• 대한수의학회지
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• 제36권2호
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• pp.297-303
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• 1996

가물치의 위장관 내분비세포의 부위별 분포 및 출현빈도를 면역조직화학적으로 관찰하였던 바, secretin 면역반응세포들은 위의 분문부와 위저부의 상피 또는 장선부에서 소수로 출현하였다. Motilin과 GRP 면역반응세포는 장의 원위부와 위저부의 상피에서 극소수로, PYY 면역반응세포들은 유문맹낭부위와 장의 근위부에서 소수 또는 극소수로 각각 관찰되었다. Substance P 면역반응세포들은 위저부에서 장의 원위부에 걸쳐 소수 내지는 비교적 다수의 출현빈도를 보였으며 또한 장의 원위부 고유층에서는 신경세포가 양성반응을 보였다. 그러나 neurotensin, met-Enk, GIP 면역반응세포들은 전장관에서 관찰할 수 없었다.

• 대한수의학회지
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• 제35권3호
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• pp.417-425
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• 1995

두툽상어의 위장관 내분비세포의 부위별 분포, 출현빈도 및 세포의 종류를 밝히고자 면역조직화학적 방법으로 관찰하였던 바, 5종의 면역반응세포가 동정되었다. 5-HT 면역반응세포는 최고의 빈도로, somatostatin 면역반응세포는 소수의 위부위와 극소수의 장부위에 걸쳐 각각 전장관에서 관찰되었다. Glucagon과 BPP 면역반응세포는 십이지장과 직장을 제외하고 다양한 빈도로 소화관 전체에서 출현하였다. 한편 Gas/CCK 면역반응세포는 소장부위에서 국한하여 소수 분포하였다. 이상에서 두툽상어의 위장관 내분비세포는 부위별 분포에 있어서 다른 종과 유사하였으나, 출현빈도는 다소 낮게 나타났음을 알 수 있었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.183.2-184
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• 2003

Zinc is known to have an inhibitory effect on apoptosis and an antioxidative effect scavenging reactive oxygen species (ROS) under oxidative stress. We studied the influence of zinc on cadmium-induced apoptosis especially associated with ROS in MCF-7 human breast carcinoma cell line. For the determination of appropriate experimental concentration and time, we excecuted MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay and DNA fragmentation assay. (omitted)

• Clinical and Experimental Reproductive Medicine
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• 제46권3호
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• pp.99-106
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• 2019

Bisphenol A (BPA) is an endocrine-disrupting chemical that is capable of interfering with the normal function of the endocrine system in the body. Exposure to this chemical from BPA-containing materials and the environment is associated with deleterious health effects, including male reproductive abnormalities. A search of the literature demonstrated that BPA, as a toxicant, directly affects the cellular oxidative stress response machinery. Because of its hormone-like properties, it can also bind with specific receptors in target cells. Therefore, the tissue-specific effects of BPA mostly depend on its endocrine-disrupting capabilities and the expression of those particular receptors in target cells. Although studies have shown the possible mechanisms of BPA action in various cell types, a clear consensus has yet to be established. In this review, we summarize the mechanisms of BPA action in spermatozoa by compiling existing information in the literature.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2005년도 제48차 춘계 학술대회
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• pp.111-111
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• 2005

• 대한수의학회지
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• 제41권2호
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• pp.123-132
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• 2001

