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Characterization of ovarian culture in vitro and sex steroids in vivo by recombinant eel gonadotropin treatments in the eel Anguilla japonica

  • Kim, Dae-Geun;Kim, Jung-Hyun;Baek, Hea-Ja;Kim, Shin-Kwon;Min, Kwan-Sik;Kim, Dae-Jung
    • Fisheries and Aquatic Sciences
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    • v.25 no.1
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    • pp.12-19
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    • 2022
  • In the present study, we investigated the effects of recombinant eel gonadotropins (rec-GTHs) on maturation induction in immature ovarian culture in vitro and sex steroid hormones in vivo in the Japanese eel Anguilla japonica. To study the in vitro effects of rec-GTHs on estradiol-17β (E2) production in immature ovarian tissues, ovarian tissues were incubated with different doses of rec-follicle-stimulating hormone (rec-FSH) or rec-luteinizing hormone (rec-LH). The results revealed that the E2 levels in the rec-FSH (0.1, 0.5, or 1 ㎍/mL)- and rec-LH (0.1 or 0.5 ㎍/mL)-treated groups were significantly higher than those in the female eels from the control group. Furthermore, to investigate the in vivo effects of rec-GTHs on the gonadosomatic index (GSI) and plasma sex steroid hormone levels, the eels were injected intraperitoneally with eel's ringer (control), salmon pituitary extract (SPE; for female eels), human chorionic gonadotropin (hCG; for male eels), rec-FSH, rec-LH, and rec-FSH + rec-LH once a week. The results revealed that except for the SPE and the hCG groups, none of the groups exhibited a significant difference in GSI values. However, in vivo plasma E2 levels increased at the end of 4 weeks after rec-FSH treatment in female eels. Based on these results, it is suggested that rec-GTHs may have a positive effect on sexual maturation in female eels; however, further studies on complementary rec-protein production systems and additional glycosylation of rec-hormones are needed to elucidate hormone bioactivity in vivo and in vitro.

An Integrative Review of Meditation Program for Stress Relief of Nurses (국내 간호사의 스트레스완화를 위한 명상프로그램에 관한 통합적 고찰)

  • Park, Ye-Ri-Ja
    • The Journal of the Korea Contents Association
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    • v.22 no.1
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    • pp.557-570
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    • 2022
  • The purpose of this study was to review meditation programs for stress relief of Korean nurses. Whittmore and Knafl's integrative review methods were used. The search yielded 97 articles, 7 of which met the criteria and were included in the review. One of the selected studies was a randomized controlled quasi-experimental design and six were a non-equivalent control group pretest-posttest design. Meditation programs varied from 5 to 8 sessions. All of the meditation programs measured the effect on stress as various meditation programs. However, there was one study confirming the degree of stress management maintenance after the end of the meditation program. Although there are contents to strengthen the practice and will of nurses, it was confirmed that it was difficult to evaluate sincerity. Therefore, a highly accessible program for nurses in various clinical situations and strategies to strengthen participants' practice and will needs to be included in the meditation program content.

Effect of sodium stearoyl-2-lactylate (PROSOL®) supplementation on growth performance and nutrient digestibility in growing pigs

  • Vetriselvi Sampath;In Ho Kim
    • Korean Journal of Agricultural Science
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    • v.49 no.1
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    • pp.45-50
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    • 2022
  • The aim of this study is to evaluate the effects of supplementation of low-energy diets with PROSOL® (sodium stearoyl-2-lactylate) as an emulsifier on the growth performance and nutrient digestibility of growing pigs. A total of 120 crossbred ([Landrace × Yorkshire] × Duroc) growing pigs with an average initial body weight of 23.80 ± 4.87 kg were used in a 56-day feeding trial and were sorted into three dietary treatments. Each treatment utilized eight replications with five pigs per pen. The dietary treatments were as follows: PC (high, net energy diet [NE] = 2,488 kcal·kg-1 [weeks 1 to 4] and NE = 2,477 kcal·kg-1 [weeks 5 to 8]); NC (low, NE = 2,472 kcal·kg-1 [weeks 1 to 4] and NE = 2,468 kcal·kg-1 [weeks 5 to 8]); NC1 (NC + 0.05% PROSOL® emulsifier). Growth performance outcomes were measured at the initial point and at weeks 4 and 8 while nutrient digestibility parameters were measured at weeks 4 and 8. From weeks 1 - 8, body weight and the average daily gain of the NC1 group pigs showed significant increases (p < 0.05) compared to those of the PC and NC treatment groups. At the end of the trail, pigs fed a diet containing low net energy with 0.05% of the emulsifier showed reduced feed conversion ratio (FCR) levels. However, the average daily feed intake and nutrient digestibility of dry matter and nitrogen remained unaffected throughout the experiment. In summary, the addition of a low-net-energy diet with 0.05% sodium stearoyl-2-lactylate as an emulsifier enhanced the body weight and daily gain with no adverse effects on nutrient digestibility in growing pigs.

