• BMB Reports
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• v.32 no.4
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• pp.345-349
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• 1999

We fused the promoter region of an H3.2 chicken histone gene, whose expression is dependent on the cell cycle, to the 5' coding region of an H3.3 chicken histone gene, which is expressed constitutively at a low level throughout the cell cycle. This fusion gene showed a cell cycle-regulated pattern of expression, but in a different manner. The mRNA level of the fusion gene increase during the S phase of the cell cycle by about 3.7-fold at 6 h and 2.7-fold at 12 h after the serum stimulation. The mRNA level of the intact H3.2 gene, however, increased by an average of 3.6-fold at 6 h and 8.7-fold at 12 h. This different expression pattern might be due to the differences in their 3' end region that is responsible for mRNA stability. The 3' end of the H3.2 mRNA contains a stem-loop structure, instead of a poly(A) tail present in the H3.3 mRNA. We also constructed a similar fusion gene using a H3.3 histone gene whose introns had been eliminated to rule out the possibility of involvement of the introns in cell cycle-regulated expression. The expression of this fusion gene was almost identical to the fusion gene made previously. These results indicate that the promoter region of the H3.2 gene is only partially responsible for its expression during the S phase of the cell cycle.

• SUVANNABHUMI
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• v.8 no.1
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• pp.121-146
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• 2016

Defining key characteristics of Southeast Asia requires historical interpretation. Southeast Asia is a diverse and complicated region, but some of modern history's "grand narratives" serve to unify its historical experience. At a minimum, the modern history of the region involves decisive encounters with universal religions, the rise of Western colonialism, the experience of world wars, decolonization, and the end of the "cycle of violence". The ability of the region's peoples to adapt to these many challenges and successfully build new nations is a defining feature of Southeast Asia's place in the global stage. This paper will begin with a question: is it possible to develop a hermeneutic of "expedience" as a way to interpret the region's history? That is, rather than regard the region from a purely Western, nationalist, "internalist" point of view, it would be useful to identify a new series of interpretative contexts from which to begin scholarly analysis. In order to contextualize this discussion, the paper will draw upon the writings of figures who explored the region before knowledge about it was shaped by purely colonist or nationalist enterprises. To this end, particular attention will be devoted to exploring some of John Furnivall's ways of conceptualizing Southeast Asia. Investigating Furnivall, a critic of colonialism, will be done in relation to his historical situation. Because Furnivall's ideas have played a pivotal role in the interpretation of Southeast Asia, the paper will highlight the intellectual history of the region in order to ascertain the value of these concepts for subsequent historical interpretation. Ultimately, the task of interpreting the region's history requires a framework which will move beyond the essentializing orientalist categories produced by colonial scholarship and the reactionary nation-building narratives which followed. Instead, by beginning with a mode of historical interpretation that focuses on the many realities of expedience which have been necessary for the region's peoples, it may be possible to write a history which highlights the extraordinarily adaptive quality of Southeast Asia's populations, cultures, and nations. To tell this story, which would at once highlight key characteristics of the region while showing how they developed through historical encounters, would go a long way to capturing Southeast Asia's contribution's to global development.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.19 no.2
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• pp.251-259
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• 2010

It is presented in this study a new efficient intelligent machining strategy, which can be used to remove the uncut volume at the boundary region of curved surfaces caused by cutter interference. The geometric form definitions and recognition of topological features of the surface triangulation mesh are used to generate cutter paths along successive and interconnected steepest pathways, that minimize the cusp height left after flat end milling. In order to machine the uncut volume gradually, the z-map cutter centers are adjusted to avoid cutter interference for the 6 kinds of avoidance types. And then, the generative subsequent paths are sequenced to determine the second step cutter paths for the next uncut volume. For the 2 kinds of test models with convex and concave surface region, the implemented software algorithm is evaluated by investigating the residual swelling of uncut volume for each machining step.

• Transactions of the Korean Society of Automotive Engineers
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• v.21 no.5
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• pp.97-103
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• 2013

Crash performance of the straight member was studied by FE analysis. One end of model was fixed and the other end was impacted by 1,000kg rigid mass with velocity of 16.0m/sec. The maximum and mean load were discussed to compare crash performance. The members with various section shapes were analyzed and the flange location was changed. Also, spot weld points were added in the initial buckling region to investigate its effect. Final rectangular section model which has flanges at the center and reinforcement in initial buckling region showed high enhancement in crash performance.

