Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Profiling of Urinary Environmental Estrogens by Gas Chromatography/Mass Spectrometry

GC/MS를 이용한 뇨 중 환경 에스트로겐들의 동시 프로필

  • Yang, Yoon-Jung (Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Lee, Seon-Hwa (Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Chung, Bong-Chul (Bioanalysis & Biotransformation Research Center, Korea Institute of Science and Technology)
  • 양윤정 (한국과학기술연구원 새체대사연구센터) ;
  • 이선화 (한국과학기술연구원 새체대사연구센터) ;
  • 정봉철 (한국과학기술연구원 새체대사연구센터)
  • Received : 1999.02.26
  • Published : 1999.08.25
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Abstract

A simultaneous profile analysis of 19 environmental estrogens, which act like estrogen and may effect the endocrine system by binding to hormone receptors or influencing cell signaling pathways, was attempted. The present method was based on the selected ion monitoring (SIM) mode of gas chromatography/mass spectrometry (GC/MS). It involves solid-liquid extraction, enzyme hydrolysis, liquid-liquid extraction and quantitative conversion into trimethylsilyl (TMS)-ether derivatives. Analytical recovery range was 47.6 ~ 99.5% and the RSD values of within-a-day and day-to-day test were 0.66 ~ 9.33%, 1.66 ~ 16.14%, respectively. The Korean reference values for the evaluation of environmental estrogen effects were established by this method.

에스트로겐처럼 행동하여 호르몬 수용체와 결합하거나 세포의 신호전달 과정에 영향을 미침으로서 내분비계를 교란시킬 수 있는 환경 에스트로겐 19종 (phytoestrogen: 12종, mycoestrogen: 5종, synthetic estrogen: 2종)의 동시 프로필 분석을 시도하였다. Gas Chromatography/Mass Spectrometry (GC/MS)의 selected ion monitoring (SIM) 방법을 기본으로 하였으며, 액체-고체 추출, 효소 기수분해, 액체-액체 추출 그리고 trimethylsilyl (TMS)-ether 형태의 유도체화를 거치는 비교적 간편한 전처리 방법으로 47.6~99.5%의 회수율과 0.66~9.33%, 1.66~16.14%의 within-a-day 및 day-to-day 분석의 RSD 값을 얻었다. 이 방법을 적용하여 정상 성인 여성과 남성의 뇨 시료에 존재하는 대상 물질들의 농도범위를 결정함으로서 환경 에스트로겐의 영향을 평가하는데 기준이 될 수 있는 한국인 정상인 참고치를 설정하였다.

Keywords

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