ICR 마우스 위장관 8개 부위(위저부, 유문부, 십이지장, 공장, 회장, 맹장, 결장 및 직장)에서 위장관내분비 세포의 부위별 분포 및 상대적 빈도를 somatostain, serotonin, glucagon, chloecystokinin(CCK)-8, secretin, pancreatic polypeptide(PP) 및 gastrin 등 총 7종류의 항혈청을 이용한 면역조직화학적 방법으로 관찰하였던 결과 somatostain, serotonin, glucagon, CCK-8, secretin, 및 gastrin 면역반응세포의 7종류의 내분비세포가 관찰되었다. 본 실험의 결과 장관부위에서는 주로 타원형 또는 방추형의 개방형 세포(open-typed cell)들이 관찰된 반면 위저부와 유문부에서는 주로 원형의 폐쇄형 세포(close-typed cell)을이 관찰되었다. 이들 면역반응세포들의 부위별 분포는 위장관 각 부위에 따라 매우 다양하게 관찰되었다. Somatostain 면역반응세포들은 대장을 제외한 위장관에서 전 부위에서 관찰되었고, serotonin 면역반응세포들은 전 위장관에 걸쳐 관찰되었으며, ICR 마우스에서 가장 높은 빈도를 나타내었다. Glucagon 면역반응세포들은 위저부와 직장에 국한되어 관찰되었으며, 각각 중등도 및 소수의 빈도를 나타내었다. CCK-8 면역반응세포들은 유문부, 십이지장 및 회장에서 각각 다수, 중등도 및 극소수의 빈도로 관찰되었다. 한편 secretin 면역반응세포들은 각각 소수 및 극소수의 빈도로 십이지장과 회장에 국한되어 출현하였고, gastrin 면역반응세포들은 유문부에 국한되어 다수 관찰되었다. 그러나 PP 면역반응세포들은 전 위장관에 걸쳐 관찰되지 않았다. 결론적으로 ICR 마우스의 위장관내분비세포의 부위별 분포 및 상대적 빈도는 다른 포유동물과 유사하게 관찰되었으나, 일부 특이한 양상을 나타내기도 하였다.

• 대한수의학회지
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• 제43권3호
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• pp.339-347
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• 2003

The regional distribution and relative frequency of some endocrine cells in the pancreas of the Korean aucha perch, Coreoperca herzi Herzenstein belonging to the family Serranidae in order Perciformis, were observed using specific mammalian antisera against serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP) by peroxidase antiperoxidase (PAP) method. The pancreas was divided into four portions (principal and secondary islets, exocrine and pancreatic duct regions). In addition, the pancreatic islet regions were further subdivided into three regions (central, mantle and peripheral regions). Spherical to spindle or occasionally round to oval immunoreactive (IR) cells were demonstrated in the pancreatic islets and exoccrine portions, but no cells were detected in the pancreatic duct portions. In the principal islets, serotonin-IR cells were not detected but most of insulin-IR cells were located in the central regions and they were also demonstrated in the mantle and peripheral regions in moderate and rare frequencies, respectively. Glucagon- and hPP-IR cells were mainly situated in the mantle regions but the cells were also demonstrated in the peripheral regions in relatively lower frequency. Somatostatin-IR cells were evenly distributed in the central and mantle regions in a few frequency and cells were also demonstrated in the peripheral regions in rare frequency. Cell clusters were consisted of hPP-IR cells that were situated in the peripheral to mantle regions. In the secondary islet portions, serotonin-IR cells were randomly distributed throughout the whole pancreatic islet regions but lower frequency was detected in the peripheral regions compared to that in central and mantle regions where cells were detected in a few frequency, respectively. Insulin-IR cells were restricted to the central regions in numerous frequency and glucagon-IR cells were evenly distributed in the mantle and peripheral regions in moderate frequencies, respectively. Somatostatin-IR cells were observed in the central and mantle regions in moderate and a few frequencies, respectively. In addition, hPP-IR cells showed similar distributional patterns to those of glucagon-IR cells except cells were also located in the central regions in rare frequency. In the exocrine portions, only glucagon- and hPP-IR cells were demonstrated in rare and a few frequencies, respectively. In conclusion, the regional distribution and relative frequency of pancreatic endocrine cells of the Korean aucha perch showed general patterns, which were observed in other teleost. However, some species-dependent different distributional patterns and/or relative frequencies were also demonstrated especially to serotonin-IR cells. In pancreas of the Korean aucha perch, insulin-IR cells were the most predominant cell type followed by glucagon-, somatostatin-, hPP- and serotonin-IR cells.