• Journal of Korea Multimedia Society
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• v.25 no.2
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• pp.247-256
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• 2022

Visual tracking of selected target objects is fundamental challenging problems in computer vision. Object tracking localize the region of target object with bounding box in the video. We propose a Siam-FPN based custom fully CNN to solve visual tracking problems by regressing the target area in an end-to-end manner. A method of preserving the feature information flow using a feature map connection structure was applied. In this way, information is preserved and emphasized across the network. To regress object region and to classify object, the region proposal network was connected with the Siamese network. The performance of the tracking algorithm was evaluated using the OTB-100 dataset. Success Plot and Precision Plot were used as evaluation matrix. As a result of the experiment, 0.621 in Success Plot and 0.838 in Precision Plot were achieved.

• Korean Journal of Microbiology
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• v.42 no.3
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• pp.165-171
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• 2006

ERM proteins transfer the methyl group to $A_{2058}$ in 23S rRNA to confer the resistance to MLS (macrolide-lincosamide-streptogramin B) antibiotics on microorganism ranging from antibiotic producers to pathogens. To define the functional role of peptide segment encompassing amino acid residues 1 to 25 in NTER (N-terminal end region) of ErmSF, one of the ERM proteins, DNA fragment encoding mutant protein deprived of that peptide was cloned and overexpressed in E. coli to obtain a purified soluble form protein to the apparent homogeneity in the yield of 12.65 mg per liter of culture. The in vitro activity of mutant protein was found to be 85% compared to wild type ErmSF, suggesting that this peptide interact with substrate to affect the enzyme activity. This diminished activity of mutant protein caused the delayed expression of antibiotic resistance in vivo, that at fIrst cells expressing mutant protein showed the retarded growth due to the antibiotic action but with time cells inhibited by antibiotic gradually recovered the viability to exert the resistance to the same extent as those with wild type protein.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2003.04a
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• pp.223-228
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• 2003

In end milling processes, characterized by use of rotating tools, the underformed chip thickness varies periodically with the phase change of tool. In current study, as a new approach to analyse shear behaviors In the shear plane and chip-tool friction behavior chip-tool contact region during an end milling process. In this approach, an up-end milling process is transformed into an equivalent oblique cutting process. Experimental investigations for two sets of cutting tests i.e.. up-end milling and the equivalent oblique cutting test were performed to verify the presented model.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.04a
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• pp.1003-1008
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• 1997

In this paper, the prediction of cutting force and tool deflection in the ball-end milling process are studied. Identifying various cutting region using Z-map, cutting force in the ball-end milling process can be predicted. Cutting force deflects the tool and the tool deflection changes the cutting force. Tool deflection is included in the cutting force prediction. Tool deflecition also causes machining error of the machined surface. A series of experiments were performed to verify the simulated cutting force and machining error.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.10a
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• pp.465-467
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• 2010

This paper proposes MIMO two-way cooperative relay scheme to optimize the end to end capacity in wireless multi-hop mesh network. The basic idea is to perform data transmission via multi-hop relay nodes, in equidistant topology, this method is quite efficient. However, on one hand this topology is very rare in practical situation, on the other hand, in real practical situation where the topology is most likely non equidistant, the end to end capacity significantly degrades due to bottleneck link caused by uneven SNR. Moreover, the end to end capacity degrades at high SNR due to overreach interference from far nodes existing in multi-hop relay networks. In this paper, MIMO two-way cooperative relay in the region of non equidistant nodes is found efficient to improve the end to end capacity. The proposed scheme is validated using numerical simulation.

• Animal cells and systems
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• v.3 no.4
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• pp.413-415
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• 1999

Our previous report demonstrated that the rhp51$^＋$, a recA and RAD51 homolog of the fission yeast, encodes three transcripts of 1.9, 1.6 and 1.3 kb which have at least six polyadenylation sites. The 3'-end of the gene alone can direct the formation of multiple, discrete 3'ends of the transcripts. To identify the regulatory element required for the 3'-end formation of -rhp51$^＋$ deletion mapping analysis was performed. Northern blot analysis revealed that the 254-bp DNA fragment including 4 distinct poly (A) sites downstream from the Hindlll site, is crucial for normal 3'-end formation. Deletion of the 3'-terminal AU rich region caused appearance of read-through RNA, leading to enhancement of survival rate of the rhp51 deletion mutant in response to DNA damaging agent, methylmethane sulfonate (MMS). The results imply that the rhp51$^＋$ system may be useful for molecular analysis of the 3'-end formation of RNA in the fission yeast.