• Mycobiology
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• v.39 no.2
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• pp.71-78
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• 2011

The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial ${\beta}$-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.373-378
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• 2021

In this study, endophytic fungi were isolated from the leaves of evergreen woody plants in Hansando Island in South Korea. The fungal isolates were identified based on morphological characteristics and phylogenetic analysis of the DNA sequences of the internal transcribed spacer and the large subunit regions of rDNA. It was confirmed that two endophytic fungal species, Muyocopron lithocarpi and Didymocyrtis cladoniicola, isolated in this study have not previously been reported in South Korea.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.20-20
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• 2015

Endophytic fungi are microorganisms inhabiting living plant tissues without causing apparent harm to the host. They are drawing increasing attention due to their ability to produce various bioactive compounds as well as their effects on host growth and resistance to biotic and abiotic stresses. As a first step to assess biodiversity of plant associated fungi in Korea and the following evaluation on diverse biological activities, we are collecting endophytic fungi from plant in wild followed by systematic long-term storage in liquid nitrogen. Molecular identification using ITS sequences was also incorporated for pure culture by hyphal tip isolation. As of April 2015, about 1,400 fungal strains had been isolated from about 170 plant taxa. Fungal isolates belonging to Pleosporales, Diaporthales, Glomerellales, Hypocreales, and Xylariales were the most abundant. These collections are being used for several complementary researches, including screening of isolates with novel bioactive compounds or conferring drought stress resistance, phylogenetic and genomic study. Genome sequencing was performed for 3 isolates, one Xylaria sp. strain JS573 producing griseofulvin, an antifungal compound, and two Fusarium spp. strains JS626 and JS1030, which are assumed to be new species found in Korea. More detailed analysis on these genomes will be presented. These collections and genome informations will serve as invaluable resources for identifying novel bioactive materials in addition to expand our knowledge on fungal biodiversity.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.113-117
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• 2016

The biodiversity of endophytic fungi on Thuja koraiensis in Mt. Hwaak, Seorak, and Hambaek, Korea was investigated. For the 202 isolates collected from the host trees, internal transcribed spacer rDNA region sequences-based analysis identified 32 taxa; 61.5% of the isolates belonged to Dothideomycetes, 27.0% belonged to Sordariomycetes, and 11.5% belonged to Leotiomycetes. This composition rate is somewhat different from that reported in previous studies for endophytic fungi inhabiting trees of the family Pinaceae. In particular, Phyllosticta spinarum in Dothideomycetes is a dominant species among the diverse endophytes of T. koraiensis. Therefore, further critical research is required for this species.

• The Korean Journal of Mycology
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• v.42 no.3
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• pp.239-242
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• 2014

In this study, endophytic fungi were isolated from leaves of four species of woody plants, Acer tagmentosum, Larix kaempferi, Abies holophylla, and Pinus koraiensis, on Mt. Hambaek, Gangwondo, Korea. The endophytic fungi were identified using morphological and sequences analysis of ITS regions. The fungal endophytes were identified as Talaromyces radicus, Myceliophthora verrucosa, Cryptosporiopsis diversispora, and Sphaerulina berberidis, which are the first record in Korea. The morphological and molecular phylogenetic characteristics of each strain were described.

• The Korean Journal of Mycology
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• v.48 no.3
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• pp.217-228
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• 2020

Endophytic fungi were isolated from the leaves of plants inhabiting the Hansando Island in Korea. We identified the fungal strains based on their morphological characteristics and on the phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and translation elongation factor 1- α regions. In this study, we isolated five endophytic fungi that have not been recorded in Korea, Arthrinium camelliaesinensis, Beltraniella humicola, B. portoricensis, Microxiphium theae, and Piceomphale pinicola. Their morphological characteristics as well as the results of molecular phylogenetic analysis are reported here.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.324-333
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• 2014

The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were measured and compared between PDA with and without 3% NaCl to determine their saline resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal transcribed spacer region sequences identified 58 species. Representatives of each major fungi phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have higher resistance to salinity and potentially have specific relationships with P. thunbergii.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.405-414
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• 2018

Endophytic fungi were isolated from the leaves of diverse plants inhabiting Jeju Island, Korea. The fungal isolates were identified through phylogenetic analyses incorporating nucleotide sequences derived from the internal transcribed spacer region, large subunit region of ribosomal DNA, and beta-tubulin gene. Our results identified six endophytic fungi previously unknown in Korea namely, Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, and Zalerion arboricola were identified. Here, we present their cultural and morphological characteristics and phylogenetic relationship.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.169-173
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• 2020

We report on an unrecorded endophytic fungus, Grovesiella abieticola (Zeller & Goodd.) M. Morelet & Gremmen isolated from Abies koreana of Mt. Halla in Jeju. In this study, we used a culture method to determine its conidia and compared relative species using an internal transcribed spacer (ITS) 1, 2, and 5.8S ribosomal DNA sequence. Thus, we present the cultural characteristics and morphology of G. abieticola in this paper.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.313-318
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• 2019

In the present study, we isolated endophytic fungal strains from the rhizoids of the moss Funaria hygrometrica. The isolated strains were identified based on morphological characteristics and analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequence regions. Consequently, we confirmed the presence of two endophytic fungal species, Curvularia protuberata and Didymella anserina, which have not been reported in Korea previously. Here, we describe the morphological characteristics and molecular analysis results of these fungal species.