• Endocrinology and Metabolism
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• 제33권4호
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• pp.435-444
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• 2018

The prevalence of chronic diseases including osteoporosis and sarcopenia increases as the population ages. Osteoporosis and sarcopenia are commonly associated with genetics, mechanical factors, and hormonal factors and primarily associated with aging. Many older populations, particularly those with frailty, are likely to have concurrent osteoporosis and sarcopenia, further increasing their risk of disease-related complications. Because bones and muscles are closely interconnected by anatomy, metabolic profile, and chemical components, a diagnosis should be considered for both sarcopenia and osteoporosis, which may be treated with optimal therapeutic interventions eliciting pleiotropic effects on both bones and muscles. Exercise training has been recommended as a promising therapeutic strategy to encounter the loss of bone and muscle mass due to osteosarcopenia. To stimulate the osteogenic effects for bone mass accretion, bone tissues must be exposed to mechanical load exceeding those experienced during daily living activities. Of the several exercise training programs, resistance exercise (RE) is known to be highly beneficial for the preservation of bone and muscle mass. This review summarizes the mechanisms of RE for the preservation of bone and muscle mass and supports the clinical evidences for the use of RE as a therapeutic option in osteosarcopenia.

• 분석과학
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• 제12권4호
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• pp.265-272
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• 1999

에스트로겐처럼 행동하여 호르몬 수용체와 결합하거나 세포의 신호전달 과정에 영향을 미침으로서 내분비계를 교란시킬 수 있는 환경 에스트로겐 19종 (phytoestrogen: 12종, mycoestrogen: 5종, synthetic estrogen: 2종)의 동시 프로필 분석을 시도하였다. Gas Chromatography/Mass Spectrometry (GC/MS)의 selected ion monitoring (SIM) 방법을 기본으로 하였으며, 액체-고체 추출, 효소 기수분해, 액체-액체 추출 그리고 trimethylsilyl (TMS)-ether 형태의 유도체화를 거치는 비교적 간편한 전처리 방법으로 47.6~99.5%의 회수율과 0.66~9.33%, 1.66~16.14%의 within-a-day 및 day-to-day 분석의 RSD 값을 얻었다. 이 방법을 적용하여 정상 성인 여성과 남성의 뇨 시료에 존재하는 대상 물질들의 농도범위를 결정함으로서 환경 에스트로겐의 영향을 평가하는데 기준이 될 수 있는 한국인 정상인 참고치를 설정하였다.

• 한국식품영양학회지
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• 제29권2호
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• pp.290-299
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• 2016

This study was conducted to determine the association between dietary calcium intake and biomarkers related to lipid and glucose metabolism and inflammation in Korean patients with type 2 diabetes. Seventy-five subjects (41 males, 34 females) were recruited from a group of patients who had visited the department of endocrine medicine. Data on anthropometric characteristics, clinical indices such as hemoglobin A1c and C-reactive protein (CRP), and dietary nutrient intakes were collected. Subjects were divided into three groups on the basis of their calcium intake [< EAR (below estimated average requirement), EAR-RNI (between EAR and recommended nutrient intake), > RNI (above RNI)]. Average calcium intake of < EAR, EAR-RNI, > RNI groups were $462.7{\pm}18.7$, $649.7{\pm}12.8$, and $895.7{\pm}21.7mg$, respectively. Energy intake was not different among groups but intakes of protein, total and saturated fatty acids were significantly higher in > RNI group than < EAR group. Analysis of covariance revealed that HDL cholesterol level was significantly higher in EAR-RNI group, as compared to < EAR group after adjustment with confounders such as age, sex, BMI and energy intake (p < 0.05). Levels of CRP and homeostasis model assessment 2-insulin resistance (HOMA2-IR) were significantly lower in EAR-RNI group. Total cholesterol level was higher in EAR-RNI and > RNI groups, although within the normal range. Our results suggest that dietary calcium intake may influence the levels of HDL-cholesterol, CRP and HOMA2-IR and subsequently, help management/treatment of type 2 diabetes patients.