• 대한수의학회지
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• 제32권4호
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• pp.497-510
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• 1992

This study was attempted to comparative investigate the types and regional distribution of the endocrine cells in several vertebrates immunohistochemically using seven antisera. From carp pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were mainly distributed at the periphery and a few cells occupied the central region of the islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, and som - and BPP-immunoreactive cells were located at the central region. From frog pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were distributed at the periphery of the islets, and glucagon- and BPP-immunoreactive cells were found as single cell or as small groups located between the pancreatic acini. From snake pancreas could be observed 3 types which are insulin-, glucagon- and som -immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, and they also were scattered at the periphery of the large islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, whereas som-immunoreactive cells were occupied the central region. From Ogolgae pancreas could be observed 4 types which are insulin-, glucagon-, som-and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, but at the periphery of the large one. Glucagon- immunoreactive cells were distributed at the periphery of the small islets and in the large islets showed scattering entired. Som-immunoreactive cells were distributed at the periphery of the small islets and in the large islets were located at the central region. A small numbers of BPP-immunoreactive cells were located at the periphery of the small islets and the exocrine regions. From the pancreas of the Korean native goat could be observed 6 types which are insulin-, glucagon-, som-, BPP-, 5-HT- and porcine-CG-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were located at the periphery of the islets, but a tew were scattered at the central region of islets and in the epithelium of the secretory duct. Glucagon-, BPP-, 5-HT- and porcine CG-immunoreactive cells were distributed at the periphery of the islets. These findings indicated that the regional distribution patterns and cell types of pancreatic endocrine cells in vertebrates varies considerably among phylogenetically different vertebrates.

• The Korean Journal of Physiology and Pharmacology
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• 제15권3호
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• pp.137-142
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• 2011

Extremely low frequency magnetic fields (ELF-MF) have the ability to produce a variety of behavioral and physiological changes in animals. The stomach, as the most sensitive part of the neuroendocrine organ of the gastrointestinal tract, is crucial for the initiation of a full stress response against all harmful stress. Thus, the purpose of this study was to examine whether ELF-MF stimuli induce changes in the activity of neuroendocrine cells, considering their involvement in endocrine or paracrine effect on surrounding cells. The exposure to ELF-MF (durations of 24 h and 1 or 2 weeks, 60 Hz frequency, 0.1 mT intensity) altered the distribution and occurrence of gastrin, ghrelin and somatostatinpositive endocrine cells in the stomach of rats. The change, however, in the secretion of those hormones into blood from endocrine cells did not appear significantly with ELF-MF exposure. Comparing with sham control, ELF-MF exposure for 1 and 2 week induced an increase in $BaSO_4$ suspension propelling ratio of gastrointestinal tract, indicating that ELF-MF affects gastrointestinal motility. Our study revealed that ELF-MF exposure might influence the activity of endocrine cells, an important element of the intrinsic regulatory system in the digestive tract. The pathophysiological character of these changes and the mechanism responsible for neuroendocrine cell are still unclear and require further studies.

• 대한수의학회지
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• 제39권3호
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• pp.448-454
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• 1999

The regional distribution and relative frequency of the endocrine cells in the pancreas of the bean goose were investigated by immunohistochemical methods using 6 types of the specific antisera. Spindle shaped serotonin-immunoreactive cells were detected in the exocrine portions. Spherical or spindle shaped glucagon-immunoreactive cells were observed in the exocrine and dark and mammalian type islets. In the dark type islets, numerous cells were dispersed throughout whole islets but they were located in the peripheral regions of the mammalian type islets. No glucagon-immunoreactive cells were detected in light type islets. Round or spherical shaped insulin-immunoreactive cells were observed in the exocrine and dark, light and mammalian type islets. They were observed in the exocrine regions with a few numbers. Extremely rare cells were detected in central portion of the dark type islets but moderate to numerous cells were found in the central regions of the mammalian and light type islets, respectively. Spherical or spindle shaped somatostatin-immunoreactive cells were observed in the exocrine and dark, light and mammalian type islets. A few single cells were detected in the exocrine portions. In the dark type islets, numerous cells were dispersed throughout whole islets but a few to moderate numbers of cells were located in the peripheral regions of the light and mammalian type islets. Moderate numbers of the bovine pancreatic polypeptide-immunoreactive cells were found in the exocrine portions with round, spherical or spindle shape. But no bovine Sp-1/chromogranin-immunoreactive cells were observed in this study.