Hands-on Education Module for Modular Construction, 3D Design, and 4D Schedule

  • Kithas, Kyle A.;Choi, Jin Ouk
    • International conference on construction engineering and project management
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    • 2022.06a
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    • pp.484-491
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    • 2022
  • A paradigm shift in teaching modular construction in higher education and K-12 is proposed as a means to increase the future adoption of the modular construction technique. To this effect, a new education module is presented to STEM educators. This education module is based on LEGOs and directed towards educators in the architecture, engineering, and construction (AEC) industry. The main objectives of the education module are to increase interest and knowledge of modular construction, acknowledge the benefits of using 3D design with 4D scheduling, and create a simulating hands-on educational opportunity. The education module is designed to allow participants to experience a hands-on simulation of modular construction and stick-built construction through building a LEGO project. Participants are challenged to find the advantages and disadvantages in both construction systems first-hand and record their findings. Results are presented from the preliminary testing of this education model on a group of construction management students at the University of Nevada, Las Vegas. Overall, the survey results showed that the LEGO education module was successful at achieving the project's three main objectives: 1) increasing the participants' interest and knowledge of modular construction through an interactive project; 2) increasing the participants' understanding of the benefits of 3D design with 4D scheduling over the use of 2D drawings; and 3) creating a simulating hands-on educational opportunity to help participants compare modular construction to stick-built construction. In the end, this proposed a new LEGO education module addressing the problems identified from this study with more participants.

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Preliminary Teachers' Perception of Elementary Classes Based on Edutech: focusing on K Teachers' College (에듀테크 기반 초등수업에 대한 예비교사들 인식: K교육대학교의 사례를 중심으로)

  • Tecnam Yoon
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.2
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    • pp.127-132
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    • 2023
  • The rapid development and progress of information and communication technology are bringing about changes in the field of education, and innovative methods in teaching-learning have been introduced and applied a lot in the actual field starting with the pandemic. Reflecting this trend, the purpose of this study is to examine the perception of prospective elementary school teachers on the use of edutech, which is widely used in the educational field after COVID-19. To this end, I analyzed the results of 7 major ideas selected at the Edutech Utilization Competition held by K University of Education and the focus group interview conducted with 2 pre-service teachers. As a result of the analysis, it was found that prospective elementary school teachers valued the necessity and importance of edutech-based classes. In addition, they were positively aware of the educational effect of edutech-based classes. To sum up, it could be predicted that edutech classes are also linked to the policies and plans of the Ministry of Education, and will play an important role in effectively guiding future learners while achieving the goals set by the curriculum.

Effects of Supplemental Recombinant Bovine Somatotropin (rbST) and Cooling with Misters and Fans on Renal Function in Relation to Regulation of Body Fluids in Different Stages of Lactation in Crossbred Holstein Cattle