• Clinical and Experimental Reproductive Medicine
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• 제49권2호
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• pp.135-141
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• 2022

Objective: This study investigated the impact of two stimulation protocols using highly purified human menopausal gonadotropin (HP-hMG) on the endocrine profile, follicular fluid soluble Fas levels, and outcomes of intracytoplasmic sperm injection (ICSI) cycles. Methods: This prospective clinical trial included 100 normal-responder women undergoing ovarian stimulation for ICSI; 55 patients received concomitant follicle-stimulating hormone (FSH) plus HP-hMG from the start of stimulation, while 45 patients received FSH followed by HP-hMG during mid/late follicular stimulation. The primary outcome was the number of top-quality embryos. The secondary outcomes were the number and percentage of metaphase II (MII) oocytes and the clinical pregnancy rate. Results: The number of MII oocytes was significantly higher in the concomitant protocol (median, 13.0; interquartile range [IQR], 8.5-18.0 vs. 9.0 [8.0-13.0] in the consecutive protocol; p=0.009); however, the percentage of MII oocytes and the fertilization rate were significantly higher in the consecutive protocol (median, 90.91; IQR, 80.0-100.0 vs. 83.33 [75.0-93.8]; p=0.034 and median, 86.67; IQR, 76.9-100.0 vs. 77.78 [66.7-89.9]; p=0.028, respectively). No significant between-group differences were found in top-quality embryos (p=0.693) or the clinical pregnancy rate (65.9% vs. 61.8% in the consecutive vs. concomitant protocol, respectively). The median follicular fluid soluble Fas antigen level was significantly higher in the concomitant protocol (9,731.0 pg/mL; IQR, 6,004.5-10,807.6 vs. 6,350.2 pg/mL; IQR, 4,382.4-9,418.4; p=0.021). Conclusion: Personalized controlled ovarian stimulation using HP-hMG during the late follicular phase led to a significantly lower response, but did not affect the quality of ICSI.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.112-112
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• 2002

Under normal conditions, women produce a single dominant follicle that participates in a single ovuation each menstrual cycle. But Polycystic ovary syndrome(PCOS) conditions, folliculogenesis does not proceed normally. This condition leads to the accumlation of large numbers of small graffian follicles in which the theca interstitial cells (TIC) produce abnormally large amounts of androgen. PCOS is probably the most common endocrine disorder, affecting women of reprodutive age with 5-10% prevalence estimate. Chronic anovulation, hyperandrogenism, hirsutism, obesity, infertility and polycystic ovaries are clinical hallmarks of women with PCOS. Its etiology remains unknown. To investigate the gene expression pattern of ovary in PCO-induced rat, we used cDNA expression analysis. Total RNA was extracted from the ovary of PCO-induced rat and reverse-transcribed in the presence of［$\alpha$$^{32}$P］-dATP Which were hybridized to Atlas$^{TM}$ Rat Toxicology 1.2 array (Clontech) representing approximately 1176 rat genes. We compared gene expression between ovary of pco-induced immature female rats and control. Differential gene expression profiles were revealed (LIFR-alpha, ADRA1A, Heat shock 90-kDa protein A, PDGFRA). Reverse transcription-polymerase chain reaction(RT-PCR) was used to validate the relative expression pattern obtained by the cDNA array. The precise relationship between the altered expression of genes and PCO is a matter of further investigation. This study was supported by Korea Science and Engineering Foundation(KOSEF)

• Journal of Animal Science and Technology
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• 제50권3호
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• pp.343-354
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• 2008

본 연구는 산란계의 육성기 제한급여가 체성장 특성 및 내분비 변화에 미치는 영향을 구명하고자 1일령 갈색산란계 Isa-brown 1,080수를 공시하여 시험을 실시하였다. 육성기 제한급여 처리내용으로는 자유채식, 6~18주령까지 제한급여, 12~18주령까지 제한급여를 각각 실시하였으며 모든 사료영양소 함량 및 단계별 사료급여는 NRC(1994)에 준하여 실시하였다. 육성기간동안 체중과 사료섭취량은 6~18주령 제한급여 처리구가 가장 낮았으나(P<0.05) 20주령에서 각 처리구간 유의적 차이는 나타나지 않았다. 소화기관 및 실질장기 발달은 처리구간에 뚜렷한 차이는 없었으나 육성기간동안 체중이 낮았던 제한급여 처리구에서 다소 높은 비율을 보였다.체조성은 지방 함량에서 처리구간 유의성이 나타났는데 자유급여 처리구에 비해 6~18주령 제한급여 처리구에서 육성기간동안 유의적으로 낮게 나타났다(P<0.05). IGF-Ⅰ는 육성기간동안 높은 수준을 보였으나 번식기관이 발달하는 시기부터 급격히 하락하여 낮은 수준으로 유지되면서, 사료섭취량에 민감한 반응을 보였다. estradiol은 6주령 이후 전체적으로 증가하는 양상을 보였는데 16~17주령 사이에 증가하는 양상을 보였으며 종료 시까지 높은 수준을 유지하였는데 처리구간에 유의성은 나타나지 않았다.이상의 결과에서 6~18주 제한급여 처리구가 육성기간동안 체중, 증체량 및 사료섭취량은 자유급여 처리구에 비해 낮았으며(P<0.05), 지방함량 또한 적게 나타났다(P<0.05). 따라서 육성기 제한급여를 육성초기에 실시할 경우 체내 대사에 있어 큰 영향을 미치지 않았으며 estradiol 수준 역시 안정적으로 나타났다. 따라서, 본 연구 결과는 육성기 제한급여가 산란계 성장에 있어서 내분비와 체성장 조성에 영향하지 않으며 또한, 최근 곡물가격의 상승으로 인한 사료가격의 상승에 대한 문제를 해결 할 수 있는 해결방안으로 충분한 가치가 있을 것으로 사료된다. 하지만, 좀 더 개선되어져야 할 부분이 많은 만큼 향후 지속적으로 제한 급여 수준 및 기간 등에 관한 세밀한 연구가 이루어져야 할 것으로 사료된다.(색인어:산란계, 제한급여, Insulin-like growth factor-Ⅰ, 체성분)

