-down methodology. It starts with an analysis of an organization's requirements, followed by definition of business models and identification of candidate services and ends with finding or developing required services. Challenges in adopting SOA while abandoning legacy systems involve time and cost required during the process. Many businesses and organizations want to gradually migrate into SOA while making the most of the existing system. In this paper, we propose A Method for Migration of Legacy System into Web Service(M-LSWS); it allows legacy system to be migrated into web service accessible by SOA and be used as data repositories. M-LSWS defines procedures for migration into reusable web services through analysis of business processes and identification of candidate services based on design specification and code of legacy system. M-LSWS aims to migrate of legacy system into web service appropriate for SOA. The proposed method consists of four steps: analysis of legacy system, elicitation of reusable service and its specification, service wrapping and service registration. Each step has its own process and guideline. The eligibility of the proposed method will be tested by applying the method to book management system.

• Korean Journal of Construction Engineering and Management
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• v.19 no.5
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• pp.3-9
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• 2018

Although Life Cycle Cost (LCC) must be evaluated by experts, sometimes it may not allow a sufficient time for even the experienced LCC expert to make rational decisions. Therefore, it often ends with relatively comparing the final numbers. We have broken down 110 technical proposals that are actually bade and accepted for large construction projects, and then have analyzed the uncertainty of Maintenance and Energy (M&E) cost during building life cycle, which turns out be the most volatile factor in uncertainty of LCC. Also we suggest "Value Engineering Index (VEI)" - the reduced M&E cost that is normalized by the reduced first cost. It is analyzed that the most uncertain factors of the M&E cost include repair and replacement term differing from each project, duplicated repair and replacement, non-standard repair items, and site-specific energy cost. Eventually we propose a VEI population with a mean of 1.38 and a standard deviation of 1.19, which is obtained by individually and exclusively applying the uncertain factors of the M&E cost to the 35 standard sample of technical proposals. The LCC evaluators may be able to use the VEI population as the benchmark to select the technical proposal with the most reasonable LCC among many others in two suggested manners; the one is to deterministically calculate the probability of single VEIs, and the other is to stochastically calculate the probability of the VEIs where uncertainty is quantified.

• Journal of English Language & Literature
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• v.54 no.2
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• pp.301-322
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• 2008

Pauline Hopkins's Of One Blood; Or, the Hidden Self was published in the Colored American Magazine during 1902-03. As a literary experimentalist and a political protester, Hopkins uses her fiction as a medium to overcome and ameliorate the violently racialized surroundings of the turn-of-the-century America. Having been faced with racist rhetorics and theories growing on biological differences between races, Hopkins must have felt an overwhelming urgency to challenge the heritage of slavery in American history. In order to speak out her political agenda in such a milieu, she needed a new setting as well as new narrative materials for the new era. She had to move the setting from America to Africa, the ancient utopian Ethiopia; her interest in the ancient African civilization reflects both a popular African-American vision of Africa and the movement of "black nationalism" of the time. She also needed materials from nineteenthcentury sciences, the newly evolving theories of psychology and mysticism (spiritualism/mesmerism), to explore the meaning of "the hidden self" which unfolds the complex nature of Hopkin's position on race, "blood," and African-American racial subjectivity. Hopkins in the novel explores not the color line but the bloodline. Tracing the horrific legacy of incest in the history of slavery, she attempts to redefine the true racial identity of African-Americans in America and to reconstruct their past, both family and race history. At the very center of her major tropes in the novel-such as "of one blood," "the hidden self," and incest-exists female body. Black female body, though it represents the violent site of sexual body (rape and incest) in slavery, ultimately becomes a vehicle to convey and preserve the truth of racial memory/past/history for African-Americans. As a conveyor of the past, black women not just connect the past and the present but also reawaken AfricanAmericans with the legacy of the African 'pure' bloodline. Hopkins's vision here necessitates the reevaluation of black women's role in family and history, heralding the 20th-century black feminine writing. With the major tropes, Hopkins clearly suggests that the blood of (African-)Americans is unrecognizably intermixed. Although the novel ends with ambivalence and without resolution on what Africa signifies, those tropes certainly offer her a vehicle for criticizing as well as for challenging the racial reality of America.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.9-20
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• 2012

Eugenol is a volatile compound synthesized by eugenol synthase in various plants and belongs to phenylpropene compounds. However, characteristics of eugenol synthase in tomato has not been known. Therefore, we cloned a full length cDNA of a putative eugenol synthase from tomato 'Micro-Tom' using rapid amplification of cDNA ends (RACE) technique and named a clone SlEGS. Open reading frame of SlEGS was 921bp long and its deduced amino acid sequence was 307bp. The BLAST analysis indicated that SlEGS shared high similarity with PhEGS1 (67.1%) and CbEGS2 (69.4%). Amino acid composition of SlEGS was determined by CLC genomics workbench tool and 3D structure of SlEGS was constructed by homology modeling using Swiss-PDB viewer and validated using PROCHECK and ProSA-web tool. In addition, the physiochemical properties of SlEGS was evaluated using ExPASy's ProtParam tool. Molecular weight was 33.93kDa and isoelectric point was 5.85 showing acidic nature. Other properties such as extinction coefficient, instability index, aliphatic index, and grand average hydropathy was also analyzed.

• Korean Journal of Legislative Studies
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• v.23 no.1
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• pp.113-149
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• 2017

This study aims to identify the micro-level, social psychological foundation of regionalism and analyze its change and continuity by comparing 1988, 2003, and 2016 survey results. Drawing on the theory of prejudice and social identity, it clarifies the concept of regionalism and examines its affective, behavioral, cognitive implications. In the empirical analysis, where it takes advantage of relevant questions of the same or similar wording in three nationally representative surveys, the study identifies the changes in regionalism at the individual level focusing on anti-Honam prejudice and discrimination and attribution of regional conflict. First, anti-Honam prejudice has been in decline nationally as well as regardless of where one has grown up, except for Daegu/Kyungpook area. Second, anti-Honam prejudice has been weakened among younger generations while regional party identification now affects the sentiment in the direction of regional cleavage overlapped with ideological leanings. Third, while most respondents do not experience explicit discrimination, Honam natives are still more likely to experience discrimination, especially identity and self-esteem related, due to his or her home town. Fourth, Honam natives have been more likely to attribute regional conflict to an external, structural factor like government economic policy and less likely to a subjective one like regional sentiment, which seems to be consistent with attributional attitudes of the victims of prejudice. The study ends with the discussion of how to reduce further anti-Honam prejudice, which includes contact hypothesis, recategorization, cross-categorization, and de-categorization.