  • Boonsanit, D.;Chanpongsang, S.;Chaiyabutr, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.3
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    • pp.355-365
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    • 2010
  • The aim of this study was to investigate the effect of supplemental recombinant bovine somatotropin (rbST) and cooling with misters and fans on renal function in relation to regulation of body fluids in different stages of lactation in crossbred Holstein cattle. Ten, 87.5% crossbred Holstein cattle were divided into two groups of 5 animals each, housing in a normal shaded barn (NS) and in a shaded barn with a mister-fans cooling system (MF). The experiment in each group was divided into 3 phases, early- (Day 75 postpartum), mid- (Day 135 postpartum), and late stage of lactation (Day 195 postpartum). The pre-treatment study was conducted on the starting day of each stage of lactation and the treatment study was performed after the end of the pre-treatment, during which the animal was injected with 500 mg of rbST (POSILAC) every 14 days for three times. During the study, ambient temperature at the hottest period daily in the MF barn was significantly lower, while relative humidity was higher than that of the NS barn. The temperature humidity index (THI) in both barns ranged from 79-85 throughout the periods of study. Cows in the MF barn showed a lower rectal temperature and respiration rate as compared with cows in the NS barn. The effect of rbST administration increased both rectal temperature and respiration rates of cows housed in either the NS or MF barn. Milk yield significantly increased in cows treated with rbST in all stages of lactation. Increases in mammary blood flow, accompanied by increases of total body water (TBW), extracellular fluid (ECF), blood volume (BV) and plasma volume (PV), were observed in both groups of cows receiving rbST in all stages of lactation. No alterations of renal blood flow and glomerular filtration rate were observed in cows receiving rbST, but decreases in urinary excretion and fractional excretion of sodium, potassium and chloride ions appeared to correlate with reduction in the rate of urine flow and osmolar clearance during rbST administration. These results suggest that the effect of rbST supplementation to cows housed either in NS or MF barns on body fluid volume expansion is attributable to changes in the rate of electrolyte excretion by the kidney. The increased availability of renal tubular reabsorption of sodium, potassium and chloride ions during rbST treatment was a major factor in retaining body water through its colligative properties in exerting formation of an osmotic force mechanism.

Feeding Effect of an Anabolic Steroid, Nandrolone, on the Male Rat Testis

  • Lee, Dong-Mok;Min, Tae-Sun;Choi, In-Ho;Cheon, Yong-Pil;Chun, Tae-Hoon;Park, Chang-Sik;Lee, Ki-Ho
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.12
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    • pp.1566-1577
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    • 2010
  • Nandrolone, 19-nortestosterone, is a synthetic androgenic-anabolic steroid promoting muscle growth. Nandrolone is also present in pig meat and sera at non-negligible levels. A number of scientific reports have suggested a positive relationship between incidence of infertility and increased meat consumption in humans. The present study was designed to determine out the effect of feeding nandrolone on the testis of the male reproductive tract. Mixtures of food and nandrolone at different concentrations (0.005 ppm and 0.5 ppm) were supplied to pubertal male rats for 6 weeks. Body weight was recorded every week during the entire experimental period. At the end of the treatment, the testis, epididymis, and epididymal fat were collected and weighted. Sperm numbers in the caudal epididymis were counted. Differential gene or protein expression of steroidogenic enzymes in the testes among experimental groups was determined by semi-quantitative real-time PCR or western blotting analysis, respectively. Histological changes of the testis induced by nandrolone treatment were examined by hematoxylin and eosin staining. Immunohistochemical analysis was employed to detect changes in the localization of steroidogenic enzymes in the testes among experimental animals. There were no significant changes on body, testis, epididymis, and epididymal fat weights among experimental groups. A significant increase of caudal sperm number was found in the 0.5 ppm nandrolone-treated group. Histological examination of the testes noted a high frequency of germ cell sloughing in seminiferous tubules of 0.5 ppm nandrolone-treated rats. Even though transcript levels of $3{\beta}$-hydroxysteroid dehydrogenase (HSD) I, $17{\beta}$-HSD4, and $17{\alpha}$-hydroxylase were influenced by nandrolone treatments, protein levels of all molecules examined in the present study were not significantly affected. Immunohistochemical analysis showed no visible changes in the localization of steroidogenic enzymes in the testes among experimental groups. The current study showed that oral intake of nandrolone in male rats for 6 weeks did not cause significant damage to the testis. It is considered that a feeding effect of nandrolone on male fertility would not be remarkable.

Comparative Analysis on Health Care Expenditure and Its Improvement Effect for Social Welfare: An Application of Principal Component Analysis (보건의료비의 사회후생 증대효과 비교분석: 주성분분석을 중심으로)

  • Rhee, Hyun-Jae
    • The Journal of the Korea Contents Association
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    • v.13 no.2
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    • pp.378-389
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    • 2013
  • Health care expenditure might be understood as a core source of social welfare. It is mainly due to the fact that the health care expenditure is closely related to the level of social welfare. And, therefore, the paper is basically designed to investigate the effect of social welfare improvement resulted from an increasing in per capita, private, public, and total health care expenditures during 1995-2009 periods. Empirical evidence reveals that the improvement of social welfare in middle income group reveals the highest improvement in social welfare, and the other income groups maintain a quite similar pattern in improvement of social welfare. As far as category of health care expenditure concerns, the effect of social welfare improvement by per capita health care expenditure is in general lower in all case. And, private sector achieves the highest improvement of social welfare in comparing with public and total health care expenditures. To this end, it could be tentatively concluded that the private health care expenditure has to be substituted for the public sector to induce market-oriented operational system. Accordingly, it would be better to build an institutional basis more elaborately in setting up the market system.