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.97-98
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• 2003

육성기 제한급이가 육성기 발육특성 및 기초 산란형태에 미치는 영향을 규명하기 위하여 갈색 산란계 1,080수를 공시하여 24주간 사양시험을 실시하였다. C는 자유채식을 시켰으며, T1은 6∼12주령까지 대조구 70％, 18주령까지 75％ 수준으로 급여하였고, T2는 12∼18주령까지 T1과 동일하게 급여하였다. 18주령 체중은 T1이 가장 낮게 나타났고 C와 T2는 비슷하게 나타났으나 20주령 체중은 처리간 모두 비슷하게 나타났다. 18주령까지 사료섭취량은 T1이 가장 적었으며 C가 가장 많았다. 초산일령은 C가 가장 빨랐지만 90％ 산란일정은 T1이 가장 빠른 것으로 나타났다. 연란과 쌍란 발생률은 C가 가장 많았고 T1이 가장 적은 것으로 나타났다. 이상과 같은 결과에서 육성 초기 사료 제한급이는 육성기 사료섭취량과 산란초기 연란 및 쌍란 발생률을 감소시키며 급격한 산란증가를 유도하는 것으로 나타났다.

• Clinical and Experimental Reproductive Medicine
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• 제32권2호
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• pp.155-164
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• 2005

Objective: This study was performed to investigate the effects of pioglitazone, an insulin sensitizing agent, on insulin resistance, ovarian function and intraovarian stromal blood flow in patients with polycystic ovarian syndrome (PCOS). Material and Methods: Thirty patients with PCOS, aged 18~34 years, were recruited. Criteria for diagnosis of PCOS were as defined in 2003 Rotterdam consensus. They were treated for 6 months with pioglitazone at a dose of 30 mg/day orally. The hormonal blood profile, fasting serum glucose levels, a glycemic response to 75 g oral glucose tolerance test (OGTT), and an ovarian stromal artery (OSA) blood flow were assessed at baseline and after 6 months of treatment. Results: Eighteen (60.0%) of 30 patients treated with pioglitazone demonstrated a spontaneous ovulation After pioglitazone treatment, fasting insulin concentrations, serum glucose levels after 75 g OGTT significantly decreased (p=0.001, p=0.04, respectively), and fasting glucose to insulin (G/I) ratio significantly increased (p<0.001). The pioglitazone treatment induced a significant reduction in serum LH, testosterone (T) and free T levels (p<0.001, p=0.02, p=0.002, respectively). The resistance index (RI) values of OSA significantly increased after treatment (p<0.001). In analyzing pioglitazone-treated patients according to their body mass index (BMI), nonobese group as well as obese group showed a significant improvement in fasting G/I ratio (p<0.01). The pioglitazone treatment induced a significant reduction in serum LH and free T levels in nonobese group (p<0.001, p<0.05, respectively) as well as obese group (p=0.001, p<0.05, respectively). The RI values of OSA significantly increased in both nonobese and obese groups after pioglitazone treatment (p<0.001, p=0.003, respectively). Conclusions: Pioglitazone could ameliorate the glycoinsulinemic metabolism, and this beneficial effects of this drug could improve the endocrine-reproductive condition associated with the decrease of ovarian stromal artery blood flow, in both nonobese and obese patients with PCOS.