The Impacts of the Service Quality of Coffee Shop Adapting the CoffeeSERV on Customer's Perceived Value, Customer Satisfaction, Behavioral Intention: Focusing on Regulatory Focus Theory (CoffeeSERV측정모형을 활용한 커피전문점 서비스품질의 가치지각, 고객만족, 행동의도의 영향관계 연구: 조절초점동기의 조절효과를 중심으로)

  • KANG, Hwa-Seok
    • The Korean Journal of Franchise Management
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    • v.10 no.3
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    • pp.37-52
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    • 2019
  • Purpose - This study examined the relationship between service quality, perceived value, customer satisfaction and behavioral intention of coffee shop using CoffeeSERV scale. In this model, CoffeeSERV scale consists of fundamental characteristics, physical environment, confidence, beverage characteristics, and representation factors. In particular, this study tried to demonstrate the moderating effect of customer's regulatory focus orientation among in the relationships between service quality, perceived value, customer satisfaction and behavioral intention. Research design, data, and methodology - This study intends to expand the existing service quality research by using the coffee shop service quality measurement tool developed by domestic researchers. I wanted to find some implications for the trend. In particular, this study applied the regulatory focus theory to identify individual differences of customers regulatory focusing motivation. In order to verify several hypotheses, the data were 227 college students and analyzed with SPSS/PC 21.0 and SmartPLS 3 program. The moderating role of customer's regulatory focusing motivation was tested using multi-group analysis with SmartPLS 3 program. Results - The resutls are as follows. First, the fundamental characteristic factors only had a significant influence on the utilitarian value perception, but in the hedonic value perception, all other service factors except for the beverage characteristic had a statistically significant effect. Second, utilitarian and hedonic value had significant effects on customer satisfaction. Third, customer satisfaction had a significant effect on behavioral intention. Finally, the regulatory focus orientation played a moderating role in the relationship between beverage characteristic - utilitarian value, representation - utilitarian value, fundamental characteristic - hedonic value, physical environment - hedonic value, confidence - hedonic value, and utilitarian value - behavioral intention. Conclusions - The results of this study show that the various service quality factors that make up the CoffeeSERV scale have different effects on utilitarian and hedonic value. This means that perceived benefits from product and service experience have different impacts on the customer's experience. Therefore, marketers should identify the impacts of service quality dimension that customers who use coffee shops consider important, understand the impact process of these quality factors on experience value, customer satisfaction, and behavioral intention, and allocate limited marketing budget. The results also show that it is possible to establish differentiatied response strategies using customer's regulatory focus orientation to find ways to enhance utlitarian and hedonic value, customer satisfaction, and behavioral intention using various Coffeeshop service quality factors. At the end of this paper, some limitations and future research directions were suggested.

A Study on the Effect of Life Behavior and Socio-environmental Satisfaction on Life Satisfaction of the Elderly People with or without Hearing Loss (난청 여부에 따른 노인의 생활행태와 사회환경만족도가 삶의 만족도에 미치는 영향 연구)

  • Jeong, Su-yeon;Byun, Jae-hee;Jung, Deuk;Jo, Changik
    • Journal of Industrial Convergence
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    • v.20 no.9
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    • pp.99-107
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    • 2022
  • This study was conducted to empirically analyze the effect of life behavior and socio-environmental satisfaction on life satisfaction of the elderly with or without hearing loss. To this end, 3,071 elderly people of 「the Survey on the Actual Condition of the Elderly」 in 2020 were set as the subjects. After controlling for their demographic variables, the multiple regression model showed that the elderly with hearing loss had different factors of life behavior and socio-environment satisfaction that affected their life satisfaction compared to those without. As a result, the sub-factors of life behavior in the elderly with hearing loss, such as economic activities, social group activities, the use of the center, and the sub-factors of social environment satisfaction, such as family satisfaction and environmental satisfaction, had a positive (+) effect on life satisfaction. Especially, the elderly with hearing loss had more limitations in both the area of living behavior and the area of social environment satisfaction than the elderly without hearing loss. Therefore, in order to improve the life satisfaction of the elderly with hearing loss, it is suggested that the government and local governments should simultaneously supplement welfare policies and facilities for the elderly with hearing loss.